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Trioso Purnawarman
Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner Fakultas Kedokteran Hewan Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Escherichia coli CONTAMINATION AND ITS RESISTANCE TO ANTIBIOTICS IN SE’I MEAT Gabryella Fransina Amalo; Trioso Purnawarman; Herwin Pisestyani
Jurnal Kedokteran Hewan Vol 15, No 1 (2021): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i1.18204

Abstract

The purpose of this study was to obtain Escherichia coli isolates from 11 se’i meat sellers in Kupang City and to observe their resistance to13 types of antibiotics. Escherichia coli were isolated and identified based on SNI 2897: 2008. Antibiotic resistance of the Escherichia colii isolates was determined using the Kirby-Bauer method. The results showed that 13 isolates (39.39%) of Escherichia coli in se’i meat samples had a high level of resistance to erythromycin (100%), tetracycline (76.92%), and doxycycline (61.54%). Isolates demonstrated increased resistance to streptomycin (46.15%), cephalothin (38.46%), trimethoprim-sulfamethoxazole (38.46%), amoxicillin (30.77%), chloramphenicol (30.77%), and choline sulfate (30.77%). The antibiotics nalidixic acid and ciprofloxacin demonstrated low Escherichia coli resistance (7.69%). Escherichia coli are sensitive to cefotaxime and gentamicin. A total of 12 isolates (92.31%) experienced MDR. The presence of non-MDR and MDR resistant Escherichia coli in se’i meat can seriously threaten community health.
KARAKTERISTIK DAN PENGETAHUAN HIGIENE SANITASI PEDAGANG DAGING AYAM DI PASAR TRADISIONAL DI KOTA JAKARTA Hasudungan A Sidabalok; Denny Widaya Lukman; Trioso Purnawarman
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2794

Abstract

Tujuan penelitian ini mengetahui karakteristik pedagang daging ayam (umur, tingkat pendidikan, pengalaman berjualan, pelatihan, dan statuskepegawaian) di pasar tradisional di Kota Jakarta dan hubungan antara karakteristik tersebut dengan pengetahuan terkait higiene dan sanitasiketika berjualan daging ayam. Sebanyak 217 pedagang daging ayam di pasar tradisional di DKI Jakarta digunakan sebagai responden. Metodepenentuan sampel dengan cluster random sampling dan penentuan sampel pasar dengan probability proportional to size (PPS). Data dianalisissecara deskriptif dan hubungan antara peubah diuji dengan uji Gamma. Hasil yang diperoleh adalah hampir seluruh responden (n= 216; 99,5%)berumur di atas 21 tahun, sebagian besar responden (n= 105: 52,3%) lulus SMP dan SMA, dan kebanyakan responden memiliki pengalamanberjualan 3 tahun. Tingkat pengetahuan responden termasuk dalam kategori sedang sampai dengan baik (n= 209; 96,3%). Hasil analisis datamenunjukkan terdapat hubungan nyata antara pendidikan dengan pengetahuan (P0,05) dengan kekuatan korelasi sedang (r= 0,357), serta antara pengalaman dan pengetahuan (P0,05) dengan terdapat hubungan nyata antara pendidikan dengan pengetahuan (P0,05) mempunyai korelasi sedang (r= 0,357), serta antara pengalaman dan pengetahuan (P0,05) mempunyai korelasi lemah (r= 0,296).
Efektivitas infusa buah jambu bol (Syzygium malaccense) sebagai antidiare pada mencit (Mus musculus) Santosa, Alfian; Purnawarman, Trioso; Mustika, Aulia Andi; Rahma, Anisa; Sutardi, Lina Noviyanti
Current Biomedicine Vol. 2 No. 1 (2024): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.1.21-28

Abstract

Background: Malay rose apple (Syzygium malaccense) fruit has been used for a long time as a traditional medicine for diarrhea by the community. Malay rose apple fruit contains active compounds such as flavonoids, tannins, and terpenoids and could be used as an antidiarrheal drug. Objective: This study aims to test the effectiveness of the infusion of Malay rose apple fruit as an antidiarrheal agent in mice (Mus musculus). Methods: This research uses the method of intestinal protection and intestinal transit. The experimental animals in this study were 25 mice, which were divided into 5 groups, consisting of negative control (1% Tween 80), positive control group (loperamide HCl), and treatment groups using infusion of Malay rose apple fruit at concentrations of 50%, 75%, and 100%. The parameters assessed in the intestinal protection method are the frequency of defecation and the consistency of the stool, while the intestinal transit method is the ratio of marker passages. Results: The results showed that the antidiarrheal effect occurred at all concentrations. The best concentration as an antidiarrhea is shown by infusion with a concentration of 50%. The 50% concentration group had the smallest average on defecation frequency, stool consistency, and marker passage ratio compared to the values of the other treatment groups and was significantly different (P<0.05) compared to the value in the negative control. Conclusion: It can be concluded that the antidiarrheal effect of the infusion of Malay rose apple fruit occurs at all concentrations, with the best effect at a concentration of 50%.
Potensi daun kumis kucing dalam meningkatkan hematopoiesis pada kondisi kerusakan ginjal Satyaningtijas, Aryani; Pamungkas, Joko; Sa’diah, Siti; Wientarsih, Ietje; Purnawarman, Trioso; Rini Madyastuti Purwono; Nisa, Khoirun; Nugroho, Radhitya Aryo; Hadiyanti, Cresensia Rara; Tarigan, Ronald
Acta VETERINARIA Indonesiana Vol. 11 No. 3 (2023): November 2023
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.11.3.189-195

