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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Vegetalika Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmiah Aplikasi Isotop Radiasi Jurnal Hortikultura AGRIVITA, Journal of Agricultural Science Jurnal Agro Jurnal AGROSAINS dan TEKNOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biologi Tropis Journal of Tropical Crop Science AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Molekul: Jurnal Ilmiah Kimia Menara Perkebunan Jurnal Farmamedika (Pharmamedica Journal) Akta Agrosia Agrotech Journal

Syarifah Iis Aisyah

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian BogorJl. Meranti, Kampus IPB Darmaga, Bogor, 16680, Indonesia

Author-ID : 223003

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Respon empat varietas bawang putih (Allium sativum L.) lokal Indonesia terhadap media induksi dan proliferasi kalus embriogenik Hafizah, Rumaisha Afifatul; Sobir, Sobir; Aisyah, Syarifah Iis; Tamami, Djoko; Roostika, Ika
Jurnal AGRO Vol. 11 No. 2 (2024)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/37229

Establishing a regeneration media of Indonesian local garlic is necessary for several purposes, including plant breeding and large-scale propagation. This study was aimed to evaluate media formulation on callus induction and proliferation of four local garlic varieties (Geol, Lumbu Hijau, Lumbu Kuning, and Lumbu Putih) using root cuttings as the explants. MS media supplemented with different concentration of picloram (4 and 6 mg L-1) without and in combination with glutamine (100 mg L-1) alone and casein hydrolysate (3 g L-1) were evaluated. The results showed that the responses of induction and proliferation of embryogenic callus were genotype-dependent because there was no significant interaction between varieties and media formulations. Still, the varieties had a significant interaction with the observed variables. The fastest initiation time of callus induction was obtained from Lumbu Putih, less than 2 weeks after culture. Geol showed the highest percentage of callus formation and fresh weight of callus, 59% and 0,92 g respectively. There were three different types of the callus: (1) friable, glossy, clear white, (2) friable, glossy, transparent yellow, and (3) semi compact, glossy, yellowish to milky white. ABSTRAK Pemantapan media regenerasi bawang putih lokal Indonesia penting dilakukan untuk berbagai tujuan, termasuk pemuliaan tanaman dan perbanyakan skala besar. Penelitian ini bertujuan untuk mengetahui respon in vitro empat varietas bawang putih lokal (Geol, Lumbu Hijau, Lumbu Kuning, dan Lumbu Putih) terhadap komposisi media induksi dan proliferasi kalus dengan menggunakan akar sebagai eksplan. Komposisi media yang diujikan meliputi media dasar MS yang mengandung pikloram (4 dan 6 mg L-1), baik tanpa atau dengan penambahan glutamin (100 mg L-1) dan kasein hidrolisat (3 g L-1). Hasil penelitian menunjukkan respon induksi dan proliferasi kalus embriogenik bersifat genotype dependent, sebab tidak terdapat interaksi yang nyata antara faktor varietas dan formulasi media, namun faktor varietas berpengaruh nyata terhadap variabel amatan. Waktu inisiasi kalus tercepat diperoleh dari Lumbu Putih, yaitu kurang dari 2 minggu setelah kultur. Varietas Geol memiliki persentase pembentukan kalus dan bobot segar kalus tertinggi, berturut-turut sebesar 59% dan 0,92 g. Terdapat tiga tipe kalus yang terbentuk, yaitu (1) remah, mengkilap, putih bening, (2) remah, mengkilap, bening kekuningan, dan (3) kompak, mengkilap, kekuningan-putih susu.

Pertumbuhan dan Produksi Bunga Marigold (Tagetes erecta L.) pada Berbagai Frekuensi Pinching dan Jenis Pupuk: Growth and Flower Yield of African Marigold (Tagetes erecta L.) under Different Pinching Frequencies and Fertilizer Types Dewi Sukma; Syarifah Iis Aisyah; Muhamad Syukur; Dewa Ngurah Suprapta; Muthi'ah Khairun Nisa
Jurnal Hortikultura Indonesia (JHI) Vol. 14 No. 3 (2023): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.14.3.141-148

