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All Journal Buletin Peternakan Natural Science: Journal of Science and Technology Jurnal Ilmu Lingkungan Jurnal Natur Indonesia Microbiology Indonesia Jurnal Riset Kimia Jurnal Kimia dan Kemasan Jurnal Litbang Industri IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Jurnal Zarah Alotrop Jurnal Pendidikan dan Ilmu Kimia Sinkron : Jurnal dan Penelitian Teknik Informatika INTECOMS: Journal of Information Technology and Computer Science Jurnal Katalisator Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan METIK JURNAL Jusikom: Jurnal Sistem Informasi Ilmu Komputer Jurnal Analis Farmasi Jurnal Agrotropika Jurnal Kimia dan Kemasan JAF (Jurnal Analis Farmasi) Jurnal Litbang Industri Makara Journal of Science Data Sciences Indonesia (DSI) TAMIKA: Jurnal Tugas Akhir Manajemen Informatika & Komputerisasi Akuntansi Journal of Engineering and Science Application

Abdi Dharma

Universitas Andalas

Author-ID : 253189

Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Physics Veterinary Other

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Selection and Identification of Cellulase-Producing Bacteria Isolated from the Litter of Mountain and Swampy Forest . WIZNA; HAFIL ABBAS; YOSE RIZAL; ABDI DHARMA; I PUTU KOMPIANG
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

The isolation and selection of cellulase-producing bacteria was conducted to identify the species of cellulolytic Bacillus. The bacteria were isolated from the litter of swampy forest in Pesisir Selatan and mountain forest in Lembah Anai Tanah Datar. These bacteria were cultivated in selective media to obtain bacteria from the genus Bacillus. Six Bacillus isolates were obtainedfrom swampy forest and three Bacillus isolates from mountain forest. These isolates were cultivated in agar medium with carboxymethylcellulose as the carbon source. Colonies which produced clear zones were assumed to be cellulolytic Bacillus. Based on biochemical and morphological examinations the result indicated that these two isolates were Bacillus coagulans and B. amyloliquefaciens. The cellulase activity of B. coagulans and B. amyloliquefaciens were 0.812 and 1 200 unit ml-1 to C1(b-exoglucanase) respectively, 0.368 and 0.488 unit ml-1 to Cx(b-endoglucanase) respectively.

Characterization of Extracellular Chitinase from Bacterial Isolate 99 and Enterobacter sp. G-1 from Matsue City, Japan MARIA ENDO MAHATA; ABDI DHARMA; IRSAN RYANTO; YOSE RIZAL
Microbiology Indonesia Vol. 2 No. 1 (2008): April 2008
Publisher : Indonesian Society for microbiology

One hundred and twenty isolates of chitosanase producing bacteria were screened from water and soil from localies around Matsue city, Japan. In previous experiments, four isolates (isolates 96, 97, 99, and 100 strain ) were analyzed for their chitosanase characteristics, and one of the isolates (99) was detected as being both a chitosanase and a chitinase producer. Characteristics of the chitinase enzyme were analyzed in this study. Chitinase from bacterial isolate 99 showed higher activities compared to that Enterobacter sp. G-1 (isolated from water in Matsue city, Japan), the activity was 0.039 U/ml and the specific activity was 0.56 U/mg protein, while those from Enterobacter sp. G-1 were 0.029 U/ml and 0.48 U/mg protein respectively. Chitinase from isolate 99 was stable in a pH range between 4-7, while that from Enterobacter sp. G-1 was stable in pH range 3-7. Optimum pH of the chitinase produced by isolate 99 was 5 whereas the chitinase from Enterobacter sp. G-1 it was pH 7. Chitinase from isolate 99 was stable at temperature 20-60°C, while that from Enterobacter sp. G-1 at 20-50°C. Chitinase secreted by isolate 99 showed optimum temperature of 50°C while chitinase from Enterobacter sp. G-1 was optimal at 40°C. Several ions (Fe2+, Ba2+, Co2+) increased the activity of the enzyme from isolate 99 whereas Ca2+ and Co2+ increased activity of the Enterobacter sp. G-1 chitinase..

