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Pengaruh Pemberian Ekstrak Daging Buah Kurma Ajwa (phoenix dactylifera) Terhadap Hitung Jenis Leukosit Embrio dan Induk Mencit (mus musculus) Bunting Eva Agustina; Nova Lusiana; Risa Purnamasari
Biotropic : The Journal of Tropical Biology Vol. 3 No. 2 (2019): Biotropic, Volume 3 Nomor 2, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (763.157 KB) | DOI: 10.29080/biotropic.2019.3.2.135-145

Abstract

Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak daging buah Kurma Ajwa (Phoenix dactylifera) terhadap hitung jenis leukosit embrio dan induk mencit (Mus musculus) bunting. Setiap perlakuan dibagi dalam empat kelompok perlakuan ekstrak. Kelompok I (kelompok kontrol), kelompok II (3 kurma setara 3,12 mg/kg BB mencit), kelompok III (5 kurma setara 5, 2 mg/kg BB mencit), dan kelompok IV (7 kurma setara 7, 28 mg/kg BB mencit). Ekstrak diberikan pada masa kebuntingan 14 sampai 18 hari melalui peroral. Pada hari ke 19 kebuntingan dilakukan pembedahan untuk diambil darahnya pada bagian jantung mencit. Hitung jenis leukosit melalui teknik apusan darah dengan mengamati 100 sel leukosit perbesaran 400 kali pada mikroskop. Hasilnya ekstrak daging buah Kurma Ajwa (P. dactylifera) berpengaruh signifikan terhadap hitung limfosit dan basofil, tetapi tidak berpengaruh terhadap hitung monosit, eosinofil dan neutrofil pada embrio mencit. Sedangkan pada induk mencit berpengaruh terhadap hitung eosinofil dan monosit dan tidak berpengaruh terhadap limfosit, basofil dan neutrofil. Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak daging buah Kurma Ajwa (Phoenix dactylifera) terhadap hitung jenis leukosit embrio dan induk mencit (Mus musculus) bunting. Setiap perlakuan dibagi dalam empat kelompok perlakuan ekstrak. Kelompok I (kelompok kontrol), kelompok II (3 kurma setara 3,12 mg/kg BB mencit), kelompok III (5 kurma setara 5, 2 mg/kg BB mencit), dan kelompok IV (7 kurma setara 7, 28 mg/kg BB mencit). Ekstrak diberikan pada masa kebuntingan 14 sampai 18 hari melalui peroral. Pada hari ke 19 kebuntingan dilakukan pembedahan untuk diambil darahnya pada bagian jantung mencit. Hitung jenis leukosit melalui teknik apusan darah dengan mengamati 100 sel leukosit perbesaran 400 kali pada mikroskop. Hasilnya ekstrak daging buah Kurma Ajwa (P. dactylifera) berpengaruh signifikan terhadap hitung limfosit dan basofil, tetapi tidak berpengaruh terhadap hitung monosit, eosinofil dan neutrofil pada embrio mencit. Sedangkan pada induk mencit berpengaruh terhadap hitung eosinofil dan monosit dan tidak berpengaruh terhadap limfosit, basofil dan neutrofil.
Basal Body Temperature Difference Before and During The Endometrial Wall Decay on The Menstrual Cycle Ika Mustika; Nova Lusiana; Estri Kusumawati; Risa Purnamasari
Journal of Health Science and Prevention Vol. 2 No. 1 (2018): JHSP Vol 2 No 1 - 2018
Publisher : State Islamic University of Sunan Ampel

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (559.206 KB) | DOI: 10.29080/jhsp.v2i1.112

