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All Journal Ecotrophic, Journal of Environmental Science JURNAL KIMIA SAINS DAN APLIKASI Jurnal Berkala Epidemiologi Journal Of Vocational Health Studies Media Ilmu Kesehatan Jurnal Medik Veteriner Bio-Inoved : Jurnal Biologi-Inovasi Pendidikan Jurnal Widya Biologi Jurnal Sewaka Bhakti Indonesian Journal of Global Health research Jurnal Bali Membangun Bali Widya Kesehatan Journal of Pharmaceutical and Health Research Journal of Multidisciplinary Applied Natural Science Jurnal Sains dan Teknologi Jurnal Sains dan Kesehatan Journal of Medicine and Health Technology Indonesian Journal of Pharmacology and Therapy
Ni Luh Gede Sudaryati
Department Of Biology, Faculty Of Information Technology And Science, Hindu University Of Indonesia
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Toxicity of Tangiayu Incense Smoke as an Insecticide Against Aedes aegypti Mosquito Mortality I Made Dwi Mertha Adnyana; I Putu Sudiartawan; Ni Luh Gede Sudaryati
MEDIA ILMU KESEHATAN Vol 10 No 3 (2021): Media Ilmu Kesehatan
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/mik.v10i3.637

Abstract

Background: Natural substances such as beluntas leaves, lemongrass leaves, and wood powder packaged in Tangiayu incense can be used to control vectors.Objective: This study was to determine the toxicity of Tangiayu smoke insecticides on Aedes aegypti mosquito mortality.Methods: Experimentation with a completely randomized design was applied to eight hundred seventy-five mature Aedes aegypti mosquitoes, divided into seven treatment groups with five replications, each unit consisting of 25 mosquito tests (n = 25). The data was collected from Aedes aegypti mosquito mortality following exposure to Tangiayu incense smoke for 5, 10, 15, 20, and 25 minutes. The ANOVA, LSD, and Probit tests were used to examine mortality data. Results: According to the research, there was an increase in Aedes aegypti mosquito mortality following the exposure smoke Tangayu incense. The exposure duration that resulted in 50% mortality was 18 min 30 s (LT50=5.9011), which is classified as extremely hazardous. The probit test resulted in equation 5= 0.0582x + 5.627. The coefficient of determination (R2) is 0.9718 (97.18%) and the coefficient correlation (r) is 0.9858 (98.58%).Conclusion: Exposure to Tangiayu incense smoke on Aedes aegypti mosquitoes mortality is extremely hazardous and effectively used as insecticides.
PERTUMBUHAN BAKTERI Salmonella thypi DAPAT DIHAMBAT DENGAN AIR REBUSAN DAUN KEMANGI (Ocimum basilicum L.) TETAPI TIDAK DENGAN AIR PERASANNYA. Putu Alit Wardana; I Nyoman Arsana; Ni Luh Gede Sudaryati
JURNAL WIDYA BIOLOGI Vol 13 No 01 (2022): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kemangi (Ocimum basilicum L) telah dimanfaatkan sebagai bahan obat untuk mengatasi berbagai penyakit. Penelitian ini bertujuan untuk mengetahui daya hambat air rebusan dan perasan daun kemangi terhadap pertumbuhan bakteri Salmonella thypi. Penelitian menggunakan rancangan acak kelompok dengan 12 kelompok perlakuan yaitu perasan daun kemangi konsentrasi 20%, 40%, 60%, 80%, 100%, dan rebusan daun kemangi dengan konsentrasi 20%, 40%, 60%, 80%, 100%, ditambah dengan satu kontrol positif (chloramphenicol) dan satu kontrol negatif (aquades). Rebusan daun kemangi disiapkan dengan merebus 50g daun pada suhu 90oC selama 15 menit kemudian disaring, sedangkan perasan disiapkan dengan menghancurkan 50g daun dan airnya disaring. Daya hambat ditentukan dengan medote cakram disk pada media Muller-Hilton Agar yang telah diinokulasi S. thypi. Hasil penelitian menunjukkan rebusan daun kemangi pada konsentrasi 20%, 40%, 60%, 80%, 100%, memiliki zona hambat terhadap pertumbuhan bakteri S. thypi, berturut-turut sebasar 0 mm, 8.67± 0.881 mm, 11.67±0.333 mm, 12±0.577 mm, dan 13.33±0.333 mm, sedangkan air perasan tidak memiliki zona hambat. Kesimpulan, pertumbuhan bakteri S. thypi dapat dihambat dengan rebusan daun kemangi tetapi tidak dengan air perasan.
Diagnostik Klinis Infeksi SARS-CoV-2 dengan Metode Apusan Ni Luh Gede Sudaryati; Ni Luh Nyoman Vivi Tri Handayani; I Putu Sudiartawan
INSOLOGI: Jurnal Sains dan Teknologi Vol. 1 No. 5 (2022): Oktober 2022
Publisher : Yayasan Literasi Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55123/insologi.v1i5.979

