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Journal : Journal of Bioresources and Environmental Sciences

Cultivating Microalgae Botryococcus braunii in Tofu Whey Medium Marcelinus Christwardana; H. Hadiyanto
Journal of Bioresources and Environmental Sciences Vol 1, No 1 (2022): April 2022
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.14234

Abstract

Tofu waste water is still being a significant issue in Indonesia owing to its level of BOD (Biological Oxygen Demand) and COD (Chemical Oxygen Demand) (Chemical Oxygen Demand). However, this waste also includes significant ammonia (230 mg/L) which is needed for microalgae development. Microalgae are photosynthetic microorganism which need nitrogen supply for their development. Among others, microalgae Botryococcus braunii is the one with large oil amount within their cells (25 – 75 %). This study was meant to examine the usage of tofu whey for culture medium for algae development. The experiment was done by adjustment of whey volume (5-20 %) in fresh medium and algal biomass was continually measured. The findings revealed that Botryococcus braunii obtained optimum growth in 10 % volume with biomass generated at 2.4 g/L and 0,8716 g/L of lipid production. At this circumstance, COD might be lowered up to 83.33 %.
Palm Oil Milling Effluent (POME) Waste Processing by Using Microalgae Chlamydomonas sp. Riky Yonas; Uray Irzandi; Hantoro Satriadi; W. Widayat; Marcelinus Christwardana; H. Hadiyanto
Journal of Bioresources and Environmental Sciences Vol 1, No 2 (2022): August 2022
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.15152

Abstract

Along with the growth in oil palm output, the amount of trash produced will also increase. Every palm oil mill is responsible for the disposal of liquid waste known as palm oil mill effluent (POME). POME includes very high levels of BOD and COD, which may hinder the development of microalgae. Before POME may be utilized as a medium for the growth and development of microalgae, a detailed investigation is required to establish the pretreatment measures necessary to reduce the BOD and COD levels. The purpose of this investigation of POME waste as a substrate for the growth and development of microalgae is to examine the POME processing procedure utilizing wild microalgae. The experimental technique consisted of adding POME and microalgae to the Erlemeyer in accordance with the required proportion. Research demonstrates that POME pond IV waste may be utilized as a substrate for the development of wild microalgae to lower POME waste BOD and COD levels. The variables used were the ratio of POME to microalgae volume and the quantity of nutrients supplied. Microalgae growth at a ratio of 1:4 produced the greatest decreases in BOD and COD, namely 61.66 ppm and 173.33 ppm from 110.6 ppm and 496.67 ppm, respectively. The impact of adding nutrient C at a concentration of 120 ppm led to the greatest decrease of BOD and COD, namely 65.33 ppm and 186.67 ppm, whereas adding nutrient N at a concentration of 40 ppm led to the greatest reduction of BOD and COD, namely 55.41 ppm and 158.33 ppm.
A Mini Review on Technique of Milk Thermization Angela Nitia Nefasa; Marcelinus Christwardana; Zakaria Hussein Abdurrahman; Fatkur Rohman; Agus Afif
Journal of Bioresources and Environmental Sciences Vol 2, No 3 (2023): December 2023
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2023.19916

Abstract

Thermization is a preheating procedure in which milk is heated at low temperatures for a brief time prior to further processing. It is often used in the dairy processing industry to make a variety of products, including pasteurized milk, milk powder, and cheese. Eliminating psychrophilic bacteria, avoiding milk spoiling, and halting the changes generated by lipase and protease enzymes are the objectives of this method. Despite the fact that thermization enhances the shelf life of milk, it may also result in sensory changes and the germination of B. cereus spores. The goal of the thermization method is to prepare milk for the upcoming processing stage, and the temperature and length of the process might vary. Thermization does not affect the flavor of milk or dairy products such as yogurt, but it may enhance the quality of cheese by decreasing the occurrence of stale and rancid flavors. The process may be carried out in a plate heat exchanger or as a pre-pasteurization treatment for raw milk to protect milk quality during lengthy storage in insulated silos.
Effect of Storage on The Chemical Quality of Pasteurized Milk with Supplemented Soybean Oil and Phycocyanin Angela Nitia Nefasa; Ega Zahrotun Nisa; Marcelinus Christwardana
Journal of Bioresources and Environmental Sciences Vol 1, No 2 (2022): August 2022
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.14728

