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Resistensi Antibiotik pada Escherichia coli yang Diisolasi dari Daging Ayam pada Pasar Tradisional di Kota Bogor (ANTIBIOTIC RESISTANCE IN ESCHERICHIA COLI ISOLATED FROM CHICKEN MEAT OF TRADITIONAL MARKETS IN THE CITY OF BOGOR) Connie Januari; Mirnawati Bachrum Sudarwanto; Trioso Purnawarman
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Antibiotic use in farm is spread widely to treat of poultry disease including therapy, supportive or preventive use and as afeed additive to improve chicken performance. The negative effects of antibiotic use can increase the level of bacterial resistance to antibiotics. This study aimed to investigate on antibiotic resistance in Escherichia coli isolated from chicken meat that were sold in Traditional Market of Bogor City. A total of 175 samples of chicken meat were taken by purposive sampling method, out of 175 found 50 positive samples of E. coli. The samples were subjected to E. coli examination and the isolated E. coli were tested for the antibiotic resistance using eight antibiotics, i.e., amoxicillin, cefotaxime, colistin, nalidixid acid, streptomycin, erythromycin, oxytetracillin, and tetracycline. The study was conducted by using the disk diffusion method on Muller-Hinton agar according to the Clinical and Laboratory Standards Institute guidelines. The study showed E. coli isolated from chicken meat were resistance towards amoxicilin (90%), colistin (94%), nalidixid acid (86%), streptomycin (98%), erythromycin (98%), oxytetracillin (84%), tetracycline (86%), and cefotaxime antibiotics (12%). The proportion of multidrugresistant was 99%. The higher of multidrug-resistant indicated the E. coli would be a threat to public and environmental health.

Sensitivitas dan Spesifisitas Nested Polymerase Chain Reaction untuk Mendeteksi DNA Coxiella burnetii (SENSITIVITY AND SPECIFICITY OF NESTED POLYMERASE CHAIN REACTION FOR DETECTION OF COXIELLA BURNETII DNA) Trioso Purnawarman; I Wayan Teguh Wibawan; Fachriyan Hasmi Pasaribu; Agus Setiyono; Muharam Saepulloh
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.261 KB)

Sensitivity and specificity of nested polymerase chain reaction (nested PCR) to detect Coxiella burnetii(C. burnetii) DNA were studied. The primer system which consists of external primers (OMP1 and OMP2)and internal primers (OMP3 and OMP4), was designed from the nucleotide sequence of the com I geneencoding for 27 kDa outer membrane protein and used to specifically amplify a 501 bp and 438 bp fragment.This nested PCR assay was 50 fold more sensitive than that of using PCR external primer only. TheNested PCR has a detection limit as low as 300 pg/?l. Specificity studies showed that nested PCR onlydetected C. burnetii DNA and did not happened Brucella abortus, Escherichia coli, Pseudomonas aeruginosaand Campylobacter Jejuni DNA. Nested PCR has high senstively and specificaly diagnostic method of C.burnetii as agent of Q fever disease.

ANALISIS PENDUKUNG KEPUTUSAN PENILAIAN PRESTASI KERJA BERDASARKAN SASARAN KERJA DAN PERILAKU KERJA TENAGA KEPENDIDIKAN Maulita Lutfiani; Mohamad Syamsul Maarif; Trioso Purnawarman
MIX: JURNAL ILMIAH MANAJEMEN Vol 9, No 1 (2019): MIX: Jurnal Ilmiah Manajemen
Publisher : Universitas Mercu Buana

This study aimed to 1) Analyze the implementation of employee performance target (SKP) on the assessment of the performance appraisal of educational personnel in the the Faculty of Veterinary Medicine, Bogor Agricultural University (FKH-IPB). 2) Analyze the application of work behavior to the assessment of the performance appraisal of educational personnel in FKH-IPB. 3) Formulate strategic recommendations to be made by the FKH-IPB to improve assessment of performance appraisal and work productivity of educational personnel. The scope of this research is the assessment of the performance appraisal of education personnel, particularly regarding the application of the SKP and work behavior element as an civil servant (ASN) performance appraisal system. This study used the Analytical Hierarchy Process (AHP) method by distributing questionnaires and interviews to 12 experts. The results showed that 1) Creativity and innovation need to be added in the assessment of SKP in FKH-IPB to increase the productivity of education staff the weight of SKP becomes 65.4%. 2) Improved assessment of work behavior is very good by conducting guidance and supervision of education personnel periodically through the commitment and discipline of education staff. 3) The most influential performance improvement strategy is through education.

