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Andarini Diharmi
Departemen Teknilogi Hasil Perikanan Fakultas Perikanan Dan Kelautan Universitas Riau
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Pengaruh Suhu Pemanasan terhadap Karakteristik Garam Fungsional dari Rumput Laut Cokelat (Sargassum aquifolium) Silaban, Deni Saputra; Sukmiwati, Mery; Diharmi, Andarini
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 1 (2024): Juni 2024
Publisher : Politeknik - Ahli Usaha Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v19i1.966

Abstract

Sargassum aquifolium adalah rumput laut cokelat berpotensi sebagai bahan baku garam fungsional. Penelitian ini bertujuan untuk menentukan pengaruh suhu pemanasan berbeda terhadap karakteristik garam fungsional S. aquifolium. Metode yang digunakan adalah eksperimen menggunakan Rancangan Acak Lengkap (RAL) non faktorial. Perlakuan terdiri atas suhu pemanasan berbeda 25°C, 30°C, dan 35°C dengan 3 kali pengulangan. Parameter analisis terdiri atas rendemen, kadar natrium, kalium, NaCl, komponen bioaktif, dan aktivitas antioksidan. Rendemen garam S. aquifolium yang dihasilkan dengan proses diektraksi pada suhu 25°C, 30°C, dan 35°C berturut-turut sebesar 3,73%; 5,93%; dan 8,01%. Kadar natrium dan kalium garam S. aquifolium pada pemanasan dengan suhu 25°C, 30°C dan 35°C berturut-turut adalah; 1,92 mg/g; 2,29 mg/g dan 2,55 mg/g (Na), dan 39,18 mg/g; 42,64 mg/g dan 46,00 mg/g (K). Kadar NaCl yang dihasilkan dengan suhu pemanasan (25, 30, dan 25°C) sebesar 13,43%; 10,37% dan 7,95%. Senyawa bioaktif garam S. aquifolium terdiri atas fenolik, flavonoid, steroid dan saponin. Aktivitas antioksidan pada garam S. aquifolium dengan pemanasan suhu 25°C, 30°C dan 35°C diperoleh nilai IC50 berturut-turut 139,10 ppm, 147,34 ppm, dan 194.40 ppm. Perlakuan suhu pemanasan terbaik pada proses ektraksi S. aquifolium adalah 35°C, garam yang dihasilkan memiliki karakteristik rendemen 8,01%, kadar natrium 2,55 mg/g, kalium 46,00 mg/g, NaCl 7,95%. Senyawa bioaktif yang diperoleh terdiri dari fenolik, flavonoid, steroid, dan saponin. Aktivitas antioksidan garam S. aquifolium dengan nilai IC50 sebesar 194,40 ppm.AbstractSargassum aquifolium is a brown seaweed that has potential as a raw material for functional salt. This research aimed to determine the effect of different heating temperatures on the functional salt characteristics of S. aquifolium. The method used was an experiment using a non-factorial Completely Randomized Design (CRD). The treatment consisted of different heating temperatures of 25°C, 30°C, and 35°C with 3 repetitions. The analysis parameters consist of yield, content of sodium, potassium, NaCl, bioactive compound, and antioxidant activity. The yield of S. aquifolium salt produced by the extraction process at temperatures of 25°C, 30°C, and 35°C was 3.73%; 5.93%; and 8.01%. The sodium and potassium content of S. aquifolium salt when heated to a temperature of 25°C, 30°C, and 35°C were; 1.92 mg/g; 2.29 mg/g, and 2.55 mg/g (Na) and 39.18 mg/g; 42.64 mg/g and 46.00 mg/g (K). The NaCl content produced by heating temperatures (25, 30, and 25C) was 13.43%; 10.37%, and 7.95%. The bioactive compounds of S. aquifolium salt consist of phenolics, flavonoids, steroids, and saponins. The antioxidant activity of S. aquifolium salt by heating at 25°C, 30°C, and 35°C obtained IC50 values of 139.10 ppm, 147.34 ppm, and 194.40 ppm respectively. The best heating temperature treatment in the S. aquifolium extraction process was 35°C. The resulting salt has a yield characteristic of 8.01%, sodium content 2.55 mg/g, potassium 46.00 mg/g, and NaCl 7.95%. The bioactive compounds are phenolics, flavonoids, steroids, and saponins. The antioxidant activity of S. aquifolium salt has an IC50 value of 194.40 ppm.Keywords: antioxidant, functional salt, sodium, NaCl, potasium, Sargassum aquifolium
CHARACTERISTICS OF CARAGENAN FROM RED SEAWEED (RHODOPHYTA) Eucheuma spinosum AND Eucheuma cottonii ORIGINATING FROM MORO ISLAND Siregar, Ika Darmila; Diharmi, Andarini; Karnila, Rahman
Asian Journal of Aquatic Sciences Vol. 8 No. 3 (2025): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.8.3.410-418

