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All Journal Media Kesehatan Masyarakat Indonesia Jurnal Ilmu Kedokteran (Journal of Medical Science) Majalah Kedokteran Bandung Jurnal Kesehatan Melayu JURNAL SIMETRIK Spektra: Jurnal Fisika dan Aplikasinya Pendas : Jurnah Ilmiah Pendidikan Dasar Indonesian Journal of Obstetrics & Gynecology Science Jurnal Surya Masyarakat Acta Biochimica Indonesiana Journal Of Widya Medika Junior Care : Jurnal Ilmiah Ilmu Kesehatan INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Surya Masyarakat Jurnal Pengabdian kepada Masyarakat Nusantara Unri Conference Series: Community Engagement Narra J Prosiding Konferensi Nasional PKM-CSR jabdimasunipem Jurnal Natur Indonesia Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Arfianti Arfianti
Departemen Biologi Kedokteran Fakultas Kedokteran Universitas Riau
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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IL-10 Promoter Polymorphism Distribution among HBsAg-Reactive and HBsAg-Nonreactive Blood Donors Rahmat Azhari Kemal; Arfianti Arfianti; Reni Oktora; Bebe Gani; Fauzia Andrini Djojosugito; Winarto Winarto
Majalah Kedokteran Bandung Vol 52, No 1 (2020)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (694.508 KB) | DOI: 10.15395/mkb.v52n1.1812

Abstract

Hepatitis B surface antigen (HBsAg) serves as a serological marker for Hepatitis B virus (HBV) infection. People with HBV asymptomatic infection might readily donate blood due to the lack of clinical manifestations. Host immunity contributes to susceptibility and progression of infection. A polymorphism in IL-10 gene promoter, rs1800896, might contribute to host immunity. This study was conducted on May 2019 – January 2020 in Faculty of Medicine, University of Riau on  70 blood samples from donors  in the Indonesian Red Cross Pekanbaru. Out of these samples, 35 were reactive for HBsAg and 35 donors were nonreactive. Genotyping of rs1800896 was conducted using Amplification Refractory Mutation System (ARMS-PCR). In total, The distribution of AA (74.3%), AG (24.3%), and GG (1.4%) genotypes revealed in this study seemed to be similar to genotype distribution among East and South-East Asian populations. While no significant difference was observed on age mean and gender distribution, a significant difference was identified in  genotype distribution between HBsAg status (p-value 0.028) with the percentage of AA genotype was higher among HBsAg-nonreactive donors (85.7%) compared to reactive donors (62.9%). More studies should be conducted to characterize HBsAg-reactive blood donors, including the donor characteristics and the viral genotypes. Such studies should contribute to hepatitis B management in Indonesia. Distribusi Polimorfisme Promoter IL-10 pada Donor Darah dengan HBsAg Reaktif dan Nonreaktif di Pekanbaru Hepatitis B surface antigen (HBsAg) merupakan penanda serologis infeksi virus hepatitis B (HBV). Individu dengan infeksi HBV asimptomatik dapat melakukan donasi darah karena tidak adanya gejala klinis. Imunitas inang berkontribusi pada kerentanan dan perkembangan infeksi. Polimorfisme pada promoter gen IL-10, rs1800896, dapat berkontribusi pada imunitas inang. Penelitian dilakukan pada bulan Mei 2019 – Januari 2020 di Fakultas Kedokteran Universitas Riau. Sampel diambil adalah 70 darah donor dari Palang Merah Indonesia Pekanbaru, 35 sampel reaktif HBsAg dan 35 sampel nonreaktif. Genotipe rs1800896 dilihat berdasarkan Amplification Refractory Mutation System (ARMS-PCR). Pada populasi studi ini, distribusi genotipe AA (74.3%), AG (24.3%), dan GG (1.4%) sesuai populasi Asia Timur dan Tenggara. Walaupun tidak terdapat perbedaan pada rerata umur dan distribusi jenis kelamin, penelitian ini menemukan perbedaan signifikan pada distribusi genotipe antar kelompok status HBsAg (p-value 0.028), yaitu persentase genotipe AA lebih tinggi pada kelompok donor dengan HBsAg nonreaktif (85.7%) dibandingkan kelompok reaktif (62.9%). Studi lebih lanjut perlu dilakukan untuk karakterisasi donor darah dengan HBsAg reaktif, termasuk gneotipe donor dan virus. Informasi tersebut diharapkan dapat bermanfaat pada manajemen hepatitis B di Indonesia.
Putative and pretreatment drug resistance mutations in reverse transcriptase gene among untreated chronic hepatitis B patients at Arifin Achmad Regional District Hospital, Riau, Indonesia Arfianti Arfianti; Fauzia Andrini Djojosugito; Maisaroh Maisaroh; Hendra Asputra; Dita Kartika Sari; Tubagus Odih Rhomdani Wahid
Acta Biochimica Indonesiana Vol. 5 No. 1 (2022): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.68

