Claim Missing Document
Check
Articles

Found 32 Documents
Search

Effectiveness and Role of Symbiotic Culture of Bacteria and Yeast (SCOBY) Kombucha Gel on Incisional Wound Healing in Mice (Mus musculus) Nafisah, Raihana Frika; Agustina, Eva; Purnamasari, Risa; Andiarna, Funsu; Hidayati, Irul
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2025): October 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.11282

Abstract

Incision wounds are common occurrences in daily life, and appropriate wound management is essential to promote and accelerate the healing process. Wound care can be performed using topical preparations such as gels containing bioactive compounds. SCOBY kombucha is believed to contain bioactive compounds, such as compounds from green tea (especially polyphenols) and metabolites produced by microbes (organic acids, alcohols, vitamins, etc.) The aim of this research is to determine the effectiveness of SCOBY kombucha gel on the healing of incision wounds in mice (Mus musculus). The method used in this research is a Completely Randomized Design (CRD) with 8 treatment groups, including 2 control groups and 6 test groups. The concentrations of SCOBY kombucha gel used are 0%, 2%, 4%, 8%, 10%, and 12%. The SCOBY kombucha gel is applied twice daily until the wound closes. Observation of the incision wound is carried out by measuring the length of the wound that has not yet closed. Based on the conducted research, it was found that SCOBY kombucha gel is effective in healing incision wounds as evidenced by the reduction in wound size due to the bioactive compounds in SCOBY. The most significant wound healing effect was observed with the 12% SCOBY kombucha gel, as there was a significant difference compared to the negative control group, 0%, 2%, 4%, and 8% SCOBY kombucha gels.
PENGUATAN KETERAMPILAN ABAD 21 GURU MIPA DALAM PEMBELAJARAN MIPA: TINJAUAN 4C, HOTS, DAN TPACK P, Ria Indria; Mulyati, Sri; Rahayu, Lusy; Purnamasari, Risa; Karim, Nuzulia
STRATEGY : Jurnal Inovasi Strategi dan Model Pembelajaran Vol. 6 No. 1 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/strategi.v6i1.8537

Abstract

Contemporary educational transformation requires educators, particularly in Mathematics and Natural Sciences (MIPA), to master competencies that are adaptive to global changes, technological innovation, and the learning needs of the digital generation. Twenty-first-century competencies encompass critical thinking, creativity, collaboration, and communication (4C), Higher Order Thinking Skills (HOTS), and Technological Pedagogical Content Knowledge (TPACK), which are considered fundamental in creating meaningful MIPA learning. The purpose of this article is to analyze the strategic importance of developing twenty-first-century competencies for MIPA educators within the Indonesian education system. A literature review approach was employed to examine research publications and educational regulations published between 2020 and 2025. The findings indicate a disparity between the expectations of twenty-first-century learning and the actual capacity of MIPA educators, particularly in facilitating HOTS and implementing pedagogy-based technology. The implementation of the Merdeka Curriculum, character building through the Pancasila Student Profile, and the performance of Indonesian students in science and mathematics literacy in global assessments such as PISA strengthen the argument for the importance of enhancing MIPA educators' capacity in twenty-first-century skills. This article proposes continuous professional development, educator communities of practice, and technology optimization through the TPACK framework as primary approaches to competency enhancement. ABSTRAK Transformasi pendidikan kontemporer mengharuskan pendidik, terutama dalam bidang Matematika dan Ilmu Pengetahuan Alam (MIPA), menguasai kompetensi yang adaptif terhadap perubahan global, inovasi teknologi, dan kebutuhan pembelajaran generasi digital. Kompetensi abad ke-21 mencakup berpikir kritis (critical thinking), kreativitas (creativity), kolaborasi (collaboration), komunikasi (communication) atau disingkat 4C, keterampilan berpikir tingkat tinggi (Higher Order Thinking Skills/HOTS), dan pengetahuan teknologi pedagogi konten (Technological Pedagogical Content Knowledge/TPACK) yang dianggap fundamental dalam menciptakan pembelajaran MIPA bermakna. Tujuan penulisan artikel ini ialah menganalisis kepentingan strategis pengembangan kompetensi abad ke-21 bagi pendidik MIPA dalam sistem pendidikan Indonesia. Pendekatan kajian literatur digunakan untuk menelaah publikasi riset dan regulasi pendidikan yang terbit antara tahun 2020 hingga 2025. Temuan mengindikasikan adanya disparitas antara ekspektasi pembelajaran abad ke-21 dengan kapasitas riil pendidik MIPA, terutama pada aspek fasilitasi HOTS dan penerapan teknologi berbasis pedagogi. Pelaksanaan Kurikulum Merdeka, pembentukan karakter melalui Profil Pelajar Pancasila, dan performa literasi sains serta matematika siswa Indonesia dalam evaluasi global seperti PISA memperkuat argumentasi pentingnya peningkatan kapasitas pendidik MIPA dalam keterampilan abad ke-21. Artikel ini mengusulkan pengembangan keprofesian berkelanjutan, komunitas praktik pendidik, dan optimalisasi teknologi melalui kerangka TPACK sebagai pendekatan utama dalam peningkatan kompetensi.
Application of Symbiotic Culture of Bacteria and Yeast Kombucha Gel on Incision and Burn Wounds in Mice (Mus musculus): Aplikasi Gel Symbiotic Culture of Bacteria dan Yeast Kombucha pada Luka Sayat dan Bakar Mencit (Mus musculus) Andiarna, Funsu; Hidayati, Irul; Agustina, Eva; Purnamasari, Risa
JRST (Jurnal Riset Sains dan Teknologi) Volume 10 No. 1, March 2026: JRST
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/jrst.v10i1.24079

