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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Vegetalika Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmiah Aplikasi Isotop Radiasi Jurnal Hortikultura AGRIVITA, Journal of Agricultural Science Jurnal Agro Jurnal AGROSAINS dan TEKNOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biologi Tropis Journal of Tropical Crop Science AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Molekul: Jurnal Ilmiah Kimia Menara Perkebunan Jurnal Farmamedika (Pharmamedica Journal) Akta Agrosia Agrotech Journal

Syarifah Iis Aisyah

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian BogorJl. Meranti, Kampus IPB Darmaga, Bogor, 16680, Indonesia

Author-ID : 223003

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Respon Kerapatan Stomata dan Kandungan Klorofil Padi (Oryza sativa L.) Mutan terhadap Toleransi Kekeringan Hasna Dama; Syarifah Iis Aisyah; Azri Kusuma Dewi; S Sudarsono
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 16, No 1 (2020): JUNI 2020
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/jair.2020.16.1.5689

Perakitan varietas padi toleran cekaman kekeringan dengan teknik pemuliaan mutasi mampu meningkatkan keragaman genetik tanaman sehingga memberi peluang untuk mendapatkan genotipe mutan yang toleran dengan mengetahui respon kerapatan stomata dan klorofil daun pada suatu tanaman. Penelitian ini bertujuan untuk melihat hubungan antara kerapatan stomata dan kandungan klorofil dengan ketahanan terhadap kekeringan pada genotipe padi mutan. Sampel menggunakan salah satu genotipe, daun yang digunakan adalah daun kedua dari daun bendera, perhitungan jumlah stomata dilakukan pada luas bidang pandang 40x dengan perhitungan jumlah stomata dibagi dengan satuan luas bidang pandang dan untuk klorofil daun diamati menggunakan klorofil meter (SPAD). Hasil penelitian menunjukkan bahwa genotipe padi mutan menunjukkan ketahanan terhadap cekaman kekeringan dengan respon kerapatan stomata paling tinggi dan skor penggulungan daun terkecil.

Penentuan Dosis Iradiasi Sinar Gamma dalam Meningkatkan Keragaman untuk Perbaikan Karakter Kuantitatif Bawang Merah (Allium cepa var. aggregatum) Wulan Septiningtyas Kurniajati; Sobir Sobir; Syarifah Iis Aisyah
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 16, No 2 (2020): Desember 2020
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/jair.2020.16.2.5962

Perbanyakan bawang merah (Allium cepa var. aggregatum) secara luas menggunakan umbi, oleh karena itu mutasi merupakan metode yang efektif untuk menginduksi keragaman genetik. Tujuan dari penelitian ini adalah untuk mendapatkan informasi mengenai radiosensitivitas dan menginduksi keragaman genetik pada bawang merah varietas Bima Brebes menggunakan iradiasi sinar gamma. Bahan tanam dikelompokkan berdasarkan asal material tanaman, yaitu umbi dan biji. Dosis iradiasi biji adalah 0-1000 Gy dengan rentang 100 Gy, sedangkan pada umbi adalah 0, 1, 2, 4, 6, 8, dan 10 Gy. Radiosensitivitas umbi bawang merah lebih tinggi dibandingkan biji. Nilai LD50 bawang merah asal umbi adalah 7,55 Gy dan asal biji adalah 75,26 Gy. Iradiasi mampu menstimulasi pertumbuhan tanaman pada dosis yang mendekati LD20 pada umbi, yaitu pada nilai 2 Gy. Keragaman yang terbentuk tersebar secara acak pada berbagai dosis iradiasi dan karakter pengamatan.

