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All Journal International Journal of Public Health Science (IJPHS) Jurnal Teknologi Laboratorium THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Analis Medika Biosains (JAMBS) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY JPP Jurnal Kesehatan Poltekkes Palembang Jaringan Laboratorium Medis Medica Arteriana (Med-Art) CENDEKIA : Jurnal Ilmu Pengetahuan Devotion: Journal of Research and Community Service IBTIDA : Jurnal Kajian Pendidikan Dasar HEALTHY: Jurnal Inovasi Riset Ilmu Kesehatan Sewagati Analis Kesehatan Sains (e-Journal) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Health Dynamics
Suhariyadi Suhariyadi
Departement Of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Perbandingan Pemeriksaan Skrining Antibodi (Indirect Coombs Test) dengan Direct Coombs Test pada Pasien dengan Riwayat Transfusi Darah Berulang Arta, Shela Yuni; Suhariyadi, Suhariyadi; Woelansari, Evy Diah; Rahayuningsih, Christ Kartika
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13643

Abstract

Repeated blood transfusions can increase risk of alloimmunization and autoimunization, which causes formation alloantibodies and autoantibodies. The presence of these antibodies can make cross-matching more difficult and increase the possibility of hemolytic transfusion risk. Antibody screening tests (Indirect Coombs Test) and Direct Coombs Test play a role in detecting the presence of alloantibodies and autoantibodies in patient with a history of repeated transfusion. The aim of the study was to determine the diferences in results between antibody screening (Indirect Coombs Test) and Direct Coombs Test in patients receiving repeated blood transfusions. This study used a quasi-experimental design with a cross-sectional approach. The subjects were patients with a history of blood transfusions more than 5 times at RSUD R.T. Notopuro Sidoarjo totalling 60 samples. The data obtained were then analyzed statistically to see if there were any significant differences between the two types of examinations. The results obtained showed a significant difference between the results of the antibody screening test (ICT) and the Direct Coombs Test (DCT) (p-value 0.000), that more patients with repeated blood transfusions had positive DCT results (75%). Some patients showed positive results on one or both types of tests, indicating the presence of alloantibodies or autoantibodies. ICT and DCT tests provide complementary information in detecting antibodies in patients with repeated transfusions. The use of both is important in improving the safety of blood transfusions and minimizing the risk of hemolytic reactions.
Co-Authors ., Pestariati Abror, Yogi Khoirul Alifiah, Salsabiil Rochman Aliya Ayu Rizqiyah Alizah, Nur Andreas Putro Ragil Santoso, Andreas Putro Ragil Anggraini, Anita Dwi Ariyani, Ahna Fitri Arta, Shela Yuni Asryadin, Asryadin Eko, Sugeng Endarini, Lully Hanni Evy Diah Woelansari Handayati, Anik Istanto, Wisnu istiqomah istiqomah Istiqomah Istiqomah Juliana Christyaningsih Krihariyani, Dwi Kumahartati, Intan Luthfi Rusyadi Mamik Mamik Minarti Minarti Museyaroh, Museyaroh Mutiarawati, Diah Titik Niswata, Navila Arfianti Novikasari, Caturizkyana Octarina, Nofita Rahayuningsih, Christ Kartika Rahayuningsih, Christ. Kartika Rahma Yuliana, Putri Rizqiyah, Aliya Ayu Sasongkowati, Retno Siti Helmyati Suliati Suliati Syamsul Arifin Uswatun Hasanah Widiastutik, Dyah Yuniati, Nilasari Indah