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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Silvikultur Tropika (JST) Microbiology Indonesia Agrovigor: Jurnal Agroekoteknologi Indonesian Journal of Agricultural Science Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Techno: Jurnal Penelitian Jurnal Agroteknos Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Kultivasi Jurnal Agrotek Tropika Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat
Supramana
Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor Jl. Kamper, Kampus IPB, Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences

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Journal : Jurnal Perlindungan Tanaman Indonesia

 1 
In Vitro Cultivation of Aphelenchoides besseyi Christie on Fungal Cultures Annisa Nur Imamah; Supramana Supramana; Tri Asmira Damayanti
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.42227

Abstract

White tip disease on rice caused by Aphelenchoides besseyi is categorized as emerging infectious disease in Indonesia because the disease incidence and its geographical distributionare increasing rapidly. Until presently, there is no information related with mass culture technique of Ap. besseyi in Indonesia. Thus, studies to obtain pure population of Ap. besseyi  are necessary to be conducted to support various research aspects in the future. This research is aimed to study Ap. besseyi pure mass culture technique by rearing the nematode on three fungal species. Three different fungal cultures on PDA medium, i.e. Alternaria padwickii, Fusarium semitectum, and Botrytis cinerea were tested as Ap. besseyi cultivation media. To obtain the optimum condition for nematode cultivation, the fungal cultures were incubated in three different temperature. The nematodes were surface sterilized with 0.1% streptomycin sulfate followed by washing with sterilized water before infesting the nematode into fungal culture. Subsequently, 25 sterilized nematodes were infested into 7 days old of fungal culture and incubated at 20ºC, 25ºC and 30 ºC. After 21 days, nematodes were harvested and counted the final population. Among fungal species tested as rearing medium, the best culture for Ap. besseyi reproduction was on Al. padwickii culture at 25 ºC with average final population up to 9,115 per petri dish and reproduction factor 364.6. At a 30ºC, Ap. besseyi was failed to develop its population in all the fungal cultures tested. It indicated that the optimum temperature for reproduction  of Ap. besseyi on those of  fungal  species was 20-25 ºC.
Morphological and Molecular Characteristics of Pratylenchus coffeae from the Origin of Robusta Coffee Plantation in Malang, East Java Aris Budiman; Supramana Supramana; Giyanto Giyanto
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.42481

Abstract

Pratylenchus coffeae is the most important plant-parasitic nematode in Robusta coffee plantations. Information regarding morphology, morphometric and molecular characters of P. coffeae has not been reported in Indonesia. This study is aimed to describe those characters of P. coffeae that attack Robusta coffee. Root samples were taken from Robusta coffee plantation in Malang, East Java. Nematode extractions was conducted using a mist chamber method. Morphology and morphometric characters were observed from the permanent nematode slides. Single nematode DNA extract was amplified at the D2D3 segment of 28S rRNA and ITS1-5.8S-ITS2 rRNA with universal primers. Amplicon was sequenced and analysed for phylogenetic tree relationships. Female morphological key character of P. coffeae observed are: lip with two annulations, four lateral lines, esophageal overlap with intestine ventrally, monodelphic, and truncated tail shape. Male spicules curved ventrally. Female morphometrics are: n=26, L = 556.4 μm, DGO = 2.4 μm, anterior gonad = 174.8 μm, a = 28.5, b = 6.1, b’ = 4.1, c = 20.1, c’ = 2.3, V = 81.7. A Male is smaller than a female with n=24, L = 505.9 μm,  a = 32.3, b = 5.5, b '= 3.9, c = 15.3, c' = 2.8 and T = 40.6. The molecular characters of P. coffeae were investigated for two isolates, namely SA1 and SA2.  Based on the D2D3 and ITS1-5.8S-ITS2 regions, isolate SA1 has similarity level of 99% and 97% to the P. coffeae  from NCBI. Similar result was shown by Isolate SA2 with similarity of 100% and 100% respectively. Phylogenetic tree analysis using Maximum Likelihood at the D2D3 segment of 28S rRNA and ITS1-5.8S-ITS2 regions showed that P. coffeae in this study was included in one clade with P. coffeae from several countries.
Phytonematodes Community and Polyphasic Character of Aphelenchoides varicaudatus on Garlic Plants in Tegal Regency, Central Java Mokhammad Danang Kusuma; Supramana Supramana; Giyanto Giyanto
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.49779

