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All Journal HAYATI Journal of Biosciences JURNAL KIMIA SAINS DAN APLIKASI Jurnal Penelitian Sains (JPS) Matematika dan Sains Medical Journal of Indonesia Jurnal Penelitian Sains (JPS) IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) BERITA BIOLOGI JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA JPSriwijaya Science and Technology Indonesia Jurnal Penelitian Sains Indonesian Journal of Chemistry Indonesian Journal of Pure and Applied Chemistry IJEMS (Indonesian Journal of Environmental Management and Sustainability) Martabe : Jurnal Pengabdian Kepada Masyarakat Seminar Nasional Lahan Suboptimal Molekul: Jurnal Ilmiah Kimia BIOVALENTIA: Biological Research Journal Jurnal Ilmiah Bakti Farmasi SWARNA Jurnal Riset Rumpun Ilmu Kesehatan (JURRIKES) Karunia: Jurnal Hasil Pengabdian Masyarakat Indonesia Journal of World Future Medicine, Health and Nursing SWARNA: Jurnal Pengabdian Kepada Masyarakat Bumi: Jurnal Hasil Kegiatan Sosialisasi Pengabdian Kepada Masyarakat
Miksusanti Miksusanti
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Sriwijaya University, Palembang, Indonesia
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Journal : Indonesian Journal of Pure and Applied Chemistry

 1 
AMPLIFIKASI PCR DOMAIN D1/D2 28S rDNA MENGGUNAKAN PRIMER ITS1 DAN ITS4 SAMPEL DNA DARI Candida tropicalis YANG DIISOLASI DENGAN METODE PENDINGINAN Hermansyah Hermansyah; Novian Sutami; Miksusanti Miksusanti
Indonesian Journal of Pure and Applied Chemistry Vol 1, No 1 (2018)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.548 KB) | DOI: 10.26418/indonesian.v1i1.26037

Abstract

The purpose of this research was to isolated DNA from the yeast C. tropicalis with freeze thawing method -200 C conducted on 3 colonies of C. tropicalis.  Each colony   threated variations of cooling, 3x15 minutes, 3x25 minutes and 3x35 minutes, to break the cell walls.  Subsequently all the samples amplified with 3 variations of PCR cycles, 15 cycles, 25 cycles and 35 cycles, after all of the samples isolated by freeze thawing method -200 C. Its was known that sample A15 has the smallest concentration of DNA yeast C. tropicalis, ie 50 µg/mL, while sample C35 had the largest concentration of DNA yeast C. tropicalis, ie 225 µg/mL. The result of the research indicated that the best condition can be reached in 3x35 minutes. On 35th cycle has clearer C. tropicalis DNA bands than the 25th and 15th PCR cycle. C. tropicalis DNA bands at 35th cycles there were 7 DNA bands were detected and bright bands on a long 35 minutes cooling. In the 25th and the 15th cycle, there was no DNA bands were detected in all samples. Based on the results obtained, the amplification process must be carried out at least 35 times cycles so that the C. tropicalis DNA bands can be detected.
Co-Authors Adi Supriyadi Adik Ahmadi Agus Syafriansyah Al Ainna Aldes Lesbani Almunadi T Panagan Almunady T. Panagan Andarmoyo, Sulistyo Annisa Amelia Asmak Asmak Azhar Kholiq Azhar Kholiq Bambang Ponco Bambang Ponco Bambang Ponco, Bambang Bambang Pontjo Priosoeryanto Betty S.L. J Betty Sri Laksmi J Betty Sri Laksmi Jenie Betty Sri Laksmi Jennie Budi Untari Budi Untari Darsil Basir Dasril Basir Dasril Basir Dasril Basir Dedi Rohendi Desnelli Desnelli Desnelli Desnelli Dian Dian Dian Dwita Maizur Dimas Nur Hidayat Elfita Eli Sahara Eli Sahara Elsa Fitria Apriani Endang Setiyowati Endang Setiyowati Endri Junaidi Eviyanti Eviyanti Fachrijal Fachrijal Fanani, Muhammad Zainal Fatma Fitra Yosi Fitrya . Fitrya Fitrya Gatot T. Mulyadi Gatot Tri Mulyadi Gatot Trimulyadi Gatot Trimulyadi Rekso Getari Kasmiarti Harian Saputra Harmida Harmida Heni Yohandini Heni Yohandini K Herlina Herlina Hermansyah Hermansyah Hermansyah Hermansyah Hermansyah Hotdelina S Hotdelina S Ina Suci Pratiwi Indah Solihah Indah Solihah Ismed Ismed Jefri Liasta Jennie, Betty Sri Laksmi Juswardi Juswardi Maidelwita, Yani Meisji Liana Sari Muharni Muharni Muntasir, Muntasir Nasir Rofiq Neza Rahayu Nike Marfinda Nirwan Syarif Nita Aminasih Nova Yuliasari Novian Sutami Novrikasari Novrikasari, Novrikasari NUNI GOFAR Rennie Puspa Novita Risfidian Mohadi Rizal Syarief Rizal Syarief Rizal Syarief Setiawan Yusuf Setiawan Yusuf Setiawaty Yusuf Sofia Sandi Sukmawati Sukmawati Susilawati Susilawati Syaflina Lamin Syafrina Lamin, Syafrina Syndi N.K Tridayona Tridayona Trimulyadi, Gatot Wiwin Wiwin Yusup Nur Khakim, Mokhamad