ISOLASI DAN SKRINING MIKROALGA AIR TAWAR SEBAGAI SUMBER PIGMEN KAROTENOID Arif Juliari Kusnanda; Bayu Afnovandra Perdana; Abdi Dharma; Zulkarnain Chaidir
Jurnal Kimia dan Kemasan Vol. 43 No. 1 April 2021
Publisher : Balai Besar Kimia dan Kemasan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24817/jkk.v43i1.6827

Mikroalga menjadi salah satu sumber senyawa aktif yang memiliki potensi sebagai sumber pangan fungsional sebagai antioksidan seperti karotenoid. Penelitian ini bertujuan untuk mengisolasi mikroalga dari sumber perairan air tawar dan melakukan penapisan terhadap mikroalga sebagi sumber karotenoid. Mikroalga diisolasi dengan menggunakan mikropipet dan dikultivasi dalam media Bold’s Basald Medium (BBM). Penentuan tingkat pertumbuhan dengan spektrofotometer UV-Vis, penentuan berat biomassa kering secara gravimetri, skrining total karotenoid dilakukan menggunakan metode Lichtenthaller dengan spektrofotometer UV-Vis. Berdasarkan hasil penelitian diperoleh empat spesies mikroalga dengan warna hijau yang menandakan semuanya merupakan golongan mikroalga chlorophyta (alga hijau). Skirining total karotenoid dilakukan terhadap empat spesies hasil isolasi dan 2 spesies mikroalga dari Laboratorium Biokimia yaitu Scenedesmus rubescens dan Galdiera sulphuraria. Hasil skrining kandungan total karotenoid tertinggi adalah mikroalga hasil isolasi dari air kolam dengan yang merupakan genus chlorophyta sebesar 36±0,25 μg/g. Hasil ini memberikan informasi isolat mikroalga hasil isolasi dapat dijadikan sebagai mikroalga potensial sebagai sumber dan produksi karotenoid.

IDENTIFIKASI PROFIL FITOKIMIA DAN UJI AKTIFITAS ANTIOKSIDAN EKSTRAK DIETIL ETER DAUN SURIAN (Toona sinensis (A.Juss) M.Roem) DENGAN METODE DPPH. Nurhamidah Nurhamidah; Hazli Nurdin; Yunazar Manjang; Abdi Dharma
ALOTROP Vol 3, No 1 (2019)
Publisher : University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/atp.v3i1.9040

Surian Toona sinensis (A.Juss) M. Roem is a plant that is widely used by the community in the field of medicine such as fever, diarrhea, blood booster, tonic and others. This study aims to determine the secondary metabolite compounds found in leaf extracts of Toona sinensis and test the antioxidant activity using the 2,2-diphenyl-1- (2,4,6-trinitrophenyl) -hydrazinyl (DPPH) method. Identification of secondary metabolites by alkaloids, terpenoids, steroids, flavonoids, phenolics, saponins, coumarin and carotenoids. Toona sinensis leaf was extracted using methanol as a solvent. The resulting extract was saponified with a KOH solution in methanol. The saponification results were extracted again with diethyl ether solvent. The ether layer obtained was washed with distilled water, then dried with Na2SO4 and evaporated to dry, then tested its antioxidant activity. The identification results show Surian leaves contain all secondary metabolites except alkaloids. The antioxidant activity parameters of the Surian extract (Toona sinensis) are indicated by the% inhibitory and IC50 values. Test results showed diethyl ether extract had moderate category of antioxidant activity with IC50 122, 3752 ?g / mL

Penapisan Mikroalga Penghasil Karotenoid serta Studi Pengaruh Stres Nitrogen dan Fosfor terhadap Produksi B-Karoten pada Mikroalga Oocystis sp. Wahida Nia Elfiza; Abdi Dharma; Nasril Nasir
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 1 (2019): Juni 2019
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v14i1.598

