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All Journal Jurnal Biologi Tropis Jurnal Analis Kesehatan Jurnal Biomedika Jurnal Ilmiah Tatengkorang GEMASSIKA: Jurnal Pengabdian Kepada Masyarakat Jurnal Peduli Masyarakat Sciscitatio : Journal for Biological Science Jurnal Farmasi Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Jurnal Kesehatan Kusuma Husada Pharmaqueous Journal of Environment and Sustainability Education Prosiding Seminar Nasional Unimus
Yusianti Silviani
Diploma III Program In Medical Laboratory Technology, National Health Science Institute (STIKES Nasional)l
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Physics Public Health Other

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Identification of Neisseria Gonorrhoeae in Female Sexual Workers (FSW) Localized on Baai Island, Bengkulu City Lenzu, Henrek Miko; Silviani, Yusianti
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9152

Abstract

Sexually Transmitted Infections (STIs) pose a significant global health challenge, particularly among high-risk populations such as female sex workers (FSWs). Neisseria gonorrhoeae, a causative agent of gonorrhea, is a major concern due to its prevalence and transmission via sexual contact. This study aims to identify the presence of Neisseria gonorrhoeae in female sex workers (FSWs) at the Pulau Baai locality, Bengkulu City, and examine the correlation between infection rates and factors such as duration of work and frequency of client visits. A descriptive observational study with a cross-sectional design was conducted, involving 31 FSWs who participated in the study after providing informed consent. Samples were collected through vaginal swabs and examined using Gram staining to identify Neisseria gonorrhoeae. The findings revealed that 3.2% (1 out of 31) of the participants tested positive for Neisseria gonorrhoeae. Statistical analysis showed that the risk of infection increased with the duration of work, as well as the frequency of client visits. The study concludes that while the overall infection rate is low, the prevalence of Neisseria gonorrhoeae remains a concern in this high-risk group. Regular screenings and preventive measures, including consistent condom use, are recommended to reduce transmission among FSWs.
UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN PALA (Myristica fragrans Houtt) TERHADAP Staphylococcus epidermidis Wandan Wisdyafanny, Marcelina; Silviani, Yusianti
Jurnal Farmasi (Journal of Pharmacy) Vol. 12 No. 1 (2023): Jurnal Farmasi (Journal of Pharmacy), April 2023
Publisher : Sekolah Tinggi Ilmu Kesehatan Nasional & Pengurus Cabang Ikatan Apoteker Indonesia (IAI) Sukoharjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.373013/050nm603

Abstract

Acne vulgaris or acne can be caused by bacteria, one of them is Staphylococcus epidermidis which will produce lipolytic enzymes that turn sebum into a solid mass, then it will clog the ducts of the sebaceous glands. Nutmeg leaves contain saponins, triterpenoids, tannins, and flavonoids used as an antibacterial. The purpose of this study was to determine the inhibition of the ethanol extract of nutmeg (Myristica fragrans Houtt) leaves on the growth of Staphylococcus epidermidis. This study used a descriptive experimental research method to determine the antibacterial effectiveness of the ethanol extract of nutmeg (Myristica fragrans Houtt) leaves against Staphylococcus epidermidis. The data source for this study was primary data based on the results of the inhibition zone on Staphylococcus epidermidis by administering ethanol extract of nutmeg (Myristica fragrans Houtt) with concentrations of 20%, 40%, 60%, 80%, and 100%. The results showed that the ethanol extract of nutmeg leaves could be used as an antibacterial against Staphylococcus epidermidis. The average diameter of the inhibition zone of nutmeg leaf extract formed at 20% concentration was 10,45 mm, at 40% concentration was 11,4 mm, at 60% concentration was 11,9 mm, at 80% concentration was 12,35 mm, and at a concentration of 100% that is equal to 12,6 mm. The concentration of 100% was able to inhibit Staphylococcus epidermidis with the widest average diameter of the inhibition zone, which was 12,6 mm.
Difference Results Bacteria Numbers with Variations Temperature and Check Time in The Elderly Urine Suspect Urinary Tract Infections (UTI) Septiani, Risma Dyah; Silviani, Yusianti
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6723