Abstract

SCOBY (Symbiotic Culture of Bacteria and Yeast) is a result of kombucha tea fermentation that contains active compounds, such as phenolic acids and flavonoids, which possess high antioxidant activity. However, the use of SCOBY is currently limited to serving as a starter culture for new batches of kombucha tea, necessitating innovation to enhance its other potential benefits. This study aims to evaluate the effectiveness of kombucha SCOBY gel in the healing of incised and burn wounds in mice (Mus musculus). The research methods included the preparation of kombucha tea, harvesting of SCOBY, formulation of gel, application of the gel to incised and burn wounds in mice, and observation of wound healing until closure. The results showed that kombucha SCOBY gel significantly accelerated the healing of both incised and burn wounds compared to the control group. Incised wounds fully healed by day 6, while burn wounds healed by day 7. This effectiveness is associated with the high total phenolic content (TPC) of the SCOBY gel, measured at 790.4 mg GAE/g extract, along with strong antioxidant activity of 12.42% (DPPH assay). Statistical analysis revealed a significant difference (p<0.05) in wound healing between the untreated and SCOBY gel-treated groups for both types of wounds. Therefore, kombucha SCOBY gel has potential as an effective natural topical wound-healing agent, particularly for mild to moderate wounds.   ABSTRAK (Bahasa Indonesia) SCOBY (Symbiotic Culture of Bacteria and Yeast) merupakan hasil fermentasi teh kombucha yang mengandung senyawa aktif, seperti asam fenolat dan flavonoid, yang memiliki aktivitas antioksidan tinggi. Namun penggunaan SCOBY hanya terbatas sebagai starter dalam pembuatan teh kombucha baru, sehingga diperlukan inovasi untuk meningkatkan manfaat lain dari SCOBY. Tujuan penelitian ini adalah untuk mengevaluasi efektivitas gel SCOBY kombucha dalam penyembuhan luka sayat dan luka bakar pada mencit (Mus musculus). Metode penelitian ini dimulai dari pembuatan teh kombucha, pemanenan SCOBY, pembuatan gel pengaplikasian pada luka sayat dan bakar pada mencit dan pengamatan penyembuhan luka sampai penutupan luka. Hasil penelitian menunjukkan bahwa gel SCOBY kombucha mempercepat penyembuhan luka sayat dan bakar secara signifikan dibandingkan kelompok kontrol. Luka sayat sembuh sempurna pada hari ke‑6, sedangkan luka bakar sembuh pada hari ke‑7. Efektivitas ini dikaitkan dengan tingginya kadar total fenolik (TPC) gel SCOBY, yaitu sebesar 790,4 mg GAE/g ekstrak, serta aktivitas antioksidan kuat sebesar 12,42% (uji DPPH). Hasil analisis statistik menyatakan pada luka sayat dan bakar terdapat perbedaan signifikan(p<0,05) antara kelompok tanpa perlakuan dan gel SCOBY. Dengan demikian, gel SCOBY kombucha berpotensi sebagai agen penyembuh luka topikal alami yang efektif, khususnya untuk luka ringan hingga sedang.
Polisakarida Krestin dari Jamur Coriolus versicolor terhadap hitung Jenis Leukosit Mencit yang diinfeksi Mycobacterium tuberculosis Risa Purnamasari
Biotropic : The Journal of Tropical Biology Vol. 1 No. 2 (2017): Biotropic, Volume 1, Nomor 2, 2017
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2017.1.2.16-30