Abstract

The interaction between the addition of soybean oil and phycocyanin extract on the protein, fat, and solid non-fat content of pasteurized milk after a one-week storage period is highly important to investigate. Soybean oil is used to improve the quality of pasteurized milk, particularly its chemical quality, consisting of fat, protein, and Solid Non-Fat (SNF). Phycocyanin extract provides a source of protein, whereas soybean oil contains healthy fatty acids. Before the fresh milk is pasteurized, soybean oil and phycocyanin are added. The LTLT (Low Temperature Long Time) technique was used to pasteurize the milk at 63 °C for 30 minutes. After the pasteurization procedure was completed, the milk samples were refrigerated for one week at a temperature of 4 °C. Following the storage period, the chemical composition of pasteurized milk is examined. With the addition of soybean oil and phycocyanin extract, the protein content of milk rose, as shown by the findings. The sample T1 with a concentration of 0.45% soybean oil and 0.50% phycocyanin extract had the greatest amount of protein (3.58). The sample T2 with 0.45% soybean oil concentration and phycocyanin extract (1%) had the greatest fat content (6.4%). Adding soybean oil and phycocyanin extract enhanced the total SNF concentration. On the basis of the study conducted, it can be concluded that the addition of soybean oil and phycocyanin extract to pasteurized milk has an influence and interaction on the milk's protein, fat, and SNF content. The addition of soybean oil and phycocyanin extract to pasteurized milk held for one week may boost protein, fat, and SNF concentrations.
Evaluation Of Spirulina, Nannochloropsis, and Chlorella Micro-algae Growth in Palm Oil Mill Effluent (POME) Medium with Variation of Medium Types and Time Adding Nutrient Muhamad Zaini Mahdi; Yasinta Nikita Titisari; H. Hadiyanto; Marcelinus Christwardana
Journal of Bioresources and Environmental Sciences Vol 1, No 1 (2022): April 2022
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.14239

Abstract

POME is a kind of liquid waste produced by the crude palm oil industry. POME was not treated adequately, resulting in an issue for the environment owing to excessive levels of COD and BOD. Algae is a kind of bio-absorbent that may neutralize contaminants in liquid waste. Microalgae need carbon, nitrogen, and phosphorus-containing ingredients to flourish. These nutrients are necessary for photosynthetic carbon sources to be converted into biomass. POME includes a high concentration of C, N, and P; hence this research aims to investigate the potential of POME as a medium for the development of algae such as Spirulina, Nannochloropsis, and Chlorella. The experiment was carried out by varying the nutrients, water type, and time of nutrient feeding. Urea and sodium bicarbonate were the nutrition. Preparing the medium, culturing the microalgae, assessing biomass, counting the algae cells, and creating a calibration curve were the procedures in the experiment. The findings revealed that POME is the best medium for microalgae, that Spirulina grows better in POME than Chlorella and Nannochloropsis, and that providing nutrients every 2 days was better than introducing nutrients at the beginning and without adding nutrients.
Cultivation of Microalgae Spirulina platensis in Palm Oil Mill Effluent (POME) Media with Variations of POME Concentration and Nutrient Composition Fitria Yuli Anggita Sari; I Made Aditya Suryajaya; Marcelinus Christwardana; H. Hadiyanto
Journal of Bioresources and Environmental Sciences Vol 1, No 2 (2022): August 2022
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.15052

Abstract

Indonesia and Malaysia are recognized as the world's leading producers of palm oil. Along with the growth of the palm oil industry in Indonesia, the amount of Palm Oil Mill Effluent has increased. Palm Oil Mill Effluent (POME) is a liquid byproduct of the palm oil production process. POME has been treated using aerobic and anaerobic ponds to lower Chemical Oxygen Demand (COD) and Biochemical Oxygen Demand (BOD) levels, but it still includes nutrients like as C,N,P that are beneficial to the development of microalgae. On this study, Spirulina platensis was grown in POME medium with 20%, 40%, and 60% V. concentrations. Every two days, the nutrients Urea, NaHCO3, and TSP were administered. Seven days of aeration and 24-hour lights are used throughout the cultivation phase. The results indicate that POME with a 20 % concentration is the optimal medium for plant growth. Add 25 ppm Urea, 50 ppm TSP, and 200 ppm NaHCO3 for the optimal nutritional composition. At the same treatment on various medium, the highest growth rate of Spirulina platensis is determined to be µ = 0.128% per day, with an optical density of 0.648. Carbon reductions range from 83.03 % to 84.10 %, while Nitrogen savings range from 78 % to 79.55 % when POME is used as a growing medium. This study also shown that the C, N, and P concentrations of POME fall by 93 to 98 %, 99 to 99.5 %, and 92 to 97 %, respectively.
Optimization of light intensity and color temperature in the cultivation of Chlorella vulgaris culture using the Surface Response Method Marcelinus Christwardana; H. Hadiyanto; Wahyu Zuli Pratiwi
Journal of Bioresources and Environmental Sciences Vol 1, No 2 (2022): August 2022
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbes.2022.14410

