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All Journal Media Kesehatan Masyarakat Indonesia Jurnal Ilmu Kedokteran (Journal of Medical Science) Majalah Kedokteran Bandung Medical Journal of Indonesia Jurnal Kesehatan Melayu Spektra: Jurnal Fisika dan Aplikasinya Indonesian Journal of Obstetrics & Gynecology Science Jurnal Surya Masyarakat Acta Biochimica Indonesiana Journal Of Widya Medika Junior Care : Jurnal Ilmiah Ilmu Kesehatan INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Surya Masyarakat Unri Conference Series: Community Engagement Narra J Prosiding Konferensi Nasional PKM-CSR jabdimasunipem Jurnal Natur Indonesia Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Arfianti Arfianti
Departemen Biologi Kedokteran Fakultas Kedokteran Universitas Riau
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Prevalence of major INSTI and HIV-1 drug resistance mutations in pre- and antiretroviral-treated patients in Indonesia Djojosugito, Fauzia A.; Arfianti, Arfianti; Wisaksana, Rudi; Siregar, Fajri M.; Nasronudin, Nasronudin; Rachman, Brian E.; Khairunisa, Siti Q.; Indrati, Agnes R.
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.1022

Abstract

Indonesia has one of the highest HIV infection rates in Southeast Asia. The use of dolutegravir, an integrase strand transfer inhibitor (INSTI), as a first-line treatment underscores the need for detailed data on INSTI drug resistance mutations (DRMs). Currently, there is a lack of comprehensive data on DRMs INSTI and other HIV drug resistance in Indonesian patients, both pre- and post-treatment. The aim of this study was to identify the subtypes and drug resistance mutations of the protease, reverse transcriptase, and integrase genes in both treatment-naive and ARV-treated patients in Bandung, West Java, Indonesia. A cross-sectional study was conducted involving HIV-positive patients at Hasan Sadikin Hospital, Bandung, Indonesia, from September 2022 to January 2023. The patients were categorized into two groups: ARV-treated and pre-treatment patients. Peripheral blood mononuclear cells (PBMCs) were processed for DNA extraction, followed by amplification and sequencing of the pol gene to detect mutations and subtypes. The study found that the predominant subtype was CRF01_AE, accounting for 85.4% and 69% of pre-treatment and treated patients, respectively, followed by recombinant forms such as A1/CRF01_AE, CRF01_AE/CRF02_AG, subtype B, and other subtypes. Among ARV-treated/INSTI-naive patients, major INSTI DRMs R263K and Y143H were identified, while pre-treatment patients exhibited accessory integrase DRMs. The most common DRMs detected were non-nucleoside reverse transcriptase inhibitor (NNRTI) DRMs, with prevalences of 14.6% and 7% in pre-treatment and ARV-treated patients, respectively. In conclusion, CRF01_AE emerged as the predominant subtype in both pre-treatment and ARV-treated patients in Bandung, underscoring the necessity for ongoing surveillance of integrase DRMs, particularly given the presence of major INSTI DRMs in patients undergoing INSTI treatment.
Distribution and Clinical Significance of Nuclear Factor Erythroid 2-Related Factor 2 Gene Polymorphism in Chronic Hepatitis B: A Cross-Sectional Study Yuhendri, Vitriyanna Mutiara; Labecka, Magda; Ibrahim, Sundus; Arfianti, Arfianti
Jurnal Natur Indonesia Vol. 23 No. 1 (2025): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.23.1.10-15

Abstract

Chronic hepatitis B (CHB) infection is associated with serious complications, including liver cirrhosis and hepatocellular carcinoma (HCC). Nuclear factor erythroid 2-related factor 2 (NRF2) is a transcription factor that regulates the expression of antioxidant genes, helping protect cells and tissues from oxidative stress, a process involved in the pathogenesis of chronic hepatitis B (CHB). This study aimed to determine the distribution of NRF2 polymorphisms in CHB patients and their association with complications. The study included 68 CHB patients, with 33 having no complications and 35 with complications (Cirrhosis and HCC). Genotyping of the NRF2 polymorphisms, rs35652124 (A→G) and rs6721961 (C→A), was performed using confronting two-pair primers and polymerase chain reaction (PCR-CTPP). The serum levels of bilirubin, albumin, and alanine aminotransferase (ALT) were measured using commercial kits. The mean age of subjects was 45.34±1.32 years old on average. There was no significant difference in mean bilirubin and ALT levels between patients with and without CHB complications. However, patients without complications had significantly higher albumin levels than those with complications (4.0±0.8 vs. 3.37±0.7 g/dL; p<0.05). The most common genotypes for NRF2 rs35652124 were AG (51.85%), AA (40.74%), and GG (7.41%), while for NRF2 rs6721961, the were CA (51.47%), CC (45.59%), and AA (2.94%). The distribution of NRF2 genotypes did not differ significantly between CHB patients with and without complications (p>0.05). This study suggests that NRF2 gene polymorphisms may not contribute to the development of Cirrhosis and HCC in CHB. Further research with a larger sample size is needed to confirm these findings.
Vitamin D enhances migration but decreases gene expression of vascular endothelial growth factor and tumor necrosis factor-α in Wharton’s jelly mesenchymal stem cells Yuhendri, Vitriyanna Mutiara; Pratiwi, Nurfi; Paulina, Ariza Julia; Ismawati, Ismawati; Saputra, Nicko Pisceski Kusika; Arfianti, Arfianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6698