Abstrakb-karoten merupakan karotenoid yang bermanfaat sebagai antioksidan. Penelitian ini bertujuan menapis mikroalga yang berpotensi mengandung karotenoid tinggi dan mempelajari pengaruh nitrogen dan fosfor terhadap produktivitas biomassa, kandungan pigmen fotosintesis dan b-karoten, pada mikroalga yang diisolasi dari perairan Danau Atas, Sumatra Barat. Penapisan mikroalga penghasil karotenoid dilakukan dengan memberikan paparan UV-A 326 nm terhadap kultur campuran mikroalga. Hasil penelitian menunjukkan bahwa 5 dari 18 spesies mampu bertahan pada proses penapisan. Pengaruh 9 jenis medium pertumbuhan dengan kriteria: tanpa NaNO3, 3x NaNO3, 5x NaNO3, 10x NaNO3, Bold Basalt Medium (BBM) normal (kontrol), tanpa KH2PO4, 3x KH2PO4, 5x KH2PO4,dan10x KH2PO4 terhadap mikroalga terpilih (Oocystis sp.) diamati. Hasil menunjukkan kandungan b-karoten tertinggi ditemukan pada perlakuan 5x KH2PO4 yaitu sebesar 0,22 % dari berat kering mikroalga, dengan produktivitas biomassa 0,0015 g/mL/hari, serta kandungan klorofil a, klorofil b dan karotenoid total yaitu 7,15 µg/mL, 0,81 µg/mL dan 6,67 µg/mL. Berdasarkan penelitian ini dapat disimpulkan medium pertumbuhan dengan kandungan 5x KH2PO4 merupakan medium yang cocok bagi pertumbuhan Oocystis sp. untuk mendapatkan biomassa dengan kandungan b-karoten dan karotenoid tinggi tanpa harus menurunkan produktivitas biomassanya. Screening of Carotenoid Producing Microalgae and Study of the Effect of Nitrogen and Phosphorus Stress on the Production of b-Carotene in Microalgae Oocystis sp.Abstractb-carotene is a carotenoid that is useful as an antioxidant. Present study aims to screening high microalgae which contain carotenoids from water of Danau Atas lake (West Sumatra Province), and study the effect of nitrogen and phosphorus on biomass productivity, photosynthetic and b-carotene pigment content. Screening of carotenoid-producing microalgae was carried out by exposing UV-A 326 nm to mixed microalgae cultures. The results showed that 5 of the 18 species were able to survive in the screening process. The effect of growth medium, i.e. without NaNO3, 3x NaNO3, 5x NaNO3, 10x NaNO3, normal Bold Basalt Medium (BBM) (control), without KH2PO4, 3x KH2PO4, 5x KH2PO4, and 10x KH2PO4 on of selected microalgae (Oocystis sp.) was carried out. The result showed that the highest content of b-carotene of Oocystis sp. was obtained with 5x KH2PO4 which was 0.22% of the dry weight of biomass. Biomass productivity was 0.0015 g/mL/day and the chlorophyll a, chlorophyll b and total carotenoids contents were 7.15 µg/mL, 0.81 µg/mL and 6.67 µg/mL, respectively. Based on this research, 5x KH2PO4 can be concluded as a suitable medium for Oocystis sp. to obtained high b -carotene and carotenoid of Oocystis sp. without reducing biomass productivity.

PENAPISAN ISOLAT FUNGI MIKORIZA ARBUSKULAR INDIGENUS RIZOSFIR PISANG SEBAGAI INDUSER KETAHANAN TANAMAN PISANG CAVENDISH TERHADAP LAYU FUSARIUM (Fusarium oxysporum f.sp. cubense) Eri Sulyanti; Trimurti Habazar; Eti Farda Husin; Nasril Nasir; Abdi Dharma
JURNAL AGROTROPIKA Vol 16, No 1 (2011): Agrotropika Vol.16 No.1 2011
Publisher : Universitas Lampung

Using biological agents to control fusarium wilt is still not maximal result, based on that need to search a potensial indigeneous biological agents specific location. The experiment conducted to study the role biological agents of arbuscular mychorrhizal fungi indigenous to control fusarium wilt diseases. Twenty four isolates were evaluated for the potensials to reduced fusarium wilt incidence. This research was arranged by Randomized Block Design (RBD) on green house experiment. The aim of this research is to investigate the ability of arbuscular mychorrhizal fungi isolates to reduce fusarium wilt on banana seedling. The results showed that all arbuscular mychorrhizal fungi isolates indigenous from healthy banana rhizosphere reduced fusarium wilt development and increase banana growth.Three isolates (Gl1KeP4, Gl1BuA4, Gl2BuA 6) could control Fusarium wilt until 100% with longer incubation periode and lower disease of banana seedlings as Plant Growth Promoting Fungi. Key words: Arbuscular Mychorrhizal Fungi Indigenous, Fusarium Wilt, Musa sp