Resistensi Antibiotik Terhadap Bakteri Escherichia coli yang Diisolasi dari Peternakan Ayam Pedaging di Kabupaten Subang, Jawa Barat (ANTIBIOTIC RESISTANCE TO ESCHERICHIA. COLI ISOLATED FROM BROILER FARMS IN SUBANG DISTRICT, WEST JAVA PROVINCE) Aji Barbora Niasono; Hadri Latif; Trioso Purnawarman
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Antibiotic resistance in avian bacterial pathogens is a common problem in the Indonesia poultry industry. The present study aimed to provide information on the present status of antibiotic resistance in Escherichia coli (E. coli) from a broiler farm environment. The research method uses a cross sectional study with a stratified random sampling pattern. E. coli was collected from litter using boot swab method at the broiler house farms in Subang District. E. coli isolates (n = 74) were obtained from 74 samples of boot swabs. The sample was isolated and identified morphologically and biochemically using the IMVIC test to obtain E. coli isolates. The test was followed by a sensitivity test for nine antibiotics, namely tetracycline, sulfamethoxazole, trimethoprim, ampicillin, nalidixic acid, ciprofloxacin, enrofloxacin, gentamicin and chloramphenicol by using the agar dilution method. The interpretation of the results of this sensitivity test refers to the Clinical and Laboratory Standards Institute CLSI VET01S (2015) and CLSI M100 (2018). Measurement of the level of knowledge is done using a structured questionnaire that has been tested for validity and reliability. E. coli was isolated from 74 samples of boot swab. 93.2% of E. coli samples were found to be MDR with the highest prevalence in four types of antibiotics at 40.5%. The most common resistances observed were against tetracycline 97.3%, sulfamethoxazole 87.8%, trimethoprim 74.3%, ampicillin 68.9%, nalidixic acid 64.8%, ciprofloxacin 45.9%, enrofloxacin 40.5%, gentamicin 28.4% and chloramphenicol 10.8%. The findings of this study revealed the high level of antibiotic resistance in broiler environment is worrisome and have negative implications for human and animal health.

Pengaruh Waktu Penyimpanan Ekstrak Rennet Abomasum Domba Lokal terhadap Kualitas Keju Trioso Purnawarman; Chairun Nisa; Karunia Maghfiroh
Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Vol. 2 No. 1 (2012): Jurnal Sains Terapan, Volume 2, Nomor 1, Tahun 2012
Publisher : IPB University

The study was aimed to examine the storage effect of rennet extracted from abomasum of local sheep on the texture and organoleptic qualities of cheese including color, flavor, salty taste, and bitter taste. Ten samples of crude rennet extract were divided into two groups which each five samples were stored for 2 weeks and 24 weeks respectively. The rennet extract was then used as milk clotting agent in cheese making process. Streptococcus thermophilus and Lactobacillus bulgaricus were used as starter in those processes. The fresh cheese was coated with beeswax and ripened for 20 days in refrigerator. The ripened cheese was analyzed for the quality. The texture was analyzed by using warner blatzer shear (INSTRON®) and the data were evaluated with completely randomized design (CRD). The result showed that both time storage of rennet extract were not significantly influence on cheese texture (P<0,05). The organoleptic tests of color, flavor, salty taste, and bitter taste were rated by 25 panelists and then the data were analyzed with Friedman test. The time storage of rennet extract resulted variation on organoleptic quality of cheese. Descriptive statistical analyses provided information that each panelist has varied preferences on cheese samples. It can be concluded that the storage of rennet extract had no significantly influence on the texture of cheese and provided variation on organoleptic test. Keywords: storage, rennet extract, cheese, organoleptic test

