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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) AGRIVITA, Journal of Agricultural Science AL-HAYAT: Journal of Biology and Applied Biology AJAD : Jurnal Pengabdian kepada Masyarakat Jurnal Pengabdian Nasional (JPN) Indonesia
Puspito, Agung Nugroho
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Law, Crime, Criminology & Criminal Justice Social Sciences Other

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Salt and Heat Stress Trigger Morpho-Physiological Changes, Antioxidant Enzyme and Secondary Metabolites Gene Expression in Rice (Oryza sativa L.) Ubaidillah, Mohammad; Rozzita, Noor; Mufadilah, Mitha Aprilia; Thamrin , Nurhaliza; Puspito, Agung Nugroho; Kim, Kyung Min
HAYATI Journal of Biosciences Vol. 31 No. 2 (2024): March 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.2.256-270

Abstract

Climate change significantly increases salt and heat stress in rice plants. This condition causes plants to activate antioxidant enzymes and produce secondary metabolites. This study aimed to determine the morpho-physiological changes and gene expression profiles of antioxidant enzymes and secondary metabolites. This study used a completely randomized design factorial. The first factor was local rice varieties (IR64, Silaun, and Cigeulis), and the second factor was stress treatments (control, NaCl 150 mM, 40°C, and NaCl 150 mM + 40°C). The results showed that multiple stress significantly affected the plant height, stem length, stem diameter, leaf area, root length, total main root, plant biomass, necrotic length, chlorophyll content, relative water content, and plant ROS production. Multiple stress could up-regulate the gene expression of antioxidant enzymes (Mn-SOD, Cu/Zn SOD, Cytosolic APX, OsAPX1, CAT, OsCATA, and GPOD) in rice after stress combination treatments and increase the secondary metabolites gene expression (P5CS and GABA-T) in all rice varieties. Still, the OsNOMT gene was only active in the Cigeulis variety.
The Study of Exogenous Auxin and Cytokinins in Embryogenesis and Fiber Genes Expression during In Vitro Regeneration of Cotton (Gossypium hirsutum L.) Mufadilah, Mitha Aprilia; Thamrin , Nurhaliza; Puspito, Agung Nugroho; Ubaidillah, Mohammad
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.517-529

Abstract

The efficiency of regeneration media, based on the morpho-cytochemical response and embryogenesis gene expression of widely cultivated cotton in Indonesia (Gossypium hirsutum L.), needs to be studied further. This study's objectives are to identify the effect of 2,4-D and IBA hormones on the morphology and induction of cotton callus; to determine the effect of regeneration media on the morphology, cytochemical, and gene expression of cotton plant during embryogenesis. This study was conducted by inducing cotton callus on MS0 medium, MS Basal + 0.1 mg/L 2,4-D and MS Basal + 0.1 mg/L IBA. The best callus induction results were regenerated on the regeneration media, namely M1 (MS Vitamin + 0.01 ppm 2,4-D + 0.3 ppm IBA + 0.5 ppm Kinetin), M2 (MS Vitamin + 0.01 ppm 2,4-D + 0.5 ppm IBA + 0.5 ppm Kinetin), M3 (MS Vitamin + 0.00 ppm 2,4-D + 0.3 ppm IBA + 0.5 ppm Kinetin), and M4 (MS Vitamin + 0, 00 ppm 2,4- D + 0.5 ppm IBA + 0.5 ppm Kinetin). The embryogenesis gene expression was observed. The best morphological results for callus induction were obtained on MS Basal + 0.1 mg/L 2,4-D medium with a compact and white callus, indicates high embryogenic and regeneration ability. The highest percentage of regenerated callus parameters was found in M2 media and further confirmed by cytochemical characterization. Embryogenesis genes expressed were GhSERK1, GhSERK2, LEC, GhWUS, and GhWOX11. Other fiber-related genes GhMYB25-like, GhHD-1, and F3H were also expressed during the embryogenesis.
Pengenalan Agropreneur melalui Budidaya Tanaman Hidroponik di SDN 02 Patrang, Jember Ubaidillah, Mohammad; Karyaningtyas, Setyowati; Suud, Hasbi mubarak; Kumala, Velliana Dhea; Misi, Dina Aulia; Novita, Septian Abelia; Maharani, Adinda Putri; Jayanti, Silvi Dwi; Suryalaksono, Kemal Beryl; Puspito, Agung Nugroho
AJAD : Jurnal Pengabdian kepada Masyarakat Vol. 4 No. 3 (2024): DECEMBER 2024
Publisher : Divisi Riset, Lembaga Mitra Solusi Teknologi Informasi (L-MSTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59431/ajad.v4i3.391

