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All Journal International Journal of Public Health Science (IJPHS) Jurnal Teknologi Laboratorium THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Analis Medika Biosains (JAMBS) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY JPP Jurnal Kesehatan Poltekkes Palembang Medica Arteriana (Med-Art) CENDEKIA : Jurnal Ilmu Pengetahuan Devotion: Journal of Research and Community Service IBTIDA : Jurnal Kajian Pendidikan Dasar HEALTHY: Jurnal Inovasi Riset Ilmu Kesehatan Sewagati Analis Kesehatan Sains (e-Journal) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Health Dynamics
Suhariyadi Suhariyadi
Departement Of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Kolesterol dan Tekanan Darah Lansia di Posyandu Abiyoso Polkesbaya Christyaningsih, Juliana; Rusyadi, Luthfi; Minarti, Minarti; Mamik, Mamik; Pestariati, Pestariati; Mutiarawati, Diah Titik; Suliati, Suliati; Sasongkowati, Retno; Woelansari, Evy Diah; Suhariyadi, Suhariyadi; Istanto, Wisnu; Endarini, Lully Hanni; Museyaroh, Museyaroh
JURNAL RAKAT SEHAT (JRS) : Pengabdian Kepada Masyarakat Vol 2 No 2 (2023): Jurnal Rakat Sehat: Pengabdian Kepada Masyarakat
Publisher : UPPM Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jrs.v2i2.35

Abstract

As people age, the physical state of the human body will gradually deteriorate due to a decrease in the quantity of cells present, leading to a gradual reduction in functionality referred to as the aging process. In elderly humans (aged more than 60 years) excessive cholesterol levels and high blood pressure are one of the causes of diseases such as heart disease which has been recognized by WHO as the number one cause of death in the world. The buildup of fatty deposits on the linings of blood vessels has the potential to lead to obstructions within the blood vessels. The objective of this initiative is to enhance understanding regarding the hazards associated with cholesterol. This outreach effort took place in July 2023, conducted at Posyandu Abiyoso, Surabaya Health Polytechnic. The activity consists of several processes 1). conducting a survey of the location of the Abiyoso posyandu of the Surabaya Health Polytechnic, 2). Opening registration and taking participants' personal data, 3) conducting participant health interviews, 4). Checking cholesterol levels and blood pressure of participants. From the community service activities carried out, it was found that 42 of the 65 (64.6%) participants who took part in the examination at the integrated service post had high cholesterol levels, and 60 of the 65 (92.3%) participants had high blood pressure or hypertension. By conducting health checks on cholesterol levels and blood pressure, there is an expectation that the society will come to realize and comprehend. the dangers of hypertension and hypercholesterolemia so that the community can prevent the occurrence of sustainable diseases.
Kecenderungan Nilai Hematokrit (HCT) pada Ibu Hamil Menggunakan Metode Fowcytometry Octarina, Nofita; Suhariyadi, Suhariyadi; Arifin, Syamsul
Medica Arteriana (Med-Art) Vol 6, No 2 (2024): Desember 2024
Publisher : University of Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/medart.6.2.2024.56-62

