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Andarini Diharmi
Departemen Teknilogi Hasil Perikanan Fakultas Perikanan Dan Kelautan Universitas Riau
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Aktivitas Antioksidan Hidrolisat Protein Ikan Cunang (Congresox talabon) Anggi Angelita Hermaya; Edison Edison; Andarini Diharmi
JURNAL AGROINDUSTRI HALAL Vol. 7 No. 1 (2021): Jurnal Agroindustri Halal 7(1)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jah.v7i1.3593

Abstract

Cunang fish (Congresox talabon) has the potential as a raw material for fish protein hydrolyzates. Hydrolysis of cunang fish using the enzyme papain were produced peptides and amino acids that have antioxidant activity. The aim of this study was to determine the antioxidant activity of the protein hydrolyzate of cunang fish using different papain enzyme concentrations. This study used an experimental method with a completely randomized non-factorial design with three replications, with different papain enzyme concentrations (2%, 4%, 6%). The analysis parameters consisted of proximate content analysis in fish meat, degree of hydrolysis, the dissolved protein of the Bradford method, and the antioxidant activity of the DPPH method on the protein hydrolysate.  The results showed that the protein content of cunang fish had 73.15% (bk). The papain enzyme concentration of 6% was the best concentration hydrolyze cunang meat, is produced. protein hydrolysate.  The results of the protein hydrolyzate analysis showed that the degree of hydrolysis was 46.23%, the dissolved protein was 11.64% and the antioxidant activity was 549.16 ppm. Enzyme hydrolysis can increase dissolved protein content which is directly proportional to the increase in antioxidant activity.
Characteristics of catfish oil, red palm oil and shark liver oil as functional foods Ulil Amri; Andarini Diharmi; Mery Sukmiwati
Depik Vol 10, No 2 (2021): August 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.2.19131

Abstract

Functional food is a food ingredient in addition to basic needs as nutrients that can also play a functional role in health. This research aimed to determine the physicochemical characteristics and fatty acid composition of catfish oil, red palm oil, and shark liver oil as functional food ingredients. The research method was to extract fish oil from belly flap, purify catfish oil, and process red palm oil (RPO) from crude palm oil (CPO). The analysis parameters consisted of sensory analysis, oil chemical characteristics (free fatty acid analysis, peroxide, iodine, saponification, and acid numbers), total carotene, tocopherol, and analysis of fatty acid composition. The results showed that the catfish oil after being purified had sensory characteristics, smelled slightly fishy and semi-solid, and had a bright yellow color. The results of the analysis of chemical characteristics showed that the free fatty acid numbers of catfish oil and shark liver oil were following IFOS standards (1.33 and 0.62%), and the RPO numbers for peroxide and free fatty acids according to the SNI standards (9.56 meq kg and 1.44%). The highest -3 and - 6 fatty acids were in shark liver oil (3.56 and 35.35%), followed by catfish oil (1.72 and 19.9%). and RPO does not contain -3 and -6. Catfish oil, RPO, and shark liver oil act as functional foods. The fatty acid composition of catfish, shark liver and red palm oil contains saturated and the fatty acid composition of catfish, shark liver and red palm oil contains saturated and unsaturated fatty acids. Mono and poly unsaturated fatty acid (FUFA anf MUFA) in crude catfish oil, pure catfish oil, shark liver oil, and red palm oils were 56.71, 58.12, 63.81 and 47.39% respectively. The result of analysis showed composition of in catfish oil 1.72 and 19.9 %. The content of and of shark liver oil was 3.5 and 35.5%. Whereas in red palm oil does not 3 and 6. The content of EPA and DHA in shark liver oil was 0.08, 0.09 but not in catfish and red palm oil. The total content of carotene and tocopherol in red palm oil was 513.86 and 925.80 mg/kg, respectively. The nutritional composition of catfish oil, red palm oil, and shark liver oil has the potential to be used as functional food.Keywords:Characteristic physicochemicalCaroteneTocopherol-3-6
Komposisi dan Identifikasi Senyawa Metabolit Sekunder pada Rumput Laut Merah (Gelidium sp) dengan Metode Maserasi Putri Salma; Edison Edison; Andarini Diharmi
Jurnal Teknologi dan Industri Pertanian Indonesia Vol 17, No 1 (2025): Vol. (17) No. 1, April 2025
Publisher : Agriculture Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/jtipi.v17i1.34796