Sensitivity in Callus Tissue of Plectranthus amboinicus (L.) through Mutation Induction with Colchicine Kunto Wibisono; Syarifah Iis Aisyah; Waras Nurcholis; Sri Suhesti
AGRIVITA, Journal of Agricultural Science Vol 44, No 1 (2022)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v44i1.3058

Mutation induction by colchicine is one method to improve plant genetic diversity (polyploidy). This study aims to 1) obtain growth regulators composition suitable in inducing callus, 2) obtain the LC50 value in callus of P. amboinicus, 3) to determine the effect of colchicine on callus growth, 4) to get shoots (putative mutant) varian, regenerating from indirect organogenesis (callus) in MV1. The study is conducted at the Laboratory of Agricultural Seed Management, Plantation Research, and Development Center from June 2018 to June 2019. The study uses a complete randomized design with four treatments and three replications. Explants use ware the nodes. Callus induction uses MS0 medium with 0.5 ppm 2.4-D, 2 ppm 2.4-D, 0.5 ppm 2.4-D + 1 ppm BAP, and 0.5 ppm 2.4-D + 0.5 ppm TDZ treatment. Mutation induction was performed using colchicine concentration at 0%, 0.02%, 0.04%, and 0.06% treatments. The regeneration media is an MS0 medium. The results show the best combination of plant growth regulators to induce the callus is 0.5 ppm 2.4-D. The LC50 colchicine concentration is at 0.0275%. However, this phenotype is reversible. An additional subculture period is required to move to normal morphology

STUDI RADIOSENSITIVITAS DAN ANALISIS KERAGAMAN M1 KACANG TUNGGAK (Vigna unguiculata L) HASIL INDUKSI MUTASI Yukarie Ayu Wulandari; Sobir Sobir; Syarifah Iis Aisyah
Jurnal AGROSAINS dan TEKNOLOGI Vol 4, No 1 (2019)
Publisher : Fakultas Pertanian - UMJ

Kacang tunggak sebagai salah satu kacang indigenous potensi untuk substitusi kedelai sebagai bahan dasar pembuatan tempe dan tahu. Sumber daya genetik kacang tunggak perlu ditingkatkan keragamannya sebagai sumber plasma nutfah untuk perakitan varietas unggul baru. Induksi mutasi iradiasi sinar gamma pada kacang tunggak diharapkan dapat meningkatkan keragaman. Penelitian dilaksanakan dengan iradiasi biji kacang tunggak genotipe KM-4 dengan dosis 0, 200, 400, 600 dan 800 Gy dan dianalisis untuk memperoleh LD50 dan dilakukan iradiasi kembali dengan dosis 0, LD50-100, LD50-50, LD50, LD50+50 dan LD50+100 Gy. Karakter yang diamati meliputi tinggi tanaman, lebar tajuk, panjang tangkai, panjang daun, lebar daaun, periode panen, panjang polong, jumlah biji/polong, berat biji/tanaman dan kandungan protein biji kacang tunggak. Hasil penelitian menunjukkan bahwa nilai LD50 tanaman kacang tunggak adalah 724,84 Gy, iradiasi sinar gamma pada dosis750 Gy menghasilkan keragaman tertinggi dan iradiasi sinar gamma tidak meningkatkan keragaman terhadap kandungan protein biji kacang tunggak.

ANALISIS LINTAS PERTUMBUHAN DAN PRODUKSI TERHADAP PROTEIN KACANG TUNGGAK (Vigna unguiculata L) GENERASI M2 Yukarie Ayu Wulandari; Sobir Sobir; Syarifah Iis Aisyah
Jurnal AGROSAINS dan TEKNOLOGI Vol 6, No 1 (2021)
Publisher : Fakultas Pertanian - UMJ

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jat.6.1.7-14

Cowpea (V. unguiculata L) can be developed as a source of vegetable protein because it contains relatively high protein and high lysine. The protein content in seeds is an accumulation of character components that play a role in protein formation, whose relationship can be predicted using cross correlation and analysis coefficients. The study was carried out in the experimental garden of Pasir Kuda PKHT of IPB in February - May 2018. Protein analysis is carriedout in the Testing Laboratory of the Department of Agronomy and Horticulture, Faculty of Agriculture, IPB in June - July 2018. The study was conducted using 30 putative mutant test genotypes of M2 generation result from the mutation of gamma ray irradiation and KM4 genotype as a comparison which was repeated three times. The protein content of the seeds was analyzed using the Kjeldahl method and then analyzed the variance, correlation coefficient and path. The results showed that M2 generation putative mutants showed diversity for the character of protein content in cowpea seeds. The protein content in cowpea seeds can be predicted through the character of plant height and number of branches.