Abstract

The menstrual cycle is a complex process involving hormones and reproductive organs. Hormonal changes that occur in the menstrual cycle result in changes in body basal temperature in women. Basal body temperatures can identify phase changes in one menstrual cycle. This study is to determine whether there is influence of decay of the endometrium wall against the basal body temperature in adolescent girls. This research is observational analytic with cross sectional time approach. This study was conducted on 60 samples with the age of 19-22 years. Data used in this research are primary data that is basal body temperature measurement and questioner. The analysis in this study is paired sample t test test using SPSS 16. The results obtained significance value of 0.003 <0.05, so it can be concluded that there is influence of decay of the endometrium wall in the menstrual cycle against changes in basal body temperature. The endometrial wall decay on the menstrual cycle causes basal body temperature decreased compare with before the uterine lining decay or menstruation.
Analisis Kuantitatif Kandungan Antioksidan dan Aktivitas Sitotoksik dari Ekstrak Anastatica hierochuntica L Nova Lusiana; Risa Purnamasari; Eva Agustina; Nurul Ilmi Faidah; Azlinda Mitha Agustin; Muhammad Falikhul M
Biotropic : The Journal of Tropical Biology Vol. 6 No. 2 (2022): Biotropic, Volume 6 Nomor 2, 2022
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Antioxidants are compounds that can counteract free radicals in the body due to the presence of active compounds such as flavonoids and phenolics. These active compounds can be cytotoxic, ie, compounds capable of inhibiting and stopping the growth of cancer cells by using toxicity test by BSLT (Brine Shrimp Lethality Test) method. This study aims to determine the antioxidant activity by DPPH (Dipheny Pycryl Hydrazil) method and toxicity test by BSLT method of Anastatica hierochuntica L extract. A. hierochuntica L extraction were done by maceration method with methanol solvent. The content of A. hierochuntica L extract compounds was done by phytochemical test to know flavonoid compounds, triterpenoid and sterol. Meanwhile, alkaloid and saponin and liquid chromatography to see flavonoid compounds of quersetin. Furthermore, the antioxidant activity test was carry out by the DPPH method and by BSLT method to observed the mortality of shrimp larvae. A. hierochuntica extract has antioxidant activity with IC50 value 77,23 μg / ml and toxicity value of LC50 44,97. A.hierochuntica L extracts potentially have antioxidant and cytotoxic properties.
Efek Kombinasi Linear Alkylbenzene Sulfonates (LAS) dan Tembaga (Cu) pada Kapasitas Fitoremediasi Tanaman Daun Tombak (Sagittaria lancifolia) Eva Agustina; Salsa Nabila; Risa Purnamasari; Rony Irawanto
BEST Journal (Biology Education, Sains and Technology) Vol 6, No 1 (2023): Juni 2023
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v6i1.6912

Abstract

Salah satu masalah lingkungan yang serius saat ini adalah pencemaran air yang dapat membahayakan kesehatan manusia dan organisme lainnya. Salah satu penyebab pencemaran air adalah adanya detergen dan logam berat yang terdapat di lingkungan. Karena dampaknya yang merugikan, perlu dicari solusi untuk menangani masalah ini. Salah satu solusinya adalah menggunakan teknik fitoremediasi dengan memanfaatkan tanaman daun tombak (Sagittaria lancifolia) yang terbukti mampu menyerap berbagai polutan dari lingkungan. Oleh karena itu, penelitian ini bertujuan untuk mengevaluasi efektivitas fitoremediasi tanaman daun tombak (Sagittaria lancifolia) dalam menangani kontaminasi detergen LAS (Linear Alkylbenzene Sulfonates) dan logam berat tembaga (Cu) pada air. Penelitian ini menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL) dengan empat perlakuan berbeda konsentrasi LAS + Cu, yaitu 0 mg/L, 10 mg/L + 3 mg/L, 30 mg/L + 3 mg/L, dan 50 mg/L + 3 mg/L. Hasil penelitian menunjukkan daya penyisihan LAS tertinggi sebesar 96% pada variasi konsentrasi LAS 10 mg/L + Cu 3 mg/L, dan daya penyisihan Cu tertinggi sebesar 72% pada variasi konsentrasi  LAS 50 mg/L + Cu 3 mg/L. lebih lanjut, nilai efektifitas penyerapan logam Cu tertinggi sebesar 59% pada variasi konsentrasi  LAS 50 mg/L + Cu 3 mg/L. Hasil penelitian juga menunjukkan semakin tinggi LAS yang diberikan maka semakin tinggi pula penyerapan logam berat Cu oleh tanaman. Berdasarkan hasil tersebut, terbukti bahwa tanaman daun daun tombak (Sagittaria lancifolia) efektif untuk menyerap LAS dan logam berat Cu di lingkungan.
Efektivitas Tanaman Daun Tombak (Sagittaria lancifolia) dalam Fitoremediasi Linear Alkylbenzene Sulfonates (LAS) pada Media Air Salsa Nabila; Eva Agustina; Risa Purnamasari; Rony Irawanto
BIOMA: Jurnal Biologi dan Pembelajarannya Vol 5 No 1 (2023): BIOMA: Jurnal Biologi dan Pembelajarannya
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/bioma.v5i1.2489

