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All Journal HAYATI Journal of Biosciences Jurnal Pendidikan dan Pembelajaran Khatulistiwa (JPPK) BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Biotechnology Jurnal Teknologi Laboratorium BIOEDUSCIENCE Biosaintifika: Journal of Biology & Biology Education Jurnal Labora Medika Gorontalo Journal of Public Health Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Borneo Journal of Medical Laboratory Technology Narra J Quality : Jurnal Kesehatan Sureq: Jurnal Pengabdian Masyarakat Berbasis Seni dan Desain Prosiding University Research Colloquium Jurnal Kefarmasian Indonesia
Sri Darmawati
Muhammadiyah Semarang University
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Examining the prevalence and antimicrobial resistance profiles of multidrug-resistant bacterial isolates in wound infections from Indonesian patients Prastiyanto, Muhammad E.; Darmawati, Sri; Daryono, Budi S.; Retnaningrum, Endah
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.980

Abstract

The emergence of multidrug-resistant (MDR) infections in wounds is a significant public health issue. The aim of this study was to investigate the prevalence and antimicrobial resistance profiles of MDR bacterial isolates in wound infections. Through a cross-sectional study, 1035 bacterial isolates were collected from wound infection patients at Tugurejo Hospital in Semarang, Indonesia, over a three-year period (January 2020 to December 2022). Initial identification involved Gram staining and colony morphology assessment, followed by biochemical assays and antimicrobial susceptibility testing using the VITEK®2 Compact system. Gram-negative bacteria constituted the majority of isolates (60.77%, n=629). The predominant strains included were Staphylococcus spp. (30.92%, n=320), Escherichia coli (18.45%, n=191), and Klebsiella pneumoniae (13.04%, n=135). Notably, Gram-negative bacteria exhibited a significantly higher likelihood of MDR development compared to their Gram-positive counterparts (p<0.001), with Gram-negative bacteria having a 2.05 times higher probability of acquiring MDR. These findings underscore the urgent need for comprehensive surveillance of antimicrobial resistance patterns and the implementation of tailored antimicrobial stewardship programs to address the pressing public health challenge of MDR wound infections. Further research is warranted to elucidate the complex interplay of factors contributing to MDR development in wound infections, thereby informing targeted intervention strategies and improving patient outcomes.
Black-Pigmented Marine Pseudomonas aeruginosa Exhibiting Anti-Bacterial Activity against Multidrug-Resistant (MDR) Wound Infection Bacteria Prastiyanto, Muhammad Evy; Darmawati, Sri; Daryono, Budi Setiadi; Retnaningrum, Endah
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.880-890

Abstract

The urgency of multidrug-resistant (MDR) bacterial infections in wounds is a significant concern due to the high prevalence of MDR in healthcare settings. Black pigmented marine bacteria, strain PS1C, were isolated from marine sediment on Awur Beach Jepara, Central Java, Indonesia, and cultured in the laboratory. The aim of this research includes molecular identification of strain PS1C, extracting black pigment from strain PS1C, isolation of MDR bacteria from wounds, and evaluating the antibacterial activity of black pigment from strain PS1C against MDR bacteria isolates of wound infections. We examined the 16S rRNA gene sequences strain PS1C to identify the species. Then, the black pigment from PS1C was extracted using methanol: acetone (7:3) solvent. Antibacterial activity was evaluated against MDR bacteria from wounds with the microdilution method. A black-pigmented bacterium was isolated and identified as Pseudomonas aeruginosa PS1C. We found that the black pigment from P. aeruginosa PS1C can be developed as an antibacterial agent against the MDR bacterial isolate of wounds with MIC and MBC values of 6.25-12.5 mg/ml and 6.25-25 mg/ml, respectively. In conclusion, the study's findings highlight the potential of the extract of black pigment from P. aeruginosa PS1C as an antibacterial agent against wound-causing MDR bacteria and reinforce previous research into P. aeruginosa can be isolated from marine sources. Additional in vivo investigations and the identification of the antibacterial activity's mode of action are required.
BIOPROSPECT OF BACTERIAL FIBRINOLYTIC PROTEASE FROM BEKASAM OF LONGTAIL TUNA AS ANTITHROMBOTIC AGENT: LITERATURE REVIEW AND BIBLIOGRAPHY STUDY Trianes, Juwy; Ethica, Stalis N.; Afriansyah, M. Ardi; Darmawati, Sri; Rahmani, Nanik; Zilda, Dewi S.
Quality : Jurnal Kesehatan Vol. 18 No. 2 (2024): Quality : Jurnal Kesehatan
Publisher : Politeknik Kesehatan Kemenkes RI Jakarta I