Abstract

Mesenchymal stem cells (MSCs) have gained significant interest for their potential application in repairing damaged tissues through immunomodulatory functions. Vitamin D has been shown to regulate both the innate and adaptive immune systems. Active forms of Vitamin D, such as 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) are known to influence the action of mesenchymal stem cells (MSCs) in several mechanisms, such as stimulating their proliferation and osteogenic differentiation, as well as tissue regeneration. This study aimed to investigate the effects of vitamin D supplementation on the biological properties and expression of cytokines and growth factor genes in MSCs isolated from the human umbilical cord (UC). MSCs were isolated from Wharton’s jelly (WJ-MSC) of UC, cultured, and supplemented with various concentrations of vitamin D3. The Cell Counting Kit-8 (CCK-8) assay was used to measure cell viability, and a scratch wound healing assay was conducted to evaluate the migration capacity of MSCs. The mRNA expression levels of vascular endothelial growth factor (VEGF), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 were quantified using reverse transcription-polymerase chain reaction (RT-PCR). This study showed that supplementation with 50 nM of vitamin D3 for 48 h significantly increased the viability and migratory capacity of MSCs. Furthermore, vitamin D supplementation significantly decreased the mRNA levels of TNF-α and VEGF but did not affect IL-6 gene expression compared to the control group. These findings suggest that vitamin D supplementation can enhance the biological characteristics and modulate the expression of key immunomodulatory factors in MSCs, potentially improving the effectiveness of MSC-based therapies.
Accuracy of Platelet Parameters (PC, MPV, and PDW) as A Predictor of The Severity of Preeclampsia Nababan, Heru Maranatha; S, Donel; Arfianti, Arfianti; Simanjuntak, Arya Marganda
Indonesian Journal of Obstetrics & Gynecology Science Volume 8 Nomor 2 July 2025
Publisher : Dep/SMF Obstetri & Ginekologi Fakultas Kedokteran Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/obgynia.v8i2.858