Abstract

Latar Belakang: Angka kejadian anemia pada ibu hamil trimester pertama sebesar 20%, trimester kedua sebesar 70%, dan trimester ketiga sebesar 70%. Penyebab anemia selama hamil yaitu peningkatan volume plasma yang lebih besar dibandingkan dengan peningkatan sel darah merah sehingga menyebabkan hemodilusi (pengenceran). Apabila volume plasma terus meningkat dan tidak diimbangi peningkatan produksi eritropoietin dapat menyebabkan penurunan kadar hematokrit. Penurunan nilai hematokrit cenderung rendah pada ibu hamil trimester kedua dan ketiga. Salah satu pemeriksaan laboratorium untuk mengetahui derajat anemia adalah pemeriksaan hematokrit. Penelitian ini bertujuan untuk mengukur nilai hematokrit pada ibu hamil trimester pertama, kedua, dan ketiga menggunakan metode flowcytometry dan menganalisis nilai hematokrit pada ibu hamil trimester pertama, kedua, dan ketiga.Metode: Penelitian ini bersifat deskriptif dengan sampel penelitian yaitu data primer dari pemeriksaan sampel darah vena pasien ibu hamil trimester pertama, kedua, dan ketiga yang datang ke Puskesmas Gedangan, Sidoarjo. Teknik pengambilan sampel menggunakan total sampling sebanyak 30 orang ibu hamil. Metode yang digunakan adalah flowcytometry dengan alat Hematology Analyzer. Analisa data menggunakan uji distribusi frekuensi. Penelitian dilakukan di Laboratorium Puskesmas Gedangan, Sidoarjo, Jawa Timur pada bulan Februari 2024.Hasil: Hasil penelitian nilai hematokrit dari 30 sampel ibu hamil didapatkan hasil dengan nilai normal sebanyak 12 orang (40%) dan di bawah normal sebanyak 18 orang (60%).Kesimpulan: Nilai hematokrit ibu hamil di Puskesmas Gedangan, Sidoarjo, Jawa Timur cenderung rendah dan mengalami anemia.
Pemberdayaan Kader Kesehatan Dan Keluarga Pasien Tuberkulosis Dalam Pemanfaatan Sambiloto Sebagai Imunomodulator Untuk Keberhasilan Pengobatan Pasien Tuberkulosis Di Wilayah Kerja Puskesmas Gading Kecamatan Tambaksari Kota Surabaya Pestariati, Pestariati; Handayati, Anik; Suhariyadi, Suhariyadi
Sewagati Vol 9 No 3 (2025)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v9i3.2276

Abstract

Indonesia memiliki beban kasus tuberkulosis tertinggi ketiga di dunia. Tahun 2021, jumlah kasus tuberkulosis di Indonesia 824.000 kasus, hanya 402.502 kasus (49%) dari target 85% berhasil ditemukan dan diobati . Indikator keberhasilan pengob- atan di Jawa Timur tahun 2020 sebesar 87,1% (Target renstra Kemenkes 90%). Hal ini disebabkan salah satunya rendahnya pemahaman kader kesehatan, keluarga dan pasien tuberculosis tentang penyakitnya, termasuk bahaya resistensi OAT dan dukun- gan keluarga terhadap kesembuhan pasien. Sambiloto memiliki kandungan senyawa andrografolida, sebagai imunomodulator, meningkatkan keberhasilan pengobatan TB. Kader kesehatan berperan mengedukasi masyarakat tentang TB, memberikan dukungan dan motivasi kepada pasien TB,memfasilitasi kolaborasi antara pasien TB. Setiap Kelurahan Wilayah Puskesmas Gading memiliki Kader Kesehatan yang khusus menangani TB Pada Kelurahan di wilayah Puskesmas Gading, warga memi- liki tanaman Sambiloto baik dilakukan di Pot atau masuk di dalam TOGA RT. Pengabdian memberikan edukasi tentang tuberkulosis dan pemanfaatan sambiloto sebagai imunomodulator kepada kader kesehatan /TB dan keluarga pasien TB. Pen- ingkatan pemahaman kader dan keluarga pasien TB tentang sambiloto (N-Gain > 0,7) berhasil menurunkan angka BTA positif dari 84% menjadi 37,5%, sehingga edukasi (KIE) berkelanjutan sangat diperlukan.
KORELASI LEUKOSIT DAN IGM ANTI-SALMONELLA SEBAGAI PENUNJANG DIAGNOSIS PASIEN SUSPEK DEMAM TIFOID Ariyani, Ahna Fitri; Krihariyani, Dwi; Istanto, Wisnu; Suhariyadi, Suhariyadi
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 4 No. 2 (2025)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v4i2.5826