Abstract

Seaweed is one of the main aquaculture commodities that has economic value and has anormous potential in Indonesian. However the use of seaweed, especially the Gelidium sp type, is still undertutilitized. This research aims to determine the chemical composition and secondary metabolite compounds contained in the red seaweed Gelidium sp. The method used was experimental with different solvents, namely metanol, ethyl acetate and n-hexane in the extraction process. The test parameter analysis carried out was proximate analysis and analysis of secondary metabolite compounds in the red seaweed Gelidium sp. The results of the research were that in the proximate analysis, the water content was 14.42% in the wet weight, the ash content was 8.94% in the dry weight, The fat content was 1.25% in the drg weight and the carbohydrate content was 58.12%. The high carbohydrate content in seaweed is related to the presence of crude fiber components contained. Analysis of secondary metabolite compounds showed the presence of alkaloids, steroids, saponins and phenolics in the methanol solvent; alkaloid and steroid content in ethyl acetate; and alkaloids and steroids in n-hexane solvent. Secondary metabolite compounds in red seaweed Gelidium sp. It can be used as an antioxidant, antibacterial or medicine in the health sector
Aktivitas Antioksidan dan Inhibitor Alfa Glukosidase dari Fraksi Berbeda Rumput Laut Cokelat (Sargassum sp.) Rahel Eriani Maria Manurung; Andarini Diharmi; Edison Edison
Jurnal Teknologi dan Industri Pertanian Indonesia Vol 17, No 1 (2025): Vol. (17) No. 1, April 2025
Publisher : Agriculture Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/jtipi.v17i1.33228