Abstract

Background: Mutations within the hepatitis B virus (HBV) reverse transcriptase (RT) gene have been associated with drug resistance against nucleos(t)ide analogs (NAs). Objective: This study aimed to identify mutations in the RT gene among patients with chronic hepatitis B (CHB) before receiving antiviral therapy and its relationship with the HBV genotypes. Methods: A total of 26 HBV DNA was extracted from the blood plasma of CHB patients. HBV RT gene was amplified and sequenced using the Sanger dideoxy sequencing method. The HBV genotype was determined through phylogenetic analysis using the Maximum Likelihood method. Results: The study subjects comprised 14 CHB patients without complications and 12 CHB patients with cirrhosis/hepatoma. CHB patients with cirrhosis/hepatoma were older than those without complications. The HBV genotypes comprised 15 (57.7%) genotype C and 11 (42.3%) genotype B. All treatment-naïve CHB patients did not demonstrate any classical NA resistance mutations within the RT gene. However, several putative and pretreatment resistance mutations, including F221Y, N238H, and V224I, were high frequency in more than 40% of study subjects. In addition, F221Y and N238H/Q mutations were frequently observed in genotype B, while V224 I was only found in patients infected with genotype C (p=0.000). Conclusions: There was no evidence of classical RT gene mutations associated with NA resistance in treatment-naïve patients with CHB. However, several putative and pretreatment mutations were identified as genotype-specific mutations and may contribute to antiviral resistance against NAs.
Peningkatan derajat kesehatan masyarakat Bagan Sinembah melalui pengelolaan sampah rumah tangga menjadi barang bernilai ekonomi Huriatul Masdar; Fifia Chandra; Arfianti Arfianti; Yulia Irvani Dewi
Unri Conference Series: Community Engagement Vol 1 (2019): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.1.158-162

Abstract

Household waste if not managed properly can cause various health problems. Good management of household waste can have economic value and improve the economy of community. Organic waste can be processed into compost that is useful for agricultural land while non-organic waste can be recycled into high-value items. This waste management problem also occurred in Bagan Sinembah Subdistrict, Rokan Hilir Regency. Through community service activities at University of Riau, socialization of organic and non-organic waste management, composting training and decoupage art applications on used goods in households were conducted. The level of success of this activity is considered good in quality, but the quantity of trainees is still lacking. Increasing the number of target communities needs to be done so that the waste management efforts at Bagan Sinembah become more optimal.
Peningkatan Kapasitas Kader Posyandu Desa Tambang Dalam Deteksi Stunting Pada Anak Arfianti Arfianti; Nurhasanah Nurhasanah; Suri Dwi Lesmana; Yuliati Yuliati; Erika Erika; Ariza Julia Paulina; Dedi Afandi
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 3 (2020): Peran Perguruan Tinggi dan Dunia Usaha Dalam Pemberdayaan Masyarakat Untuk Menyongsong
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.856 KB) | DOI: 10.37695/pkmcsr.v3i0.792