Abstract

Penelitian ini bertujuan untuk mengetahui peran polisakarida krestin (PSK) dengan waktu pemberian yang berbeda terhadap hitung jenis leukosit mencit yang diinfeksi Mycobacterium tuberculosis. Penelitian ini menggunakan 30 ekor mencit betina dewasa jenis Mus musculus strain BALB/C, berumur 8-10 minggu, berat badan berkisar 25-30 g. Polisakarida krestin (PSK) diisolasi dari Coriolus versicolor yang diperoleh dari alam. Infeksi menggunakan Mycobacterium tuberculosis strain H37Rv (ATCC 27294 T). Hewan percobaan dikelompokkan menjadi 6 kelompok sebagai berikut: kelompok I, hanya diberi akuades; kelompok II, hanya pemberian PSK; kelompok III, hanya dengan infeksi Mycobacterium tuberculosis; kelompok IV, pemberian PSK sebelum infeksi Mycobacterium tuberculosis; kelompok V, pemberian PSK sesudah infeksi Mycobacterium tuberculosis; kelompok VI, pemberian PSK sebelum dan sesudah infeksi Mycobacterium tuberculosis. Pemberian PSK dilakukan selama 7 hari berturut-turut melalui gavage. Infeksi Mycobacterium tuberculosis dilakukan sebanyak 2 kali dengan selang waktu 1 minggu melalui intraperitoneal. Hitung jenis leukosit dilakukan dengan mengelompokan masing-masing jenis leukosit dalam 100 sel leukosit pada apusan darah, dan data hasil pengamatan dianalisis dengan uji Kruskal-Wallis, kemudian untuk mengetahui signifikansi dilanjutkan dengan uji Mann-Whitney. Secara keseluruhan penelitian menunjukkan presentase jenis leukosit dengan jumlah tertinggi adalah neutrofil. Pada kelompok VI presentase monosit dan neutrofil meningkat melebihi normal, sedangkan presentase limfosit menurun, dan presentase basofil dan eosinofil tidak mengalami perubahan. Kesimpulan penelitian ini adalah PSK meningkatkan jumlah leuksosit mencit jenis neutrofil dan monosit pada waktu sebelum dan sesudah infeksi Mycobacterium tuberculosis
Efektivitas Mikronutrien Zinc dari Ekstrak Labu Kuning (Cucurbita Moschata D) Terhadap Perilaku Kawin Mencit (Mus musculus) Jantan Linda Prasetyaning; Risa Purnamasari; Nova Lusiana
Biotropic : The Journal of Tropical Biology Vol. 2 No. 1 (2018): Biotropic, Volume 2, Nomor 1, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2018.2.1.53-60

Abstract

Male reproductive ability ability has a linear correlation with libido and testosterone. Both are associated with various things main food consumption such as protein, vitamins and minerals. Aphrodisiac is an ingredient or drug that arouses sexual arousal or libido. Pumpkin is an example of food from plants with the highest content of zinc. This study was to determine whether there is a difference between the content of zinc on the peel, flesh and pumpkin seeds against sexual activity of mice. The experimental animals used were 24 male, group I mice as control group, Group II was grouped with pumpkin peel extract, Group III was grouped with extract of pumpkin flesh, Group IV was grouped with extract of pumpkin seeds. The dose used is 2gr / kg BB and volume of 0.2 ml is given for 35 days. Sexual Activity is observed every day by the position of male mice riding females. Based on Anova One Way test results showed there are differences in sexual activity mice males in each group (ρ 0.000). The difference of the result of the number of mounts indicated that the giving of yellow pumpkin extract had an effect on the increase of mating behavior of male mice. The group treated with the extract of pumpkin flesh showed the highest number of mice mounts (mean = 24,1667.Further research is needed to get a clearer picture of the composition of the pumpkin nutrients that indirectly affect fertility in men.
Pengaruh Pemberian Ekstrak Buah Pisang (Musa acuminate L) dan Buah Alpukat (Persea americana Mill) Terhadap Berat Hati Mencit (Mus musculus) Betina Heny Utami Ningsih; Ulfa Wafirotul Bahiyyah; Risa Purnamasari
Biotropic : The Journal of Tropical Biology Vol. 2 No. 1 (2018): Biotropic, Volume 2, Nomor 1, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2018.2.1.61-66