Abstract

ABSTRACT Typhoid fever is an infectious disease that remains prevalent in Indonesia and often presents with non-specific clinical symptoms, necessitating supportive diagnostic examinations. Two commonly used parameters are leukocyte count and anti-Salmonella typhi IgM serological testing using the rapid test method. This study aims to determine the correlation between leukocyte count and the results of the anti-Salmonella IgM rapid test in patients suspected of having typhoid fever as a diagnostic aid. This research employed an analytical observational method with a cross-sectional approach. A total of 32 out of 45 suspected typhoid fever patients were selected through purposive sampling at RSUD Dr. Sayidiman Magetan. Leukocyte counts were measured using an automated hematology analyzer, and IgM testing was performed using the immunochromatographic rapid test method. The results showed that 59.4% of patients had leukopenia, and only 21.9% tested positive for IgM. Spearman’s rank correlation test yielded a significance value (p) = 0.027 and a correlation coefficient (r) = 0.391, indicating a moderate and statistically significant correlation between the two variables. In conclusion, there is a significant positive correlation between leukocyte count and anti-Salmonella IgM results, suggesting that both tests can serve as complementary diagnostic tools for the early diagnosis of typhoid fever. ABSTRAK Demam tifoid merupakan penyakit infeksi yang masih sering dijumpai di Indonesia dan menimbulkan gejala klinis yang tidak spesifik, sehingga diperlukan pemeriksaan penunjang untuk membantu diagnosis. Dua parameter yang umum digunakan adalah jumlah leukosit dan uji serologis IgM anti-Salmonella typhi dengan metode rapid test. Penelitian ini bertujuan untuk mengetahui korelasi antara jumlah leukosit dan hasil rapid test IgM anti-Salmonella pada pasien suspek demam tifoid sebagai penunjang diagnosis. Penelitian ini menggunakan metode observasional analitik dengan pendekatan cross-sectional. Sampel sebanyak 32 pasien dari 45 pasien suspek demam tifoid yang diambil di RSUD Dr. Sayidiman Magetan secara purposive sampling. Pemeriksaan jumlah leukosit dilakukan dengan alat hematologi otomatis dan uji IgM dilakukan menggunakan metode rapid test imunokromatografi. Hasil penelitian menunjukkan bahwa 59,4% pasien mengalami leukopenia dan hanya 21,9% pasien yang menunjukkan hasil IgM positif. Uji korelasi Rank Spearman menunjukkan nilai signifikansi (p) = 0,027 dan koefisien korelasi (r) = 0,391, yang menunjukkan adanya hubungan yang cukup kuat dan signifikan secara statistik antara kedua variabel. Kesimpulannya, terdapat hubungan positif yang signifikan antara jumlah leukosit dan hasil IgM anti-Salmonella, sehingga keduanya dapat digunakan sebagai pemeriksaan penunjang yang saling melengkapi dalam diagnosis awal demam tifoid.
Correlation Of Neutrophil Lymphocyte Ratio (NLR) Value With Incidence Of Tuberculosis Disease In Patients With Diabetes Mellitus Istiqomah, Istiqomah; Suhariyadi, Suhariyadi; Woelansari, Evy Diah
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.332

Abstract

Diabetes mellitus is a non-communicable disease (PTM) that is chronic and can weaken the immune system so that sufferers are 3 times more likely to suffer from pulmonary TB. In chronic infection there is continuous inflammation which is characterized by the presence of neutrophils themselves and will be followed by low lymphocyte values due to immune system pathways that do not work perfectly. The purpose of this study was to determine the correlation between the Neutrophil Lymphocyte Ratio (NLR) and the incidence of Tuberculosis in Diabetes Mellitus patients at the Jorongan Health Center, Probolinggo Regency. This research is a correlative analytic study with a cross sectional approach. A total of 30 samples of diabetes mellitus patients at the Jorongan Health Center from January to April 2023. The NLR examination method was with a hematology analyzer and tuberculosis patients were examined using Molecular Rapid Test. The statistical test uses Spearman's nonparametric test. The results showed that there was a correlation between NLR values and the incidence of tuberculosis in diabetes mellitus patients at the Jorongan Health Center, p = 0.003 and r = 0.521. Further research needs to be carried out involving larger samples and other laboratory tests related to the immune system in patients with diabetes mellitus.
Korelasi Kadar High Sensitivity C-Reactive Protein (hs-CRP) Dan Kolesterol Low Density Lipoprotein (LDL) Pada Perokok Aktif Dengan Aktivitas Fisik Rizqiyah, Aliya Ayu; Woelansari, Evy Diah; Suhariyadi, Suhariyadi
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.14363