Abstract

Stunting merupakan kondisi malnutrisi kronis pada anak yang ditandai dengan tinggi badan anak berada di bawah 2 standar deviasi dibawah grafik pertumbuhan anak. Stunting menjadi masalah kesehatan yang serius terutama di negara-negara berkembang, termasuk di Indonesia. Kampar merupakan salah satu wilayah prioritas penanganan stunting di Provinsi Riau dengan 10 lokus stunting yang telah diidentifikasi. Desa Tambang Kecamatan Tambang merupakan desa di Kabupaten Kampar dengan prevalensi stunting cukup tinggi yaitu memiliki 42 balita. Tujuan dari kegiatan pengabdian ini adalah meningkatkan pengetahuan dan keterampilan kader Desa Tambang dalam melakukan penilaian status gizi anak balita. Pengabdian dilakukan pada bulan Juli 2020 di Kantor Camat Tambang. Metode pelatihan meliputi ceramah, tanya jawab, studi kasus dan praktek langsung pengukuran tinggi dan berat badan anak balita. Topik pelatihan meliputi stunting dan strategi pencegahan stunting di desa, penilaian status gizi anak balita serta kebutuhan gizi ibu hamil dan anak balita. Pelatihan diikuti oleh 25 kader posyandu yang ada di Desa Tambang. Hasil evaluasi kegiatan menggunakan kuesioner pre-test dan post-test menunjukkan terdapat peningkatan pengetahuan kader posyandu tentang stunting dan strategi pencegahannya.
Edukasi dan Pelatihan Tanaman Obat Keluarga di Kelurahan Sri Meranti, Pekanbaru Rahmat Azhari Kemal; Arfianti Arfianti; Ulfah Ulfah; Bayu Fajar Pratama
Jurnal Surya Masyarakat Vol 6, No 1 (2023): November 2023
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jsm.6.1.2023.11-15

Abstract

A family medicinal plant is a group of plants that have health benefits for family health. However, its utilization should be increased. During the COVID-19 pandemic, public interest in traditional herbal medicine increases. Therefore, it has to be balanced with increased knowledge of family medicinal plants as well as knowledge of good practices in the utilization and care of the plants. Education and training of family medicinal plants were conducted for representatives of Sri Meranti District, Pekanbaru City, Riau. Education on types of family medicinal plants and how to properly utilize them was delivered online via the Zoom Meeting platform. The education was followed by training on how to plant and care for the family's medicinal plants. Participants were also given seedlings of family medicinal plants for their yards. After the education, participants’ knowledge of how to utilize family medicinal plants increased. With the increased knowledge as well as the seedlings provided, people in the Sri Meranti district are expected to cultivate and utilize family medicinal plants properly.
Edukasi dan Pelatihan Tanaman Obat Keluarga di Kelurahan Sri Meranti, Pekanbaru Kemal, Rahmat Azhari; Arfianti, Arfianti; Ulfah, Ulfah; Pratama, Bayu Fajar
Jurnal Surya Masyarakat Vol 6, No 1 (2023): November 2023
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jsm.6.1.2023.11-15

Abstract

A family medicinal plant is a group of plants that have health benefits for family health. However, its utilization should be increased. During the COVID-19 pandemic, public interest in traditional herbal medicine increases. Therefore, it has to be balanced with increased knowledge of family medicinal plants as well as knowledge of good practices in the utilization and care of the plants. Education and training of family medicinal plants were conducted for representatives of Sri Meranti District, Pekanbaru City, Riau. Education on types of family medicinal plants and how to properly utilize them was delivered online via the Zoom Meeting platform. The education was followed by training on how to plant and care for the family's medicinal plants. Participants were also given seedlings of family medicinal plants for their yards. After the education, participants’ knowledge of how to utilize family medicinal plants increased. With the increased knowledge as well as the seedlings provided, people in the Sri Meranti district are expected to cultivate and utilize family medicinal plants properly.
Unveiling the impacts of metformin on hepatocellular carcinoma: A bioinformatic exploration in cell lines Soraya, Soraya; Arfianti, Arfianti; Adikusuma, Wirawan; Irham, Lalu M.; Hamidy, Muhammad Y.; Winarto, Winarto; Rangkuti, Ina F.; Darmawi, Darmawi
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.968

Abstract

The most common type of liver cancer is hepatocellular carcinoma (HCC), accounting for 75–85% of cases. Despite its associated side effects, sorafenib remains the standard treatment for HCC. Given the critical need to improve therapeutic efficacy while minimizing adverse effects, alternative drugs must be thoroughly investigated. Numerous studies indicate that combining sorafenib with metformin results in a more favorable treatment profile. The aim of this study was to employ bioinformatics methodologies to elucidate the molecular pathways and genetic underpinnings of metformin's efficacy in HCC treatment. Genes associated with metformin and its action against HCC (Huh-7 and HepG2 cells) were acquired from the NCBI-GEO data collection by utilizing pre-determined keywords. Subsequently, pathways implicated in metformin-mediated HCC treatment were analyzed through the Kyoto Encyclopedia of Genes and Genomes (KEGG). Our analysis revealed the involvement of multiple pathways, with metabolic pathways implicated in 80% of the total cases. Neurodegenerative pathways were involved in only around 60% of the total cases. These findings align with the multifaceted mechanisms of metformin’s action, encompassing adenosine monophosphate-activated protein kinase activation, apoptosis induction, insulin regulation, anti-inflammatory responses, and modulation of cell proliferation. This comprehensive investigation sheds light on the intricate molecular landscape underpinning metformin's therapeutic efficacy in HCC, thereby informing potential avenues for optimizing treatment strategies.
Prevalence of major INSTI and HIV-1 drug resistance mutations in pre- and antiretroviral-treated patients in Indonesia Djojosugito, Fauzia A.; Arfianti, Arfianti; Wisaksana, Rudi; Siregar, Fajri M.; Nasronudin, Nasronudin; Rachman, Brian E.; Khairunisa, Siti Q.; Indrati, Agnes R.
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.1022