Abstract

Microalgae have an important role as a source of biomass in producing energy. One type of microalgae that has the potential to be developed is Chlorella vulgaris. Several factors that affect the growth and biomass production of Chlorella vulgaris microalgae are color temperature and light intensity because they play an important role in the photosynthesis process. This study aims to influence the effect of light and color temperature and optimize these parameters using Response Surface Methodology (RSM). Two independent variables were varied: light intensity 200, 400, 600, 800, 1000 lux and color temperature 3000, 4000, 5000, and 6000 K. The results showed that the average value of Chlorella vulgaris growth was higher along with higher light intensity. At a color temperature of 4000 K, the highest biomass yield and the most negligible biomass production were found at 6000 K. At a color temperature of 4000 K, it is feasible to apply it as an alternative lighting source in the production of Chlorella vulgaris. The combination of light intensity and color temperature shows that the specific growth rate and doubling time have opposite trends where high values produce low values and vice versa. Growth in dark conditions, the specific growth rate was 0.0026 day-1, and the optimal light intensity at 600 lux treatment. ANOVA evaluation showed that color temperature greatly affected growth. Based on the optimization, the optimal specific growth rate of 00751day-1 with the conditions of light intensity and color temperature of 556 lux and 4152 K, respectively.  
Graphene oxide synthesis from coconut fiber powder using triple superphosphate catalyst and its potency for secondary battery electrode Christwardana, Marcelinus; Fania, Adhista; Khaerudini, Deni Shidqi
Journal of Bioresources and Environmental Sciences Vol 3, No 2 (2024): August 2024
Publisher : BIORE Scientia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61435/jbes.2024.19926

Abstract

Coconut fiber, considered an organic waste, emerges as a promising alternative carbon source for graphene oxide production—a material characterized by its conductive nature due to oxidation and the introduction of functional groups. The synthesis process involves carbonization with varied holding times (10, 20, and 30 minutes) and the utilization of Triple Superphosphate (TSP) and Ferrocene catalysts at concentrations of 10 wt.% and 20 wt.%. Subsequently, the sonication method is employed to enhance the electrical conductivity of graphene oxide post-carbonization. Notably, the electrical conductivity tests, conducted using a sourcemeter, revealed the optimum performance at 20 minutes of carbonization duration and a 20 wt.% TSP catalyst concentration, yielding an impressive electrical conductivity of 11,489.86 S/m. These findings underscore the significance of tailored parameters in optimizing graphene oxide synthesis for applications such as high-conductivity battery anodes.  
Co-Authors . Widayat A Abdullah Adrianus Kristyo Prabowo Adrianus Kristyo Prabowo, Adrianus Kristyo Agam Duma Kalista Wibowo Agnes Priska Tiarasukma Agnes Priska Tiarasukma Agus Afif Andin Vita Amalia, Andin Vita Andri Cahyo Kumoro Angela Nitia Nefasa Angela Nitia Nefasa Angela Nitia Nefasa Aniek Sri Handayani Athanasia Amanda Septevani, Athanasia Amanda Berkah Fajar Tamtomo kiono Boy Frando Sijabat Danny Soetrisnanto Deni Shidqi Khaerudini Dessy Ariyanti Dessy Ariyanti Didik Setiyo Widodo E. Enjarlis Ega Zahrotun Nisa Eudia Christina Wulandari Fadhilla, Putri Fania, Adhista Fatkur Rohman Fitra Pradhita Fitria Yuli Anggita Sari Gunawan Gunawan H Hadiyanto H. Hadiyanto H. Hadiyanto H. Hadiyanto H. Hadiyanto H. Hargono Hamid Hamid Hantoro Satriadi Haris, Amnan Hendrawan, Muhammad Andiri Heriyanti, Andhina Putri Heru Cahyana Huda, Muhammad Badrul I Made Aditya Suryajaya Ifa Miftahushudury Ismiyarto Ismitarto Ismojo, I. Joelianingsih . Linda Aliffia Yoshi, Linda Aliffia Linda Suyati Lisa Aditya Margaretha Praba Aulia Maulana, Achmad Yanuar Muhamad Zaini Mahdi Ngadiwiyana M.Si. S.Si. Nur Rokhati Ode Resa Fatlan Pina Tiani Purbowatiningrum Ria Sarjono Putra, Alam Eka Ratnawati Ratnawati Retno Ariadi Lusiana Rifa’atunnisa, Rifa’atunnisa Riky Yonas Rinaryadi, Kemilau Permata Hati Rizkan, Muhammad Rizki Nor Amelia S. Slamet Satrio Kuntolaksono Sidik Marsudi Singgih Hartanto Sri Widodo Agung Suedy Sudirman Sudirma Syachrir, Guntur Tony Suryo Utomo Tri Retnaningsih Soeprobowati Udi Harmoko Udi Harmoko Uray Irzandi Wahyu Zuli Pratiwi Widharyanti, Ika Dyah Yasinta Nikita Titisari Yuli Amalia Husnil Zakaria Hussein Abdurrahman