Isolasi dan identifikasi spesies mikroalga air tawar sebagai antioksidan dan antihiperglikemik Anggela Marta Tasman; Abdi Dharma; Syafrizayanti Syafrizayanti
Jurnal Litbang Industri Vol 10, No 1 (2020)
Publisher : Institution for Industrial Research and Standardization of Industry - Padang

Mikroalga memiliki kinerja yang hampir sama dengan tumbuhan bersel banyak, akan tetapi tidak memiliki akar, daun, dan batang untuk berfotosintesis. Mikroalga diibaratkan sebagai pabrik kecil dalam ukuran sel mikro yang mengubah karbondioksida menjadi material potensial. Penelitian ini bertujuan untuk mengisolasi mikroalga, mengidentifikasi spesiesnya secara morfologi dan molekuler, selanjutnya menentukan kandungan total fenolik, bioaktivitas antioksidan dan antihiperglikemik dari ekstrak mikroalga tersebut. Uji antioksidan dilakukan dengan metode DPPH dan uji antihiperglikemik dengan metode inhibitor enzim α-amilase. Mikroalga yang berhasil diisolasi termasuk dalam jenis Chlorella vulgaris. Ekstrak metanol dari biomassa kering mikroalga memberikan kandungan fenolik total paling tinggi yaitu sebesar 10,8 mg GAE/g jika dibandingkan ekstrak air (1,8 mg GAE/g) dan heksana (1,1 mg GAE/g). Nilai IC50 ekstrak metanol dalam menangkap radikal bebas DPPH adalah 75,9 µg/mL dan mampu menginhibisi 50% aktivitas enzim α-amilase pada konsentrasi 839,9 µg/mL. Berdasarkan hasil uji bioaktivitas ekstrak metanol mikroalga Chlorella vulgaris yang diisolasi dari perairan Sungai Kincir Kamba Tigo memiliki kemampuan yang rendah sebagai antioksidan dan antihiperglikemik.

Penapisan, isolasi, dan karakterisasi mikroalga yang berpotensi sebagai sumber biodiesel dari perairan Danau Kerinci, Jambi Riska Hernandi; Abdi Dharma; A Armaini
Jurnal Litbang Industri Vol 9, No 1 (2019)
Publisher : Institution for Industrial Research and Standardization of Industry - Padang

Mikroalga menjadi sumber minyak nabati yang berpotensi sebagai bahan baku pembuatan biodiesel dengan kandungan lipid 30-70% dari berat biomassa kering dan komposisi asam lemak yang lengkap. Penelitian ini bertujuan untuk mempelajari keragaman spesies mikroalga dari perairan Danau Kerinci di Jambi, menganalisis kandungan lipid dan asam lemak isolat mikroalga. Mikroalga diisolasi dengan kombinasi teknik goresan, pengenceran berseri, dan mikropipet. Penentuan tingkat pertumbuhan dengan spektrofotometer UV-Vis. Penentuan berat biomassa kering secara gravimetri. Analisis kualitatif lipid dengan uji nile red menggunakan mikroskop flourescence. Analisis kuantitatif lipid dengan ekstraksi menggunakan n-heksana. Analisis kandungan asam lemak dengan alat GC-MS. Terdapat 19 spesies mikroalga yang diidentifikasi dan 2 spesies berhasil ditapis dengan stres salinitas, yaitu MA1 (Scenedesmus rubescens) dan MA2 (Galdieria sulphuraria). MAI dan MA2 memiliki kandungan lipid yang lebih tinggi pada pupuk Growmore dibandingkan pada medium Bold’s Basal. MA1 memiliki kandungan lipid 31,95% pada medium Bold’s Basal dan 32,4% pada pupuk Growmore. MA2 memiliki kandungan lipid 28,72% pada medium Bold’s Basal dan 28,93% pada pupuk Growmore. Mikroalga MA1 dan MA2 dapat dijadikan sumber biodiesel dengan kandungan lipid dan asam lemak jenuh (C16:0, C18:0) yang tinggi.ABSTRACTMicroalgae has been considered recently as a promising biomass feedstock with great potential for biodiesel production with 30-70% lipid content of the dry biomass weight and produces high fatty acid. This research investigated the diversity of microalgae species from waters of Lake Kerinci in Jambi and analysis of the lipid content and fatty acid of microalgae. The isolation was done by agar plate, serial dilution, and micropipette method. The growth rate of the isolated microalgae was determined by UV-Vis spectrophotometer. Dry biomass weight was determined gravimetrically. Nile red staining performed on the isolates to observe the potential of lipid content. Lipids were extracted using n-hexane. Fatty acid analysis by GC-MS. From the results of identification, there were 19 species of microalgae and 2 species were screened with salinity stress. Based on identification of the both isolates, it is known that MA1 isolate is Scenedesmus rubescens and MA2 is Galdieria sulphuraria. MAI and MA2 had higher lipid content in Growmore agrolyzer than Bold’s Basal medium. MA1 had lipid content 31.95% in Bold’s Basal medium and 32.4% in Growmore agrolyzer, MA2 had lipid content 28.72% in Bold’s Basal medium and 28.93 % in Growmore agrolyzer. MA1 and MA2 was a potential as a biodiesel source with high lipid content and saturated fatty acids (C16:0, C18:0).