Abstract

Pencemaran air merupakan masalah lingkungan yang serius yang mengancam kesehatan manusia dan lingkungan di seluruh dunia. Salah satu pencemar yang sering dijumpai dilingkungan adalah detergen. Adanya pencemaran air dapat mengancam kesehatan berbagai organisme termasuk manusia. Untuk itu, diperlukan solusi untuk menanggulangi permasalahan tersebut. Salah satu teknik yang dapat digunakan adalah dengan fitoremediasi menggunakan tanaman daun tombak (Sagittaria lancifolia). Tanaman ini terbukti mampu meyerap berbagai polutan yang ada di lingkungan. Oleh karena itu, penelitian ini bertujuan untuk mengetahui efektifitas tanaman daun tombak (Sagittaria lancifolia) dalam fitoremediasi detergen LAS (Linear Alkylbenzene Sulfonates) pada media air. Metode yang digunakan adalah eksperimental dengan Rancangan Acak Lengkap (RAL) dengan empat perlakuan berbeda konsentrasi LAS, yaitu 0 mg/L, 10 mg/L, 30 mg/L, dan 50 mg/L. Hasil penelitian menunjukkan tanaman daun tombak (Sagittaria lancifolia) efektif dalam menyerap kandungan LAS (Linear Alkylbenzene Sulfonates) dalam air dengan daya penyisihan polutan paling tinggi sebesar 95% pada air yang tercemar LAS 10 mg/L. Nilai pH dan TDS mengalami peningkatan tiap minggu pada tiap konsentrasi dan kadar COD meningkat tiap konsentrasi seiring dengan peningkatan kandungan LAS. Namun, hasil pengukuran ketiga parameter masih sesuai dan tidak melebihi ambang batas baku mutu yang telah ditetapkan sehingga dapat dikatakan tanaman sagitaria lancifolia dapat menstabilakan kualitas air yang tercemar LAS.
The Impact of Immunity on Developing Literacy Culture at Primary School: Investigating Students’ Extroverted and Introverted Personalities Evi Fatimatur Rusydiyah; Misbakhul Munir; Hernik Farisia; Hanik Faizah; Risa Purnamasari
Journal of Innovation in Educational and Cultural Research Vol 4, No 2 (2023)
Publisher : Yayasan Keluarga Guru Mandiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46843/jiecr.v4i2.542

Abstract

The development of literary culture in schools needs a literate ecosystem and full support from literacy activists in schools. This study aims to describe and explore the profile of literacy activists in madrasahs in terms of their level of immunity and personality traits. This study investigates the effect of student immunity as literacy activists in madrasahs with extroverted and introverted personality types on the ability to develop a literary culture. The parameters used to investigate the immunity levels in extroverted and introverted students were by examining the number of leukocytes, types of leukocytes, and the results of the IL-6 examination using the ELISA method. The results show that extroverted students have higher immunity than introverted students. The average leukocyte level of extroverted students is 24,350 cells/mm3 and introverted students is 50 cells/mm3. Meanwhile, the results of the IL-6 examination show the highest is 163,863 and the lowest is 43,984. Extroverted students can design various literacy programs that are challenging for students and are better able to build networks in developing literacy programs compared to introverted students. So, it can be concluded that extroverted students have a higher level of immunity, and also have the ability to explore and develop literacy programs better than introverted students.
Determining the Effect of Orange Peel Extract in Water on Total Cholesterol Fluctuations in HFD-Induced Mice Ria Qadariah Arief; Linda Prasetyaning W; Risa Purnamasari; Sarita Oktorina; Sri Hidayati L
Journal of Health Science and Prevention Vol. 7 No. 2 (2023): JHSP vol 7 No 2 - 2023
Publisher : State Islamic University of Sunan Ampel

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/jhsp.v7i2.1079

Abstract

Hypercholesterolemia, characterized by elevated levels of total cholesterol, is a well-established risk factor for cardiovascular diseases, necessitating the exploration of natural interventions to mitigate dyslipidemia and its associated health risks. This study aimed to investigate the potential influence of orange peel extract in water on total cholesterol fluctuations in HFD-induced mice. This study used a true experimental design to investigate the effects of dietary interventions on total cholesterol levels in a female mice model. A total of 18 adult mice were utilized for this research, and they were divided into three distinct groups. The allocation of mice into these groups was as follows: Control Group (n=6): The first group served as the control and received only Aquades (sterile water). High-Fat Diet (HFD) Group (n=6) as named HFD only: The second group consisted of mice that were exclusively fed a high-fat diet (HFD) for a total of 17 days. Orange Peel Extract Group (n=6): The third group, also exposed to the HFD for an initial 10-day period, was subsequently administered orange peel extract for seven days. The total cholesterol levels of the mice that were given orange peel extract decreased from 109 ± 7.43 mg/dL to 96.5 ± 10.07 mg/dL after the intervention. This decrease was statistically significant (p < 0.05). the risk of high total cholesterol levels in mice that were given orange peel extract was 33.3%, compared to 83.3% in mice that were given aquades and 50% in mice that were still on a high-fat diet (HFD). , the results of this study suggest that orange peel extract may offer a natural and accessible approach to address dyslipidemia and reduce the risk of cardiovascular diseases. While further research is needed to confirm these findings and explore their applicability to human health.
The Influence of Discovery Learning on Serotonin Hormones and Student Learning Achievements Husniyatus Salamah Zainiyati; Evi Fatimatur Rusydiyah; Hanik Faizah; Hernik Farisia; Risa Purnamasari; Qurrotul Ayun
Journal of Innovation in Educational and Cultural Research Vol 4, No 4 (2023)
Publisher : Yayasan Keluarga Guru Mandiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46843/jiecr.v4i4.1186

