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Induksi Organogenesis pada Mikropropagasi Anggrek Vanda tricolor Lindl. serta Deteksi Variasi Genetik Hasil Perbanyakan dengan RAPD RINDANG DWIYANI
Agrotrop : Journal on Agriculture Science Vol 6 No 2 (2016)
Publisher : Fakultas Pertanian Universitas Udayana

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Induction of Organogenesis on Micropropagation of Vanda tricolor Orchidand Detection of Genetic Variation of the Progenies Using RAPD Marker.Research concerning on micropropagation of Vanda tricolor orchid and detection of geneticvariation among the progenies has been conducted in the year of 2015. The objective of theresearch was to obtain the most appropriate explant type for inducing organogenesis and toobserve genetic variation of the progenies using Random Amplification of Polymorphic DNA(RAPD). Various pieces of organ from 18 month old-seedlings were used as material ofmicropropagation, i.e. shoot tip, shoot base, leaf tip and leaf base. Explants were planted onMS medium without plant growth regulator. The percentage of explant producing shoots andthe average number of shoots per-explant were observed at 12 weeks after planting. Progeniesresulted from the micropropagation were then analysed using RAPD with 5 primers, i.e.OPA8 (5’ GTGACGTAGG 3’), OPA9 (5’ GGGTAACGCC 3’), OPA10 (5’ GTGATCGCAG3’), OPA11 (5’ CAATCGCCGT 3’), OPA13 (5’ CAGCACCCAC 3’). The results showedthat the most appropriate explant for inducing organogenesis was shoot base. Analysis ofRAPD showed that the slight variation was detected between mother plant and the progeniesand also among the progenies. The results obtained from the current research are promisingand suggests that RAPD markers can be utilized as a simple molecular tool to assess thegenetic variation of plants derived in-vitro.
Pengaruh Penambahan Ca(NO3)2 Terhadap Hasil Tanaman Selada Kriting (Lactuca Sativa L.) pada Sistem Hidroponik Deep Flow Technique (DFT) NI NYOMAN SURYANTINI; GEDE WIJANA; RINDANG DWIYANI
Agrotrop : Journal on Agriculture Science Vol 10 No 2 (2020)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2020.v10.i02.p08

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The Effect of Addition of Ca(NO3)2 to the Results of Lettuce (Lactuca sativa L.) in the Hydroponic System of Deep Flow Technique (DFT). Plants, in general, require composition, concentration, and volume of nutrient solutions which different depends on the type and phase of plant growth. Leaf vegetable crops such as lettuce (Lactuca sativa L.) require higher Nitrogen (N) nutrients than other vegetable crops, but the problem we faced at the moment is the hydroponic nutrients that are traded only in the general form of nutrient, therefore the addition of N nutrients in the form of calcium nitrate (Ca(NO3)2) needs to be done to meet the needs of lettuce plants. This study was aimed to determine the effect of the addition of Ca(NO3)2 and to find the optimum dosage of the addition of Ca(NO3)2 to the general hydroponic nutrition of vegetables on lettuce use DFT hydroponic system. The treatments consisted of five levels which are nutrition AB Mix general vegetable as a control, and nutrition AB Mix with the addition of 30 g, 60 g, 90 g, and 120 g Ca(NO3)2. The results of the observation showed that the treatment of the addition of Ca(NO3)2 fertilizer had a very significant effect on all observed variables, except for the diameter of the lettuce plant. The optimum addition of Ca(NO3)2 for lettuce was found at 90.04 g with a maximum yield of 207.92 g.
Transformasi Gen Pembungaan melalui Agrobacterium tumefaciens Secara In-Vitro pada Tanaman Anggrek Vanda tricolor RINDANG DWIYANI; HESTIN YUSWANTI; IXORA SARTIKA MERCURIANI; ENDANG SEMIARTI
Agrotrop : Journal on Agriculture Science Vol 6 No 1 (2016)
Publisher : Fakultas Pertanian Universitas Udayana

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In Vitro Transformation of Flowering Gene through Agrobacterium tumefaciens on Vandatricolor Orchid. Research concerning of Agrobacterium-mediated transformation of Vanda tricolor orchid had been done during March to August 2014. Objectives of this research wasto investigate the effects of period of inoculation with Agrobacterium suspension and application of acetosyringone (AS) on the percentage of transforman candidates. The T-DNA harbored the PaFT gene under the control of promoter of Ubiquitin and the hpt gene, ahygromycine resistant gene as a selectable marker for transformant selection. This T-DNA was constructed in the PGAS vector and transferred to the plant genome mediated by A. tumefaciens. The period of inoculation with Agrobacterium and application ofAcetosyringone (AS) were trialed in this research. The result showed that a period of one hour inoculation with Agrobacterium suspension added with 25 ppm AS resulted in the highest number of transforman candidates. i.e. 7.04% compared to 4.10% (one hour inoculation without AS), 3.76% (two hours inoculation without AS), and 4.48% (two hours inoculation with 25 ppm AS).
Perbaikan Kualitas Buah Anggur Bali (Vitis Vinifera L. Var. Alphonso Lavallee) melalui Aplikasi GA3 sebelum Bunga Mekar I NYOMAN GEDE ASTAWA; NI NYOMAN ARI MAYADEWI; I MADE SUKEWIJAYA; NI LUH MADE PRADNYAWATHI; RINDANG DWIYANI
Agrotrop : Journal on Agriculture Science Vol 5 No 1 (2015)
Publisher : Fakultas Pertanian Universitas Udayana