ANALISIS KERAGAMAN DAN KEKERABATAN KACANG TUNGGAK (Vigna unguiculata L) GENERASI M2 Yukarie Ayu Wulandari; Sobir Sobir; Syarifah Iis Aisyah
Jurnal AGROSAINS dan TEKNOLOGI Vol 5, No 1 (2020)
Publisher : Fakultas Pertanian - UMJ

Cowpea (V. unguiculata L) has great potential as a nutritious food as a substitute for soybeans because it contains sufficient protein and low fat content. The diversity of cowpea is low so that need to increase diversity through the mutation induction of gamma ray irradiation. The study was carried out in the experimental garden of Pasir Kuda PKHT of IPB in February - May 2018 using a design of augmented in the Complete Group Design in a Randomized. The study was carried out using 90 putative mutant genotypes of M2 generation as the test genotype and KM4 genotype as a comparison which was repeated 10 times. The results showed that the M2 generation putative mutants showed diversity in the qualitative and quantitative characters of cowpea. High genetic diversity is shown in the character of plant height, harvest period, number of seeds / pods and weight of cowpea seeds / plants and high broad mean heritability values obtained on the character of stem length, flowering age, number of seeds / pods and weight of beans / plant nuts arrears. The result of kinship analysis showed thirteen different putative mutant genotypes with KM4 genotypes, namely T6599P, T8028P, T7525P, T7551P, T7520P, T6574P, T6533P, T7058P, T6577P, T6591P, T7062P, T7069P and T6561.

Optimization of Total Flavonoids Extraction and Α-Glucosidase Inhibitory Activity from Plectranthus amboinicus (Lour.) Spreng. Leaves using the Simplex-Centroid Design Kunto Wibisono; Syarifah Iis Aisyah; Sri Suhesti; Waras Nurcholis
Molekul Vol 14, No 2 (2019)
Publisher : Universitas Jenderal Soedirman

The leaves of Plectranthus amboinicus (Lour.) Spreng. (Lamiaceae) is a perennial plant that contained flavonoids compounds with have antidiabetic properties. The aim of the work was to select the best solvent for extraction of the flavonoid compounds from torbangun leaves. A statistical simplex-centroid mixture design for the water, ethanol, and hexane solvents has been used to the extraction of extract yield, flavonoids content, and inhibitory α-glucosidase activity of torbangun leaves. The ethanol-water extracts showed the highest extract yield with value of 15.92%. The ethanol-hexane extracts presented the highest total flavonoids content, 96.30 mg QE/g, followed by hexane extract (82.91 mg QE/g). The ethanol extract recorded higher inhibitory of α-glucosidase activity, with value of 25.53%, followed by hexane extract (25.21%), and ethanol-water extract (23.02%). The weak correlation between the α-glucosidase inhibition and total flavonoids content showed that flavonoids contribute non-significantly to the α-glucosidase activity.