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36082/qjk.v18i2.2016

Abstract

Introduction:Cardiovascular disease (CVD) is the leading cause of death worldwide, with thrombosis being a significant risk factor. Fibrinolytic proteases have potential as antithrombotic agents and could be developed into CVD drugs. Indonesia's traditional fermented products, such as fish Bekasam, are rich in microorganisms, including fibrinolytic protease-producing bacteria, yet their potential for CVD treatment is underexplored. Fish paste in Indonesia serves as a protein source and a reservoir of bacterial diversity, which could aid in discovering new antithrombotic agents. Objective: This literature review examines research trends over the past decade on fibrinolytic protease-producing bacteria in traditional fermented products, with a focus on Bekasam made from Longtail Tuna. This fermented food is an alternative source for obtaining bacteria with antithrombotic properties. Methods: Data for this review were sourced from Google Scholar, PubMed, and the dimension.ai database, using the keyword "bacterial fibrinolytic protease" from 2015 to 2024. Visualization of global research trends was performed using VOS viewer software. Results: The review found a scarcity of studies on fibrinolytic proteases from Bekasam bacteria. Lactic acid bacteria involved in Bekasam fermentation possess proteolytic enzymes that degrade fish protein into peptides and amino acids, potentially offering antithrombotic properties. This suggests natural protease sources from traditional fermented foods have significant biomedical potential. Research on fibrinolytic protease-producing bacteria from Bekasam in Indonesia is limited and requires further development. Exploring these local fermented products could yield innovative sources for thrombosis treatment
Diagnosing Urinary Tract Infection: Bacterial Count and Gram Identification Using Flow Cytometry Hayati, Mardiyah; Darmawati, Sri; Santosa, Budi; Lestari, Anak Agung Wiradewi; Limijadi, Edward Kurnia Setiawan; Felim, Jethro
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 31 No. 2 (2025)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v31i2.2309

Abstract

Urine culture is still the gold standard for Urinary Tract Infection (UTI) diagnostics, but it takes a long time and expensive. The flow cytometry is a more practical, fast, and reliable test for diagnosing UTI. The study was conducted to determine the validity of the bacterial count with UF 5000 flow cytometry compared to urine culture and to evaluate the coherence between Gram identification (BACT-INFO) results and the manual Gram stain.  This was a cross-sectional study aimed to determine the sensitivity, specificity, Positive Predictive Value (PPV), and Negative Predictive Value (NPV) of bacterial count compared to urine culture and to evaluate the coherence between BACT-INFO results with manual Gram stain. Urine from 60 patients diagnosed with suspected UTI was assessed for the ability to diagnose UTI using bacterial count from UF 5000 flow cytometry compared to urine culture and to determine the correlation between BACT-INFO with manual Gram staining results. This study was conducted from January to April 2022. Statistical analysis using the ROC curve showed that the Area Under the Curve (AUC) of the urine bacterial count was 0.856 and by setting a cut-off of 961.8/µL, the sensitivity was 84.0%, specificity 88.9%, PPV 97.7%, NPV 50.0%. The concordance of BACT-INFO compared to manual Gram staining is moderate (obtained from Cohen's Kappa coefficient value 0.3269) with a p-value of 0.0000. The most common bacterial species include Escherichia coli (40.67%), Klebsiella pneumoniae ss pneumoniae (20.33%), Proteus mirabilis (10.17%), Pseudomonas aeruginosa (8.47%), and Acinetobacter baumannii (6.78%). Urine bacterial count based on the flow cytometry method with a cut-off of 961.8/µL has sensitivity and specificity > 80% in diagnosing UTI. There is moderate concordance between BACT-INFO and manual Gram staining. The most common bacteria as the cause of UTI include Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, and Acinetobacter baumannii.
Suspect TB di Sekitar Tempat Tinggal Penderita TB Paru di Sambiroto Semarang Darmawati, Sri; Prastiyanto, Muhammad Evy; Listiyanti, Apriliya
Prosiding University Research Colloquium Proceeding of The 7th University Research Colloquium 2018: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