Abstract

Male reproductive ability ability has a linear correlation with libido and testosterone. Both are associated with various things main food consumption such as protein, vitamins and minerals. Aphrodisiac is an ingredient or drug that arouses sexual arousal or libido. Pumpkin is an example of food from plants with the highest content of zinc. This study was to determine whether there is a difference between the content of zinc on the peel, flesh and pumpkin seeds against sexual activity of mice. The experimental animals used were 24 male, group I mice as control group, Group II was grouped with pumpkin peel extract, Group III was grouped with extract of pumpkin flesh, Group IV was grouped with extract of pumpkin seeds. The dose used is 2gr / kg BB and volume of 0.2 ml is given for 35 days. Sexual Activity is observed every day by the position of male mice riding females. Based on Anova One Way test results showed there are differences in sexual activity mice males in each group (ρ 0.000). The difference of the result of the number of mounts indicated that the giving of yellow pumpkin extract had an effect on the increase of mating behavior of male mice. The group treated with the extract of pumpkin flesh showed the highest number of mice mounts (mean = 24,1667.Further research is needed to get a clearer picture of the composition of the pumpkin nutrients that indirectly affect fertility in men.
Analisis Kadar Glukosa, Hemoglobin, dan Kolesterol Mencit (Mus Musculus) Setelah Diinduksi Aspartam Esti Tyastirin; Risa Purnamasari; Irul Hidayati; Eva Agustina; Moch. Irfan Hadi; Nova Lusiana; Mei Lina Fitri Kumalasari
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2018.2.2.119-125

Abstract

Aspartame has a lower calorie rating than other sweeteners. for example, cyclamate, lactose, saccharin, fructose and maltosa. Although it has low calorie, aspartame have 60 times sweeter than sugar (sucrose). Induction of aspartame can cause elevated levels of blood glucose and damage to the pancreatic β cells of mice. The purpose of this study was to analyze the dosing of aspartame levels; 13 mg/Kg body weight, 39 mg/Kg and 78 mg/Kg and those effect to the levels of glucose, cholesterol and haemoglobin of female mice (Mus musculus). The methode of research laboratory experiments using a complete randomized design (RAL). This study was using 24 mice with 4 treatment groups. Mice were given aspartame during surgical treatment, mice were made to perform measurement of Hb levels, glucose levels and cholesterol levels of neonatal mice. Data analysis using the Kruskal Wallis because Gaussian data but not homogeneous. The results showed an increase in the levels of glucose and cholesterol levels, but statistically there is no difference in glucose levels (p = 0,087), Hb (p = 0,899) and cholesterol (p = 0,168) in mice (Mus musculus) who were given variations of the dose of aspartame.
Identifikasi Senyawa Aktif dari Ekstrak Daun Jambu Air (Syzygium aqueum) dengan Perbandingan Beberapa Pelarut pada Metode Maserasi Eva Agustina; Funsu Andiarna; Nova Lusiana; Risa Purnamasari; Moch. Irfan Hadi
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2018.2.2.108-118

Abstract

Daun jambu air (Syzygium aqueum) dikenal sebagai salah satu obat tradisional, untuk menyembuhkan beberapa infeksi akibat antigen berupa bakteri. Penelitian ini bertujuan untuk mengetahui kandungan senyawa aktif yang terdapat dalam daun jambu air dengan beberapa pelarut pada proses maserasi. Metode ekstraksi yang digunakan dalam penelitian ini adalah ekstraksi dingin berupa maserasi dengan pemilihan pelarut yakni metanol (CH3OH), etil asetat dan n-heksana. Uji fitokimia yang dilakukan adalah uji saponin, flavonoid, alkaloid dan triterpenoid/steroid. Dari uji yang dilakukan diketahui sampel ekstrak daun jambu air dengan pelarut metanol positif mengandung beberapa senyawa aktif berupa flavonoid, saponin, alkaloid dan triterpenoid. Ekstrak daun jambu air dengan pelarut etil asetat dan n-heksana positif mengandung beberapa senyawa aktif berupa flavonoid, alkaloid dan triterpenoid. Pelarut yang paling bagus untuk mendapatkan banyak senyawa aktif adalah metanol. Kata kunci : Jambu air (Syzygium aqueum), maserasi, senyawa aktif
Analisis Kariometric Akibat Pemberian Variasi Dosis Aspartam pada Perkembangan Fetus Mencit (Mus Musculus L.) Esti Novi Andyarini; Nova Lusiana; Ika Mustika; Estri Kusumawati; Sri Hidayati; Moch Irfan Hadi; Risa Purnamasari; Dedy Suprayogi
Biotropic : The Journal of Tropical Biology Vol. 3 No. 2 (2019): Biotropic, Volume 3 Nomor 2, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2019.3.2.86-95