Pengaruh frekuensi pinching dan jenis pupuk terhadap pertumbuhan dan produksi T. erecta varietas (var.) Sudamala Oranye 1 perlu diketahui karena pelepasan varietas umumnya diiringi sistem budidaya yang sesuai dengan karakteristik tanaman. Penelitian dilaksanakan di Kebun Percobaan CV. Benih Dramaga, Desa Sinar Sari, Kecamatan Dramaga, Kabupaten Bogor, Jawa Barat pada bulan Maret hingga Juli 2023. Percobaan dilakukan pada T. erecta var. Sudamala Oranye 1 dengan bahan tanam setek menggunakan Rancangan Kelompok Lengkap Teracak (RKLT) faktorial dengan frekuensi pinching sebanyak 3 taraf, yaitu setiap 2 hari (F1), 4 hari (F2) dan 6 hari (F3) serta jenis pupuk sebanyak 2 taraf yaitu AB Mix 1000 ppm (J1) dan NPK 16-16-16 5000 + Gandasil 2000 (J2) sebagai faktor. Hasil penelitian menunjukkan frekuensi pinching berpengaruh nyata terhadap bobot kuntum serta sangat nyata terhadap diameter kuntum, sedangkan jenis pupuk berpengaruh nyata terhadap persentase tanaman terserang layu bakteri dan tanaman hidup, serta sangat nyata terhadap masa panen Sudamala Oranye 1 Pengamatan secara kualitatif pada kedua varietas menunjukkan adanya perbedaan warna daun dan mahkota bunga akibat jenis pupuk. Pinching setiap 2 hari memiliki komponen hasil tertinggi pada T. erecta varietas Sudamala Oranye 1 walaupun tidak berbeda nyata dengan frekuensi lainnya. Pemberian pupuk AB Mix 1000 ppm menghasilkan produksi tertinggi pada T. erecta varietas Sudamala Oranye 1.Kata kunci: AB Mix, gandasil, NPK, pembuangan kuncup, setek

Uji Keunggulan Genotipe Bunga Matahari Hasil Pemuliaan Tanaman IPB dalam Rangka Pelepasan Varietas Theresia Dame Angel Lie Manurung; Aisyah, Syarifah Iis; Muhamad Syukur
Jurnal Hortikultura Indonesia (JHI) Vol. 14 No. 1 (2023): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.14.1.33-39

Ketersediaan varietas unggul bunga matahari (Helianthus annuus L.) masih sangat rendah di Indonesia. Penelitian ini bertujuan meneliti karakter unggul dua genotipe bunga matahari hasil pemuliaan dan mengamati karakter kuantitatif dan kualitatifnya. Rancangan percobaan yang digunakan adalah Rancangan Kelompok Lengkap Teracak (RKLT) dengan faktor tunggal yaitu genotipe tanaman yang terdiri atas dua genotipe yang diuji, yaitu genotipe Kanigara dan genotipe Helina dan dua genotipe pembanding yaitu genotipe IPB BM1 dan genotipe Texas Lemon. Hasil analisis ragam keempat genotipe bunga matahari menunjukkan bahwa genotipe berpengaruh sangat nyata pada seluruh karakter kuantitatif kecuali diameter bunga tabung pada bunga sekunder dan panjang biji. Berdasarkan hasil analisis, Genotipe Helina merupakan genotipe terbaik dalam penelitian ini dengan beberapa keunggulan yaitu memiliki umur berbunga dan umur panen yang nyata lebih cepat, jumlah bunga di cabang yang nyata lebih banyak dibandingkan genotipe pembanding IPB BM1 dan jumlah bunga pita yang nyata lebih banyak dibandingkan dengan kedua genotipe pembanding. Genotipe Helina memiliki ciri khas warna bunga pita kuning dan biji berwarna hitam tanpa strip. Genotipe uji lainnya yaitu genotipe Kanigara memiliki bobot 100 biji yang nyata lebih tinggi dibandingkan genotipe pembanding dan bijinya berwarna coklat dengan strip. Kata kunci : Helianthus annuus L, karakter kualitatif, karakter kuantitatif, varietas unggul

Adaptability of Mutant Genotypes of Artemisia (Artemisia annua L.) as Result Of Gamma Irradiation in Three Locations with Different Altitude Syukur, Muhamad; Lestari, Endang Gati; Purnamaningsih, Ragapadmi; Yunita, Rosa; Aisyah, Syarifah Iis; Firdaus, Rohim
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.76

