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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biology, Medicine, & Natural Product Chemistry International Journal of Applied Biology Health Notions JRST (Jurnal Riset Sains dan Teknologi) Journal of Islamic Medicine Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Journal of Health Science and Prevention Best Journal (Biology Education, Sains and Technology) Journal of Innovation in Educational and Cultural Research Indian Journal of Forensic Medicine & Toxicology Jurnal Jeumpa STRATEGY : Jurnal Inovasi Strategi dan Model Pembelajaran BIOMA : Jurnal Biologi dan Pembelajarannya Jurnal Sosial dan Sains Zona Education Indonesia Indonesian Journal of Biology Education BIOTROPIC
Risa Purnamasari
Universitas Islam Negeri Sunan Ampel Surabaya
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Pengaruh Paparan Linear Alkylbenzene Sulfonates (LAS) dan Logam Berat Tembaga (Cu) terhadap Pertumbuhan Sagittaria lancifolia Nabila, Salsa; Purnamasari, Risa; Agustina, Eva; Irawanto, Rony
Jurnal Jeumpa Vol 10 No 1 (2023): Jurnal Jeumpa
Publisher : Department of Biology Education, Faculty of Teacher Training and Education, Samudra University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jj.v10i1.7537

Abstract

Berbagai jenis limbah dapat menyebabkan pencemaran perairan, termasuk limbah domestik yang mengandung surfaktan LAS dan limbah industri yang mengandung logam berat seperti tembaga (Cu). Kadar pencemar yang tinggi dapat mempengaruhi metabolisme dan menyebabkan kematian pada organisme. Salah satu solusi untuk mengatasi pencemaran ini adalah dengan fitoremediasi, di mana tanaman Sagittaria lancifolia digunakan untuk menyerap pencemar LAS dan logam Cu. Namun, respon pertumbuhan tanaman ini terhadap pencemaran LAS dan logam Cu belum diketahui dengan detail. Untuk itu, penelitian ini bertujuan mengetahui adanya paparan LAS dan logam Cu terhadap respon pertumbuhan tanaman Sagittaria lancifolia. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 ulangan, di mana setiap perlakuan memiliki konsentrasi pencemar yang berbeda, yaitu 0 ppm (kontrol), LAS 10 ppm + Cu 3 ppm, LAS 30 ppm + Cu 3 ppm, dan LAS 50 ppm + Cu 3 ppm. Hasil penelitian menunjukkan bahwa paparan LAS dan logam Cu pada konsentrasi yang berbeda tidak memiliki efek signifikan terhadap pertumbuhan dan morfologi tanaman Sagittaria lancifolia. Oleh karena itu, tanaman tersebut memiliki potensi sebagai opsi untuk fitoremediasi perairan yang tercemar oleh LAS dan logam Cu.
Subchronic toxicity test of black garlic (Allium sativum) ethanol extract in liver histology of female mice (Mus musculus) Huwaidah, Jihan Farhah; Agustina, Eva; Purnamasari, Risa
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 7, No 1 (2024): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v7i1.2922

Abstract

Black garlic is an innovative product from garlic which contains S-allyl cysteine (SAC), flavonoids, and polyphenols during the aging process. The use of black garlic as a medicine has not been studied regarding its toxicity to the liver, so research is needed regarding the safety of its use through toxicity tests. The purpose of this study was to determine the effect of giving various doses of black garlic ethanol extract on liver histology and to determine the safe dosage of black garlic ethanol extract for consumption. The method used was the treatment of black garlic ethanol extract in the treatment group at doses of 5, 50, 100, 300, 600, 1000, and 2000 mg/kg BW orally in mice to determine the effect on the histology of the mice's liver. The results showed that the greater the treatment dose of black garlic ethanol extract, the greater the percentage of damage to hepatocyte cells in the form of necrosis in the histology of the mice liver. The highest percentage of damaged cells experiencing necrosis was 49% at a dose of 2000 mg/kg BW. The use of doses that are safe for consumption has not been found in this study due to cell damage found > 30% in the category of moderate damage. So further testing is needed using a lower dose or by administering the extract over a shorter period of time to determine the safe dose for consumption.
Identifikasi Senyawa Metabolit Sekunder pada Ekstrak Etanol Daun Binahong Aliyyah Nafiisah; Risa Purnamasari; Siti Mudalianah
Jurnal sosial dan sains Vol. 4 No. 11 (2024): Jurnal Sosial dan Sains
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/jurnalsosains.v4i11.27922