Abstract

Aspartame is safe for consumption according to the prescribed ADI, but this artificial sweetener is not nutritious or does not contain calories so it is not recommended to be consumed during pregnancy. This study was to analyze cariometric effect of aspartame doses in the development of fetal mice (Mus muculus L.). The type of research used was experimental laboratory research on 24 adult mice with 4 treatment groups, control, aspartame dose 13 mg / Kg BB, 39 mg / Kg BB and 78 mg / Kg BB. Data was analyzed using SPSS 16 with one way ANOVA. Based on the results of the study there were changes in the number and weight of the fetus due to aspartame doses, there was no change in fetal length due of aspartame dose variations. Consumption of aspartame during pregnancy should be avoided because it can cause teratogenic effects. For further research, it is necessary to observe the morphology and histology of the fetus which is expressed by aspartame to determine the types of congenital abnormalities.
Pengaruh Pemberian Ekstrak Daging Buah Kurma Ajwa (phoenix dactylifera) Terhadap Hitung Jenis Leukosit Embrio dan Induk Mencit (mus musculus) Bunting Eva Agustina; Nova Lusiana; Risa Purnamasari
Biotropic : The Journal of Tropical Biology Vol. 3 No. 2 (2019): Biotropic, Volume 3 Nomor 2, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2019.3.2.135-145

Abstract

Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak daging buah Kurma Ajwa (Phoenix dactylifera) terhadap hitung jenis leukosit embrio dan induk mencit (Mus musculus) bunting. Setiap perlakuan dibagi dalam empat kelompok perlakuan ekstrak. Kelompok I (kelompok kontrol), kelompok II (3 kurma setara 3,12 mg/kg BB mencit), kelompok III (5 kurma setara 5, 2 mg/kg BB mencit), dan kelompok IV (7 kurma setara 7, 28 mg/kg BB mencit). Ekstrak diberikan pada masa kebuntingan 14 sampai 18 hari melalui peroral. Pada hari ke 19 kebuntingan dilakukan pembedahan untuk diambil darahnya pada bagian jantung mencit. Hitung jenis leukosit melalui teknik apusan darah dengan mengamati 100 sel leukosit perbesaran 400 kali pada mikroskop. Hasilnya ekstrak daging buah Kurma Ajwa (P. dactylifera) berpengaruh signifikan terhadap hitung limfosit dan basofil, tetapi tidak berpengaruh terhadap hitung monosit, eosinofil dan neutrofil pada embrio mencit. Sedangkan pada induk mencit berpengaruh terhadap hitung eosinofil dan monosit dan tidak berpengaruh terhadap limfosit, basofil dan neutrofil. Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak daging buah Kurma Ajwa (Phoenix dactylifera) terhadap hitung jenis leukosit embrio dan induk mencit (Mus musculus) bunting. Setiap perlakuan dibagi dalam empat kelompok perlakuan ekstrak. Kelompok I (kelompok kontrol), kelompok II (3 kurma setara 3,12 mg/kg BB mencit), kelompok III (5 kurma setara 5, 2 mg/kg BB mencit), dan kelompok IV (7 kurma setara 7, 28 mg/kg BB mencit). Ekstrak diberikan pada masa kebuntingan 14 sampai 18 hari melalui peroral. Pada hari ke 19 kebuntingan dilakukan pembedahan untuk diambil darahnya pada bagian jantung mencit. Hitung jenis leukosit melalui teknik apusan darah dengan mengamati 100 sel leukosit perbesaran 400 kali pada mikroskop. Hasilnya ekstrak daging buah Kurma Ajwa (P. dactylifera) berpengaruh signifikan terhadap hitung limfosit dan basofil, tetapi tidak berpengaruh terhadap hitung monosit, eosinofil dan neutrofil pada embrio mencit. Sedangkan pada induk mencit berpengaruh terhadap hitung eosinofil dan monosit dan tidak berpengaruh terhadap limfosit, basofil dan neutrofil.