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All Journal HAYATI Journal of Biosciences MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management BIOTROPIA - The Southeast Asian Journal of Tropical Biology JURNAL PEMULIAAN TANAMAN HUTAN AGRIVITA, Journal of Agricultural Science Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) REINWARDTIA Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Journal of Chemistry Action Research Literate (ARL) Journal Of Sustainability Perspectives Journal of Sustainability Perspectives Global Forest Journal Policy Brief Pertanian, Kelautan, dan Biosains Tropika Reinwardtia Indonesian Journal of Forestry Research
Fifi Gus Dwiyanti
Department Of Silviculture, Faculty Of Forestry And Environment, IPB University, IPB Darmaga Campus, Dramaga, Bogor, 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Veterinary

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Microsatellite Identification Based on Genome Assembly Reveals Potentials Marker of Macassar Ebony (Diospyros celebica Bakh.) Muhammad Majiidu; Deden Derajat Matra; Fifi Gus Dwiyanti; Iskandar Z Siregar
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 14 No 2 (2024): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.14.2.399

Abstract

Macassar ebony (Diospyros celebica Bakh.) is an endemic tree species native to Sulawesi. To date, there are limited comprehensive publications regarding its genome assembly. In this study, we employed paired-end libraries of HiSeq 4000 Illumina, generating 141.2 million paired-end reads (42.4 Gigabases). Our analysis revealed the presence of 950,081 Scaffolds, with an N50 value of 6,023. Notably, BUSCO analysis identified 183 (12.7%) complete and single-copy BUSCOs (S), as well as 9 (0.6%) complete and duplicated BUSCOs (D). Furthermore, we identified 12,890 microsatellites within the Macassar Ebony genome. These microsatellites encompass 14 dinucleotide SSR motifs, 12,090 trinucleotide SSRs, 780 tetranucleotide SSR motifs, and 6 pentanucleotide SSRs. This dataset represents a valuable resource for assessing Macassar Ebony genetic makeup in its natural habitats and for subsequent analyses of the Macassar Ebony genome.
Co-Authors Adzkia, Ulfa Alfi Hudatul Karomah Alfian Helmi Amigunani, Zahra Wajdini Amperanoto, Agus Arida Susilowati Iswanto Arief Sabdo Yuwono Arrofaha, Nawwall Aulia Andi Mustika Ayyasy, Yahya Boedi Tjahjono Budi Purwanto Cut Rizlani Kholibrina Deden Derajat Matra Dien Atin Boritnaban Dodik Ridho Nurrochmat Dwi Utami , Anisa Febrita, Joana Handian Purwawangsa Henti Hendalastuti Rachmat Henti Rosdayanti, Henti Hudaya, Aang Husin Alatas IBNUL QAYIM Ida Mallia Ginting IPB, BPKB Irsyad Kamal Iskandar Z Siregar Iskandar Z. Siregar ISKANDAR ZULKARNAEN SIREGAR Khairunnisa, Zayyaan Nabiila Khoirunisa, Hana Ko Harada Ko Harada Ko Harada Koichi Kamiya Koichi Kamiya Koichi Kamiya Kustiyarini, Nur Fadila KUSUMADEWI SRI YULITA Majiidu, Muhammad Meti Ekayani Mohamad Rafi Muhammad Majiidu Muhammad Majiidu Muhammad Majiidu Muharam, Karima Fauziah Mutya Kana Purba Nyoto Santoso Putra, Heriansyah Rachmat, Henti Hendalastuti Rahadian Pratama Rahman, Mohamad Miftah Rai Sita Rakhmad Fadillah Rifnadhi, Bayu RIKA RAFFIUDIN Rina Mardiana Rukmana, Adi Sari, Windi Mayang Septaningsih, Dewi Anggraini Siska Nurfajri Slamet, Alim S Susila, Susila Ulfah Juniarti Siregar Utami, Anisa Dwi Utami, Annisa Dwi Wati, Ridha WINDRA PRIAWANDIPUTRA, WINDRA Zaenal Abidin