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Improvement of The Quality of Balinese Table grape (Vitis Vinifera L. Var. Alphonso Lavallee) using GA3 Application Before Flowering. Research concerning of “Improvement of Balinese Tablegrape (Vitis Vinifera L. Var. Alphonso Lavallee) using GA3 before flowering” had been done during May to August 2015 at the vineyard located at Kalianget Village, in Buleleng Regency, Bali. Theobjective of the research was to find out method in improving the quality of Balinese table grape using GA3 application. The experiment was designed as Randomized Block Designed, with GA3concentration as a treatment, i.e. 0, 50, 100, 150, 200 ppm. The results showed that, application of GA3 before flowering increased bunch length, improved oBrix, induced seedless, however decreasedberry weight.
Induksi Kalus dengan 2,4-Dpada Mikropropagasi Tanaman Stroberi (Fragraria x ananassa Duch cv. Rosalinda) I.A. PUTRI DARMAWATI; RINDANG DWIYANI; HESTIN YUSWANTI
Agrotrop : Journal on Agriculture Science Vol 3 No 2 (2013)
Publisher : Fakultas Pertanian Universitas Udayana

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Callus Induction with Aplication of 2,4-D in Micropropagation of Strowberry(Fragraria xananassaDuch cv. Rosalinda). Strawberryis grown and well planted in many countries and has ahigh economic value. In Bali,thecentraproduction is in Pancasari Village,Sukasada District,BulelengRegency. The limited number and quality of seedling are the problems currently faced bystrawberry farmers at Pancasari Village. Seedlings produced by conventional propagation are limitedand are not able to meet the needs of seedling available for planting area. This study offers a solution tothe problem by applyingmicropropagationtechnique through callus induction. The research aim is tofind the optimal concentration of 2,4 - D to induce callus of strowberry. Callus that forms will be usedas the material for the next growing into young plants regenerated seedlings. This experiment used acompletely randomized design with 2 factors. The first factor was theconcentrations of 2,4 - D withfive levels of treatments, i.e.0 ; 0.5, 1.0, 1.5, and 2.0 ppm and the second factor was type of explantsused, including tip , middle and base of leaf. Results showed that2,4-D concentration of 1 and 2 ppmapplied on the base of the leaf is able to induce 100 % callus at 14 days after planting. Callus formedwas greenish white. The callus will be regeneratedinto plantlets or young plants.
Pertumbuhan dan Hasil Berbagai Varietas Kacang Hijau (Vigna radiata (L.) Wilczek) pada Kadar Air yang Berbeda YUDHANI WIDHYA HARTIWI; GEDE WIJANA; RINDANG DWIYANI
Agrotrop : Journal on Agriculture Science Vol 7 No 2 (2017)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.771 KB) | DOI: 10.24843/AJoAS.2017.v07.i02.p03