Abstract

Kerusakan ginjal berdampak pada proses eritropoiesis karena ginjal merupakan organ utama penghasil eritropoietin (EPO). Tanaman kumis kucing secara turun-temurun sudah digunakan untuk pengobatan gangguan saluran kemih, hipertensi, dan diabetes mellitus. Tanaman ini diketahui mengandung berbagai zat aktif yang bersifat nefroprotektif, seperti flavonoid, tannin, saponin, phenol, serta terpenoid. Penelitian ini bertujuan untuk melihat potensi tanaman kumis kucing untuk memperbaiki profil eritosit pada kondisi kerusakan ginjal yang diinduksi oleh etilen glikol. Sebanyak 35 ekor tikus galur Sprague Dawley jantan berumur 7 bulan dibagi ke dalam 7 kelompok perlakuan dengan pemberian etilen glikol dan atau ekstrak kumis kucing dengan lama permberian yang berbeda. Pemberian ekstrak daun kumis kucing mampu meningkatkan jumlah eritrosit pada tikus serta menekan penurunan jumlah eritrosit saat dan setelah pemberian etilen glikol. Selain itu, pemberian ekstrak kumis kucing juga mampu menurunkan jumlah leukosit serta rasio neutrofil: limfosit yang meningkat akibat induksi etilen glikol. Ekstrak kumis kucing memiliki potensi untuk memperbaiki proses eritropoiesis dan menekan peradangan pada kasus kerusakan ginjal.
Quantification and identification of bacterial presence in salted eggs Vindriati, Zukhrufa Vista; Afiff, Usamah; Purnawarman, Trioso
Current Biomedicine Vol. 3 No. 1 (2025): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.1.43

Abstract

Objective This study aimed to quantify the total bacterial count in salted eggs and identify bacterial species that may affect their quality. Methods Fifty samples consisted of 10 fresh salted eggs that passed the candling test, 10 portions of pasta dough at 0, 3, and 6 hours, and 10 fresh salted eggs that had failed the candling test (black egg yolk). Each sample was tested in triplicate. The total bacterial count was determined using the plate count agar method, and bacterial identification was based on phenotypic analysis, which included Gram staining and biochemical tests. Results The total bacterial load in fresh salted eggs was below the maximum limit set by SNI 7388:2009 (1×105 CFU/g), whereas the pasta dough and black egg yolk exceeded this limit. The identified bacteria included Escherichia spp., Enterobacter spp., Proteus spp., Staphylococcus spp., Pseudomonas spp., and Bacillus spp. Conclusion Although the bacterial count in salted eggs meets SNI standards, the presence of potentially harmful bacteria highlights the need for enhanced hygiene and sanitation measures to be implemented during the production of salt eggs.
Safety Risk Factors of Duck Meat Sold in Bogor City annisa, rizka fajri; Purnawarman, Trioso; Pisestyani, Herwin
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.101423

Abstract

With the increasing demand for duck meat in Bogor City, this poses a risk of the presence of pathogenic bacteria throughout the food supply chain. Traditional markets are one of the places that have a high risk of contamination with pathogenic bacteria that can endanger public health. This study aims to determine the presence of Salmonella and E. coli in duck meat sold in traditional markets in Bogor City and the risk factors that influence the presence of these pathogenic bacteria. This study was a cross sectional study with a total of 45 duck meat samples obtained from 3 traditional markets in Bogor City. Samples were taken to the SKHB IPB Veterinary Public Health Laboratory for microbiological testing. Risk factors that may affect the safety of duck meat were assessed using a questionnaire. The results showed that 13 samples (29%) were positive for Salmonella and 27 samples (60%) were positive for E. coli. The presence of Salmonella and E. coli in duck meat indicates cross-contamination during slaughter, evisceration and poor sanitation and hygiene practices. Continuous monitoring and reporting of the presence of Salmonella and E. coli is needed so that duck meat consumed by the public is safe for consumption.
Co-Authors A.S. Satyaningtijas Afiff , Usamah Agus Setiyono Agustin Indrawati Aji Barbora Niasono Anisa Rahma, Anisa annisa, rizka fajri Aulia Andi Mustika Chairun Nisa Connie Januari Debby Fadhilah Pazra Denny Widaya Lukman Dwi Desmiyeni Putri Dwida Agustina Suherman Engki Zelpina Etih Sudarnika Fachriyan Hasmi Pasaribu Gabryella Fransina Amalo Hadiyanti, Cresensia Rara Hadri Latif Hasudungan A Sidabalok Herwin Pisestyani I wayan Teguh Wibawan IETJE WIENTARSIH Joko Pamungkas Karunia Maghfiroh Khoirun Nisa Kumala Andri Asari Lina Noviyanti Sutardi Madyastuti, Rini Maulita Lutfiani Melani Wahyu Adiningsih Mohamad Syamsul Ma’arif Monika Danaparamitha Andriani Muharam Saepulloh Nugroho, Radhitya Aryo Okti Nadia Poetri Rahmat Setya Adji Retno Damayanti Soejoedono Rusman Efendi Santosa, Alfian Sa’diah, Siti Septa Walyani Sudarwanto, Mirnawati Bachrum Surachmi Setiyaningsih Susanto Nugroho Syafril Daulay Syahdu Pramono Tarigan, Ronald Vindriati, Zukhrufa Vista Yasmine Qurrota Ayunina