Abstract

Indonesia has one of the highest HIV infection rates in Southeast Asia. The use of dolutegravir, an integrase strand transfer inhibitor (INSTI), as a first-line treatment underscores the need for detailed data on INSTI drug resistance mutations (DRMs). Currently, there is a lack of comprehensive data on DRMs INSTI and other HIV drug resistance in Indonesian patients, both pre- and post-treatment. The aim of this study was to identify the subtypes and drug resistance mutations of the protease, reverse transcriptase, and integrase genes in both treatment-naive and ARV-treated patients in Bandung, West Java, Indonesia. A cross-sectional study was conducted involving HIV-positive patients at Hasan Sadikin Hospital, Bandung, Indonesia, from September 2022 to January 2023. The patients were categorized into two groups: ARV-treated and pre-treatment patients. Peripheral blood mononuclear cells (PBMCs) were processed for DNA extraction, followed by amplification and sequencing of the pol gene to detect mutations and subtypes. The study found that the predominant subtype was CRF01_AE, accounting for 85.4% and 69% of pre-treatment and treated patients, respectively, followed by recombinant forms such as A1/CRF01_AE, CRF01_AE/CRF02_AG, subtype B, and other subtypes. Among ARV-treated/INSTI-naive patients, major INSTI DRMs R263K and Y143H were identified, while pre-treatment patients exhibited accessory integrase DRMs. The most common DRMs detected were non-nucleoside reverse transcriptase inhibitor (NNRTI) DRMs, with prevalences of 14.6% and 7% in pre-treatment and ARV-treated patients, respectively. In conclusion, CRF01_AE emerged as the predominant subtype in both pre-treatment and ARV-treated patients in Bandung, underscoring the necessity for ongoing surveillance of integrase DRMs, particularly given the presence of major INSTI DRMs in patients undergoing INSTI treatment.
Distribution and Clinical Significance of Nuclear Factor Erythroid 2-Related Factor 2 Gene Polymorphism in Chronic Hepatitis B: A Cross-Sectional Study Yuhendri, Vitriyanna Mutiara; Labecka, Magda; Ibrahim, Sundus; Arfianti, Arfianti
Jurnal Natur Indonesia Vol. 23 No. 1 (2025): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.23.1.10-15

Abstract

Chronic hepatitis B (CHB) infection is associated with serious complications, including liver cirrhosis and hepatocellular carcinoma (HCC). Nuclear factor erythroid 2-related factor 2 (NRF2) is a transcription factor that regulates the expression of antioxidant genes, helping protect cells and tissues from oxidative stress, a process involved in the pathogenesis of chronic hepatitis B (CHB). This study aimed to determine the distribution of NRF2 polymorphisms in CHB patients and their association with complications. The study included 68 CHB patients, with 33 having no complications and 35 with complications (Cirrhosis and HCC). Genotyping of the NRF2 polymorphisms, rs35652124 (A→G) and rs6721961 (C→A), was performed using confronting two-pair primers and polymerase chain reaction (PCR-CTPP). The serum levels of bilirubin, albumin, and alanine aminotransferase (ALT) were measured using commercial kits. The mean age of subjects was 45.34±1.32 years old on average. There was no significant difference in mean bilirubin and ALT levels between patients with and without CHB complications. However, patients without complications had significantly higher albumin levels than those with complications (4.0±0.8 vs. 3.37±0.7 g/dL; p<0.05). The most common genotypes for NRF2 rs35652124 were AG (51.85%), AA (40.74%), and GG (7.41%), while for NRF2 rs6721961, the were CA (51.47%), CC (45.59%), and AA (2.94%). The distribution of NRF2 genotypes did not differ significantly between CHB patients with and without complications (p>0.05). This study suggests that NRF2 gene polymorphisms may not contribute to the development of Cirrhosis and HCC in CHB. Further research with a larger sample size is needed to confirm these findings.
Vitamin D enhances migration but decreases gene expression of vascular endothelial growth factor and tumor necrosis factor-α in Wharton’s jelly mesenchymal stem cells Yuhendri, Vitriyanna Mutiara; Pratiwi, Nurfi; Paulina, Ariza Julia; Ismawati, Ismawati; Saputra, Nicko Pisceski Kusika; Arfianti, Arfianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6698