Abstract

Latar Belakang: Tanaman Binahong (Anredera Cordifolia) dalam famili Basellaceae merupalan tanaman yang memiliki bahan fitokarma. Tanaman daun binahong memiliki banyak manfaat seperti mengobati kerusakan ginjal, pemulihan luka, melancarkan tekanan darah dan lain sebagainya. Tujuan: Tujuan dari penelitian ini untuk mengidentifikasi senyawa metabolit sekunder pada Ekstrak Etanol 70 % daun binahong yang telah disimpan selama 12 bulan. Metode: Penelitian bersifat deskriptif kualitatif yang dilakukan dengan menggunakan beberapa reagen, disesuaikan dengan golongan fitokimia (alkaloid, fenolik, flavonoid, saponin, tanin, steroid & triterpenoid). Ekstrak Daun Binahong dilakukan proses penyarian dengan metode maserasi. Ekstrak Daun binahong selanjutnya dilakukan uji fitokimia untuk mengetahui kandungan senyawa metabolit sekunder. Hasil: Hasil dari penelitian ini menunjukkan bahwa pada daun binahong yang telah disimpan selama 12 bulan masih terkandungan senyawa metabolit sekunder yaitu flavonoid, tannin, alkaloid dan saponin. Hal ini dapat menjelaskan ekstrak etanol dapat mempertahankan senyawa metabolit sekunder yang terkandung dalam ekstrak sampai 12 bulan penyimpanan Kesimpulan: Metode maserasi dengan pelarut etanol 70% terbukti efektif dalam mempertahankan senyawa metabolit sekunder selama penyimpanan jangka panjang. Hasil ini dapat menjadi dasar untuk pengembangan metode penyimpanan optimal pada ekstrak tanaman obat, mendukung praktik pengobatan tradisional dan formulasi farmasi berbasis bahan alami.
Penerapan Aplikasi Geogebra Terhadap Motivasi dan Pemahaman Konsep Matematika Siswa Materi Bangun Ruang Sisi Datar Purnamasari, Risa; Supardi
Zona Education Indonesia Vol. 3 No. 3 (2025): June 2025
Publisher : Yayasan Mentari Madani

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to analyze the effect of using the GeoGebra application on students’ motivation and conceptual understanding of mathematics in the topic of flat-faced solid shapes. The research employed an experimental method with a pretest-posttest control group design. The sample consisted of two classes divided into an experimental group, which used GeoGebra in learning, and a control group, which applied conventional teaching methods. Data were collected through conceptual understanding tests, motivation questionnaires before and after treatment, and classroom activity observations. The results showed that the experimental group experienced a significant increase in mathematics conceptual understanding with an average n-gain of 0.65 (medium to high category), compared to the control group, which only achieved an n-gain of 0.41 (low to medium category). Additionally, the motivation of students in the experimental group increased significantly, with 78% of students reaching a high motivation category, while the control group had only 42%. Observations revealed that GeoGebra encouraged students to actively discuss, explore, and interact with the learning material. Based on these findings, it can be concluded that the application of GeoGebra is effective in improving both motivation and conceptual understanding in mathematics learning on the topic of flat-faced solid shapes. This study recommends educators to integrate visual technology-based learning tools to enhance the quality of mathematics teaching and learning processes
Formulation and Characterization Tests of SCOBY Kombucha Gel Agustina, Eva; Nafisah, Raihana Frika; Fiddaroini, Achmad Farchan; Tania, Clarista Eka; Purnamasari, Risa; Andiarna, Funsu; Hidayati, Irul
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.143-151

