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All Journal HAYATI Journal of Biosciences MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management BIOTROPIA - The Southeast Asian Journal of Tropical Biology JURNAL PEMULIAAN TANAMAN HUTAN AGRIVITA, Journal of Agricultural Science Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) REINWARDTIA Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Journal of Chemistry Action Research Literate (ARL) Journal Of Sustainability Perspectives Journal of Sustainability Perspectives Global Forest Journal Policy Brief Pertanian, Kelautan, dan Biosains Tropika Reinwardtia Indonesian Journal of Forestry Research Journal of Pharmascience Biodiversitas Journal of Biological Diversity
Fifi Gus Dwiyanti
Departement Of Silviculture, Faculty Of Forestry And Environment, Institut Pertanian Bogor. Jl. Lingkar Akademik Kampus Dramaga, Babakan, Bogor 16680, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Veterinary

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Microsatellite Identification Based on Genome Assembly Reveals Potentials Marker of Macassar Ebony (Diospyros celebica Bakh.) Majiidu, Muhammad; Matra, Deden Derajat; Dwiyanti, Fifi Gus; Siregar, Iskandar Z
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 14 No 2 (2024): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.14.2.399

Abstract

Macassar ebony (Diospyros celebica Bakh.) is an endemic tree species native to Sulawesi. To date, there are limited comprehensive publications regarding its genome assembly. In this study, we employed paired-end libraries of HiSeq 4000 Illumina, generating 141.2 million paired-end reads (42.4 Gigabases). Our analysis revealed the presence of 950,081 Scaffolds, with an N50 value of 6,023. Notably, BUSCO analysis identified 183 (12.7%) complete and single-copy BUSCOs (S), as well as 9 (0.6%) complete and duplicated BUSCOs (D). Furthermore, we identified 12,890 microsatellites within the Macassar Ebony genome. These microsatellites encompass 14 dinucleotide SSR motifs, 12,090 trinucleotide SSRs, 780 tetranucleotide SSR motifs, and 6 pentanucleotide SSRs. This dataset represents a valuable resource for assessing Macassar Ebony genetic makeup in its natural habitats and for subsequent analyses of the Macassar Ebony genome.
Optimizing DNA Extraction Methods from Leaf and Wood Tissues to Support Dipterocarp Conservation and Sustainable Forest Management Arrofaha, Nawwall; Rachmat, Henti Hendalastuti; Dwiyanti, Fifi Gus; Adinugroho, Wahyu Catur; Siregar, Iskandar Zulkarnaen; Kamal, Irsyad; Syaputra, Dhika; Salamah, Andi
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 16 No 1 (2026): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.16.1.13

Abstract

Dipterocarpaceae are economically important, contributing over 85% of Indonesia's timber exports. However, this crucial resource is increasingly threatened by illegal logging, habitat destruction, and the illegal timber trade, which jeopardize dipterocarp population. Furthermore, conservation efforts utilizing genetic and forensic techniques often encounter substantial challenges due to the complexities in DNA extraction protocol. To address this, the study aimed to enhance the efficiency of DNA extraction methodologies by comparing two methods: the modified cetyltrimethylammonium bromide (CTAB) and the Genomic DNA Mini Kit (Plant) from Geneaid Biotech Ltd. The research focused on leaf and wood samples from two species, spesifically Rubroshorea leprosula (Miq.) P.S.Ashton & J.Heck and Shorea laevis Ridl. For each of these species, five leaf and five wood samples were extracted using both methods. The quality of the DNA extraction was evaluated using electrophoresis and quantified with a Qubit fluorometer. Higher DNA concentrations were obtained with the modified CTAB method compared to the GeneAid kit for both R. leprosula and S. laevis, particularly in leaf tissue. The GeneAid kit consistently exhibited low DNA yield efficiency compared to the modified CTAB method for both species. Additionally, PCR amplification of both leaf and wood samples confirmed that the extracted DNA was suitable formolecular analyses. These findings not only contributed to laboratory applications but also served as practical tools for species identification and genetic conservation for sustainable forest management and law enforcement.
Assessment of Water Quality and Pollution Load Capacity in a Taluduyunu River, Gorontalo Province: The Impact of Artisanal Gold Mining Activities Sofian Rodiana; Alinda Fitriany Malik Zain; Fifi Gus Dwiyanti
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 16 No 4 (2026): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.16.4.511