Abstract

Jombang Regency was the 10th most prevalent smoker in East Java in 2018 and experienced an increase in the prevalence of smokers by 0.1% from 2013. The high number of smokers raises a concern. Smoking may lead to an increase of hs-CRP and LDL cholesterol levels, where the increase in these two parameters is closely related to the formation of atherosclerotic plaques, as a cause of cardiovascular disease. Physical activity of each individual is something that needs to be known because it affects the levels of hs-CRP and LDL cholesterol. This study aims to analyze the correlation between hs-CRP and LDL cholesterol in active smokers with physical activity. This study used an observational analytic study with a cross-sectional design. This research was conducted in March-April 2022 with a total sample of 32 active smokers who work as pedicab drivers at Pondok Pesantren 'DU', Jombang Regency. The hs-CRP examination was carried out using the Latex Turbidimetric Assay method, while the LDL cholesterol examination was using the Homogeneous Enzymatic Colorimetric Assay method. Based on the statistical analysis of the Spearman correlation, the results obtained p>0.05 (p = 0.687, r = 0.074), which means that there is no correlation between levels of hs-CRP and LDL cholesterol in active smokers with physical activity.
PERBANDINGAN STATUS ANTI-HBs (Hepatitis B surface Antibody) DAN JUMLAH LIMFOSIT PADA PESERTA PROGRAM VAKSINASI HEPATITIS B DENGAN DAN TANPA KOMORBID DIABETES MELITUS TIPE 2 Widiastutik, Dyah; Suhariyadi, Suhariyadi; Rahayuningsih, Christ. Kartika; Woelansari, Evy Diah
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 4 No. 4 (2025)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v4i4.6582

Abstract

Anti-HBs and lymphocyte count are used to assess the success of hepatitis B vaccination. Healthcare workers are at high risk of contracting hepatitis B, making routine vaccination essential. This study was conducted at RSUD Caruban, Madiun Regency, involving 44 participants of the hepatitis B vaccination program, both with and without type 2 diabetes mellitus comorbidity. Examinations were carried out six months post-vaccination. The study employed a comparative analytical method with a cross-sectional approach and was conducted in February 2025. The instruments used were the Eclia-Mindray CL-900i with the ELISA method and the Sysmex XN-350 with the Flow Cytometry method. In the group with type 2 diabetes mellitus, the average Anti-HBs titer was 77.53 mIU/mL, whereas in the non-diabetic group it was 882.06 mIU/mL. The average lymphocyte count was also higher in the non-diabetic group (38.91%) compared to the group with diabetes mellitus (22.52%), although both were within the normal range. There were differences in Anti-HBs status and lymphocyte counts between hepatitis B vaccination participants with and without type 2 diabetes mellitus comorbidity. ABSTRAKAnti-HBs dan jumlah limfosit digunakan untuk menilai keberhasilan vaksinasi hepatitis B. Tenaga kesehatan memiliki risiko tinggi tertular hepatitis B, sehingga vaksinasi rutin diperlukan. Penelitian ini dilakukan di RSUD Caruban Kabupaten Madiun, pada 44 peserta vaksinasi hepatitis B, baik dengan maupun tanpa komorbid diabetes melitus tipe 2. Pemeriksaan dilakukan enam bulan setelah vaksinasi. Penelitian ini menggunakan metode komparatif analitik dengan pendekatan cross-sectional, dilaksanakan pada bulan Februari tahun 2025. Instrumen yang digunakan adalah Eclia-Mindray CL-900i, dengan metode ELISA dan Sysmex XN-350, dengan metode Flow Cytometry. Pada kelompok dengan diabetes melitus tipe 2, rata-rata titer Anti-HBs adalah  77,53 mIU/mL, sementara kelompok tanpa diabetes melitus rata-ratanya adalah 882,06 mIU/mL. Untuk jumlah limfosit rata-ratanya lebih tinggi pada kelompok tanpa diabetes melitus yaitu sebesar 38,91 % dibandingkan kelompok dengan komorbid diabetes melitus tipe 2 sebesar 22,52%, meskipun keduanya dalam batas normal. Terdapat perbedaan status Anti-HBs dan jumlah limfosit pada peserta program vaksinasi hepatitis B dengan dan tanpa komorbid diabetes melitus tipe 2.
HUBUNGAN JUMLAH SEL LEUKOSIT MONONUKLEAR (MONOSIT, LIMFOSIT) DENGAN HASIL UJI TUBEX TF TERHADAP PASIEN SUSPEK DEMAM TIFOID Kumahartati, Intan; Woelansari, Evy Diah; Istanto, Wisnu; Suhariyadi, Suhariyadi
CENDEKIA: Jurnal Ilmu Pengetahuan Vol. 5 No. 4 (2025)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/cendekia.v5i4.7143