Abstract

Mesenchymal stem cells (MSCs) have gained significant interest for their potential application in repairing damaged tissues through immunomodulatory functions. Vitamin D has been shown to regulate both the innate and adaptive immune systems. Active forms of Vitamin D, such as 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) are known to influence the action of mesenchymal stem cells (MSCs) in several mechanisms, such as stimulating their proliferation and osteogenic differentiation, as well as tissue regeneration. This study aimed to investigate the effects of vitamin D supplementation on the biological properties and expression of cytokines and growth factor genes in MSCs isolated from the human umbilical cord (UC). MSCs were isolated from Wharton’s jelly (WJ-MSC) of UC, cultured, and supplemented with various concentrations of vitamin D3. The Cell Counting Kit-8 (CCK-8) assay was used to measure cell viability, and a scratch wound healing assay was conducted to evaluate the migration capacity of MSCs. The mRNA expression levels of vascular endothelial growth factor (VEGF), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 were quantified using reverse transcription-polymerase chain reaction (RT-PCR). This study showed that supplementation with 50 nM of vitamin D3 for 48 h significantly increased the viability and migratory capacity of MSCs. Furthermore, vitamin D supplementation significantly decreased the mRNA levels of TNF-α and VEGF but did not affect IL-6 gene expression compared to the control group. These findings suggest that vitamin D supplementation can enhance the biological characteristics and modulate the expression of key immunomodulatory factors in MSCs, potentially improving the effectiveness of MSC-based therapies.
Co-Authors Andi Zainal Ardini, Nadiah Yamanza Ariza Julia Paulina Armi, Wahyuli Bayu Fajar Pratama Bebe Gani Dani Rosdiana Darmawi Darmawi Dedi Afandi Deyana, Frilly Dita Kartika Sari Djojosugito, Fauzia A. Elda Nazriati Erika Erika Fauzan A. Sangadji Fauzia Andrini Fifia Chandra Fifia Chandra Hamidy, Muhammad Y. Hendra Asputra Hendri, Sherin Alifia Huriatul Masdar Ibrahim, Sundus Idawati Idawati Idawati Indrati, Agnes R. Irham, Lalu M. Ismawati ismawati Ismawati Ismawati Kesumah, Dewi Iramayana Sandra Khairunisa, Siti Q. Labecka, Magda Lazuardi Umar Maisaroh Maisaroh Muhammad Beni Septima Muhammad Nawir Muhammad Nawir Nababan, Heru Maranatha Nasronudin Nasronudin Nukman Moeloek Nurfi Pratiwi, Nurfi Nurul Humairah Arfiza Pernantah, Piki Setri Purnomo Soeharso Rachman, Brian E. Rahmat Azhari Kemal Rahmondia N. Setiadi Ramadhani, Nur'aina ID Rangkuti, Ina F. Reni Oktora Riefni Silara Dini Rina Amtarina Rita Endriani Rudi Wisaksana S, Donel S.Pd. M Kes I Ketut Sudiana . Saputra, Nicko Pisceski Kusika Sherin Alifia Hendri Simanjuntak, Arya Marganda Siregar, Fajri M. Soraya Soraya Suri Dwi Lesmana Suyanto Taihuttu, Felix TB Odih Rhomdani Wahid Tetty M Linda Tri Muhti Puja Kesuma Ulfah Ulfah Ulfah Ulfah Winarto Winarto Winarto Winarto Wirawan Adikusuma Yuhendri, Vitriyanna Mutiara Yulia Irvani Dewi Yulia Wardany Yuliati Yuliati Zarfiardy Aksa Fauzi