Abstract

COVID-19 has become a pandemic and has caused concern for people worldwide. The rapid spread of the disease requires health care providers and health workers to be able to diagnose precisely, quickly, and accurately to speed up the stages of treatment. Reviews and procedures for implementing clinical diagnostics of SARS-CoV-2 have not been widely reported. This study aimed to analyze the differences in diagnostic procedures for COVID-19 virus infection using the smear method at the Graha Medika Clinic. Observational descriptive research was conducted in July 2022. The research took place at the Graha Medika Clinic by screening patients who took the COVID-19 examination using the smear method (antigen swab, PCR swab, and rapid antigen). The study results obtained that one (10%) out of ten people infected with COVID-19 were on the antigen swab examination. There are differences in clinical diagnostics of COVID-19 infection using the smear method, namely in the antigen swab, the test time tends to be fast compared to PCR but is somewhat less accurate; rapid antigen only detects viruses that have taken place acutely with low accuracy while PCR is used as an accurate diagnostic by identifying proteins and viral glycans molecularly, has excellent accuracy but requires experts and a long time. The clinical diagnosis of COVID-19 infection using the smear method is currently adequate. The recommended examination method for the COVID-19 examination is carried out in stages, starting from rapid antigen, antigen swab, and PCR swab. PCR swab examination is the gold standard for detecting viral genetic material.
INFEKSI SALURAN KEMIH PADA PASIEN DIABETES MELITUS Ni Nyoman Triyani; I Nyoman Arsana; Ni uh Gede Sudaryati
JURNAL WIDYA BIOLOGI Vol 13 No 02 (2023): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32795/widyabiologi.v13i02.3565

Abstract

Diabetes melitus menimbulkan berbagai komplikasi lain seperti Infeksi Saluran Kemih. Penelitian ini bertujuan untuk mengkaji infeksi saluran kemih pada penderita diabetes mellitus. Penelitian menggunakan metode cross-sectional. Sebanyak 43 orang pasien terlibat dalam penelitian ini. Sampel urine pasien diperiksa secara mikroskopis, hasil positif mikroskopis kemudian dilanjutkan dengan metode kultur agar. Hasil penelitian menunjukkan sebanyak 11 orang dinyatakan positif infeksi saluran kemih. Pasien dengan hasil positif sebagian besar berjenis kelamin perempuan, terjadi pada usia diatas 60 tahun, serta lebih banyak terjadi pada penderita dengan diabetes lebih dari 10 tahun. Kesimpulan, perempuan dengan diabetes melitus lebih rentan terkena infeksi saluran kemih dibandingkan dengan laki-laki. Usia, lama menderita diabetes, dan Indeks Massa Tubuh juga menunjukkan ada keterkaitan erat dengan Infeksi Saluran Kemih.
Development of Organic Medicine Plants as Solutions for Improving The Economy and Health in Penatih Village During COVID-19 Anak Agung Putu Agung Mediastari; Ni Luh Gede Sudaryati; Anak Agung Komang Suardana
Journal of Pharmaceutical and Health Research Vol 3 No 3 (2022): October 2022
Publisher : Forum Kerjasama Pendidikan Tinggi (FKPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47065/jharma.v3i3.2805