Abstract

Objective: To evaluate the accuracy of platelet parameters—platelet count (PC), mean platelet volume (MPV), and platelet distribution width (PDW)—as predictors of preeclampsia severity. Methods:A case-control study was conducted on 153 pregnant women with preeclampsia at Arifin Achmad Hospital from January 2019 to December 2020. Platelet indices (PC, MPV, PDW) were compared between 61 patients with severe preeclampsia and 92 with non-severe preeclampsia. ROC analysis determined predictive accuracy, with sensitivity and specificity calculated for selected cut-off values. Results:Significant differences were found in PC (p=0.000), PDW (p=0.049), and MPV (p=0.012) between groups. ROC analysis showed PDW >12.3% had a sensitivity of 60.7% and specificity of 59.8%, while MPV >10.65 fL had a sensitivity of 62.3% and specificity of 64.1%. AUC values for PDW and MPV were 0.62 and 0.66, respectively, indicating weak predictive accuracy. Conclusion:Although platelet parameters differ significantly between preeclampsia severity groups, their predictive value is limited. They should not be solely relied upon for assessing preeclampsia severity. Akurasi Parameter Trombosit (PC, MPV, dan PDW) sebagai Prediktor Tingkat Keparahan PreeklampsiaAbstrak Tujuan: Penelitian ini dilaksanakan pada pasien preeklampsia untuk mengevaluasi akurasi parameter trombosit—jumlah trombosit (PC), volume trombosit rata-rata (MPV), dan lebar distribusi trombosit (PDW)—sebagai prediktor tingkat keparahan.Metode: Studi kasus-kontrol dilakukan pada 153 ibu hamil dengan preeklamsia di Rumah Sakit Arifin Achmad dari Januari 2019 hingga Desember 2020. Indeks trombosit (PC, MPV, PDW) dibandingkan antara 61 pasien dengan preeklamsia berat dan 92 pasien dengan preeklamsia tanpa gejala berat. Analisis ROC digunakan untuk menentukan akurasi prediktif, dengan perhitungan sensitivitas dan spesifisitas berdasarkan nilai cut-off tertentu. Hasil: Terdapat perbedaan signifikan pada PC (p=0.000), PDW (p=0,049), dan MPV (p=0.012) antara kedua kelompok. Analisis ROC menunjukkan bahwa PDW >12.3% memiliki sensitivitas 60.7% dan spesifisitas 59.8%, sedangkan MPV >10.65 fL memiliki sensitivitas 62.3% dan spesifisitas 64.1%. Nilai AUC untuk PDW dan MPV adalah 0.62 dan 0.66, menunjukkan akurasi prediktif yang lemah. Kesimpulan: Meskipun parameter trombosit menunjukkan perbedaan signifikan antara kelompok preeklamsia berat dan tidak berat, nilai prediktifnya terbatas. Parameter ini tidak dapat digunakan secara mandiri untuk menilai tingkat keparahan preeklamsia. Kata kunci: Preeklamsia, Jumlah Trombosit, Lebar Distribusi Trombosit, Volume Trombosit Rata-rata, Biomarker Prediktif
Risk Factor Analysis of Overnutrition Among Elementary School Children in Pekanbaru: An Urban-rural Perspective Arfianti, Arfianti
Kesmas Vol. 13, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The prevalence of childhood overnutrition is increasing in many parts of the world including Indonesia. National basic health research (RISKESDAS) 2010 showed that over-nutrition in urban children (10.4%) was greater than in rural areas (8.1%). The purpose of this study was to analyse risk factors for overnutrition on elementary students in urban and rural areas in Pekanbaru. This was an observasional analytical study with cross-sectional design. This study involved 137 urban and 113 rural students from six elementary schools in Pekanbaru recruited by quota sampling technique. This study was conducted between April-May 2018. The results showed that the proportions of overweight and obesity in urban Pekanbaru were 16.1% and 16.8% respectively, while in rural areas were 12.4% and 9.7%, respectively. Furthermore, outdoor activity and snacking habit were significant contributors of childhood overnutrition in urban area whereas frequency of main meal was associated with childhood overnutrition in rural area. In conclusion, there was no difference in the incidence of overnutrition among elementary students in urban and rural areas in Pekanbaru. This study indicated that obesity risk factors may be different between urban and rural areas in Pekanbaru.
Co-Authors Andi Zainal Ardini, Nadiah Yamanza Ariza Julia Paulina Armi, Wahyuli Bayu Fajar Pratama Bebe Gani Dani Rosdiana Darmawi Darmawi Dedi Afandi Deyana, Frilly Dita Kartika Sari Djojosugito, Fauzia A. Elda Nazriati Erika Erika Fauzia Andrini Fifia Chandra Fifia Chandra Hamidy, Muhammad Y. Hendra Asputra Hendri, Sherin Alifia Huriatul Masdar Ibrahim, Sundus Indrati, Agnes R. Irham, Lalu M. Ismawati Ismawati Ismawati Ismawati Kesumah, Dewi Iramayana Sandra Khairunisa, Siti Q. Labecka, Magda Lazuardi Umar Maisaroh Maisaroh Muhammad Beni Septima Nababan, Heru Maranatha Nasronudin Nasronudin Nukman Moeloek Nurfi Pratiwi, Nurfi Nurul Humairah Arfiza Piki Setri Pernantah Purnomo Soeharso Rachman, Brian E. Rahmat Azhari Kemal Rahmondia N. Setiadi Ramadhani, Nur'aina ID Rangkuti, Ina F. Reni Oktora Riefni Silara Dini Rina Amtarina Rita Endriani Rudi Wisaksana S, Donel S.Pd. M Kes I Ketut Sudiana . Saputra, Nicko Pisceski Kusika Sherin Alifia Hendri Simanjuntak, Arya Marganda Siregar, Fajri M. Soraya Soraya Suri Dwi Lesmana Suyanto Suyanto TB Odih Rhomdani Wahid Tetty M Linda Tri Muhti Puja Kesuma Ulfah Ulfah Ulfah Ulfah Winarto Winarto Winarto Winarto Wirawan Adikusuma Yuhendri, Vitriyanna Mutiara Yulia Irvani Dewi Yulia Wardany Yuliati Yuliati Zarfiardy Aksa Fauzi