Induksi mutasi Stevia rebaudiana dengan perendaman kolkisin secara in vitro (Induced mutation of Stevia rebaudiana through colchicine soaking in vitro) Masna Maya SINTA; Ni Made Armini WIENDI; Syarifah Iis AISYAH
E-Journal Menara Perkebunan Vol 86, No 1 (2018): April, 2018
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Stevia rebaudiana Bert. is a plant producing steviol glycosides that have 200-300 times sweeter than sucrose. These steviol glycosides are produced in the leaves and then spread to all parts of the plant including stems. The use of superior stevia planting material is important for stevia sugar industry. One of the stevia breeding programme is to increase genetic diversity through colchicine soaking to produce polyploid plants. Polyploid plants usually have higher vigor than diploid plants. The purpose of this research was to induce genetic diversity of stevia through colchicine soaking in vitro. Single nodes of sterile stevia clone BS were soaked in colchicine at the concentration of 0.01; 0.02; 0.04; 0.08 and 0.1% for 48 and 72 hours, and in sterile aquadest as a control. Plantlet subcultures were done until MV4 (mutant vegetative 4). Putative mutants were observed by plantlet vigor and stomata analyses on MV5. Vigor of plantlets was observed by counting the number of leaves, nodes, roots, fresh weight and dry weight of the plantlet. Stomata analysis was performed by calculating stomata density, stomata size and chloroplast number in stomata guard cells. Results showed that colchicine soaking treatment increased significantly fresh weight and dry weight of putative mutants. Colchicine soaking treatment increased chloroplast number on stomata guard cell and stomata size, but decreased stomata density. Stevia soaked in colchicine for 48 hours at concentration 0.01-0.04% produce putative mutants with high chromosome numbers. [Key words: poliploidy, stomata, chloroplast, mutant]AbstrakStevia rebaudiana Bert. merupakan tanaman penghasil glikosida steviol yang memiliki tingkat kemanisan 200-300 kali lebih tinggi dibandingkan sukrosa. Glikosida steviol ini diproduksi di daun yang kemudian disalurkan ke bagian tanaman lainnya termasuk batang. Penggunaan klon terbaik stevia merupakan salah satu kunci penting keberhasilan industri gula stevia. Salah satu program pemuliaan tanaman stevia adalah meningkatkan keragaman tanaman melalui mutasi dengan kolkisin sehingga menghasilkan tanaman poliploid. Tanaman poliploid umumnya memiliki vigor lebih baik dibandingkan tanaman diploid. Tujuan dari penelitian ini adalah untuk meningkatkan keragaman stevia melalui peren-daman kolkisin in vitro. Buku tunggal steril stevia klon BS direndam dalam kolkisin dengan konsentrasi 0,01; 0,02; 0,04; 0,08 dan 0,1% selama 48 dan 72 jam dengan perendaman dalam air steril sebagai kontrol. Sub kultur dilakukan hingga MV4 (mutan vegetatif 4). Pengamatan mutan putatif dilakukan meliputi analisis morfologi dan stomata pada MV5. Analisis morfologi dilakukan dengan mengamati jumlah daun, buku, akar, bobot basah serta bobot kering planlet. Analisis stomata dilakukan dengan menghitung kerapatan stomata, ukuran stomata serta jumlah kloroplas pada sel penjaga stomata. Hasil menunjukkan bahwa perendaman stevia pada kolkisin meningkatkan bobot basah serta bobot kering stevia in vitro. Perlakuan perendaman kolkisin meningkatkan jumlah kloroplas pada sel penjaga stomata serta ukuran stomata namun menurunkan kerapatan stomata. Perendaman stevia selama 48 jam pada konsentrasi kolkisin 0,01-0,04% menghasilkan mutan putatif dengan jumlah kromosom tertinggi.[Kata kunci: poliploidi, stomata, kloroplas, mutan]

Analisis komparatif kandungan metabolit pada daun mutan tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng.): Analisis komparatif kandungan metabolit pada daun mutan tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng.) Syarifah Iis Aisyah; Henny Rusmiyati; Dewi Sukma; Rizal Damanik; Waras Nurcholis
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 4 No 1 (2020): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v4i1.109

Torbangun (Plectranthus amboinicus) is a medicinal plant belonging to the Lamiaceae family. In Batak society, the torbangun leaves are consumed to increase breast milk production. The phytochemical variability can be improved by gamma-ray irradiation for plant breeding programs. In this work, a non-targeted metabolomics method was followed to evaluate the compounds profile of irradiated mutant and wild-type on leaves of P. amboinicus. GC-MS analyses evaluated the mutant putative (derived from gamma-ray irradiation) and wild-type plantlets. The resulting of GC-MS data were analyzed with chemometric analysis using hierarchical cluster analysis (HCA). The chemical diversity was showed in mutant and wild-type P. amboinicus. The major metabolite in plantlet wild-type were hydroxymethylfurfural (35.15%), brevifolin (7.69%), 3-Dihydro-3,5-Dihydroxy-6-Methyl-4H-Pyran-4-one (DDMP) (13.13%), stigmasterol (6.51%) and ferruginol (8.63%). However, the compound of DDMP (19.57%), neophytadiene (8.47%), linolenic acid (9.10%), ferruginol (7.61%), stigmasterol (14.14%) and gamma-sitosterol (7.08%) were identified in mutant plantlet. HCA showed diversity between wild-type and mutant plantlets, which highest content of DDMP and stigmasterol and lowest of hydroxymethylfurfural of mutant plantlet.