VERIFIKASI UJI CEPAT KOMERSIAL Escherichia coli PADA CONTOH UJI DAGING SAPI BEKU (Verification of Escherichia coli Commercial Rapid Test Kit on Frozen Meat) Yasmine Qurrota Ayunina; Trioso Purnawarman; Surachmi Setiyaningsih
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

This study was aimed to assess the performance and suitability of commercial rapid test compared to the conventional test through verification process. This study used frozen meat from laboratory routine samples which divided into five groups, those were: natural group, low bacteria level group, medium bacteria level group, high bacteria level group, and control group, each sample test performed 9 replicates. All samples were tested for E. coli by conventional method (SNI 2897:2008) and commercial rapid test method. E. coli test result from both methods was calculated as precision (relative standard deviation), sensitivity, specificity, false negative, false positive, and kappa. The results showed that the commercial kit test had good precision with relative standard deviations score was 0.103. The sensitivity, specificity, false negative, false positive, and kappa score were 94.44%, 100%, 5.56%, 0%, and 0.872, respectively, indicates an equal performance with conventional method. The t student test showed that commercial rapid test method and conventional method had suitability on natural group, low bacteria levels group and medium bacteria level group.

Escherichia coli CONTAMINATION AND ITS RESISTANCE TO ANTIBIOTICS IN SE’I MEAT Gabryella Fransina Amalo; Trioso Purnawarman; Herwin Pisestyani
Jurnal Kedokteran Hewan Vol 15, No 1 (2021): March
Publisher : Universitas Syiah Kuala

The purpose of this study was to obtain Escherichia coli isolates from 11 se’i meat sellers in Kupang City and to observe their resistance to13 types of antibiotics. Escherichia coli were isolated and identified based on SNI 2897: 2008. Antibiotic resistance of the Escherichia colii isolates was determined using the Kirby-Bauer method. The results showed that 13 isolates (39.39%) of Escherichia coli in se’i meat samples had a high level of resistance to erythromycin (100%), tetracycline (76.92%), and doxycycline (61.54%). Isolates demonstrated increased resistance to streptomycin (46.15%), cephalothin (38.46%), trimethoprim-sulfamethoxazole (38.46%), amoxicillin (30.77%), chloramphenicol (30.77%), and choline sulfate (30.77%). The antibiotics nalidixic acid and ciprofloxacin demonstrated low Escherichia coli resistance (7.69%). Escherichia coli are sensitive to cefotaxime and gentamicin. A total of 12 isolates (92.31%) experienced MDR. The presence of non-MDR and MDR resistant Escherichia coli in se’i meat can seriously threaten community health.

KARAKTERISTIK DAN PENGETAHUAN HIGIENE SANITASI PEDAGANG DAGING AYAM DI PASAR TRADISIONAL DI KOTA JAKARTA Hasudungan A Sidabalok; Denny Widaya Lukman; Trioso Purnawarman
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Tujuan penelitian ini mengetahui karakteristik pedagang daging ayam (umur, tingkat pendidikan, pengalaman berjualan, pelatihan, dan statuskepegawaian) di pasar tradisional di Kota Jakarta dan hubungan antara karakteristik tersebut dengan pengetahuan terkait higiene dan sanitasiketika berjualan daging ayam. Sebanyak 217 pedagang daging ayam di pasar tradisional di DKI Jakarta digunakan sebagai responden. Metodepenentuan sampel dengan cluster random sampling dan penentuan sampel pasar dengan probability proportional to size (PPS). Data dianalisissecara deskriptif dan hubungan antara peubah diuji dengan uji Gamma. Hasil yang diperoleh adalah hampir seluruh responden (n= 216; 99,5%)berumur di atas 21 tahun, sebagian besar responden (n= 105: 52,3%) lulus SMP dan SMA, dan kebanyakan responden memiliki pengalamanberjualan 3 tahun. Tingkat pengetahuan responden termasuk dalam kategori sedang sampai dengan baik (n= 209; 96,3%). Hasil analisis datamenunjukkan terdapat hubungan nyata antara pendidikan dengan pengetahuan (P0,05) dengan kekuatan korelasi sedang (r= 0,357), serta antara pengalaman dan pengetahuan (P0,05) dengan terdapat hubungan nyata antara pendidikan dengan pengetahuan (P0,05) mempunyai korelasi sedang (r= 0,357), serta antara pengalaman dan pengetahuan (P0,05) mempunyai korelasi lemah (r= 0,296).