The objective of this study was to identify the adaptability of twelve artemisia mutant genotypes, which were planted in three locations with different altitude, as a result of gamma irradiation. Randomized Complete Block Design (RCBD) was applied in this research with three replications as blocks. The genotypes 1B, 1C, 1D, 2, 3, 4, 5A, 6B, 7A, 8, 14, 15 and two control genotypes as parent genotype from seed and from in vitro were used. The genotypes were planted in three different locations such as Mount Putri, Cianjur (1450 m above sea level), Pacet, Cianjur (950 m above sea level) and Cicurug, Sukabumi (540 m above sea level). Based on the method of postdictive and predictive success, the model used was AMMI2 which was able to explain up to 100% of interaction-influenced variation. The genotypes which were found stabile and adaptive in these three locations were 1B, 1C, 1D, 6B and 15. Genotypes 3 and 7A were adaptive specifically in Pacet area, 5A was adaptive for Gunung Putri while genotype 4 was for Cicurug only.Keywords: AMMI, Artemisia annua, mutant genotype, adaptability

Peningkatan Keragaman Genetik Tanaman Monstera adansonii melalui Induksi Mutasi Kimia dengan Streptomycin Susilowati, Mariana; Aisyah, Syarifah Iis; Rapi, Muh. Nur Alif; Kartiman, Roni
Vegetalika Vol 13, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.81993

Monstera adansonii variegata merupakan salah satu jenis tanaman hias daun yang popular di masyarakat sejak pandemi Covid-19. Variegata adalah perbedaan warna dan corak daun dengan tanaman normal yang diperoleh dari mutasi. Mutasi pada M. adansonii dapat dilakukan dengan berbagai mutagen kimia salah satunya streptomycin. Penelitian ini bertujuan mengetahui konsentrasi dan lama perendaman streptomycin yang optimum untuk menghasilkan tanaman M. adansonii variegata. Penelitian ini dilaksanakan selama 4 bulan di Kebun Percobaan IPB Sukamantri menggunakan Rancangan Kelompok Lengkap Teracak (RKLT) faktorial. Hasil penelitian menunjukkan bahwa interaksi antara konsentrasi streptomycin sebesar 750 ppm dengan lama perendaman 48 jam menunjukkan hasil yang paling baik diantara kombinasi perlakuan lainnya dengan rata-rata persentase mutan putatif sebesar 73,33%. Hasil uji lanjut menggunakan kontras polynomial menunjukkan bahwa konsentrasi dan lama perendaman streptomycin paling optimum untuk menghasilkan M. adansonii variegata adalah konsentrasi 860 ppm dengan lama perendaman 81,64 jam. Perubahan warna dan pola daun pada mutan putatif ini akan meningkatkan nilai ekonomi. Sementara itu antibiotik streptomycin juga mampu menghambat pertumbuhan dan perkembangan M. adansonii seperti tinggi tanaman, diameter batang, jumlah daun, dan luas daun.

Induksi Mutasi pada Stek Pucuk Anyelir (Dianthus caryophyllus Linn.) melalui Iradiasi Sinar Gamma Aisyah, Syarifah Iis; Aswidinnoor, Hajrial; Saefuddin, Asep; Marwoto, Budi; Sastrosumarjo, Sarsidi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 1 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

It has been a common knowledge that LD50 is commonly used in estimating optimal doses of gamma irradiation in a breeding program. This research was aimed at observing radiosensitivity of five carnation's genotypes to gamma irradiation, to find the LD50 of carnation's cuttings, and to obtain solid mutants from five numbers of Carnation. For cuttings, carnation genotype number 10.8 was the most insensitive to gamma rays, whereas number 24.15 was the most sensitive one. LD50 of carnation's cuttings were obtained around 49 -72 gray. There were 19 mutants produced from this treatment. The desired mutans were mostly produced from the treated 24.1 genotype whereas the character mutans were mostly observed in MV2 generation. Irradiation treatment on genotype 24.1 produced most stabile mutans while the less was in genotype 24.14. The produced mutants were qualitatively different in colour and petal shape, and stabile till third generation. Key words: LD50, gamma irradiation, induced mutation, carnation.