Abstract

Artisanal small-scale gold mining (ASGM), locally known as PETI (Penambangan Emas Tanpa Izin), has substantially altered the upstream Taluduyunu River in Pohuwato Regency, Gorontalo Province, a reach supplying drinking water to roughly 19,686residents through PDAM Tirta Maleo. To quantify the scale of this degradation, water quality was assessed at four stations across the disturbance gradient during the 2023 dry season, covering 24 physicochemical parameters against Class II standards (PP 22/2021). Among all parameters measured, only total suspended solids (TSS) exceeded regulatory thresholds. At SR3, the most intensively mined station, TSS reached 21,000 mg/L, 420 times the 50 mg/L Class II limit, while BOD and COD remained below detection limits at all locations. The pollution index (PI) placed SR3 in the heavily polluted category (PI = 10.01) and SR2 and SR4 in the moderately polluted range. Actual TSS loads exceeded assimilative capacity (TMDL) by approximately 100-fold: BPA = 5,237,913.6 kg/day againstBPM = 52,272 kg/day, leaving a deficit of roughly 5.2 million kg/day and requiring a 99% load reduction for compliance. Multi-stakeholder analytic hierarchy process (AHP) analysis ranked TMDL determination (weight 0.341) and river classification (0.264) as themost urgent interventions, ahead of community outreach, monitoring, and law enforcement. The combined TMDL–AHP approach developed here may transfer to other ASGM-impacted watersheds, contributing to SDG 6 and SDG 15 objectives.
Co-Authors Adinugroho, Wahyu Catur Adzkia, Ulfa Agus Hikmat AINUN FADILAH Alfi Hudatul Karomah Alfian Helmi Alim S Slamet Alinda Fitriany Malik Zain Amigunani, Zahra Wajdini Amperanoto, Agus Andi Salamah Ari Sri Windyaswari ARIDA SUSILOWATI ARIDA SUSILOWATI ARIDA SUSILOWATI Arida Susilowati Iswanto Arief Sabdo Yuwono Arrofaha, Nawwall ASEP HIDAYAT ASEP HIDAYAT Aulia Andi Mustika Ayu, Inna Puspa Ayyasy, Yahya Boedi Tjahjono Budi Purwanto Cecep Kusmana Cut Rizlani Kholibrina Deden Derajat Matra DENI ELFIATI Dien Atin Boritnaban Dodik Ridho Nurrochmat Dwi Utami , Anisa Fahrauk Faramayuda FAUZAN ALAWI Febrita, Joana Hana Khoirunisa Handian Purwawangsa HENTI HENDALASTUTI RACHMAT HENTI HENDALASTUTI RACHMAT HENTI HENDALASTUTI RACHMAT Henti Hendalastuti Rachmat Henti Rosdayanti, Henti Heriansyah Putra Heriansyah Putra Hudaya, Aang Husin Alatas IBNUL QAYIM IDA MALLIA GINTING Ida Mallia Ginting IPB, BPKB Irsyad Kamal ISKANDAR Z. SIREGAR Iskandar Z. Siregar ISKANDAR ZULKARNAEN SIREGAR Istomo . IWAN HILWAN Jeprianto Manurung KESUMA WIJAYA Khairunnisa, Zayyaan Nabiila Khoirunisa, Hana Ko Harada Ko Harada Ko Harada KOICHI KAMIYA KOICHI KAMIYA Koichi Kamiya Koichi Kamiya Koichi Kamiya Kustiyarini, Nur Fadila KUSUMADEWI SRI YULITA KUSUMADEWI SRI YULITA KUSUMADEWI SRI YULITA Laswi Irmayanti, Laswi Lina Karlinasari Majariana Krisanti Majiidu, Muhammad Maria Tri Miarsih Matra, Deden Derajat Meti Ekayani Mohamad Rafi Muhammad Majiidu Muhammad Majiidu Muharam, Karima Fauziah Mutya Kana Purba NAWWALL ARROFAHA NELLY ANNA Niken Tunjung Murti Pratiwi NUR MUFARHATUN Nyoto Santoso Putra, Heriansyah Rachmat, Henti Hendalastuti Rahadian Pratama Rahman, Mohamad Miftah Rai Sita Rakhmad Fadillah Rifnadhi, Bayu RIKA RAFFIUDIN Rina Mardiana Ritonga, Faujiah Nurhasanah Rukmana, Adi Salsa Rizqyana Billa Sari, Windi Mayang Septaningsih, Dewi Anggraini Siska Nurfajri Slamet, Alim S Sofian Rodiana SUSILA SUSILA Susila, Susila Syaputra, Dhika Triadiati Triadiati Ulfah Juniarti Siregar Utami, Anisa Dwi Utami, Annisa Dwi Wati, Ridha WINDRA PRIAWANDIPUTRA, WINDRA Yosie Syadza Kusuma Zaenal Abidin Zayyaan Nabiila Khairunnisa Zulfikar Malik