Abstract

The interception of Ditylenchus dipsaci, D. destructor, and Aphelenchoides fragariae, which are classified as quarantine pests, has been reported to occur on imported garlic bulbs used for consumption at several traditional markets in Bogor. This finding should increase awareness of the chance of garlic contamination in Indonesia’s garlic fields. This study aimed to evaluate the nematode community and determine polyphasic characters of Aphelenchoides species from garlic plantations. The study was conducted by sampling soil and plants from garlic plantations in Tuwel and Rembul Villages, Bojong District, Tegal Regency, Central Java in December 2018. Nematode extraction was done using a mist chamber for plant materials and sugar flotation – centrifugation methods for soil samples. Parameters measured included nematode species identity, absolute population and prominence indexes. Nematode identification was conducted based on their morphological characters. Further identification based on morphometric and molecular characters (polyphasic) conducted for nematodes species suspected as quarantine pests. Six generas of nematodes identified were Helicotylenchus, Aphelenchoides, Rotylenchulus, Aphelenchus, Criconemoides, and Tylenchus. Aphelenchoides sp. had the highest prominence value from plant tissue with a value of 6.32 and is categorized as a quarantine pest genus (A. fragariae) in garlic and was further identified to the species level. Further identification based on polyphasic characters showed the Aphelenchoides sp. found was A. varicaudatus.
Effect of Brassicaceae Waste Application on Soil Nematode Community Ibrahim, Ahmad Yusuf; Supramana, Supramana; Giyanto, Giyanto
Jurnal Perlindungan Tanaman Indonesia Vol 26, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.75321

Abstract

Brassicaceae are known to contain compounds that are biofumigants. This study aimed to determine the effect of Brassicaceae waste application on soil nematode community. Broccoli leaves and stems (Brassica oleracea var. italic), cabbage leaves and stems (B. oleracea var. capitate), kailan stems (B. oleracea var. alboglabra), radish leaves (Raphanus sativus), and leaves of kamanilan weed (Rorippa indica) were tested in this study. The total glucosinolate content of Brassicaceae waste was estimated using the palladium method with a modified spectrophotometer. The experiment was carried out in polybags containing 500 grams of soil-infested nematodes. Brassicaceae waste (15 grams/polybag) was chopped and mixed into the soil, watered, and polybags were tightly closed for 14 days. The experiment was arranged in a completely randomized design with nine treatments and five replications. The results of the total glucosinolate analysis showed that broccoli, radish, and kamanilan leaves fall into the high category (144.7-185.2 µmol/g); cabbage leaves, cabbage stems, and kailan stems fall into the medium category (52.0-56.0 µmol/g); and broccoli stems fall into the low category (35.4 µmol/g). There was no correlation between total glucosinolate contents and their effect on suppressing soil nematode communities at the applied effluent dose. The population of bacterivorous nematodes increased in each waste treatment, especially in kamanilan leaf treatment (R. indica) which reached 13,008 individuals. These results indicate that kamanilan weed has good potential to improve soil health. The treatment of Brassicaceae waste against soil nematode communities showed a low diversity index, an uneven evenness index, and a high dominance index.
Co-Authors . Giyanto Abdul Muin Adnan ABDUL MUNIF ABDUL MUNIF Abdul Munif Ade Indra Maulana Sembiring ALI NURMANSYAH Anindita, Devina Cinantya Ankardiansyah Pandu Pradana Annisa Nur Imamah Aprilyani Aprilyani Aris Budiman Aris Budiman Auliya Selamet Efi Toding Tondok Elmi Muliya Elvina Efendi Fitrianingrum Kurniawati, Fitrianingrum Gede Suastika GEDE SUASTIKA Gede Suastika Gede Suastika Giyanto GIYANTO GIYANTO GIYANTO GIYANTO Giyanto Giyanto Halimah Halimah Hamidi, Ilmi HARNI, RITA Heri Ahmadi Heriyanto Syafutra Hermanu Triwidodo Hishar Mirsam Ibrahim, Ahmad Yusuf IKA MUSTIKA IKA MUSTIKA Imamah, Annisa Nur Indahsari, Rizkhi Kikin H Mutaqin Lisnawita Lisnawita Lisnawita Lufthi Rusniarsyah Meity S. Sinaga MEITY S. SINAGA MEITY S. SINAGA Meity Suradji Sinaga Mohamad Rahmad Suhartanto Mokhammad Danang Kusuma Muhamad Taher Muhammad Jabal Nur MUSTIKA, IKA Pradana Pandu Ankardiansyah RAUF, AUNU RITA HARNI RITA HARNI RITA HARNI RITA HARNI Rita Harni RITA HARNI Salsabila, Unik Hanifah Samsudin . Sembiring, Ade Indra Maulana Sitanggang, Della SRI HENDRASTUTI HIDAYAT SUPRIADI SUPRIADI SUPRIADI SUPRIADI Supriadi Supriadi Supriadi Supriadi Syarifah Syarifah, Syarifah TRI ASMIRA DAMAYANTI Utami, Budi Sri Victoria Coo Lea Wawan Kurniawan Widodo Widodo Yundari, Yundari Zalzilatul Hikmia Hikmia