Abstract

The wound is the damage to body tissues caused by various factors such as accidents, sharp object scratches, etc. Proper care is necessary to accelerate the wound healing process. Topical medications, such as gel preparations, are commonly used for wounds. Kombucha SCOBY is believed to contain bioactive compounds that can accelerate the wound healing process, making it a potential active ingredient in gel preparations. This research aims to determine the characteristics of kombucha SCOBY gel with various concentration variations. SCOBY is obtained from kombucha fermentation using green tea, sugar, and kombucha culture. Preparing kombucha SCOBY gel involves using carbomer 940, methyl paraben, triethanolamine, glycerin, and SCOBY kombucha. The concentration variations of kombucha SCOBY gel include 0%, 2%, 4%, 8%, 10%, and 12%. Each formula undergoes physical characteristic tests including an organoleptic test, hedonic test, homogeneity test, pH test, spreadability test, and adhesiveness test. Data obtained after physical characteristic tests of the gel preparations are analyzed descriptively. Based on the conducted research, it was found that kombucha SCOBY gel preparations with concentration variations of 0%, 2%, 4%, 8%, 10%, and 12% exhibited different characteristics. The higher the gel concentration, the more acidic the gel's aroma, the more liquid the texture, and the more intense the color. SCOBY added to the gel preparation can increase the pH value, adhesiveness, and spreadability of the gel.
Effect of Variation in Concentration of SCOBY Kombucha Gel Citrus Peel Extract (Citrus nobilis) on Wound Incision (Vulnus Scissum) in Mice (Mus musculus) Shofiyya, Ainina Neihayatus; Eva Agustina; Funsu Andiarna; Irul Hidayati; Risa Purnamasari
International Journal of Applied Biology Vol. 9 No. 1 (2025): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

A laceration wound is damage to the skin tissue characterized by a neat outline of the wound edge, due to slices by sharp instruments or incisions during surgery. This study aims to evaluate the effect of variations in the concentration of SCOBY Kombucha gel preparations of orange peel extract (Citrus nobilis) on wound healing (Vulnus Scissum) in mice (Mus musculus). SCOBY Kombucha gel was made with six concentration variations (0%, 2%, 4%, 8%, 10%, and 12%) and tested for its effectiveness in accelerating wound healing. The research method involved making incision wounds on male mice, followed by applying the gel according to the specified concentrations. The results showed that SCOBY Kombucha gel with a concentration of 12% was most effective in accelerating wound healing, approaching the effectiveness of the positive control (bioplacenton). Analysis showed that the increase in gel concentration was directly proportional to the effectiveness of wound healing, where higher concentration gels showed significantly faster wound closure.
Efek Kombinasi Linear Alkylbenzene Sulfonates (LAS) dan Tembaga (Cu) pada Kapasitas Fitoremediasi Tanaman Daun Tombak (Sagittaria lancifolia) Agustina, Eva; Nabila, Salsa; Purnamasari, Risa; Irawanto, Rony
BEST Journal (Biology Education, Sains and Technology) Vol 6, No 1 (2023): Juni 2023
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v6i1.6912