Abstract

Red seaweeds Eucheuma spinosum and E. cottonii are raw materials for producing carrageenan. Carrageenan is a group of polysaccharides widely used as thickening, stabilizing, and gelling agents across various industries. This study aims to determine the physicochemical characteristics of carrageenan from E. spinosum and E.cottonii seaweed. This research uses an experimental method: direct extraction with hot alkali, with three repetitions. The physicochemical parameters analyzed include yield, gel strength, viscosity, water content, ash content, and sulfate content. The results of the study of the physicochemical characteristics of carrageenan from E. spinosum raw materials produced a yield of 34.41%, gel strength 22.17 g/cm2, viscosity 1450 cP, water content 11.57%, ash content 24.41%, and sulfate content 24.89%. The results of the study of the physico-chemical characteristics of E. cottonii produced a yield of 29.54%, gel strength of 488.58 g/cm2, viscosity of 30.05 cP, water content of 9.95%, ash of 36.42%, and sulfate content of 21.42%. The FTIR profiles of E. spinosum carrageenan compounds show iota carrageenan, and those of E. cottonii carrageenan compounds show kappa carrageenan. The physicochemical characteristics of E. spinosum and E.cottonii carrageenan raw materials have met FAO standards, except for gel strength and ash content in E. cottonii carrageenan.
Anticancer activity of Stichopus vastus extract against MCF-7 and HeLa cells from Natuna waters Sukmiwati, Mery; Nurjanah, Nurjanah; Diharmi, Andarini; Putri, Indah Prasetya
JURNAL ILMU KEFARMASIAN INDONESIA Vol. 23 No. 1 (2025): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v23i1.1631

Abstract

Sea cucumbers (Stichopus vastus) contain bioactive compounds with potential anticancer properties, making them a promising alternative raw material for pharmaceutical development. This study aimed to isolate and fractionate bioactive compounds from S. vastus and evaluate their anticancer activity against MCF-7 and HeLa cell lines. Isolation was performed using Thin Layer Chromatography (TLC), and cytotoxic activity was assessed in vitro using the MTT assay. Phytochemical screening revealed the presence of steroids, terpenoids, and saponins. IC₅₀ values were calculated through linear regression analysis. The methanol extract exhibited IC₅₀ values of 13.50 µg/mL (HeLa) and 17.25 µg/mL (MCF-7), indicating active anticancer potential. The n-hexane fraction showed IC₅₀ values of 10.09 µg/mL (HeLa) and 15.11 µg/mL (MCF-7), also categorized as active. Notably, the ethyl acetate fraction demonstrated superior activity, with an IC₅₀ of 7.00 µg/mL against HeLa (very active) and 13.32 µg/mL against MCF-7 (active). These findings suggest that the ethyl acetate fraction of S. vastus holds significant promise as an anticancer agent, particularly against HeLa cells.
Characteristics of catfish oil, red palm oil and shark liver oil as functional foods Amri, Ulil; Diharmi, Andarini; Sukmiwati, Mery
Depik Vol 10, No 2 (2021): August 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.2.19131

Abstract

Functional food is a food ingredient in addition to basic needs as nutrients that can also play a functional role in health. This research aimed to determine the physicochemical characteristics and fatty acid composition of catfish oil, red palm oil, and shark liver oil as functional food ingredients. The research method was to extract fish oil from belly flap, purify catfish oil, and process red palm oil (RPO) from crude palm oil (CPO). The analysis parameters consisted of sensory analysis, oil chemical characteristics (free fatty acid analysis, peroxide, iodine, saponification, and acid numbers), total carotene, tocopherol, and analysis of fatty acid composition. The results showed that the catfish oil after being purified had sensory characteristics, smelled slightly fishy and semi-solid, and had a bright yellow color. The results of the analysis of chemical characteristics showed that the free fatty acid numbers of catfish oil and shark liver oil were following IFOS standards (1.33 and 0.62%), and the RPO numbers for peroxide and free fatty acids according to the SNI standards (9.56 meq kg and 1.44%). The highest -3 and - 6 fatty acids were in shark liver oil (3.56 and 35.35%), followed by catfish oil (1.72 and 19.9%). and RPO does not contain -3 and -6. Catfish oil, RPO, and shark liver oil act as functional foods. The fatty acid composition of catfish, shark liver and red palm oil contains saturated and the fatty acid composition of catfish, shark liver and red palm oil contains saturated and unsaturated fatty acids. Mono and poly unsaturated fatty acid (FUFA anf MUFA) in crude catfish oil, pure catfish oil, shark liver oil, and red palm oils were 56.71, 58.12, 63.81 and 47.39% respectively. The result of analysis showed composition of in catfish oil 1.72 and 19.9 %. The content of and of shark liver oil was 3.5 and 35.5%. Whereas in red palm oil does not 3 and 6. The content of EPA and DHA in shark liver oil was 0.08, 0.09 but not in catfish and red palm oil. The total content of carotene and tocopherol in red palm oil was 513.86 and 925.80 mg/kg, respectively. The nutritional composition of catfish oil, red palm oil, and shark liver oil has the potential to be used as functional food.Keywords:Characteristic physicochemicalCaroteneTocopherol-3-6
Aktivitas Antioksidan Hidrolisat Protein Ikan Cunang (Congresox talabon) Anggi Angelita Hermaya; Edison Edison; Andarini Diharmi
JURNAL AGROINDUSTRI HALAL Vol. 7 No. 1 (2021): Jurnal Agroindustri Halal 7(1)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jah.v7i1.3593