POTENSI KEBERADAAN SENYAWA AKRILAMIDA DALAM MAKANAN Oktaf Rina; Abdi Dharma; Afrizal Afrizal
Jurnal Analis Farmasi Vol 6, No 2 (2021)
Publisher : Program Studi Analisis Farmasi dan Makanan Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jaf.v6i2.5949

This of the study was to examine the potential presence of acrylamide compounds in foods containing the amino acid asparagine and reducing sugars that are processed at high temperatures. The initial research was carried out to determine the levels of precursors in the raw material and then analyzed the amount of acrylamide using HPLC-RP LC-6A Shimadzu with a C18 column and sterile aqueous mobile phase. The optimization condition of the instrument was achieved with a column temperature of 35oC, a wavelength of 254 nm and a flow rate of 0.8 mL/min. The retention time of acrylamide was observed at 3.8 min. The food sample tested was banana chips. The results showed that banana chips with a moisture content of 7.74% (w/w) ± 0.01 contained acrylamide 115.5 to 565 g/kg. Acrylamide content is influenced by the amount of precursor, temperature and duration of food processing.

Co-Authors - Afrizal Admin Alief Admin Alif Admin Alif Afriyanti Azhar Alif, Admin Anggela Marta Tasman Arif Juliari Kusnanda Armaini - Baharuddin Shaleh Bayu Afnovandra Perdana Candra, Windy Christnatalis Crispin, Andrian Reinaldo Dedi Nofiandi Delima Sitanggang, Delima Edbert, Edbert Edison Munaf Edison Munaf Elida Mardiah Eri Sulyanti Eti Farda Husin Eti Farda Husin Hafil Abbas HAFIL ABBAS Harvianti, Yuniar Hazli Nurdin Heyneker, Daniel Hulu, Victor Trismanjaya Husni Mukhtar I PUTU KOMPIANG I. P. Kompiang Indah Indah Indrawati - Indrawati Indrawati IRSAN RYANTO Jabang Nurdin Jamsari Jamsari Kamble, Pratik Bibhisan Lee Wah Lim MARBUN, ADVENT TORAS Mardi Turnip, Mardi MARIA ENDO MAHATA Marniati Salim Melona Siska Musifa, Eva Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nurhamidah Oktaf Rina Oktoriza, Ghifarizka Periadnadi - Periadnadi Periadnadi PULUNGAN, JURMIDA PURBA, JOICE ANGELINA Rahmadani Wulandari Rahmatika Yani Rahmiana Zein Refilda Refilda Riska Hernandi Saragi, Yosua Morales Sekatresna, Widiyanti Shaleh, Baharuddin Siti Aisyah Siti Hajjir, Siti Sulyanti, Eri Sumaryati Syukur Suryani Suryani Syafriza Yanti Syafrizayanti, Syafrizayanti Syafrizayanti, Syafrizayanti Syafrizayanti, Syafrizayanti Syukri Arief Talib, Ramanisa Muliani Tania, Alinda Tarigan, Julio Putra Toyohide Takeuchi TRIMURTI HABAZAR Turnip, Josua Presen Valentino, Bue Vanness, Jeff Veron, Veron Wahida Nia Elfiza Warni, Mega Waruwu, Jefrin Widiyanti Sekatresna Wijaya, Eko Bambang Wijaya, Jeremy Wijaya, Vincent Wizna (Wizna) Wulandari, Rahmadani Yetria Rilda Yose Rizal Yoserizal Yoserizal Yunazar Manjang Yunazar Manjang Yunazar Manjang Zulkarnain Chaidir

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