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All Journal Jurnal Sain Veteriner Jurnal Veteriner Jurnal Sain Peternakan Indonesia Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Ekonomi Pembangunan Wahana Peternakan Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) Jurnal Kedokteran Hewan Jurnal Media Tropika: Jurnal Pengabdian Kepada Masyarakat Buletin Pengabdian Jurnal Pengabdian Fakultas Pertanian Universitas Lampung
Ratna Ermawati
Study Program Of Animal Husbandry, Department Of Animal Husbandry, Faculty Of Agriculture, University Of Lampung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : Jurnal Veteriner

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Penerapan Metode Diagnosis Cepat Virus Avian Influenza H5N1 dengan Metode Single Step Multiplex RT-PCR Aris Haryanto; Ratna Ermawati; Medania Purwaningrum; Dini Wahyu Yudianingtyas; Michael Haryadi Wibowo; Charles Rangga Tabbu
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Avian influenza (AI) virus is a segmented single stranded (ss) RNA virus with negative polarity andbelong to the Orthomyxoviridae family. Diagnose of AI virus can be performed using conventional methodsbut it has low sensitivity and specificity. The objective of the research was to apply rapid, precise, andaccurate diagnostic method for AI virus and also to determine its type and subtype based on the SingleStep Multiplex Reverse Transcriptase-Polymerase Chain Reaction targeting M, H5, and N1 genes. In thismethod M, H5 and NI genes were simultaneously amplified in one PCR tube. The steps of this researchconsist of collecting viral RNAs from 10 different AI samples originated from Maros Disease InvestigationCenter during 2007. DNA Amplification was conducted by Simplex RT-PCR using M primer set. Then, bysingle step multiplex RT-PCR were conducted simultaneously using M, H5 and N1 primers set. The RTPCRproducts were then separated on 1.5% agarose gel, stained by ethidum bromide and visualized underUV transilluminator. Results showed that 8 of 10 RNA virus samples could be amplified by Simplex RTPCRfor M gene which generating a DNA fragment of 276 bp. Amplification using multiplex RT-PCRmethod showed two of 10 samples were AI positive using multiplex RT-PCR, three DNA fragments weregenerated consisting of 276 bp for M gene, 189 bp for H5 gene, and 131 bp for N1. In this study, rapid andeffective diagnosis method for AI virus can be conducted by using simultaneous Single Step Multiplex RTPCR.By this technique type and subtype of AI virus, can also be determined, especially H5N1.
Co-Authors Abimanyu Prastyo Ardiansyah Adek Rayhan Regisa Afrianti, Zulvina Aksal Alfarizki Alfarizki, Aksal Alfaruq, Fahmi Fadhilah Ali Husni Ardiansyah, Abimanyu Prastyo Arif Qisthon Aris Haryanto Ayu Fitriani Ayu Lidyana Ayu Lidyana Az-Zahra, Shabrina Bayu Hadi Setya Irawan Budiyanto, Ferly Rahmat Charles Rangga Tabbu Cici Hardiyanti Dakhlan, Akhmad Deny Sapto Chondro Utomo Desmawan, Deris Dhani, Akbar Rahmat Dian Septinova Dini Wahyu Yudianingtyas Emi Maimunah Erwanto Erwanto Erwanto Fajar Shodiq Permata Fani Setiawan Farida Fathul Fatmawati, Siti Tika Fauzi, Teo Achmad Fina Arzakiyah Fitria Tsani Farda Fitria Tsani Farda Guntur Januar Yudhistira Hanafi, Riyan Hanip Rangga Saputra Hardiyanti, Cici Hari, Putu Gaura Hermilinda Parera Hizkia, Ezra I Kadek Dwi Agus Candra Wijaya Ilhamsyah, Alifudin Imam Widodo Imam Widodo Imam Widodo Irawan, Bayu Hadi Setya Juandita, Karina Natasya Karina Natasya Juandita Kristanti, Amelia Evi Kusuma, Yosea Talenta Lidyana, Ayu Liman Liman Liman Liman Liman, Liman Madi Hartono Madi Hartono Madi Hartono Madi Hartono Madi Hartono Mahfud Rivai Medania Purwaningrum Mia Widowati Michael Haryadi Wibowo Muhammad Mirandy Pratama Sirat Muhammad Mirandy Pratama Sirat Muhiddin Sirat Muhtarudin Muhtarudin mulya, arif eka Nisak, Rohmatin Nur Aini Nur Aini Pramana, Sandika Purnama Edy Santosa Purnama Edy Santosa Purnama Edy Santosa Purnama Edy Santosa Purnama Edy Santosa Purnama Edy Santosa Purnama Edy Santosa Purnama Edy Santosa Rais, Ambarwati Mirna Ramadanti, Andin Hairunnisa Ramadhani, Afiqah Regisa, Adek Rayhan Ririn Angriani Rivai, Mahfud Riyan Hanafi Riyan Hanafi Rohmatin Nisak Rr Riyanti, Rr S Siswanto Safira, Auliya Sagala, Yollanda Natalia Sandika Pramana Sani, Darwan Sanjaya, Purba Saputra, Hanip Rangga Setio, Slamet Shaleh, Shofyan Sherlina Widya Rahma Sherlina Widya Rahma Sirat, Muhiddin Siswanto Siswanto Siswanto Siswanto Siswanto Siswanto Siti Tika Fatmawati Siti Tika Fatmawati Slamet Setio Sri Suharyati Surmini Surmini Surmini Surmini Surmini, Surmini Syahrio Tantalo Syahrio Tantalo Syahrio Tantalo Teo Achmad Fauzan Teo Achmad Fauzi Teo Achmad Fauzi Utami Syifana Widyastuti Veronica Wanniatie Widowati, Mia Yollanda Natalia Sagala Yudhistira, Guntur Januar Yuliana, Darma Zulvina Afrianti