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Characteristic of gelatin bone tilapia (Oreochromis nilaticus) extracted with acid and base Diharmi, Andarini; Nurhanif, Helmi; Sari, N Ira
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Jurnal Ilmu Perairan, Vol. 9: No. 2 (August, 2022)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v9i2.6159

Abstract

This study was aimed to determine chemical and physical characteristics of extracted tilapia fish bone gelatin using acid (HCl) and alkaline (NaOH) solutions. The research method was used comparative experimental with Paired t-test consisting of 2 treatments, namely 4% HCl and 4% NaOH with 3 replications. Analysis parameters was consisted of yield, gel strength, viscosity, pH, moisture content, and ash. Characteristics of gelatin extracted using acid solution was resulted in yield, gel strength and viscosity was 4.40, 182.65 g/bloom. and 9.90 cP. Characteristic of gelatin extracted with acid has moisture, ash and pH levels of 5.85%, 2.35%, and 4.25. Gelatin extracted with bases was produced physical characteristics of yield, gel strength, and viscosity of 4.67%, 150.41 g/bloom and 6.03 cP and chemical characteristics (moisture, ash, and pH) were 5.77%, 3.37%, and 10.24. The extraction process using acids and bases resulted in tilapia fish bone gelatin with significantly different characteristics on gel strength, viscosity, pH, and ash except yield and moisture content. Gelatin was produced from tilapia fish bones using acids has gel strength, pH, ash, and moisture content in accordance with SNI standards except for viscosity, while gelatin uses bases had gel strength, viscosity and moisture content, except for pH and ash.Keywords: characteristic physico-chemical; gel strength; pH; viscosity; yield
Identification of bioactive compounds in extract fraction red seaweed (Eucheuma cottonii) Agusman, Indra; Diharmi, Andarini; Sari, N Ira
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Jurnal Ilmu Perairan, Vol. 9: No. 2 (August, 2022)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v9i2.6164

Abstract

Seaweed is a biological resource that is rich in nutrients and bioactive compounds. This study aims to determine qualitatively and quantitatively the bioactive compounds (flavonoids, alkaloids, steroids/terpenoids, saponins and phenols) from extracts and fractions of Eucheuma cottonii. The method used is an experiment by conducting a series of experiments by extracting and fractionating E. cottonii and the data were analyzed descriptively. The treatments used were organic solvents with different polarity levels (methanol, n-hexane, ethyl acetate, and butanol) for extraction and fractionation. Parameter analysis consisted of qualitative phytochemical tests (flavonoids, alkaloids, steroids/terpenoids, saponins and phenols) and quantitatively determined the total content of alkaloids, flavonoids, and saponins. The results of the phytochemical test of methanol extract qualitatively produced alkaloids, flavonoids, steroids/terpenoids, and saponins. The n-hexane fraction contains steroids/terpenoids and saponins. The ethyl acetate fraction contains alkaloids, flavonoids and steroids/terpenoids and the butanol fraction contains alkaloids. The results of quantitative analysis of the n-hexane extract fraction showed that the content of saponins was 0.23%, alkaloids and flavonoids were 0.16% and 0.09% in the ethyl acetate fraction, while the alkaloid content was 0.09% in the butanol fraction.Keywords: fractionation; organic solvent; phytocemical; quantitative
Pengaruh Suhu Pemanasan terhadap Karakteristik Garam Fungsional dari Rumput Laut Cokelat (Sargassum aquifolium) Silaban, Deni Saputra; Sukmiwati, Mery; Diharmi, Andarini
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 1 (2024): Juni 2024
Publisher : Politeknik - Ahli Usaha Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v19i1.966