Abstract

Delays in examination have the potential to result in differences in the results of the number of bacteria in the examined specimens plus storage of specimens at non-optimal temperatures. The difference in the number of bacteria can be known by examining the Total Plate Count (TPC) in urine. The purpose of this study was to determine the differences in the results of germ numbers in terms of variations in examination delays and urine storage temperatures of elderly suspects with urinary tract infections (UTI).  The type of research conducted was experimental analytic with the subject of 4 elderly urine in Kratonan Village, Surakarta with criteria sampling technique. Research data were obtained through questionnaires and examination of total plate numbers with mayo technique examination, where the results were multiplied by a conversion factor of 102 CFU/ml.  The results were analyzed by SPSS statistical test using Two Way ANOVA which obtained a significant value of 0.915 (p>0.05). Then continued with SPSS data analysis using Tukey Pos Hoc aims to see the real difference of each variation. There is no difference in the germ count results with temperature variations and time delays in the urine of elderly suspected urinary tract infection (UTI) patients.
Microscopic Examination of Acid-Resistant Bacils in Closed Contacts of Patients With Tuberculosis in The Working Area Of The UPTD Puskesmas Cilegon Rahayu, Ayu Puji; Silviani, Yusianti
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.6980

Abstract

Tuberculosis is a contagious disease caused by Mycobacterium tuberculosis. The transmission of pulmonary TB occurs by the emission of droplets who are positive for the disease. Attempts were undertaken to examine interactions between cadres and the community. The patient's close contacts underwent microscopic inspection utilising the Ziehl Neelsen staining procedure. Between 2023 and October, a total of 360 cases of tuberculosis will be investigated using the ZN painting method. Among the close contacts of the 72 patients, 20% (6 individuals) will be identified as having tuberculosis. This study aimed to assess the outcomes of BTA microscopic examination conducted on close household contacts within the operating region of UPTD Puskesmas Cilegon in 2024. The research method employed a descriptive observational approach with a cross-sectional design. The study was carried out from 1 February to 9 March 2024 at the UPTD Puskesmas Cilegon. The sample technique employed is purposive sampling. The study findings revealed that out of the total number of respondents, 32 individuals reported having direct contact with individuals affected by tuberculosis. Among these respondents, 13 (40.6%) were male and 19 (59.4%) were female. Out of the 32 individuals who had close contact, one respondent (3.3%) tested positive for BTA when microscopic inspection was conducted. The proximity to the afflicted individual directly correlates with the heightened likelihood of transmission. Proximity between those who are infected and their close contacts can lead to the transmission of the infection, even when sharing the same sleeping space.
Antibacterial Efectiveness Test of Basil Leaf Ethanol Extract (Ocinum sanctum L) Against Streptococcus pneumoniae ATCC 6301. Khasanah, Barhoroh Nurul; Silviani, Yusianti
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8601

Abstract

Basil leaves, which include flavonoids, alkaloids, saponins, tannins, and phenols, are one of the herbal plants that may be used as an antibiotic. We set out to see whether our hypothesis that an ethanol extract of basil leaves inhibited the development of Streptococcus pneumoniae ATCC 6301 bacteria would hold water. Methods from descriptive experimental research are employed in this work. As part of its Bacteriology Laboratory, the National College of Health Sciences carried out the investigation. Quantitative sampling is the method employed. Foliage picked fresh from a basil field in Klaten's Jogonalan neighborhood. Powdered basil leaves were isolated by maceration with 96% ethanol. A series of concentrations of 20%, 40%, 60%, 80%, and 100% DMSO were subsequently applied to the extract. A Kirby-Bauer assay for determining antimicrobial efficacy. Findings showed that an extract from basil leaves was antibacterial against the strain of Streptococcus pneumoniae ATCC 6301. On average, the inhibition zone diameter for basil leaf extract at 0%,40%,60%,80%, and 100% was 10.4 mm, 10.8 mm, 11.5 mm, 11.9 mm, and 13.0 mm, respectively. A 13.0 mm bland zone was generated by the maximum effective concentration of 100% in inhibiting Streptococcus pneumoniae ATCC 6301. No matter the dose, the antibacterial effects of basil leaf extract (Ocimum x africanum Lour) were able to suppress the growth of Streptococcus pneumoniae ATCC 6301. able to block 13.0 mm. C.
Perbedaan Interpretasi Hasil Bakteri Tahan Asam Berdasarkan Faktor yang Mempengaruhi Pemeriksaan di Puskesmas Bandar Lampung Sari, Aprilia; Silviani, Yusianti
Pharmaqueous: Jurnal Ilmiah Kefarmasian Vol. 5 No. 2 (2023): Volume 5, Nomor 2, November 2023
Publisher : Universitas Al-Irsyad Cilacap

