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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Medika Tadulako Medical Laboratory Technology Journal Indonesian Food Science and TechnologyJournal JURNAL ANALIS LABORATORIUM MEDIK Tropical Health and Medical Research Journal Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Karya Generasi Sehat
Neni Oktiyani
Medical Laboratory Technology Department, Poltekkes Kemenkes Banjarmasin, Indonesia
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

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PERBANDINGAN KADAR HEMOGLOBIN ANTARA ANAK SD DI DATARAN RENDAH DAN TINGGI WILAYAH KERJA PUSKESMAS DIRGAHAYU KOTABARU TAHUN 2024 Rahmatus Sholehah; Neni Oktiyani; Ahmad Muhlisin; Wahdah Norsiah
Jurnal Karya Generasi Sehat Vol. 3 No. 2 (2025): Edisi Desember 2025
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v3i2.218

Abstract

Hemoglobin ialah unsur utama dalam sel darah merah yang tergolong sebagai protein terkonjugasi, berfungsi penting dalam proses pengangkutan oksigen serta karbon dioksida dalam tubuh. Hasil pemeriksaan terhadap sejumlah parameter darah seperti eritrosit, hemoglobin, dan hematokrit memperlihatkan adanya perbedaan yang mencolok antara individu yang tinggal di daerah dataran tinggi dengan mereka yang menetap di daerah dataran rendah. Kondisi kadar oksigen yang menurun seiring dengan peningkatan ketinggian menyebabkan tubuh beradaptasi dengan meningkatkan produksi sel darah merah demi mencukupi kebutuhan oksigen. Semakin tinggi lokasi tempat tinggal, semakin tinggi pula jumlah eritrosit yang diproduksi. Oleh karena itu, jumlah eritrosit memiliki kaitan erat dengan kadar hemoglobin dan hematokrit. Penelitian ini bertujuan guna mengetahui apakah ada perbedaan kadar hemoglobin antara siswa sekolah dasar yang berdomisili di dataran tinggi dan dataran rendah di wilayah kerja Puskesmas Dirgahayu, Kabupaten Kotabaru. Penelitian ini menggunakan desain observasional analitik dengan pendekatan cross sectional dan bersifat komparatif. Pemeriksaan dilakukan secara otomatis menggunakan alat hematology analyzer terhadap sampel darah vena dari 15 siswa di dataran rendah dan 15 siswa di dataran tinggi. Hasil analisis menggunakan uji Mann Whitney U menunjukkan nilai signifikansi sebesar 0,003, yang berarti ada perbedaan yang signifikan antara kedua kelompok. Rata-rata kadar hemoglobin pada siswa di dataran rendah tercatat sebesar 11,6 g/dl, sedangkan pada siswa di dataran tinggi mencapai 12,6 g/dl. Penelitian selanjutnya disarankan guna dilakukan pada wilayah dengan ketinggian 725 mdpl dengan melibatkan subjek remaja maupun orang dewasa.
PERBEDAAN KUALITAS PEWARNAAN SEDIAAN SITOLOGI MENGGUNAKAN BUNGA TELANG (Clitoria ternatea L) SEBAGAI ALTERNATIF PENGGANTI METHYLENE BLUE PADA METODE DIFF QUICK Rizky Arsyanti; Neni Oktiyani; Aima Insana; Ratih Dewi Dwiyanti
Jurnal Karya Generasi Sehat Vol. 3 No. 2 (2025): Edisi Desember 2025
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v3i2.232