Abstract

Sargassum aquifolium adalah rumput laut cokelat berpotensi sebagai bahan baku garam fungsional. Penelitian ini bertujuan untuk menentukan pengaruh suhu pemanasan berbeda terhadap karakteristik garam fungsional S. aquifolium. Metode yang digunakan adalah eksperimen menggunakan Rancangan Acak Lengkap (RAL) non faktorial. Perlakuan terdiri atas suhu pemanasan berbeda 25°C, 30°C, dan 35°C dengan 3 kali pengulangan. Parameter analisis terdiri atas rendemen, kadar natrium, kalium, NaCl, komponen bioaktif, dan aktivitas antioksidan. Rendemen garam S. aquifolium yang dihasilkan dengan proses diektraksi pada suhu 25°C, 30°C, dan 35°C berturut-turut sebesar 3,73%; 5,93%; dan 8,01%. Kadar natrium dan kalium garam S. aquifolium pada pemanasan dengan suhu 25°C, 30°C dan 35°C berturut-turut adalah; 1,92 mg/g; 2,29 mg/g dan 2,55 mg/g (Na), dan 39,18 mg/g; 42,64 mg/g dan 46,00 mg/g (K). Kadar NaCl yang dihasilkan dengan suhu pemanasan (25, 30, dan 25°C) sebesar 13,43%; 10,37% dan 7,95%. Senyawa bioaktif garam S. aquifolium terdiri atas fenolik, flavonoid, steroid dan saponin. Aktivitas antioksidan pada garam S. aquifolium dengan pemanasan suhu 25°C, 30°C dan 35°C diperoleh nilai IC50 berturut-turut 139,10 ppm, 147,34 ppm, dan 194.40 ppm. Perlakuan suhu pemanasan terbaik pada proses ektraksi S. aquifolium adalah 35°C, garam yang dihasilkan memiliki karakteristik rendemen 8,01%, kadar natrium 2,55 mg/g, kalium 46,00 mg/g, NaCl 7,95%. Senyawa bioaktif yang diperoleh terdiri dari fenolik, flavonoid, steroid, dan saponin. Aktivitas antioksidan garam S. aquifolium dengan nilai IC50 sebesar 194,40 ppm.AbstractSargassum aquifolium is a brown seaweed that has potential as a raw material for functional salt. This research aimed to determine the effect of different heating temperatures on the functional salt characteristics of S. aquifolium. The method used was an experiment using a non-factorial Completely Randomized Design (CRD). The treatment consisted of different heating temperatures of 25°C, 30°C, and 35°C with 3 repetitions. The analysis parameters consist of yield, content of sodium, potassium, NaCl, bioactive compound, and antioxidant activity. The yield of S. aquifolium salt produced by the extraction process at temperatures of 25°C, 30°C, and 35°C was 3.73%; 5.93%; and 8.01%. The sodium and potassium content of S. aquifolium salt when heated to a temperature of 25°C, 30°C, and 35°C were; 1.92 mg/g; 2.29 mg/g, and 2.55 mg/g (Na) and 39.18 mg/g; 42.64 mg/g and 46.00 mg/g (K). The NaCl content produced by heating temperatures (25, 30, and 25C) was 13.43%; 10.37%, and 7.95%. The bioactive compounds of S. aquifolium salt consist of phenolics, flavonoids, steroids, and saponins. The antioxidant activity of S. aquifolium salt by heating at 25°C, 30°C, and 35°C obtained IC50 values of 139.10 ppm, 147.34 ppm, and 194.40 ppm respectively. The best heating temperature treatment in the S. aquifolium extraction process was 35°C. The resulting salt has a yield characteristic of 8.01%, sodium content 2.55 mg/g, potassium 46.00 mg/g, and NaCl 7.95%. The bioactive compounds are phenolics, flavonoids, steroids, and saponins. The antioxidant activity of S. aquifolium salt has an IC50 value of 194.40 ppm.Keywords: antioxidant, functional salt, sodium, NaCl, potasium, Sargassum aquifolium
Co-Authors Agus Syahroni Agusman, Indra Akhyar Ali Alghifary, Zaki Allecya Tri Elisabeth Sihombing Anggi Angelita Hermay Anggi Angelita Hermaya Azmi, Heriza Br. Sianturi1, Frisdauli junitaMargaret Chicka Willy Yanti Dahlia Dahlia Deando Hentun Gultom Dedi Fardiaz Deviasari Deviasari Dewi Fortuna Ayu Dewita Dewita Dewita, Dewitad Dian Iriani Edison Edison Edison Edison Edison Edison Edison Edison Edison Edison Edison Edison Ela Davera Sari Emma Susanti Enda Mora Endang Sri Heruwati Endang Sri Heruwati Fachrul Roji Fatma Eka Putri Febriani Melisa Sinurat Febriani Melisa Sinurat Febrina Tumangger Febrina Tumangger Feby Dwi Anisha Putri Helmi Nurhanif Henni Syawal Herawati Br Ginting Heriyanti Heriyanti, Heriyanti Hermaya, Anggi Angelita Hotmeriah Padang Illahi, Sikarina Ilman Arif Indah Azisari Indah Azisari Indra Agusman Indra Agusman Ira Sari Irasari, N. Irene Charistia Jhosua Fransiskus Karnila, Rahman Karnila Kenzo Aditya Muhammad Yandhria Putra Kristina Natalia Kusai Kusai Kusai, Kusai Lamun Bathara Lorde Sembiring Lorde Sembiring M Lamudin Noor M. Faiz Akhimuddin M. Ufan M. Ufan M.Faisal Saputra S M.Halim M.Halim Manurung, Rahel Eriani Maria Marifa Indar Wara Gandini Mery Sukmiwati Mirna Ilza Mirna Ilza Mirna Ilza Muhammad Arief Muhammad Zakiyul Fikri N Ira Sari N Ira Sari N Ira Sari N Ira Sari N Ira Sari N. Ira Sari N. Nurjanah ndang Sri Heruwati Nofri Sandria Nola Rahma Aulia Noor Ira Sari Nur Ira Sari Nuraini Nuraini Nurhanif, Helmi Nuri Andarwulan Nurul Muji Ariani Oktavianti, Mila Panji Yaversio Praja Prayogo Priady Simatupang Putra, Sanjaya Mandala Putri Rahmadani Putri, Fatma Eka Putri, Indah Prasetya PUTRIANA SARI SIRAIT Rana Salma Reza Saputra Reza Saputra Rezky Fransiskus Simanjuntak Rindi Metalisa, Rindi Rossi, Evy RR. Ella Evrita Hestiandari Rusnawati Rusnawati Salma, Putri Santhy Wisuda Sidauruk Santika, Jesi Sari, Noor Ira Selamat ariyadin Sidauruk, Santhy W. Silaban, Deni Saputra Silfi Zuhriani Siregar, Cici YP Siregar, Rizky Febriansyah Siti Aminah Siti Khaizatul A’mal Suardi Loekman Sukendi Sukendi Sulistiana Pramita Nosa Sumarto Sumarto Sumarto Suparmi Suparmi Suparmi Syahada, Melania Syahrul Syahrul Syahrul Syahrul Syahrul Syahrul Syahrul Syahrul Tessha Fitriani Tiara Kurnia Sari Tiara Septiani Lumban Gaol Tince Sofyani Tince Sofyani Titieu Keumala Sukandar Tomi Ramadona Tri Ayu Ningsih Trisla Warningsih Ulil Amri Wahyudi Wahyudi Wikky Aditiya Putra Yenita Roza Yuni Afrida Yuni Suseno