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36760/jp.v5i2.318

Abstract

Tuberkulosis (TB) adalah penyakit menular dengan angka kematian sebesar 34 per 100.000 penduduk atau atau setara dengan 11 kematian/jam dan merupakan salah satu ancaman yang mematikan yang disebabkan oleh bakteri Mycrobacterium tuberculosis. Salah satu pengendalian TB Paru adalah mampu mendeteksi kasus TB secara dini. Saat ini pemeriksaan Mikroskopis BTA (Bakteri Tehan Asam) yang banyak digunakan masih memiliki kelemahan dalam mendeteksi yang efektif. Perkembangan teknologi telah mampu mendeteksi Tuberkulosis (TB) dengan pemeriksaan TCM (Tes Cepat Molekuler) GeneXpert® MTB/RIF. Tujuan dari penelitian ini adalah mengetahui tingkat perbedaan hasil pemeriksaan Tuberkulosis (TB) metode Mikroskopis dibandingkan dengan hasil pemeriksaan TCM (Tes Cepat Molekuler) GeneXpert® MTB/RIF berdasarkan uji deoxyribonucleic acid (DNA) untuk mendeteksi bakteri Tuberkulosis (TB). Desain penelitian yang digunakan dalam penelitian ini menggunakan analitik eksperimental dengan pendekatan cross-sectional. Sampel pada penelitian ini sebanyak 30 sampel diambil secara kuota sampling. Sampel diperiksa menggunakan metode Mikroskopis dan TCM. Dari hasil penelitian menunjukkan tidak ada perbedaan interpretasi hasil pemeriksaan Bakteri Tahan Asam metode Mikroskopis dengan TCM (Tes Cepat Molekuler) GeneXpert® MTB/RIF, ada perbedaan hasil mikroskopis berdasar Konsistensi sampel.
Potential of Kecombrang (Etlingera elatior) Flower Ethanol Extract as an Anti-Bacterial for Escherichia coli and Pseudomonas aeruginosa in Urinary Tract Infection Patients Zahra, Angelique Fadhilah; Silviani, Yusianti
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10352

Abstract

Pseudomonas aeruginosa and Escherichia coli are the two bacteria that produce one of the most prevalent urinary tract infections (UTIs). The rising resistance to antibiotics in these bacteria has led to the need for alternative treatments. Using natural materials, such as torch ginger flowers (Etlingera elatior), which are known to possess potent antibacterial chemicals, is one such option. This study aimed to determine the effectiveness of ethanol extract of torch ginger flower against Escherichia coli and Pseudomonas aeruginosa, and to compare its performance with ciprofloxacin as a standard control. The study was designed using a cross-sectional experimental method. The disk diffusion method on Mueller-Hinton Agar was used to assess the extract's antibacterial activity after it was made by macerating it with 70% ethanol. With an average inhibition zone of 7.8 mm against Escherichia coli and 8 mm against Pseudomonas aeruginosa at a 100% concentration, the findings demonstrated the antibacterial activity of Etlingera elatior's ethanol extract. These inhibition zones, however, were much smaller than those of ciprofloxacin, which had respective measurements of 10 mm and 34 mm. Significant differences between the extract concentrations were found by statistical analysis using the Kruskal-Wallis and Mann-Whitney tests (p < 0.05). In conclusion, the ethanol extract of torch ginger flower shows potential antibacterial activity against UTI-causing bacteria, although its effectiveness is not yet comparable to ciprofloxacin. Further research is needed to improve extraction methods and develop more effective formulations to enhance its antibacterial properties.
Blood mercury levels and PCR-based GSTP1 and GSTT1 gene profiles in artisanal gold miners: A cross-sectional study Wimpy, Wimpy; Silviani, Yusianti; Hidayati, Nur; Aribowo, Daffina Ramadhani Putri; Septiani, Erni Dwi; Masykuri, Mohammad
Journal of Environment and Sustainability Education Vol. 4 No. 1 (2026)
Publisher : Education and Development Research