Abstract

This research aims to increase students awareness of the environment through hydroponic cultivation at SDN 2 Patrang Jember. This research uses qualitative methods with observation, interviews and document analysis. The research results show that hydroponic cultivation at SDN 2 Patrang Jember has succeeded in increasing students' environmental awareness and knowledge about agriculture. This research also shows that hydroponic cultivation can be an effective alternative in increasing environmental awareness, as well as contributing to the development of environmentally caring character in students. Thus, this research can be a reference for other schools in developing effective greening programs in increasing environmental awareness in students.
Morpho-Physiological Response of Indonesian Rice (Oryza sativa L.) Under Combination Heat and Drought Stress Hartatik, Sri; Wardani, Dewi Kusuma; Choirunnisa, Eviyanti; Avivi, Sholeh; Puspito, Agung Nugroho; Kim, Kyung Min; Ubaidillah, Mohammad
AGRIVITA Journal of Agricultural Science Vol 46, No 3 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i3.4238

Abstract

Heat stress and drought are combination of stresses that have the potential to occur simultaneously in extreme temperature changes. Rice is the main food crop in Indonesia and includes plants that are sensitive to stress. Indonesian local rice is believed to have high adaptability to Indonesian's geographical conditions, however there is lack of updated data to explain this information. This study attempts to determine the level of adaptability or resistance of Indonesian local rice to a combination of heat stress and drought. The results showed that the 24 Indonesian local rice accessions were divided into 3 resistance classes. The high resistance class is Sintha, the resistance classes are Gajah Mungkur, Kalimutu, Kapuas, Sukamandi, Seratus Malam, Cabacu, Bengawan Solo and Cisokan, as well as the medium resistance classes Jatiluhur, Batang Ombilin, Mahakam, Nona Bokra, Pucuk, Bengawan, Rojolele, IR64, Barumun, Cisadane, Memberamo, Cibodas, Gilirang, Fatmawati and Ciherang.
Pengenalan Pola Hidup Sehat dan Bersih pada Siswa Sekolah Dasar Pancoran 3 Bondowoso Guna Mengatasi Stunting pada Usia Anak Sekolah Dasar Puspito, Agung Nugroho; Ubaidillah, Mohammad; Suud, Hasbi Mubarak; Faiqah, Qazzalva Nidya; Kaylani, Gyzna Luzetta; Aliyah, Rachmah; Putri, Duety Nectarinia; Azzahra, Ajeng Putri
Jurnal Pengabdian Nasional (JPN) Indonesia Vol. 6 No. 1 (2025): Januari
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM) STMIK Indonesia Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35870/jpni.v6i1.1242

Abstract

Bondowoso is one of the 3 cities with the highest stunting rates in East Java. This social project aims to introduce a healthy and clean lifestyle to elementary school students in Pancoran 3, Bondowoso Regency. to overcome the stunting often experienced by elementary school children, especially adolescent girls. In addition, this project also aims to change student behavior by implementing a healthy and clean lifestyle in everyday life. The research method used is conventional, which involves presentations, discussions, and practices on healthy and clean lifestyles with participants in grades 5 and 4 and aged 11- 12 years. This activity was carried out 4 times during October 2024. The study results showed that almost all elementary school students in Pancoran 3 understand healthy and clean lifestyles well, but many still do not understand how to apply healthy and clean lifestyles in everyday life.
Pengembangan Generasi Sehat Melalui Edukasi dan Implementasi Program Sanitasi serta Pola Hidup Sehat di MI Annidham Jember Ubaidillah, Mohammad; Sukaesih, Nabilah Shintia; Nur, Sefi Anindia Tiyana; Putri, Aulia Meuthia; Putra, Iqbal Firlana; Saputra, Martha Alit; Ramadhani, Vian; Puspito, Agung Nugroho
AJAD : Jurnal Pengabdian kepada Masyarakat Vol. 5 No. 1 (2025): APRIL 2025
Publisher : Divisi Riset, Lembaga Mitra Solusi Teknologi Informasi (L-MSTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59431/ajad.v5i1.433