Tuberkulosis (TB) paru adalah penyakit infeksi yang disebabkan olehbakteri Mycobacterium tuberculosis, dan suspect TB adalah orangyang dicurigai menderita TB. Orang yang tinggal di sekitarpenderita TB berpotensi menjadi suspect (tersangka) TB. Tujuanpenelitian untuk mengetahui gambaran suspect TB di lingkungansekitar tempat tinggal 3 penderita TB Paru di wilayah Sambirotokota Semarang. Jenis penelitian adalah penelitian deskriptif, sampelsebanyak 30 suspect dari 3 titik tempat tinggal penderita TB ParuPositif, dari setiap titik tempat tinggal penderita diambil 10 orangsuspect yang masuk dalam screening penjaringan dengan kriteriaresponden adalah anggota keluarga yang tinggal serumah, tetanggadekat dan sering berkomunikasi secara langsung, kemudiandilakukan pemeriksaan mikroskopis dari dahaknya yaitu dahaksewaktu, pagi, sewaktu dengan pewarnaan metode Ziehl Nellson.Hasil pemeriksaan menunjukkan jumlah suspect Basil Tahan Asamdengan hasil positif sejumlah 4 sampel (13%) dari total 30 sampel.
Penelitian Pendahuluan: Aktivitas Antibakteri Cold Argon Plasma Jet terhadap Multi Drug Resistance (MDR) Bacteria Prastiyanto, Muhammad Evy; Maulida, Gita Rossa; Salsabila, Nia; Darmawati, Sri; Nasruddin, N
Prosiding University Research Colloquium Proceeding of The 10th University Research Colloquium 2019: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

Multi drug resistance (MDR) bacteria telah menjadi masalah kesehatan global diseluruh dunia dikarenakan penggunaan antibiotik yang tidak rasional. Tujuan dari penelitian ini untuk mengevaluasi aktivitas antibakteri cold argon plasma jet terhadap Methichillin Resistant Staphylococcus aureus (MRSA), Carbapenem Resistant Pseudomonas aeruginosa (CRPA), dan Extended Spectrum Betalactamase (ESBL) Escherichia coli. Proses uji antibakteri dilakukan dengan alat cold argon plasma jet pada jarak 1 cm dengan variasi waktu 1, 2, 3, 4, dan 5 menit. Aktivitas antibakteri cold argon plasma jet menunjukkan zona hambat tertinggi pada menit ke 5 CRPA 6,5 mm, ESBL E.coli 6,5 mm, MRSA 4 mm. Kesimpulan: cold argon plasma jet memiliki potensial sebagai agen antibakteri.
The Effect of Different Solvents on the Content of Black Cumin Seed Extract (Nigella sativa) Hariutami, Desi; Darmawati, Sri; Permana, Atna; Zuraida, Zuraida
Jurnal Kefarmasian Indonesia VOLUME 14, NUMBER 1, FEBRUARY 2024
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