Abstract

The COVID-19 pandemic has caused many social problems, especially the decline in the quality of health due to the lack of people's sources of income. The cultivation of organic medicinal plants in the community is a solution to improve the degree of health and help the community's economic recovery. The community does not have the understanding and ability to cultivate organic medicinal plants and use them as medicine and a source of income. This program aims to promote understanding, capability, and implementation of methods for cultivating organic medicinal plants, post-harvest processing, and their use as medicine and generate household-level economic value. The mentoring process lasted for six months in Penatih Village. Assistance is carried out by providing understanding, knowledge, and practice in developing organic medicinal plants through workshops and assisting the governance of land use, cultivation results, and their use for medicine and economic value. Evaluation of the community's knowledge, understanding, and skills is carried out through post-program interviews. The assistance results show that the community feels the impact of the organic medicinal plant seeds provided. Planting organic herbal medical plants and generating herbal medicinal raw materials with high economic value, such as empon- empon (rhizomes), to raise people's family income increases their knowledge, understanding, and abilities of land management governance. This activity is meant to be long-lasting so that the Organic Herbal Medicine Tourism Village can be built in Penatih Village and help the area's economy.
Aktivitas Antibakteri Loloh (Obat Tradisional Bali) Air Perasan dan Air Rebusan Daun Sirih terhadap Bakteri Streptococcus pyogenes Penyebab Radang Tenggorokan I Made Sumarya; I Wayan Suarda; Ni Luh Gede Sudaryati
Jurnal Kimia Sains dan Aplikasi Vol 22, No 5 (2019): Volume 22 Issue 5 Year 2019
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2442.967 KB) | DOI: 10.14710/jksa.22.5.173-178

Abstract

Sore throat is a respiratory infection caused by a bacterial infection of Streptococcus pyogenes. Betel leaf is used for loloh (traditional medicine) which has antimicrobial activity. The purpose of this study was to determine the content of active compounds and the antibacterial activity of the boiled water and juice of betel leaf against Streptococcus pyogenes. Explorative research using GC-MS method was used to identify and determine the abundance (content) of active compounds from loloh while the experimental research with the Randomized Post Test Only Control Group Design was carried out by treating the samples in four groups those are the negative control group with distilled water, the positive control group with 30 μg vancomycin, the treatment group with boiled water of betel leaf and betel leaf juice water to prove the antibacterial activity of loloh. The antibacterial activity data of the study results were analyzed statistically nonparametric through the Kruskal-Wallis test. The results showed that the betel leaf boiled water contained 0.472% hydroxicavicol active compound and had an average antibacterial activity of 6.50±0.224 mm against Streptococcus pyogenes bacteria. Whilst the juice of betel leaves did not contain active compounds and had no antibacterial activity.
BLOOD SMEAR PROFILE OF PATIENTS WITH DENGUE HEMORRHAGIC FEVER IN BALI ROYAL HOSPITAL Adnyana, I Made Dwi Mertha; Sudaryati, Ni Luh Gede; Suardana, A.A Komang
Journal of Vocational Health Studies Vol. 5 No. 1 (2021): July 2021 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V5.I1.2021.39-46