Abstract

This social project aims to enhance students' knowledge and awareness of the importance of environmental sanitation and healthy lifestyles. Additionally, it seeks to modify their behavior by implementing sanitation and healthy living practices in their daily routines, such as regular handwashing, cleaning the classroom, and disposing of waste properly. The community service was carried out at MI Annidham, Sumbersari District, Jember Regency, with a total of 44 students from grades 3 and 4. The method employed was a conventional interactive approach, involving presentations, practice sessions, Q&A, and discussions during the implementation. The results of the community service indicate a 60% increase in students' knowledge and understanding of sanitation, hygiene, and clean and healthy lifestyle practices, which were the main topics addressed in the service activities. Furthermore, students' participation in this activity demonstrated high enthusiasm and a strong eagerness to learn, positively impacting their knowledge and skills.
Menanamkan Semangat Entrepreneur Berbasis Pada Daur Ulang Limbah Plastik di SDN Panti 2 Jember Ubaidillah, Mohammad; Shafany, Astry; Wardan, Nuril Maulidiyah; Nailah, Nurun; Maulina, Presilia Eka; Hoiriyah, Alfiatin; Azmi, Fatihah Ulul; Prasetyaningrum, Bintang Dwi; Puspito, Agung Nugroho
AJAD : Jurnal Pengabdian kepada Masyarakat Vol. 5 No. 1 (2025): APRIL 2025
Publisher : Divisi Riset, Lembaga Mitra Solusi Teknologi Informasi (L-MSTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59431/ajad.v5i1.434

Abstract

This social project focuses on environmental conservation efforts through a waste recycling program aimed at SDN Panti 2 students. The main objective of this project is to increase students' awareness and understanding of the importance of keeping the environment clean and teach them practical ways to recycle waste. This program consists of a series of educational activities that begin with counseling about the types of waste, the negative impact of waste on the environment, as well as recycling techniques and benefits. After that, students are invited to participate in various creative activities using used goods. The methodology used in this project is with conventional methods, namely presentations, discussions, games, and practicums. The results of this project show a significant increase in students' knowledge and awareness of the importance of waste recycling. The success of this program cannot be separated from the full support of the school and also the contribution of SDN Panti 2 students. And this project is a form of introducing the concept of entrepreneurship to students from the results of used goods. The main purpose of this project is to increase the value of students' creativity in making useful goods from recycled materials and introduce the economic potential of recycled products. It is hoped that students can develop positive habits that have a long-term impact on environmental preservation and entrepreneurial skills.
Proyek Gemilang: Pembentukan Karakter melalui Pendekatan Literasi, Sains, dan Keterampilan di SD Curahdami 3 Azizah, Qurrotun; Zabita, Devara Fulavelya; Nandini, Salma; Saputra, Aris Yulio; Jazirah; Candra, Yoga Andika; Suud, Hasbi Mubarak; Puspito, Agung Nugroho
AJAD : Jurnal Pengabdian kepada Masyarakat Vol. 5 No. 1 (2025): APRIL 2025
Publisher : Divisi Riset, Lembaga Mitra Solusi Teknologi Informasi (L-MSTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59431/ajad.v5i1.436

Abstract

The Gemilang Project aims to improve the competency of students at SDN Curahdami 3, Bondowoso, through project-based education that develops cognitive, emotional and physical aspects. Basic education plays an important role in forming quality individuals by emphasizing life skills and creativity. This project will be implemented over four weeks in the 2023/2024 academic year, involving practical activities such as planting chilies and creative activities such as drama and dance. The results show an increase in students' knowledge, skills and creativity. The use of innovative and traditional learning media has succeeded in increasing students' interest in learning. In conclusion, the project-based approach is effective in developing students' holistic potential. Thanks are expressed to all parties who contributed to the success of this project.
Pengenalan Healthy Nutritious Food Knowledge dan Personal Hygiene di SD Negeri Gadingsari, Kecamatan Binakal, Kabupaten Bondowoso Puspito, Agung Nugroho; Ubaidillah, Mohammad; Widyawati, Arista; Nabyla, Cahyani Farhatin; Amami, Nurillah Adi Aurura; Herlambang, Yasina Alhadila Nazima
Jurnal Pengabdian Nasional (JPN) Indonesia Vol. 6 No. 2 (2025): Mei
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM) STMIK Indonesia Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63447/jpni.v6i2.1246