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Abstract

Seeds of black cumin (Nigella sativa L.), also called "black cumin," are an herbal plant from the Ranunculaceae family. The objective of this study was to determine the yield of black cumin seed extract using 96% ethanol, N-hexane, and ethyl acetate as solvents, as well as to determine the results of phytochemical screening tests and the active ingredient content using GCMS. The stages of the research included the extraction process, phytochemical screening test, and GCMS. The study's findings on the yield of black cumin seed extract using the three solvents showed that the solvent N-Hexane produced the highest yield, at 28.09 percent. Based on phytochemical screening tests, black cumin seeds were positive for containing secondary metabolites such as alkaloids, saponins, phenolic flavonoids, triterpenoids, steroids, and glycoxides. Other potential compounds vizhexadecenoic acid, ethyl ester, Methyl (8e,11e)-8,11- octadecadienoate, 12-octadecadienoic acid (z,z)-, Grape seed oil.
PKM PELATIHAN TARI PADUPPA PADA ANGGOTA KARANG TARUNA KECAMATAN MALLAWA UNTUK MENINGKATKAN KREATIVITAS DI BIDANG SENI TARI Linda, Johar; Al Ansyari, Mas’ud Uqbah; Setianingsih, Erna; Faisal, Faisal; Darmawati, Sri
Sureq: Jurnal Pengabdian Masyarakat Berbasis Seni dan Desain Vol 4, No 2 (2025): Juli-Desember
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26858/srq.v4i2.78311

Abstract

Kegiatan Pengabdian Kepada Masyarakat (PKM) ini bertujuan untuk meningkatkan kreativitas anggota Karang Taruna Kecamatan Mallawa dalam bidang seni tari melalui pelatihan Tari Paduppa. Tari Paduppa merupakan salah satu tarian tradisional Sulawesi Selatan yang memiliki nilai-nilai filosofis dan sosial budaya tinggi, khususnya sebagai bentuk penghormatan kepada tamu kehormatan. Melalui pelatihan ini, para peserta dibekali dengan keterampilan dasar gerak tari, pemahaman makna simbolik gerakan, serta pengenalan terhadap musik pengiring tradisional. Metode kegiatan meliputi tahap sosialisasi, pelatihan teknik tari dan musik, serta evaluasi melalui penampilan bersama. Hasil kegiatan menunjukkan adanya peningkatan kemampuan peserta dalam menarikan Tari Paduppa, meningkatnya antusiasme terhadap seni tradisional, dan tumbuhnya kesadaran pentingnya pelestarian budaya lokal. Kegiatan ini diharapkan menjadi awal terbentuknya komunitas seni muda di lingkungan Karang Taruna yang berperan aktif dalam menjaga dan mengembangkan seni tari daerah.
PRODUCTION OPTIMIZATION, PARTIAL PURIFICATION, AND THROMBOLYTIC ACTIVITY EVALUATION OF PROTEASE OF Bacillus cereus HSFI-10 Ainutajriani, Ainutajriani; Darmawati, Sri; Zilda, Dewi Seswita; Afriansyah, Muhammad Ardi; Saptaningtyas, Ragil; Ethica, Stalis Norma
BIOTROPIA Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1765

Abstract

Cardiovascular disease is the primary cause of mortality in the world due to the formation of blood clots or thrombi in blood vessels. Bacterial proteases commonly function as thrombus dissolver agents in the pharmaceutical industry. Bacterial isolate HSFI-10 (Holothuria scabra Fermented Intestine-10) previously isolated from Rusip fermented sea cucumber had demonstrated thrombolytic activity. This study aimed to produce crude protease of HSFI-10 strain at an optimized incubation time and determine the thrombolytic activity of crude and dialysate proteases on A, B, AB, and O blood types. Isolate HSFI-10 was first molecularly identified and found to be Bacillus cereus with a homology level of 99.80% with Bacillus cereus strain ST06. The optimum crude enzyme was obtained after 48-h incubation with an activity of 222.52 U/mL, which increased to 438.84 U/mL after ammonium sulfate precipitation and dialysis. Clot lysis activity of crude enzymes was measured based on the gravimetry method on blood in the ABO system, showing results that ranged from 68.99% to 69.76%, while the dialysate ranged from 81.16% to 82.52%. In conclusion, partial purification of bacterial protease could increase both its specific and thrombolytic activities on human blood in the ABO system, with only 1% activity variability between A, B, AB, and O blood types.
Karakterisasi Imunogenesitas Protein Pilli Salmonella Typhi: Analisis Absorban Antibodi dan Spesifisitas dengan Western Blot pada Mencit Balb/C Hidayani, Al; Darmawati, Sri; Ramdhani , Fitria Hariati; Rahmah, Windya Nazmatur
Borneo Journal of Medical Laboratory Technology Vol. 8 No. 2 (2026): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v8i2.12477