Resistansi Penisilin terhadap Escherichia coli pada Susu Segar yang berasal dari Koperasi Ternak Sapi Cianjur Utara (KPSCU), Jawa Barat Dwida Agustina Suherman; Etih Sudarnika; Trioso Purnawarman
Jurnal Sain Veteriner Vol 41, No 2 (2023): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.83050

Resistansi antibiotik atau sering disebut sebagai pandemi senyap, merupakan satu diantara penyebab masalah kesehatan global yang harus diwaspadai. Susu segar merupakan media pembawa yang mudah terkontaminasi bakteri, salah satunya ialah bakteri E. coli. Antibiotik yang sering dipakai dalam pengobatan terhadap sapi adalah penisilin yaitu antibiotik golongan betalatam yang memiliki kemampuan membunuh bakteri dengan mencegah pembentukan protein dinding sel. Tujuan penelitian ini adalah untuk mendeteksi keberadaan dan prevalensi E. coli pada susu segar, serta menguji resistansinya terhadap antibiotik penisilin. Sebanyak 75 sampel susu kandang dipilih secara acak, berasal dari 6 kelompok ternak sapi perah dan dikumpulkan oleh masing-masing petugas lapang. Uji identifikasi keberadaan E. coli mengacu kepada Standar Nasional Indonesia (SNI) nomor 01-2897-2008 tentang metode pengujian cemaran mikroba pada susu, daging dan telur. Isolat bakteri yang teridentfikasi E. coli dilaukan pengujian resistansi terhadap antibiotik penisilin menggunakan metode Kirby-Bauer disk diffusion dengan penentuan standar berdasarkan Clinical and Laboratory Standards Institute (CLSI). Wawancara terstruktur dilakukan kepada 75 peternak yang diambil sampel susu kandang. Data yang diperoleh dianalisis secara deskriptif. Hasil identifikasi menunjukkan bahwa bakteri E. coli yang terdeteksi pada sampel susu kandang sebanyak 24/75 isolat (32%). Hasil uji resistansi menunjukkan bahwa sebanyak 23/24 isolat E. coli (96%) resistan terhadap antibiotik penisilin. Kesimpulan dari penelitian ini E. coli yang berhasil diisolasi dari susu kandang di wilayah KPSCU telah resistan terhadap antibiotik penisilin, sehingga dibutuhkan penerapan praktik higiene sanitasi, pengawasan penggunaan antibiotik, peningkatan pengetahuan peternak dan petugas kesehatan hewan terkait penggunaan antibiotik di lapangan.

VERIFIKASI UJI CEPAT KOMERSIAL Escherichia coli PADA CONTOH UJI DAGING SAPI BEKU (Verification of Escherichia coli Commercial Rapid Test Kit on Frozen Meat) Yasmine Qurrota Ayunina; Trioso Purnawarman; Surachmi Setiyaningsih
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5088

This study was aimed to assess the performance and suitability of commercial rapid test compared to the conventional test through verification process. This study used frozen meat from laboratory routine samples which divided into five groups, those were: natural group, low bacteria level group, medium bacteria level group, high bacteria level group, and control group, each sample test performed 9 replicates. All samples were tested for E. coli by conventional method (SNI 2897:2008) and commercial rapid test method. E. coli test result from both methods was calculated as precision (relative standard deviation), sensitivity, specificity, false negative, false positive, and kappa. The results showed that the commercial kit test had good precision with relative standard deviations score was 0.103. The sensitivity, specificity, false negative, false positive, and kappa score were 94.44%, 100%, 5.56%, 0%, and 0.872, respectively, indicates an equal performance with conventional method. The t student test showed that commercial rapid test method and conventional method had suitability on natural group, low bacteria levels group and medium bacteria level group.

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