Abstract

Discovery learning is a model designed to tackle the issue of low learning achievement. However, typically, research on discovery learning mainly focuses on outcomes to enhance specific abilities partially. Yet, discovery learning is also associated with physiological aspects. This study aims to determine the discovery of learning influence on learning achievement and serotonin. This is an experimental study. The independent variable (x) is discovery learning, whereas the dependent variables (y) are student achievement and serotonin. The statistical test used the paired sample t-test. Other data related to students' learning skills in the classroom using discovery learning were observed and analyzed with the descriptive qualitative method. There were significant differences in learning achievement and serotonin levels before and after the implementation of discovery learning.The average scores before and after the implementation of discovery learning were 46.75 and 78.55, respectively. The average hormone levels before and after the implementation of discovery learning were 719.25 and 1420.59 ng/mL, respectively. The implementation of discovery learning increases serotonin levels, thus optimizing student learning achievement. Following this research finding, research on how to increase serotonin levels through other learning methods needs to be conducted to explore many best practices that affect students' achievement.
Assessing the Tolerance of Sagittaria Lancifolia (Spear Plant) to Linear Alkylbenzene Sulfonates (LAS) Exposure Salsa Nabila; Risa Purnamasari; Eva Agustina; Rony Irawanto
Indonesian Journal of Biology Education Vol. 6 No. 1 (2023): INDONESIAN JOURNAL OF BIOLOGY EDUCATION
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

One of pollutant that commonly found in aquatic environment is detergent. Detergent pollution can cause skin irritation and other health problems in humans and animals that use contaminated water sources. To address this issue, it is critical to implement effective policies and regulations to control detergent pollution and promote sustainable wastewater management practices in Indonesia. One of the approaches to overcome detergent pollution is to use plants as pollutant absorbing agents, or commonly called phytoremediation techniques. Therefore, this study aims to determine the growth response of spear plant (Sagittaria lancifolia) in response to the stress of Linear Alkylbenzene Sulfonate (LAS) detergent. This research is an experimental research using a completely randomized design (CRD). There were four treatments tested with three replicates for each treatment. Treatment variations were based on the concentrations used in this study, namely 0 mg/L, 10 mg/L, 30 mg/L, and 50 mg/L. The research was conducted for four months, starting from July to October 2022, and was conducted in the greenhouse of Purwodadi Botanical Garden – BRIN. The result showed that although it experienced some morphological changes such as leaf drying, discoloration, and structural changes, Sagittaria lancifolia were able to survive and conduct metabolic activities properly during 28 days of Linear Alkylbenzene Sulfonates (LAS) pollutant stress.  Thus, Sagittaria lancifolia are able to be used as one of the strategies in restoring the aquatic environment polluted by domestic waste, including LAS detergent.
Pengaruh Paparan Linear Alkylbenzene Sulfonates (LAS) dan Logam Berat Tembaga (Cu) terhadap Pertumbuhan Sagittaria lancifolia Nabila, Salsa; Purnamasari, Risa; Agustina, Eva; Irawanto, Rony
Jurnal Jeumpa Vol 10 No 1 (2023): Jurnal Jeumpa
Publisher : Department of Biology Education, Faculty of Teacher Training and Education, Samudra University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jj.v10i1.7537

Abstract

Berbagai jenis limbah dapat menyebabkan pencemaran perairan, termasuk limbah domestik yang mengandung surfaktan LAS dan limbah industri yang mengandung logam berat seperti tembaga (Cu). Kadar pencemar yang tinggi dapat mempengaruhi metabolisme dan menyebabkan kematian pada organisme. Salah satu solusi untuk mengatasi pencemaran ini adalah dengan fitoremediasi, di mana tanaman Sagittaria lancifolia digunakan untuk menyerap pencemar LAS dan logam Cu. Namun, respon pertumbuhan tanaman ini terhadap pencemaran LAS dan logam Cu belum diketahui dengan detail. Untuk itu, penelitian ini bertujuan mengetahui adanya paparan LAS dan logam Cu terhadap respon pertumbuhan tanaman Sagittaria lancifolia. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 ulangan, di mana setiap perlakuan memiliki konsentrasi pencemar yang berbeda, yaitu 0 ppm (kontrol), LAS 10 ppm + Cu 3 ppm, LAS 30 ppm + Cu 3 ppm, dan LAS 50 ppm + Cu 3 ppm. Hasil penelitian menunjukkan bahwa paparan LAS dan logam Cu pada konsentrasi yang berbeda tidak memiliki efek signifikan terhadap pertumbuhan dan morfologi tanaman Sagittaria lancifolia. Oleh karena itu, tanaman tersebut memiliki potensi sebagai opsi untuk fitoremediasi perairan yang tercemar oleh LAS dan logam Cu.