Abstract

Salah satu masalah lingkungan yang serius saat ini adalah pencemaran air yang dapat membahayakan kesehatan manusia dan organisme lainnya. Salah satu penyebab pencemaran air adalah adanya detergen dan logam berat yang terdapat di lingkungan. Karena dampaknya yang merugikan, perlu dicari solusi untuk menangani masalah ini. Salah satu solusinya adalah menggunakan teknik fitoremediasi dengan memanfaatkan tanaman daun tombak (Sagittaria lancifolia) yang terbukti mampu menyerap berbagai polutan dari lingkungan. Oleh karena itu, penelitian ini bertujuan untuk mengevaluasi efektivitas fitoremediasi tanaman daun tombak (Sagittaria lancifolia) dalam menangani kontaminasi detergen LAS (Linear Alkylbenzene Sulfonates) dan logam berat tembaga (Cu) pada air. Penelitian ini menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL) dengan empat perlakuan berbeda konsentrasi LAS + Cu, yaitu 0 mg/L, 10 mg/L + 3 mg/L, 30 mg/L + 3 mg/L, dan 50 mg/L + 3 mg/L. Hasil penelitian menunjukkan daya penyisihan LAS tertinggi sebesar 96% pada variasi konsentrasi LAS 10 mg/L + Cu 3 mg/L, dan daya penyisihan Cu tertinggi sebesar 72% pada variasi konsentrasi  LAS 50 mg/L + Cu 3 mg/L. lebih lanjut, nilai efektifitas penyerapan logam Cu tertinggi sebesar 59% pada variasi konsentrasi  LAS 50 mg/L + Cu 3 mg/L. Hasil penelitian juga menunjukkan semakin tinggi LAS yang diberikan maka semakin tinggi pula penyerapan logam berat Cu oleh tanaman. Berdasarkan hasil tersebut, terbukti bahwa tanaman daun daun tombak (Sagittaria lancifolia) efektif untuk menyerap LAS dan logam berat Cu di lingkungan.
Biosynthesis of Gold Nanoparticles using Fig Leaf (Ficus Carica) Isolate as a Reducing Agent Lusiana, Nova; Ma'arif, Muhammad; Agustina, Eva; Purnamasari, Risa
Journal of Health Science and Prevention Vol. 8 No. 2 (2024): JHSP Vol 8 No 2 – 2024
Publisher : State Islamic University of Sunan Ampel

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/jhsp.v8i2.290

Abstract

Proper and swift treatment is crucial for cancer patients. The fig plant contains antioxidants that show inhibition of cancer cell proliferation. By converting its particle size into nano, the active compounds are expected to more easily reach the target cells. The aim of this study is to determine the effectiveness of gold nanoparticles with a reducing agent from fig leaf isolate (Ficus carica). The research method used is laboratory experimentation to produce gold nanoparticles with fig leaf extract as the reducing agent, and the antioxidant activity was tested by observing the IC50 value. The results of the study showed that gold nanoparticles from fig leaf extract were successfully created, as evidenced by UV-Vis spectrophotometer testing at a wavelength of 528 nm. The antioxidant test demonstrated that gold nanoparticles from fig leaf extract have greater potential as an antioxidant agent compared to fig leaf extract alone.
Identifikasi Senyawa Metabolit Sekunder pada Ekstrak Etanol Daun Binahong Nafiisah, Aliyyah; Purnamasari, Risa; Mudalianah, Siti
Jurnal sosial dan sains Vol. 4 No. 11 (2024): Jurnal Sosial dan Sains
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/jurnalsosains.v4i11.27922