Performance of Putative Mutants and Genetic Parameters of Plectranthus amboinicus (L.) through Mutation Induction With Colchicine Kunto Wibisono; Syarifah Iis Aisyah; Waras Nurcholis; Sri Suhesti
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 5 No 2 (2021): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Genetic enhancement in vegetatively propagated crops can be done through mutation induction. Colchicine-induced mutation is one of the methods that can be employed to increase plant genetic diversity. This study aimed to determine the effect of colchicine on the performance and genetic parameters of MV3 generation of Plectranthus amboinicus (L.). This study was conducted at the Laboratory of Agricultural Seed Management, Plantation Research and Development Center, from June 2018 to June 2019. Nodes and shoots were used as explants. Mutation induction was performed using colchicine at concentrations of 0%, 0.02%, 0.04%, and 0.06%. Explant regeneration and subculture were done on MS0 medium. The number of plantlets yielded were 59 (0% concentration of colchicine), 60 (0.02%), 81 (0.04%), and 80 plantlets (0.06%), respectively. Results indicated that colchicine-induced mutation in an in vitro culture was able to generate high genetic diversity in both quantitative and qualitative characters of the plantlets. At the concentration of 0.04%, colchicine produced the highest frequency of putative mutants (28.4%). Genetic parameters in MV3 generation of P. amboinicus plantlets showed that five quantitative characters, i.e. plantlet height, number of leaves, number of shoots, leaf length, and number of roots had high heritability values at a concentration around the LC50 value (0.0275%).

Co-Authors , Krisantini Abdul Hakim Agus Purwito Arum Sholikhah ASEP SAEFUDDIN Awang Maharijaya Aziz, Sandra A Azri Kusuma Dewi Azzahra, Elmi Irmayanti Bagas Akmala Putra Budi Marwoto Dewa Ngurah Suprapta Dewi Sukma Diny Dinarti Endang Gati Lestari Endang Gati Lestari Eny Rolenti Togatorop Fahmi Muhammad Cokrosudibyo Hafizah, Rumaisha Afifatul HAJRIAL ASWIDINNOOR Hasna Dama Hayati, Dhieni Helfi Eka Saputra Helfi Eka Saputra Ika Roostika Joko Ridho Witono Kartiman, Roni Kartiman, Roni Kunto Wibisono Kunto Wibisono Kunto Wibisono Kurniati, Ridho Latifah K Darusman Mariana Susilowati, Mariana Marthin, Yodi Marwoto, Budi Masna Maya SINTA Monica Christina Natalia Muhamad Rizal Martua Damanik Muhamad Syukur Muhammad Ridha Alfarabi Istiqlal Muhammad Riyo Agung Kurnia Muhammad Syukur Mulyana, Erik Muthi'ah Khairun Nisa Nabilah, Noorfakhriyah Ahsanti Natalia, Monica Christina Nella Angelina Simanjuntak Ni Made Armini Wiendi Niken Kendarini Nurul Komala Qisthi Kustia Rahman RAGAPADMI PURNAMANINGSIH Rahman, Qisthi Kustia RAHMI YUNIANTI Rapi, Muh. Nur Alif Rara Annisaur Rosyidah Ritonga, Arya Widura Riyan Alifbi Putera Irsal Rohim Firdaus ROHIM FIRDAUS Roni Kartiman Roni Kartiman Rosa Yunita Rosa Yunita Rusmiyati, Henny S Sudarsono Sari, Dia Novita Sarsidi Sastrosumarjo Sembiring, Rinawati Setiawan, Farensa Ikman Dedi Siti Hartati Yusida Saragih Sobir Sobir Sobir Sobir Sobir Sobir Sobir Sobir, dan Soeranto Human Sri Suhesti Sri Suhesti Sri Suhesti Sri Wahyuni Sukma, Dewi Sulassih, . Supijatno Suryawati, nFN Tamami, Djoko Theresia Dame Angel Lie Manurung Trikoesoemaningtyas Waras Nurcholis Wijaya Murti Indriatama Wijaya Murti Indriatama Winarni, Utin Wulan Septiningtyas Kurniajati yahidah Rosyidah Anshori Yasmin, Zahra Fadhlia Yoshua Shandy Yudha Yukarie Ayu Wulandari Yukarie Ayu Wulandari Yustia Yulianti Zahra Fadhlia Yasmin

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