Abstract

Typhoid fever can be diagnosed in suspected patients through the Tubex TF test, which has a relatively high sensitivity and specificity of 73%. Typhoid fever can also be diagnosed by examining the number of mononuclear leukocytes, particularly monocytes and lymphocytes, which are indicators of bacterial infection. Typhoid fever is a disease caused by Salmonella typhi. Typhoid fever cases in Indonesia range from 350 to 810 per 100,000 people, with a prevalence of 1.6%. The purpose of this study was to determine the relationship between the number of mononuclear leukocytes (monocytes and lymphocytes) and Tubex TF results in patients suspected of typhoid fever. This study was an analytical observational study and the sampling technique used was consecutive sampling, with all subjects meeting the selection criteria, amounting to 63 samples. The number of mononuclear leukocytes (monocytes, lymphocytes) was determined using a Hematology Analyzer and the Tubex TF test was determined using the IMBI method. Spearman's correlation test was used for statistical analysis. The findings showed a significant correlation between the number of monocytes and the Tubex TF results in patients suspected of typhoid fever (p = 0.006 < ? = 0.05). In contrast, no significant correlation was found between the number of lymphocytes and the Tubex TF results in the same group of patients (p = 0.296 > ? = 0.05). ABSTRAKDiagnosis demam tifoid pada pasien suspek dapat dilakukan melalui uji Tubex TF yang menunjukkan sensitivitas dan spesifisitas yang relative tinggi, yaitu sebesar 73%. Demam tifoid dapat pula didiagnosis dengan pemeriksaan jumlah sel leukosit mononuklear, khususnya sel monosit dan sel limfosit yang akan menjadi indikator ketika terjadi infeksi akibat bakteri. Demam tifoid adalah penyakit yang disebabkan oleh infeksi bakteri Salmonella typhi. Kasus demam tifoid di Indonesia berkisar antara 350 hingga 810 per 100.000 penduduk, dengan prevalensi 1,6%. Tujuan penelitian ini, untuk mengetahui hubungan jumlah sel leukosit mononuklear (monosit, limfosit) dengan hasil Tubex TF pada pasien suspek demam tifoid. Penelitian ini merupakan penelitian observasional analitik dan juga teknik pengambilan sampelnya ialah dengan cara Consecutive Sampling yaitu semua subyek yang memenuhi kriteria pemilihan yaitu sebanyak 63 sampel. Jumlah sel leukosti mononuclear (monosit, limfosit) ditentukan dengan alat Hematology Analyzer dan uji Tubex TF ditentukan dengan metode IMBI. Uji korelasi Spearman digunakan untuk analisis statistik. Temuan menunjukkan adanya hubungan yang signifikan antara jumlah monosit dan hasil Tubex TF pada pasien yang diduga demam tifoid (p = 0,006 < ? = 0,05). Sebaliknya, tidak ditemukan korelasi yang signifikan antara jumlah limfosit dan hasil Tubex TF pada kelompok pasien yang sama (p = 0,296 > ? = 0,05).
Comparison of Platelet Index in Type 2 Diabetes Mellitus Patients With and Without Diabetic Ulcers Rahma Yuliana, Putri; Suhariyadi, Suhariyadi; Woelansari, Evy Diah
Devotion : Journal of Research and Community Service Vol. 6 No. 11 (2025): Devotion: Journal of Community Research
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/devotion.v6i11.25573