Ketahanan 25 Genotipe Tomat (Solanum lycopersicum Mill.) terhadap Pecah Buah dan Korelasinya dengan Karakter-karakter Lain Wahyuni, Sri; Yunianti, Rahmi; Syukur, Muhamad; Witono, Joko Ridho; Aisyah, Syarifah Iis
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 3 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACTFruit cracking is one of the major constraints in tomato production in lowland areas. This reduces fruit quality and quantity both as freshmarket and processed fruits. The objective of this study was to determine the resistance of 25 tomato genotypes to fruit cracking, correlation to others characters and their genetic similarity. The experiment was conducted from April until August 2012 at Leuwikopo Field Station, Bogor Agricultural University. A randomized complete block design was used with three replications. Fruit crack index was used to evaluate tomato fruit crack resistance. Correlation and impacts of variables were analyzed by path analysis. The genetic similarity was analyzed by clustering analysis. Result of resistance evaluation showed that fruit crack index of 25 tomato genotypes ranged from 0.00 to 20.58. Fruit crack index was directly related to percentage of fruit crack weight per plant, percentage of number of fruit crack per plant and total soluble solids. Cluster analysis showed that the genotypes could be clustered into four groups.Keywords: clustering analysis, genetic diversity, path analysis

Pendugaan Daya Gabung dan Heritabilitas Komponen Hasil Tomat pada Persilangan Dialel Penuh Saputra, Helfi Eka; Syukur, Muhamad; Aisyah, Syarifah Iis
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 3 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

ABSTRACT Hybridization is one of plant breeding techniques to obtain high yielding varieties. Combining ability and heritability information are needed for evaluating hybrids’ performance. The objective of this research was to estimate the combining ability and heritability of tomato yield component traits in a full dialel crosses at lowland. The experiment was conducted at Leuwikopo Experimental Station IPB, Darmaga, Bogor from March until August 2013. The random model of Griffing method I was used to estimate general combining ability, specific combining ability and heritability. The experiment used a randomized complete block design (RCBD) with three replications. IPBT1, IPBT13 and IPBT84 had a good general combining ability (GCA) for weight per fruit, fruit size and weight of fruit per plant. IPBT30 and IPBT33 had a good general combining ability (GCA) for number of fruits per plant. IPBT13 × IPBT1 hybrid showed a high and positive specific combining ability (SCA) values for all characters, therefore it can be used for hybrid variety. The highest broad and narrow sense heritability was obtained for fruit size, meanwhile the lowest was obtained for weight of fruit per plant. Weight per fruit, fruit size and weight of fruit per plant characters were influenced by additive and dominant gene effects, whereas number of fruit per plant was influenced by additive gene effects. Keywords: additive, dominant, GCA, SCA, heritability

Sensitivitas dan Keragaan Tanaman Coleus sp. terhadap Mutasi Induksi Kimia Menggunakan Ethyl Methane Sulfonate (EMS) Aplikasi Cara Rendam dan Tetes Sari, Dia Novita; Aisyah, Syarifah Iis; Damanik, dan Muhammad Rizal Martua
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Variant of Coleus sp. in Indonesia is very low. Induced mutation by Ethyl Methane Sulphonate (EMS) is able to increase variant of Coleus sp.. EMS is a mutagen that is effective and efficient in causing mutations. In mutation breeding, the high variant usually occurs around LC50 (Lethal Concentration). The objective of this study were (1) to obtain the LC50 value and sensivity of coleus plants, and (2) to evaluate the response of plant growth and to obtain new variance of coleus used soak and drops by EMS application. The experiment was arranged in randomized complete block design with three replications in each coleus. The materials used two Coleus sp. shoot cuttings i.e., C. amboinicus Lour. and C. blumei the purple/green color and the red. The concentration of EMS consisted of 0.00, 0.50, 0.75, 1.00, and 1.25% with application soak method (100 minutes) and drops (3 drops pipette). The results showed that the LC50 value of C. amboinicus Lour. 5.86% (soak). The LC50 value of C. blumei the purple/green color was 0.69% (soak), 0.82% (drops) and the red color 0.29% (drops), 0.89% (drops). C. blumei (the purple/green and red) was higher sensitivity rather than C. amboinicus Lour. There was an interaction between treatments on C. amboinicus Lour. whereas C. blumei (purple/green and red) had no interaction among treatments. C. amboinicus Lour. produced one putative mutant and C. blumei purple/green produced four putative mutants based on the morphology.Keywords: Coleus amboinicus Lour., Coleus blumei, LC50, putative mutan

