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All Journal Jurnal Hortikultura Indonesia (JHI) METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana SIMBIOSIS Biota Jurnal Mikologi Indonesia Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) BIO-CONS: Jurnal Biologi dan Konservasi BIO-CONS: Jurnal Biologi dan Konservasi BIOSAPPHIRE: Jurnal Biologi dan Diversitas
Made Ria Defiani
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Other

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Journal : Biota

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Pollen Viability and Microspore Culture in Three Broccoli Cultivars (Brassica oleracea L. var. italica Plenck) Septarini Dian Anitasari; Ida Ayu Astarini; Made Ria Defiani; Made Pharmawati .; Dian Catur Prayantini
Biota Vol 5 No 2 (2019): Jurnal Biota 2019
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v5i2.3340

Abstract

Broccoli is a high value vegetable crop in Indonesia, however production is low due to limited number of suitable cultivars, so, breeding hybrid broccoli for warm climate is important. The first step in hybridization is providing homozygote parent plants which can be done efficiently via microspore culture. The objectives of this study were to determine 1) bud size that produce uninucleate microspore stage appropriate for culture; 2) pollen viability, 3) microspore development, in three broccoli cultivars (‘BL 10001’, ‘Royal Green’ and ‘Green Magic’). Various bud size (1 – 5 cm) was squashed and observed microscopically to determine bud size containing uninucleate microspore. Pollen viability was determined by IKI staining and pollen germination method. Chromosome number was counted on root tips using squash method with aceto-orcein stain. Various heat treatment schemes were conducted to induce microspreo development. Result showed uninucleate microspore derived from 2-3 mm and 3-4 mm bud length of ‘BL-10001’ and ‘Royal Green’ was responsive for microspore development in culture. Pollen viability varied among cultivars, 78-87% on IKI method and 15-16% on germination test. Microspore culture showed different embryogenesis response; pollen-like structure was produced by ‘BL 10001’.
Co-Authors A A Ayu Laksmi Damarnegari A.A.S. A. Sukmaningsih Anitasari, Septarini Dian Dian Catur Prayantini Dwi Nur Rikhma Sari Eniek Kristiyanti ENIEK KRISWIYANTI Hikmah Putri Wahyu Utami I KETUT MUKSIN I Made Anom Sutrisna Wijaya I Made Saka Wijaya I.M.A.S. Wijaya I.M.S. Wijaya IDA AYU ASTARINI Ida Bagus Gede Darmayasa Ida Bagus Wayan Gunam Lily Windasari Luh Made Ary Somia Vagestini Luh Putu Melandani Luh Putu Wrasiati MADE PHARMAWATI MADE SUSUN PARWANAYONI Meitini Proborini Wahyuni Mia Kosmiatin Ni Kadek Raleni Ni LUH ARPIWI Ni Luh Putu Kayika Febryanti Ni Luh Suriani Ni Putu Adriani Astiti Ni Putu Harta Diani Pande Ni Putu Yuliatiningsih Novita Sari Puji Wahyu Lestari Putu Wina Andriani Lestari Rachel Daniella Dinda Maria Lumban Tobing RANI RACHMAWATI Retno Kawuri Septarini Dian Anitasari Septarini Dian Anitasari Septarini Dian Anitasari Tadzkia Hanifah Akbar Wira Purnama Santi Yully Muharyati