Abstract

Hyperglycaemia is caused by poor insulin production and insulin resistance, which is the beginning of type 2 diabetes mellitus. The blockage of blood vessels caused by this disease is due to the increase in blood sugar levels, which is the cause of diabetic ulcers. As a result, patients are often unaware of their injuries. This research aims to identify differences in platelet indices (MPV, PDW and PCT) in people with type 2 diabetes mellitus who have diabetic ulcers and those who do not. This research uses observational analysis methods and a cross-sectional analysis approach. The independent variable in this study was type 2 diabetes mellitus patients with and without diabetic ulcers, while the dependent variable in this study was the platelet value, consisting of MPV in type 2 diabetes mellitus patients with and without diabetic ulcers of 7,716 fL and 7,837 fL. The mean PCT in type 2 diabetes mellitus patients with and without diabetic ulcers was 0.225% and 0.245%, respectively. The mean PDW in type 2 diabetes mellitus patients with and without diabetic ulcers was 18.825% and 14.237%, respectively. The results of data analysis using unpaired t-test showed a p-value of 0.00 for PDW and 0.002 for MPV, so it was concluded that there were differences in MPV and PDW in type 2 diabetes mellitus patients who had diabetic ulcers and those who did not.
Development of a Rapid Diagnostic Method for Simultaneous Detection of Streptococcus viridans in Cases of Heart Disease Pestariati, Pestariati; Suhariyadi, Suhariyadi; Asryadin, Asryadin; Yuniati, Nilasari Indah
Jurnal Penelitian Pendidikan IPA Vol 11 No 11 (2025): November: In Progress
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i11.12730

Abstract

Heart disease is a leading cause of death worldwide, with a complex pathophysiology often involving interactions between genetic factors, the environment, and pathogenic microorganisms. Streptococcus viridans is a clinically significant pathogenic bacterium associated with infections of the cardiovascular system, including infective endocarditis, pericarditis, and other complications. However, timely and accurate diagnosis of this bacterial infection in cases of acute heart disease is often challenging, requiring rapid and sensitive diagnostic methods. Currently, diagnostic methods for detecting Streptococcus viridans in cases of acute heart disease tend to be time-consuming; therefore, developing a rapid diagnostic method that can detect both bacteria simultaneously is crucial. The aim of this study is to develop a rapid and sensitive diagnostic method for detecting the presence of Streptococcus viridans in samples from patients with heart disease. The method used is the identification of specific genes, the design of primer sequences, and the design of probes using specific 16s rRNA genes using bioinformatics techniques. Based on the research results obtained primer pair sequences are: oligonucleotide primer forward 5’-GCGACGATACATAGCCGAC-3’; primer reverse is 3’- CGAGCCAGTCTGAAAGC-5’, while the probe sequence is 5’-GACTGAGACACGGCCCAGACTC-3’. Primer and probe pair quality tests showed very good primer and probe quality for amplification with a 120 bp amplification product. Suggestions in the research are that it is necessary to continue with qPCR optimization to determine the melting temperature which is then carried out sensitivity tests of primer pair sequences and specific 16s rRNA Streptococcus viridans gene probes.
Co-Authors ., Pestariati Abror, Yogi Khoirul Alifiah, Salsabiil Rochman Aliya Ayu Rizqiyah Alizah, Nur Andreas Putro Ragil Santoso, Andreas Putro Ragil Anggraini, Anita Dwi Ariyani, Ahna Fitri Asryadin, Asryadin Eko, Sugeng Endarini, Lully Hanni Evy Diah Woelansari Handayati, Anik Istanto, Wisnu Istiqomah Istiqomah istiqomah istiqomah Juliana Christyaningsih Krihariyani, Dwi Kumahartati, Intan Luthfi Rusyadi Mamik Mamik Minarti Minarti Museyaroh, Museyaroh Mutiarawati, Diah Titik Niswata, Navila Arfianti Novikasari, Caturizkyana Octarina, Nofita Rahayuningsih, Christ. Kartika Rahma Yuliana, Putri Rizqiyah, Aliya Ayu Sasongkowati, Retno Siti Helmyati Suliati Suliati Syamsul Arifin Uswatun Hasanah Widiastutik, Dyah Yuniati, Nilasari Indah