Abstract

Background: Each year, dengue hemorrhagic fever has grown. Clinical hematological examinations and blood preparations are used to confirm the diagnosis. Purpose: To detect, assess and characterize dengue hemorrhagic fever blood smear at Bali Royal Hospital. Method: Cross-sectional descriptive observational study-was conducted in April at Bali Royal Hospital on 37 patients with dengue hemorrhagic fever, clinical hematological tests and the manufacture of blood preparations identified using inclusion and exclusion criteria. Platelet counts, hemoglobin levels, hematocrit values, leukocyte counts, clinic degrees, gender and age, and blue plasma lymphocytes, are all used in hematological analyzed by univariate, ANOVA and BNT tests to analyze data. Result: Patients with dengue hemorrhagic fever are mostly toddlers and children (35.13%), women (54.0%), and dengue fever degree I (64.86%) with positive blue plasma lymphocytes (51.36%). Platelet counts, hemoglobin levels, hematocrit values, and leukocyte counts are significantly changed on days 3, 6, and 9, with probability values (p=0.000) less than p=0.01. The findings of the BNT test indicate that the number of platelets and haemoglobin on various days is significantly different (p<0.01), but the hematocrit value and leukocyte calculation are not significantly different (p>0.01). Conclusion: Clinical hematological tests and blood preparations reveal a blood component anomaly in Dengue Haemorragic Fever (DHF) patients at Bali Royal Hospital on days 3, 6, and 9.
EVALUATION OF REFILLABLE DRINKING WATER QUALITY BASED ON MPN COLIFORM AND ESCHERICHIA COLI IN SESETAN VILLAGE, SOUTH DENPASAR, BALI Wyasena, Pande Nyoman Tri Saguna; Sudaryati, Ni Luh Gede; Sudiartawan, I Putu; Adnyana, I Made Dwi Mertha
Journal of Vocational Health Studies Vol. 6 No. 2 (2022): November 2022 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V6.I2.2022.93-101

Abstract

Background: Refilled drinking water given by depots that currently have a permit for operation can be used to meet the community's drinking water demands. However, the quality of refilled drinking water has been tainted with pathogens that can cause health problems. Purpose: To assess the quality of replenished drinking water in Sesetan Village using Most Probable Number (MPN) Coliform and Escherichia coli as indicators. Method: A qualitative approach to descriptive observational research. The Ministry of Health standard 492/Menkes/PER/IV/2010 is used to assess drinking water quality. In this investigation, ten samples were used. Sampling was conducted in Sesetan Village, South Denpasar, Bali. Result: MPN Coliform was found in four samples: 96 MPN/100 mL (X1), 38 MPN/100 mL (X7), 15 MPN/100 mL (X8), and 5 MPN/100 mL (X10). Meanwhile, all of the samples tested negative for Escherichia coli. Conclusion: Based on drinking water quality criteria, 4 (40%) refilled drinking water depots (DAMIU) did not meet quality requirements (Ministry of Health Regulation number 492/Menkes/PER/2010).
Honey Purity Analysis of Trigona sp. species in Royal Honey Sakah, Bali Ni Wayan Susilawati; Ni Luh Gede Sudaryati; I Putu Sudiartawan
Jurnal Sains dan Kesehatan Vol. 5 No. 3 (2023): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v5i3.1550

Abstract

Honey is a sweet liquid produced by bees and derived from the nectar of flowers. The increasing circulation of fake honey has alarmed the public. An analysis of the purity of honey still needs to be reported. This study aimed to analyze the purity of honey from Trigona sp. at Royal Honey Sakah. The qualitative design was used during the study in May 2022 at Royal Honey Sakah. Three honey samples from different bees were analyzed to determine how much water, sugar, and viscosity they had. Our findings show that the purity of honey depends on moisture content, sugar content, and viscosity and that these three parameters depend on the quality of nectar produced from flower plants around the place of cultivation. The purity level in sugar, water, and viscosity content in three types of honey, including Apis dorsata honey, wild forest honey (Tetragonula biroi), and tenganan local honey (Apis sp.), all meet the quality requirements of honey according to SNI 8664: 2018. Therefore, the higher the sugar concentration, the less water there is in the honey, and the higher the viscosity, the more pure or authentic the honey is. People who will consume honey are expected to be able to identify the quality of honey in terms of its viscous structure, no bubbles resembling heated sugar, and a dark brown or concentrated color. Honey is declared pure if there is no further processing other than filtering from the source.
Adaptation of African Swine Virus in Non-Swine Cell Lines: A Preliminary Study for Vaccine Candidate Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, I Ketut; Apsari, Ida Ayu Pasti; Sari, Tri Komala; Handayani, Ni Made; Widayantari, Anak Agung Ayu Sauca Sunia; Suardana, Anak Agung Komang; Sumarya, I Made; Arsana, I Nyoman; Sudiartawan, I Putu; Wahyudi, I Wayan; Juliasih, Ni Ketut Ayu; Sudaryati, Ni Luh Gede; Damriyasa, I Made
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.114-123