Abstract

Children's ability to eat healthy and nutritious food is closely related to education. Education is an inseparable process as an effort to grow and develop the potential in every human being. Especially in children, education plays an important role in forming a healthy diet and good hygiene behavior. However, in several studies in Indonesia, it is not uncommon to find that there is still a lack of knowledge about healthy and nutritious food and good hand washing among elementary school students which harms their health. As in SDN Gadingsari, Binakal District, there are still many elementary school students who have a low understanding of healthy and nutritious food. The author's purpose in carrying out this social project is to socialize, provide education and understanding directly to SDN Gadingsari students about the importance of eating healthy and nutritious food and good hygiene behavior through interactive learning such as showing videos as a result of eating nutritious healthy food, washing hands, providing material on the concept of the contents of my plate, the content in nutritious food such as carbohydrates, protein, fat, fiber, vitamins, and minerals, games to gymnastics together. The results showed that the students were very enthusiastic about learning about healthy and nutritious food. It is hoped that students can apply it in their daily lives.
Meningkatkan Nilai Pancasila terhadap Siswa-Siswi di SDN Jetis 3 untuk Menanamkan Jiwa Kewarganegaraan Rahmatullah, Faisal; Abdurrohman, Ahmad; Hizkia, Ryandhika Risfi; Ramadani, Sintia Dwi; Nafisa, Amalia Putri; Puspito, Agung Nugroho
Jurnal Pengabdian Nasional (JPN) Indonesia Vol. 6 No. 2 (2025): Mei
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM) STMIK Indonesia Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63447/jpni.v6i2.1455