Abstract

Sargassum sp. is one of the brown seaweed that contains bioactive compounds, which have antioxidant and alpha glucosidase inhibitor activity. This research aimed to determine the rendemen, secondary metabolic, total phenolic, antioxidant, and alpha glucosidase inhibitor from the extract and fractions of Sargassum sp. The research method used was an experiment by extracting Sargassum sp with methanol and the extract were fractionated with different polarity solvents (n-hexane, ethyl acetate, and ethanol). Data were analyzed descriptively. The analysis parameters consisted of rendemen, secondary metabolic (qualitative), total phenolics, antioxidants (DPPH and FRAP), and alpha glucosidase inhibitors. The results showed that the rendemen of the n-hexane, ethyl acetate, and ethanol fractions were 4,15, 5,81, and 4,94%, respectively. The secondary metabolic analysis of methanol extract were alkaloids, steroids/terpenoids, saponins, and phenolic. The n-hexane fraction were steroids/terpenoids, saponins, and phenolic. The ethyl acetate fraction were alkaloids, steroids/terpenoids, saponins, and phenolic. The ethanol and water fractions contained only saponins. The total phenolic content of the methanol extract, n-hexane, ethyl acetate, and etanol fractions were 21161,03, 7854,46, 1626,89, and 80,17 ppm. Antioxidants in methanol extract, n-hexane, ethyl acetate, ethanol fractions using DPPH were 329,42, 466,25, 535,08, and 80,17 ppm. Antioxidants in methanol extract, n-hexane, ethyl acetate, and ethanol fractions using FRAP were 196,15, 294,88, 364,26, and 492,68 ppm. Alpha glucosidase inhibitor in n-hexane fraction with an IC50 value of 538110,9 ppm. Extract and fractions of Sargassum sp. with different polarity solvents contained different secondary metabolic. Saponins were found in all solvents, phenolics and steroids/terpenoids were found in methanol extract, n-hexane, and ethyl acetate fractions, alkaloids were found in methanol exract and ethyl acetate fraction. The n-hexane fraction showed the highest activity in total phenol, antioxidant, and alpha glucosidase inhibitor.
Co-Authors Agus Syahroni Agusman, Indra Akhyar Ali Alghifary, Zaki Allecya Tri Elisabeth Sihombing Anggi Angelita Hermay Anggi Angelita Hermaya Azmi, Heriza Br. Sianturi1, Frisdauli junitaMargaret Chicka Willy Yanti Dahlia Dahlia Deando Hentun Gultom Dedi Fardiaz Deviasari Deviasari Dewi Fortuna Ayu Dewita Dewita Dewita, Dewitad Dian Iriani Edison Edison Edison Edison Edison Edison Edison Edison Edison Edison Edison Edison Edison Edison Ela Davera Sari Enda Mora Endang Sri Heruwati Endang Sri Heruwati Evy Rossi Fachrul Roji Fatma Eka Putri Febriani Melisa Sinurat Febriani Melisa Sinurat Febrina Tumangger Febrina Tumangger Feby Dwi Anisha Putri Helmi Nurhanif Henni Syawal Herawati Br Ginting Heriyanti Heriyanti, Heriyanti Hermaya, Anggi Angelita Hotmeriah Padang Illahi, Sikarina Ilman Arif Indah Azisari Indah Azisari Indra Agusman Indra Agusman Ira Sari Irasari, N. Irene Charistia Jhosua Fransiskus Karnila, Rahman Karnila Kenzo Aditya Muhammad Yandhria Putra Kristina Natalia Kusai Kusai Kusai, Kusai Lamun Bathara Lorde Sembiring Lorde Sembiring M Lamudin Noor M. Faiz Akhimuddin M. Ufan M. Ufan M.Faisal Saputra S M.Halim M.Halim Marifa Indar Wara Gandini Mery Sukmiwati Mirna Ilza Mirna Ilza Mirna Ilza Muhammad Arief Muhammad Zakiyul Fikri Musyirna Rahmah Nst N Ira Sari N Ira Sari N Ira Sari N Ira Sari N Ira Sari N. Ira Sari N. Nurjanah ndang Sri Heruwati Nofri Sandria Nola Rahma Aulia Noor Ira Sari Nur Ira Sari Nuraini Nuraini Nurhanif, Helmi Nuri Andarwulan Nurul Muji Ariani Oktavianti, Mila Panji Yaversio Praja Prayogo Priady Simatupang Putra, Sanjaya Mandala Putri Rahmadani Putri Salma Putri, Fatma Eka Putri, Indah Prasetya PUTRIANA SARI SIRAIT Rahel Eriani Maria Manurung Rana Salma Reza Saputra Rezky Fransiskus Simanjuntak Rindi Metalisa, Rindi RR. Ella Evrita Hestiandari Rusnawati Rusnawati Santhy Wisuda Sidauruk Santika, Jesi Sari, Noor Ira Selamat ariyadin Sidauruk, Santhy W. Silaban, Deni Saputra Silfi Zuhriani Siregar, Cici YP Siregar, Rizky Febriansyah Siti Aminah Siti Khaizatul A’mal Suardi Loekman Sukendi Sukendi Sulistiana Pramita Nosa Sumarto Sumarto Sumarto Suparmi Suparmi Suparmi Syahada, Melania Syahrul Syahrul Syahrul Syahrul Syahrul Syahrul Syahrul Syahrul Tessha Fitriani Tiara Kurnia Sari Tiara Septiani Lumban Gaol Tince Sofyani Tince Sofyani Titieu Keumala Sukandar Tomi Ramadona Tri Ayu Ningsih Trisla Warningsih Ulil Amri Wahyudi Wahyudi Wikky Aditiya Putra Yenita Roza Yuni Afrida Yuni Suseno