Abstract

Pewarnaan sediaan sitologi dilakukan untuk memperjelas morfologi sel agar dapat diamati secara mikroskopis, khususnya pada bagian inti sel dan sitoplasma. Pewarnaan Diff Quick merupakan metode yang umum digunakan karena cepat dan memiliki kontras warna yang baik, tetapi salah satu komponennya, methylene blue, bersifat toksik dan tidak ramah lingkungan. Penelitian ini bertujuan untuk mengetahui perbedaan kualitas pewarnaan sediaan sitologi menggunakan larutan bunga telang (Clitoria ternatea L) sebagai alternatif alami pengganti methylene blue dengan pewarnaan Diff Quick. Jenis penelitian True Experiment dengan rancangan Post Test Only with Control Group Design, terdiri dari 4 perlakuan dengan 6 kali pengulangan menggunakan 1 sampel cairan pleura yang diwarnai dengan larutan bunga telang pada konsentrasi 20%, 25%, dan 30%, serta dibandingkan dengan pewarnaan Diff Quick standar sebagai kontrol. Hasil pemeriksaan mikroskopik larutan bunga telang konsentrasi 20% menunjukkan kualitas tidak baik, 25% menunjukkan kualitas cukup baik dan 30% menunjukkan kualitas pewarnaan yang paling mendekati pewarnaan standar dengan kategori “sangat baik.” Uji Friedman menunjukkan perbedaan yang signifikan antar kelompok perlakuan (p=0,001), dan uji Wilcoxon menunjukkan tidak terdapat perbedaan signifikan antara larutan bunga telang 30% dengan kontrol (p=0,063). Kesimpulannya, larutan bunga telang konsentrasi 30% memiliki potensi sebagai pewarna alami alternatif methylene blue dalam Metode Diff Quick. Disarankan untuk dilakukan penelitian lebih lanjut dengan menaikkan konsentrasi larutan pewarna bunga telang atau menaikkan pH larutan pewarna bunga telang (Clitoria ternatea L).
The Relationship between ABO Blood Type, Knowledge Level, Behavior, and Hypertension Status in Young Adults on Campus Neni Oktiyani; Tini Elyn Herlina; Ricko Dharmadi Utama
Medical Laboratory Technology Journal Vol. 12 No. 1 (2026): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v12i1.700

Abstract

Hypertension is a public health problem increasingly found among young adults, including college students. This study aims to analyze the relationship between ABO blood type, knowledge level, behavior, and hypertension status in young adults on campus. This study used an analytical survey method with a cross-sectional design. The study population consisted of all active students in the Medical Laboratory Technology Study Program at the Poltekkes Kemenkes Banjarmasin, with a sample size of 99 respondents selected using a total sampling technique. The researchers collected data through online questionnaires and blood pressure measurements. Data analysis used the Chi-Square test with a significance level of p<0.05. The results showed that the majority of respondents were female (80.8%), had normal blood pressure (67.7%), had good knowledge (69.7%), and had good behavior (58.6%). Statistical test results showed that gender (p=0.533), ABO blood type (p=0.916), and behavior (p=0.231) were not significantly associated with hypertension status. Conversely, knowledge level was significantly associated with hypertension status (p=0.044). This study concluded that knowledge level was associated with hypertension status, while ABO blood type and behavior showed no significant association. These findings highlight the importance of improving health literacy as part of hypertension prevention efforts in young adults.
Crackers Made from Nagara Bean (Vigna unguiculata L.) and Three-Spot Gourami (Trichopodus trichopterus) Flours as Complementary Food for Stunting Prevention Andrestian, Meilla Dwi; Dewi, Zulfiana; Oktiyani, Neni; Noraini, Noraini
Indonesian Food Science and Technology Journal Vol. 9 No. 2 (2026): Volume 9 Number 2, July 2026 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v9i2.47896

Abstract

Nagara beans (Vigna unguiculata L.) (NB) and three-spot gourami (Trichopodus trichopterus) (TS) can be used as blended flour, namely “Kanasawa” blended flour (KBF).  The KBF is developed into complementary food products, such as crackers, namely “Kanasawa Crackers”, to prevent stunting.  This study aimed to investigate the sensory quality, proximate and amino acids profile, Calcium and Phosphorus levels of “Kanasawa Crackers” from KBF.  This study is an experimental research that used a Complete Random Design with five formulas of proportion between KBF and mocaf flour, namely: C1 (0:100), C2 (25:75), C3 (50:50), C4 (75:25), C5 (100:0).  The best crackers formula was selected using de Garmo analysis.   The most preferred cracker product is C2.  The best Kanasawa crackers formula consisted of 7.81% water, 3.58% protein, 23.68% fat, 4.90% ash, 2.25% crude fibre, 52.09% carbohydrate, 18 types of amino acid, 0.52% Calcium, and 0.56% Phosphorus. “Kanasawa Crackers” with a KBF-to-mocaf flour ratio of 25:75 are suitable as a complementary food to prevent stunting.
Evaluation of Cinnamomum verum Bark Essential Oil as a Natural Antifungal Agent Against Dermatophytes Leka Lutpiatina; Annisa Aulia Damayanti; Akhmad Rhafianor; Neni Oktiyani; Aima Insana
Tropical Health and Medical Research Vol. 8 No. 2 (2026): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v8i2.156