Abstract

African Swine Fever (ASF) is among the most detrimental infectious viral diseases in pigs causing approximately 100% mortality. The disease was first reported about 83 years ago in Africa before spreading to Europe in 1957 and Asia in 2010. An adequate vaccine generally containing live attenuated virus isolates prepared in swine macrophages to control the disease is currently unavailable. Therefore, this study aimed to use murine neuroblastoma (N2a) cells, non-swine cell lines, to adapt African swine fever virus (ASFV) isolates for vaccine preparation. ASFV isolate called BL21 obtained from Bali and East Nusa Tenggara was previously propagated in swine macrophages. However, virus was currently adapted in the N2a cells to avoid unwanted issues associated with using swine macrophages, including microbial contamination, as well as technically laborious and ethical issues. The adapted BL21 was re-confirmed with quantitative polymerase chain reaction (q-PCR) and tested in vivo to examine the pathogenicity properties. The results showed that BL21 produced consistently and specifically positive q-PCR, killing experimental pigs with typical gross pathological changes of ASF. BL21 at a 10-3/mL dilution adapted in N2a cells showed similar antigenic properties causing the death of nearly 50% N2a cells in vitro and terminating all in vivo experimental pigs. In conclusion, the BL21 isolate reported in this study could be used as a vaccine candidate after more attenuation and particularly to determine a lethal dose of 50% (LD50) for future investigations.
Co-Authors A.A.A. Sauca Sunia Widyantari Adnyana , I Made Dwi Mertha Anak Agung Ayu Sauca Sunia Widyantari Anak Agung Komang Suardana Anak Agung Putu Agung Mediastari Armita, Pande Made Nova Astuti, Ni Made Widi da Cruz , Zito Viegas Dwinka Syafira Eljatin Farachatus Salwa Salsabila Azhari Gusti Ayu Putu Wahyu Purnama Dewi Handayani, Ni Made I Ketut Suada I Made Agus Gelgel Wirasuta I Made Damriyasa I Made Sumarya I Nyoman Arsana I Nyoman Arsana I Nyoman Arsana, I Nyoman I P. Sudiartawan I Putu Sudiarta I Putu Sudiartawan I W BUDIARSA SUYASA I Wayan Kasa I Wayan Martadi Santika I Wayan Masa Tenaya, I Wayan Masa I Wayan Suarda I Wayan Wahyudi, I Wayan I. A. R. Astiti Asih Ida Ayu Pasti Apsari Ida Bagus Putra Suta Ida Bagus Wiryanatha Kadek Karang Agustina N. W. Bogoriani Ni Kadek Warditiani Ni Ketut Ayu Juliasih Ni Ketut Ayu Juliasih Ni Ketut Ayu Juliasih Ni Luh Inten Mitadewi Wulandari Ni Luh Nyoman Vivi Tri Handayani Ni Made Listya Ari Ni Made Riris Divayani Aristyantari Ni Made Riris Divayani Aristyantari Ni Made Widiastuti Ni Nyoman Triyani Ni Wayan Ariska Yustina Ni Wayan Korniasih Ni Wayan Susilawati Noor, Thalia Anggrea Pande Made Nova Armita Putu Alit Wardana Putu Lakustini Cahyaningrum Putut Dewantha Jenar Raza, Syed Meesam Ronald Pratama Adiwinoto Suardana, A.A Komang Sudiartawan, I Putu Sumarya, I Made Suparmi Suparmi Suta, Ida Bagus Putra TRI KOMALA SARI Wayan Suryana Widayantari, Anak Agung Ayu Sauca Sunia Widyantari, Anak Agung Ayu Sauca Sunia Wyasena, Pande Nyoman Tri Saguna