Induksi Mutasi Tanaman Leunca (Solanum nigrum L.) untuk Meningkatkan Keragaman Kandungan Tanin Saragih, Siti Hartati Yusida; Aisyah, Syarifah Iis; Sobir, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 1 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Leunca (Solanum nigrum L. ) is one of the indigenous vegetables in Indonesia. The fruit of this vegetable is green and has bitter taste because of its tannin content. The objectives of this study were to get lethal dose 50 (LD50), diversity and to obtain candidates of mutant plants with high and low tannin content in young fruit and ripe fruit in leunca. The research was conducted at Pasir Kuda experimental station, Bogor; National Nuclear Energy Agency (BATAN), Jakarta; and postharvest laboratory of the Center for Tropical Horticulture Studies (PKHT IPB), Bogor, from December 2016 to July 2017. Leunca accession of SN 20 and SN 29 were irradiated using doses of 0, 100, 150, 200, and 250 Gy of gamma ray. The LD50 values for SN 20 and SN 29 were 171.944 Gy and 190.949 Gy, respectively. The analysis of tannin content was performed based on permanganate method by titration using potassium permanganate. The results showed that the highest tannin content of green stage fruit was found in SN20D3.5 with 200 Gy dose and the lowest was found in SN29D1.5 with 100 Gy dose. The highest tannin content of ripe fruit was found in SN20D4.6 with dose of 250 Gy and the lowest was found in SN20D3.1 with dose of 200 Gy. Recommended tannin content for leunca is the low tannin content.Keywords: diversity, gamma ray, indigenous vegetable, irradiation, LD50

Co-Authors , Krisantini Abdul Hakim Agus Purwito Arum Sholikhah ASEP SAEFUDDIN Awang Maharijaya Aziz, Sandra A Azri Kusuma Dewi Azzahra, Elmi Irmayanti Bagas Akmala Putra Budi Marwoto Dewa Ngurah Suprapta Dewi Sukma Diny Dinarti Endang Gati Lestari Endang Gati Lestari Eny Rolenti Togatorop Fahmi Muhammad Cokrosudibyo Hafizah, Rumaisha Afifatul HAJRIAL ASWIDINNOOR Hasna Dama Hayati, Dhieni Helfi Eka Saputra Helfi Eka Saputra Ika Roostika Joko Ridho Witono Kartiman, Roni Kartiman, Roni Kunto Wibisono Kunto Wibisono Kunto Wibisono Kurniati, Ridho Latifah K Darusman Mariana Susilowati, Mariana Marthin, Yodi Marwoto, Budi Masna Maya SINTA Monica Christina Natalia Muhamad Rizal Martua Damanik Muhamad Syukur Muhammad Ridha Alfarabi Istiqlal Muhammad Riyo Agung Kurnia Muhammad Syukur Mulyana, Erik Muthi'ah Khairun Nisa Nabilah, Noorfakhriyah Ahsanti Natalia, Monica Christina Nella Angelina Simanjuntak Ni Made Armini Wiendi Niken Kendarini Nurul Komala Qisthi Kustia Rahman RAGAPADMI PURNAMANINGSIH Rahman, Qisthi Kustia RAHMI YUNIANTI Rapi, Muh. Nur Alif Rara Annisaur Rosyidah Ritonga, Arya Widura Riyan Alifbi Putera Irsal Rohim Firdaus ROHIM FIRDAUS Roni Kartiman Roni Kartiman Rosa Yunita Rosa Yunita Rusmiyati, Henny S Sudarsono Sari, Dia Novita Sarsidi Sastrosumarjo Sembiring, Rinawati Setiawan, Farensa Ikman Dedi Siti Hartati Yusida Saragih Sobir Sobir Sobir Sobir Sobir Sobir Sobir Sobir, dan Soeranto Human Sri Suhesti Sri Suhesti Sri Suhesti Sri Wahyuni Sukma, Dewi Sulassih, . Supijatno Suryawati, nFN Tamami, Djoko Theresia Dame Angel Lie Manurung Trikoesoemaningtyas Waras Nurcholis Wijaya Murti Indriatama Wijaya Murti Indriatama Winarni, Utin Wulan Septiningtyas Kurniajati yahidah Rosyidah Anshori Yasmin, Zahra Fadhlia Yoshua Shandy Yudha Yukarie Ayu Wulandari Yukarie Ayu Wulandari Yustia Yulianti Zahra Fadhlia Yasmin

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