Abstract

This study aims to improve the understanding and application of Pancasila values in 5th grade students at SDN Jetis 3, Bondowoso, as an effort to instill the spirit of citizenship from an early age. The background of the study is based on the importance of character and moral education through the internalization of Pancasila values, considering that there are still many students who only memorize without understanding the meaning and implementation in everyday life. This study uses qualitative methods with a social project approach, including observation, provision of material, discussion, pre-test and post-test, as well as evaluation through interviews with teachers and principals. The results of the activity showed an increase in students ' understanding of the meaning and application of Pancasila values, both in the school and family environment. Active and contextual cultivation of Pancasila values has proven effective in shaping the character, attitude, and behavior of students who are ethical and have a spirit of nationalism. This study recommends collaboration between schools and families in familiarizing the application of Pancasila values to form a young generation with noble character and able to face the challenges of globalization.
Co-Authors A. F, Ahmad Noor Ziyan Abdurrohman, Ahmad Adhitya Wardhono Adinda Putri Maharani Affiana, Alfina Nur Afifurrohman, Ahmad Fata Afila, Fransiska ahmad rizal Akham, Qornan Hafidhul Alifiona, Vivi Nurhidayatul Aliyah, Rachmah Amami, Nurillah Adi Aurura Amelia, Wirda Fransisca Amelyda Ananta, Radina Widya Andini, Azizah Dian Anggira, Desi Aprilia, Sofi Arifqi, Muhamad Ali Ibrahim Arinanta, Arya Arniyanti, Muslimah Ashilia, Indah Aulia, Isthifa Annisa Aurelya, Lalyta Az-zahro, Aulia Fatimah Azizah, Qurrotun Azmi, Fatihah Ulul Azzahra, Ajeng Putri Bayu Aprillianto Buqori, Dwi Mai Abdul Imam Cahyani, Ardhya Pramisti Regita Candra, Yoga Andika Choirunnisa, Eviyanti D. R, Ernanda Mutiara Dewari, Siti Komariyah Khansa Dewayani, Nyiur Rahma Dewi Kusuma Wardani Dewi, Ruli Kusuma Elnadia, Salsabillah Rahmah Erzaldi, M Dwiki Fadilah, Iftitah Arya Putri Faiqah, Qazzalva Nidya Faizyah, Nadia Nurdiyana Fatmawati, Yeni Fauziah, Rafika Febriani, Nawal Aprodita Febrianti, Anis Febriyanti, Dwi Aidil Ferdyansyah, Dandy Firdaus, Rayhan Wahyu Gontha, Chelsea Tiara Hapsari, Salu Silvi Hartita, Margaretha Putri Harun, Anindita Firma Aulia Hasanah, Lailiyah Maulidatul Herlambang, Yasina Alhadila Nazima Hidayat, Adrian Shevchenko Hizkia, Ryandhika Risfi Hoiriyah, Alfiatin I, Putri Maulida Ilyaza, Debrin Syafrila Najwa Imelda, Laela Vita Innabilla, Mirsya Rifah Aulia Intansari, Herlindha Angghelinna Devfi Isnainun, Ainaya Jayanti, Silvi Dwi Jazirah Karin, Sheila Aulia Ismi Karyaningtyas, Setyowati Kaylani, Gyzna Luzetta Kim, Kyung Min Kirana, Zakiyah Galuh Pusphita Candra Kristianawati, Fany Indah Kumala, Velliana Dhea Kumalasari, Nandya Alfina Kurniawan, Bintang Indra Laela, Intan Laili, Anneja Maghfirotul Lestari, Dhea Suci Lestari, Ria Nova Agustin Puji Maharany, Adelia Dwifa Marvintha, Kirana Ndaru Maulina, Presilia Eka Ma’arif, Samsul Misi, Dina Aulia Mubarakah, Lailatul Mufadilah, Mitha Aprilia Mutawakkil, Muhammad Afif N, Khavita Rahmania Nabila, Nailun Nabyla, Cahyani Farhatin Nadhifa, Gusti Ayu Fillaily Nafisa, Amalia Putri Nailah, Nurun Nandini, Salma Nariswari, Septiya Nasir, M Abd Nasir, M. Abd. Nawu, Maria Natalia Nayyiroh, Dini Anjum Milatin Ningsih, Arumiyati Nisah, Ulfatun Novita, Septian Abelia Nugroho, Alfin Nur Hasanah Nur, Sefi Anindia Tiyana NURUL HIDAYAH Oktavia Rahma Prakoso, Bhim Prasetyaningrum, Bintang Dwi Pratama, Muhammad Reyhan Iwan Puspitasari, Michele Stefany Nazwa Putra, Iqbal Firlana Putri, Aulia Meuthia Putri, Duety Nectarinia Putri, Izzah Kamelia Putri, Revita Salsabila Qori’ah, Ciplis Gema Rachma P. A, Shabrina Rafa, Muhammad Rahmadini, Intan Aulia Rahmanda, Rahadian Rahmatullah, Faisal Ramadani, Sintia Dwi Ramadhani, Nabila Fajrin Kurnia Ramadhani, Vian Rendratama, Alvian Riskiyanto, Mohammad Rohman, M Andik Fathur Rosanti, Erine Farah Rozzita, Noor Rusdi, Nabila Maulida Saindra, Rafi Dzulfiqar Faiz Salsabila, Adelia Syahara Salsabila, Ayu Saputra, Aris Yulio Saputra, Martha Alit Sari, Sasmita Sarifudin, Ahmat Sattya Arimurti Setiawan, Bagus Adi Shafaana, Raihana Aulia Shafany, Astry Sholeh Avivi Sifah, Isrina Sinulingga, Trisna Handayani BR Siswanto, Siti Mazzhidha Rhaumi Novia Sofwah, Ayaa Sri Hartatik Su'udi, Mukhamad Sukaesih, Nabilah Shintia Sulistyowati, Rici Maulidya Sultonia, Ivone Suryalaksono, Kemal Beryl Suud, Hasbi Mubarak Syahzanan, Fatimah Tazkiya, Anisa Thaka, Ichan Ryan Thamrin , Nurhaliza Ubaidillah, Mohammad Ubay, Muhammad Umami, Fajar Fairi Wahyu, Dahlia Tiara Trisnaning Wardan, Nuril Maulidiyah Wardani, Amanda Julia Wibowo, Kaisar Belmiro Khalfani Widyawati, Arista Wijayanti, Karmila Dwi Yoniksa K, Bintang Yusuf, Zahrah Mufidah Zabita, Devara Fulavelya Zahrosa, Dimas Bastara Zaphora, Shineya Zennita