Abstract

Latar Belakang: Tanaman Binahong (Anredera Cordifolia) dalam famili Basellaceae merupalan tanaman yang memiliki bahan fitokarma. Tanaman daun binahong memiliki banyak manfaat seperti mengobati kerusakan ginjal, pemulihan luka, melancarkan tekanan darah dan lain sebagainya. Tujuan: Tujuan dari penelitian ini untuk mengidentifikasi senyawa metabolit sekunder pada Ekstrak Etanol 70 % daun binahong yang telah disimpan selama 12 bulan. Metode: Penelitian bersifat deskriptif kualitatif yang dilakukan dengan menggunakan beberapa reagen, disesuaikan dengan golongan fitokimia (alkaloid, fenolik, flavonoid, saponin, tanin, steroid & triterpenoid). Ekstrak Daun Binahong dilakukan proses penyarian dengan metode maserasi. Ekstrak Daun binahong selanjutnya dilakukan uji fitokimia untuk mengetahui kandungan senyawa metabolit sekunder. Hasil: Hasil dari penelitian ini menunjukkan bahwa pada daun binahong yang telah disimpan selama 12 bulan masih terkandungan senyawa metabolit sekunder yaitu flavonoid, tannin, alkaloid dan saponin. Hal ini dapat menjelaskan ekstrak etanol dapat mempertahankan senyawa metabolit sekunder yang terkandung dalam ekstrak sampai 12 bulan penyimpanan Kesimpulan: Metode maserasi dengan pelarut etanol 70% terbukti efektif dalam mempertahankan senyawa metabolit sekunder selama penyimpanan jangka panjang. Hasil ini dapat menjadi dasar untuk pengembangan metode penyimpanan optimal pada ekstrak tanaman obat, mendukung praktik pengobatan tradisional dan formulasi farmasi berbasis bahan alami.
Effect of Different Concentration of SCOBY Kombucha as an Anti-acne Against Staphylococcus aureus in The Ear of Mice (Mus musculus) Ferdyanti, Anggun Putri; Agustina, Eva; Faizah, Hanik; Purnamasari, Risa
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1143-1150

Abstract

SCOBY (Symbiotic Culture of Bacteria and Yeast) results from symbiosis between yeast and bacteria during fermentation. SCOBY kombucha gel combined with orange peel extract has antibacterial compounds that can inhibit the growth of S. aureus bacteria. The method in this study was to make variations in the concentration of SCOBY in the gel and inject S. aureus bacteria into the ears of mice. After 2x24 hours, the diameter of the inflammation caused was observed and SCOBY kombucha gel was applied (0%, 2%, 4%, 8%, 10%, and 12%), positive control (Mediclin 1%), and negative control (without gel application). The results showed that the higher the concentration of SCOBY, the faster the healing activity of the gel against S. aureus. SCOBY kombucha gel has the fastest healing activity in inhibiting acne pathogen S. aureus 12% SCOBY gel. Inflammation in the ears of mice treated with 12% SCOBY gel had healed by the 90th hour, while in other groups of mice it had only healed at the last observation hour, namely the 114th hour.
Co-Authors Agustin, Azlinda Mitha Agustina, Eva AGUSTINA, EVA Aliyyah Nafiisah ANDYARINI, ESTI NOVI Auvaria, Shinfi Wazna Azlinda Mitha Agustin Dedy Suprayogi Esti Tyastirin Estri Kusumawati Eva Agustina Eva Agustina Eva Agustina, Eva Evi Fatimatur Rusydiyah Faidah, Nurul Ilmi Faizah, Hanik Ferdyanti, Anggun Putri Fiddaroini, Achmad Farchan Funsu Andiarna Hanik Faizah Heny Utami Ningsih Hernik Farisia Husniyatus Salamah Zainiyati Huwaidah, Jihan Farhah Ika Mustika Irul Hidayati Karim, Nuzulia Linda Prasetyaning Linda Prasetyaning W Linda Prasetyaning Widayanti Ma'arif, Muhammad Mei Lina Fitri Kumalasari Mei Lina Fitri Kumalasari Misbakhul Munir Moch. Irfan Hadi Mudalianah, Siti Muhammad Falikhul M Nabila, Salsa Nafiisah, Aliyyah Nafisah, Raihana Frika Nova Lusiana Nurul Ilmi Faidah P, Ria Indria Putri Darmansyah, Alifia Qurrotul Ayun Rahayu, Lusy Ria Qadariah Arief Rony Irawanto Rony Irawanto Rony Irawanto Salsa Nabila Salsa Nabila Sarita Oktorina Shofiyya, Ainina Neihayatus Siti Mudalianah Sri Hidayati Sri Hidayati L. Sri Mulyati Supardi Tania, Clarista Eka Ulfa Wafirotul Bahiyyah