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62672/joease.v4i1.159

Abstract

Mercury (Hg) exposure from artisanal and small-scale gold mining (ASGM) remains a major public health concern, particularly in communities where mercury amalgamation is still widely practiced. Chronic mercury exposure is known to induce oxidative stress and may interfere with detoxification pathways involving glutathione S-transferase enzymes, including GSTP1 and GSTT1. This study aimed to assess blood mercury levels and PCR-based GSTP1 and GSTT1 gene profiles among artisanal gold miners in Central Java. A cross-sectional descriptive study was conducted using whole blood samples from 10 adult males, consisting of 5 artisanal gold miners and 5 non-miner controls. Blood mercury concentrations were measured spectrophotometrically, while GSTP1 and GSTT1 gene profiles were evaluated using polymerase chain reaction (PCR). Blood mercury levels in miners ranged from 20.244 to 67.987 µg/L, exceeding the recommended reference value of <20 µg/L, whereas control subjects showed levels below the threshold. PCR analysis demonstrated expected GSTP1 and GSTT1 bands in all control samples. In contrast, miner samples showed variations in PCR band patterns, including larger than expected amplicons for GSTP1 and absence of GSTT1 amplification in two samples, which may indicate altered gene profiles associated with mercury exposure. In conclusion, artisanal gold miners exhibited elevated blood mercury levels alongside variations in GSTP1 and GSTT1 PCR profiles. Although the small sample size limits definitive genetic interpretation, these findings suggest that combined biomonitoring of mercury burden and detoxification-related gene profiles may provide useful preliminary indicators for assessing susceptibility to mercury exposure in ASGM settings.
Co-Authors Adisty, Candrika Andriyani - Puspitaningrum Andriyani Puspitaningrum Anjarani, Ajeng Viona Putri Anjaswari, Vivi Capawati Anjela Bella Dora Natalia Ardy Prian Nirwana Ardy Prian Nirwana, Ardy Prian Aribowo, Daffina Ramadhani Putri Astriana, Sevy Astuti Setiyani Bambang Hadi Sugito Binuko Amarseto Darjati Dedi Kurniawan Demes Nurmayanti Didik Wahyudi Didik Wahyudi Dwi Iswanto, Endro Endarini, Lully Hani Evi Yunita N Hadi Suryono Handayani, Susanti Herlinda Darmastuti Indah Lestari, Indah Indito, Adelio Szaky Putra Indriati, Elli Ira Puspita Sari Isfentiani, Dina Juliana Christyaningsih Kartika Pertiwi Khambali, Khambali Khasanah, Barhoroh Nurul Kiaonarni OW Kusumaningrum, Septiana Lembunai Tat Alberta, Lembunai Tat Lenzu, Henrek Miko Leonardo Bagus Utomo Maulandi, Andi Mohammad Masykuri Mulatningtyas, Zevanya Mutianingsih, Nur Mutiarawati, Diah Titik N. S. Widodo Niassinta, Julita Novita Sari, Dyah Ayu Nur Hatijah Nur Hidayati Nurhidayat Nurhidayat Octaviana Ikka Sandra Susanti Pembayun, Raflesia Rasmiati Putri Pengge, Nuning Marina Pramuditha, Zevanya Mulatningtyas PuriMahaliya, Karisma Puspitadewi, Teresia Retna Puspitasari, Afita Indah Putri, Alifa Purwahari Putu Salsabila Rida Okta Rahayu, Ayu Puji Rahayuningsih, Christ Kartika Rahmawati, Afrilia Retno Sasongkowati Sari, Aprilia Saroh, Dewi Septiani, Erni Dwi Septiani, Risma Dyah Septiyan, Erni Dwi Siagian, Hotmaida Siti Alfiah Suciati Sudarisman Sugiyarto Sugiyarto Suliati Sumasto, Hery Sungkar, Rizky Fadzillah Susanti Handayani Syafa, Alya Nur Tantias, Farmasiriana Deli Tri Harningsih, Tri Wahono Putri, Yordha Maharani Wandan Wisdyafanny, Marcelina Wimpy, W Yanti, Rahma Yulianti, Adhiesti Nur Yulianti, Widya Ratri Intan Yuniarta, Abdullah Burhan Zahra, Angelique Fadhilah Zuhria Nurullita