Abstract

Protein pili berperan dalam adhesi dan bersifat imunogenik, berpotensi sebagai kandidat vaksin subunit. Penelitian ini menganalisis imunogenesitas protein pili S. typhi isolat BA07.4 melalui pengukuran absorbansi antibodi dan karakterisasi spesifisitasnya. Protein pili diisolasi dengan metode biphasic, dimurnikan, dan digunakan untuk imunisasi 30 ekor mencit Balb/C (6 kelompok: kontrol dan dosis 2, 3, 4, 5, 6 µg) secara intraperitoneal tiga kali. Kadar antibodi diukur dengan inhouse ELISA (λ 405 nm) dan spesifisitasnya dianalisis dengan Western blot. Rerata absorbansi tertinggi pada dosis 5 µg (2,827), diikuti dosis 6 µg (2,678), 4 µg (2,456), 3 µg (2,134), 2 µg (1,856), dan kontrol (0,423). Analisis One Way ANOVA menunjukkan perbedaan signifikan antar kelompok (p=0,000) dengan semua kelompok perlakuan berbeda signifikan terhadap kontrol (p<0,05). Western blot menunjukkan pita spesifik pada berat molekul 87, 58, dan 42 kDa pada kelompok dosis 5 µg, sementara kontrol tidak menunjukkan pita. Protein pili S. typhi bersifat imunogenik dengan dosis optimum 5 µg, menghasilkan antibodi yang secara spesifik mengenali sub unit protein pili 87, 58, dan 42 kDa, mendukung potensinya sebagai kandidat vaksin subunit.
Co-Authors A.A. Ketut Agung Cahyawan W Afriansyah, M. Ardi Afriansyah, Muhammad Ardi Ainutajriani, Ainutajriani Al Ansyari, Mas’ud Uqbah Al Hidayani Ana Hidayati Mukaromah Anak Agung Wiradewi Lestari Aprilia Indra Kartika, Aprilia Indra Bachti Alisjahbana Batara, Murni Budi Santosa Budi Santosa BUDI SETIADI DARYONO Cahyaningrum, Dea Galuh Daryono, Budi S. Dewi Seswita Zilda Edward Kurnia Setiawan Limijadi, Edward Kurnia Setiawan Endang Tri Wahyuni Maharani Ernanto, Aditya Rahman Ethica, Stalis N. Faisal Faisal Fandhi Adi Wardoyo Felim, Jethro Fitria Hariati Ramdhani Fuad, Hayatun Hariutami, Desi Hikmah, Annisa Nurul Jannah, Erni M Joharlinda, Joharlinda La’lang, Marselaonety LANGKAH SEMBIRING Listiyanti, Apriliya Manufandu, Anjar Richardo Mardiyah Hayati Masashi Kawaichi, Masashi Maulida, Gita Rossa NANIK RAHMANI Nasruddin, N Nur Hidayah Palimbongan, Junita Rensa Permana, Atna Prastiyanto, Muhammad E. Prastiyanto, Muhammad Evy Prastiyanto, Muhammad Evy Putri, Desty Ratna Ragil Saptaningtyas, Ragil Retnaningrum, Endah Rinaldi, Muhamad Rizqi Rosnita . Rukmi, Ai Rustiyarso . Sabar Pambudi Salsabila, Nia Setianingsih, Erna Silvestre, Richard David Sri Sinto Dewi Stalis Norma Ethica Sulaiman Ngongu Depamede Sulistyaningtyas, Ayu Rahmawati Susanti, A.Meryam Syaiful Anwar Trianes, Juwy Tulus Ariyadi Wayan T. Artama Wayan T. Artama Widya Asmara Wilson, Wildiani Windya Nazmatur Rahmah Yuwita, Anita Zilda, Dewi S. Zuraida Zuraida