Abstract

Cunang fish (Congresox talabon) has the potential as a raw material for fish protein hydrolyzates. Hydrolysis of cunang fish using the enzyme papain were produced peptides and amino acids that have antioxidant activity. The aim of this study was to determine the antioxidant activity of the protein hydrolyzate of cunang fish using different papain enzyme concentrations. This study used an experimental method with a completely randomized non-factorial design with three replications, with different papain enzyme concentrations (2%, 4%, 6%). The analysis parameters consisted of proximate content analysis in fish meat, degree of hydrolysis, the dissolved protein of the Bradford method, and the antioxidant activity of the DPPH method on the protein hydrolysate.  The results showed that the protein content of cunang fish had 73.15% (bk). The papain enzyme concentration of 6% was the best concentration hydrolyze cunang meat, is produced. protein hydrolysate.  The results of the protein hydrolyzate analysis showed that the degree of hydrolysis was 46.23%, the dissolved protein was 11.64% and the antioxidant activity was 549.16 ppm. Enzyme hydrolysis can increase dissolved protein content which is directly proportional to the increase in antioxidant activity.
Co-Authors Agus Syahroni Agusman, Indra Akhyar Ali Alghifary, Zaki Allecya Tri Elisabeth Sihombing Anggi Angelita Hermay Anggi Angelita Hermaya Azmi, Heriza Br. Sianturi1, Frisdauli junitaMargaret Chicka Willy Yanti Dahlia Dahlia Deando Hentun Gultom Dedi Fardiaz Deviasari Deviasari Dewi Fortuna Ayu Dewita Dewita Dewita, Dewitad Dian Iriani Edison Edison Edison Edison Edison Edison Edison Edison Edison Edison Edison Edison Ela Davera Sari Enda Mora Endang Sri Heruwati Endang Sri Heruwati Fachrul Roji Fatma Eka Putri Febriani Melisa Sinurat Febriani Melisa Sinurat Febrina Tumangger Febrina Tumangger Feby Dwi Anisha Putri G. Gunawan Helmi Nurhanif Henni Syawal Herawati Br Ginting Heriyanti Heriyanti, Heriyanti Hermaya, Anggi Angelita Hotmeriah Padang Illahi, Sikarina Ilman Arif Indah Azisari Indah Azisari Indra Agusman Indra Agusman Ira Sari Irasari, N. Irene Charistia Jhosua Fransiskus Karnila, Rahman Karnila Kenzo Aditya Muhammad Yandhria Putra Kristina Natalia Kusai Kusai Kusai, Kusai Lamun Bathara Lorde Sembiring Lorde Sembiring M Lamudin Noor M. Faiz Akhimuddin M. Ufan M. Ufan M.Faisal Saputra S M.Halim M.Halim Manurung, Rahel Eriani Maria Marifa Indar Wara Gandini Mery Sukmiwati Mirna Ilza Mirna Ilza Mirna Ilza Muhammad Arief Muhammad Zakiyul Fikri N Ira Sari N Ira Sari N Ira Sari N Ira Sari N Ira Sari N. Ira Sari N. Nurjanah ndang Sri Heruwati Nofri Sandria Nola Rahma Aulia Noor Ira Sari Nur Ira Sari Nuraini Nuraini Nurhanif, Helmi Nuri Andarwulan Nurul Muji Ariani Oktavianti, Mila Panji Yaversio Praja Prayogo Priady Simatupang Putra, Sanjaya Mandala Putri Rahmadani Putri, Fatma Eka Putri, Indah Prasetya PUTRIANA SARI SIRAIT Rana Salma Reza Saputra Rezky Fransiskus Simanjuntak Rindi Metalisa, Rindi Ronapadua Musyirna Rahmah Nasution Sahara dan Emma Susanti Rossi, Evy RR. Ella Evrita Hestiandari Rusnawati Rusnawati Salma, Putri Santhy Wisuda Sidauruk Santika, Jesi Sari, Noor Ira Selamat ariyadin Sidauruk, Santhy W. Silaban, Deni Saputra Silfi Zuhriani Siregar, Cici YP Siregar, Ika Darmila Siregar, Rizky Febriansyah Siti Aminah Siti Khaizatul A’mal Suardi Loekman Sukendi Sukendi Sulistiana Pramita Nosa Sumarto Sumarto Sumarto Suparmi Suparmi Suparmi Syahada, Melania Syahrul Syahrul Syahrul Syahrul Syahrul Syahrul Syahrul Syahrul Tessha Fitriani Tiara Kurnia Sari Tiara Septiani Lumban Gaol Tince Sofyani Tince Sofyani Titieu Keumala Sukandar Tomi Ramadona Tri Ayu Ningsih Trisla Warningsih Ulil Amri Wahyudi Wahyudi Wikky Aditiya Putra Yenita Roza Yuni Afrida Yuni Suseno