Abstract

Dermatophytosis is a superficial fungal infection that remains a public health problem, particularly in tropical regions, with Trichophyton rubrum and Trichophyton mentagrophytes as the primary causes. Increasing limitations in the use of synthetic antifungals have encouraged the development of natural products as alternative therapies, including Cinnamomum verum stem bark essential oil. This study aimed to analyse the antifungal activity of C. verum stem bark essential oil against T. rubrum and T. mentagrophytes using the disc diffusion method. The study used a true experimental design with a posttest-only control group design. The essential oil was obtained through steam-water distillation with a yield of 0.25% (v/w). Tests against T. rubrum used concentrations of 1%, 2%, 5%, and 10%, while tests against T. mentagrophytes used concentrations of 5%, 10%, 15%, and 20%. Ketoconazole 2% was used as a positive control and dimethyl sulfoxide as a negative control. The inhibition zone diameter was measured after incubation and analysed using One-Way ANOVA and Post Hoc tests. The results showed that the essential oil inhibited the growth of both fungal species at all concentrations tested. The inhibition zone diameter against T. rubrum increased from 21.7 mm to 54.1 mm, while against T. mentagrophytes it increased from 33.6 mm to 48.2 mm. Statistical analysis showed a significant difference between the concentrations of both species (p<0.001). It was concluded that C. verum bark essential oil exhibited in vitro antifungal activity against both T. rubrum and T. mentagrophytes, with the inhibitory activity generally increasing with increasing concentrations. These findings demonstrate the potential of C. verum essential oil as a candidate for a natural antifungal agent based on local resources. Therefore, further research on the characterisation of active compounds, determination of MIC/MFC values, development of topical formulations, and in vivo testing and clinical trials are needed to support its use in dermatophytosis therapy.
Co-Authors Ahmad Luthfi Haqiqi Ahmad Muhlisin Ahmad Muhlisin Ahmad Muhlisin Aima Insana Akhmad Muhlisin Akhmad Rhafianor AMALIA PUTRI, NADIA Annisa Aulia Damayanti Annisa Miladdiannur Anny Thuraidah, Anny Ayu Sutiany Chairana Fadya Dewi, Zulfiana Erfan Roebiakto Erfan Roebiakto Erfan Roebiakto, Erfan fahriyan fahriyan Haitami Haitami haitami haitami, haitami Jujuk Anton Cahyono Jujuk Anton Cahyono Jujuk Anton Cahyono, Jujuk Anton Kusuma, Rio Jati Leka Lutpiatina Mahpolah Mahpolah Meilinda, Intan Meilla Dwi Andrestian, Meilla Dwi Monika Yati Noor Hikmah Damayanti Noraini Noraini, Noraini Nurhayati Aslami Alma Nurlailah Nurlailah Nurlailah Nurlailah NURUL HIDAYAH Prasetyastuti Prasetyastuti Rahmatus Sholehah Rahmawati Rahmawati Rahmawati Ratih Dewi Dwiyanti Ricko Dharmadi Utama Rif'ah, Puteri Amalia Rifqoh Rifqoh Rizky Arsyanti Ruri Widyati Sunarti Sunarti Sunarti, Sunarti Sutiany, Ayu Tini Elyn Herlina Tony Adi Setiawan Tri Setyawati Ummu Salamah Az Zahra Wahdah Norsiah wahdah norsiah Wahdah Norsiah, Wahdah Widyati, Ruri Yanti, Marini Aprida Yayuk Kustiningsih yayuk kustiningsih, yayuk