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Growth and Yield of Various Mung Bean Varieties (Vigna radiata (L.) Wickzek in Different Water Content. Mung bean (Vigna radiata (L.) Wilczek) is a commodity crop beans are commonly grown on dryland and high protein. Problems encountered in cultivation of mung bean in dry land is low results, one of them the limited seed of drought stress tolerant therefore needs to be done against drought stress research production of mung beans. Objective to know the growth and the results of various varieties of mung beans at different water levels. This experiment using Random Design complete with two factors. The treatment consists of the water content of the soil with a capacity of 100% airy, 75%, 50%, 25% and varieties of Fore Belu, Kutilang, Vima-1, and Sriti. The results of the experiment demonstrated the interaction between moisture content and varieties against the number of pods per plant, number of seeds per plant dry weight, dry seeds per plant, seeds of oven dry weight per plant dry weight and the oven 100 seeds. Moisture content of the soil with a roomy capacity (100%) gives the results of dry weight per plant seeds under the highest (17.2 g) compared to other treatments. Varieties that produce the highest growth found in the varieties of Sriti. Moisture content of the soil with a capacity of 100% airy gives the best results on the plant mung beans. Most varieties are tolerant of most soil water content lower than the capacity of the airy is Sriti varieties.
Respon Pertumbuhan Bibit Anggrek Dendrobium sp. pada Saat Aklimatisasi terhadap Beragam Frekuensi Pemberian Pupuk Daun RINDANG DWIYANI
Agrotrop : Journal on Agriculture Science Vol 2 No 2 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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The aim of the experiment was to investigate the appropriate frequency of foliar fertilizer applicationof Hyponex and Gandasil D for the growth of seedlings of Dendrobium sp. at acclimatization stage.Various frequency of application for both foliar fertilizers were trialed, i.e. once in 5 days, once in 10days, once in 15 days and control (without fertilizer). The results showed that the most appropriatefrequency for the application of Hyponex and Gandasil D for seedlings of Dendrobium sp. was once inevery 10 days.
Respon Sporofit Paku Ata (Lygodium circinnatum (Burn. F) Swartz) terhadap Pemberian Pupuk Urea RINDANG DWIYANI; HESTIN YUSWANTI
Agrotrop : Journal on Agriculture Science Vol 2 No 1 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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Lygodium circinnatum (Burn.f.) Swartz is a wild Pteridophyta which is used as a material for handicraft. Recently, Lygodium has been rare in nature due to overgathering. Domestication of this wild plant may required to overcome this problem. A research concerning domestication of Lygodium was conducted at Faculty of Agriculture of Udayana University during March to October 2009. Dose of urea fertilizer in varied levels was applied to 8 month-old sphorophyte. The level of urea doses were 0, 100, 200, 300 and 400 mg urea per plant. The results showed that urea fertilizer significantly stimulated the number of “sulur” (“sulur” is an organ of Lygodium that used as material for handicraft) , but did not affect the number of leaves and plant height. Dose of 300mg urea per plant resulted in the highest number of the average of “sulur” number per plant.
Pengaruh Kombinasi Naphthalene Acetic Acid (NAA) –Benzyl Amino Purine (BAP) dan Jenis Eksplan pada Mikropropagasi Anggrek Vanda tricolor Lindl. var. suavis AYU INDAH WIDAYANTI; RINDANG DWIYANI; HESTIN YUSWANTI
Agrotrop : Journal on Agriculture Science Vol 4 No 1 (2014)
Publisher : Fakultas Pertanian Universitas Udayana

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The Effect of Combination of BAP-NAA and Explant Types on Micropropagation of Orchidof Vanda tricolor Lindl. var. suavisThe objectives of the research was to investigate the effect ofcombination of NAA–BAP, type of explant and their interactions on shoot initiation in micropropagationof Vanda tricolor L. var. suavis. The factorial experiment consisted of two factors that was arrangedby completely randomized design (CRD) with three replications. The first factor was combination ofNAA-BAP i.e. M0= MS medium without any plant growth regulator; M1= MS + 0,1 mgL-1 NAA +0 mgL-1 BAP; M2= MS + 0,1 mgL-1 NAA + 0,1 mgL-1 BAP; M3= MS + 0,1 mgL-1 NAA + 1 mgL-1 BAP ; and M4= MS + 0,1 mgL-1 NAA + 10 mgL-1 BAP. The second factor was explant type i.e.shoot tip (B1) and shoot base (B2). The results showed that there was no interaction between factorsfor all variable observed. Medium M0 (MS medium without any plat growth regulator) and explant ofB1 (shoot tip) gave the highest number of the percentage of explants producing shoots and the highestnumber of shoots per explant.
Pengaruh 2-iP dan NAA terhadap Pertumbuhan Plantlet Anggrek Dendrobium Hibrida pada Tahap Subkultur ADINDA RIZKI NURANA; GEDE WIJANA; RINDANG DWIYANI
Agrotrop : Journal on Agriculture Science Vol 7 No 2 (2017)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.217 KB) | DOI: 10.24843/AJoAS.2017.v07.i02.p05

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The Effects of 2-iP and NAA on The Growth of Dendrobium Hybrid Plantlets of in Subculture. The research was conducted during period of May to October 2016 at The Laboratory of Plant Tissue Culture, Faculty of Agriculture, Udayana University. The research employed a completely randomized design with 9 treatments and 3 replicates. The treatment were ZA = MS + 1 ppm 2-iP + 0,00 ppm NAA; ZB = MS + 1 ppm 2-iP + 0,25 ppm NAA; ZC = MS + 1 ppm 2-iP + 0,50 ppm NAA; ZD = MS + 2 ppm 2-iP + 0,00 ppm NAA; ZE = MS + 2 ppm 2-iP + 0,25 ppm NAA; ZF = MS + 2 ppm 2-iP + 0,50 ppm NAA; ZG = MS + 3 ppm 2-iP + 0,00 ppm NAA; ZH = MS + 3 ppm 2-iP + 0,25 ppm NAA; ZI = MS + 3 ppm 2-iP + 0,50 ppm NAA. The result showed that treatment of 2-iP and NAA significantly affected number of leaves, length of leaves, total of fresh weight and oven dry weight of plantlet. The concentration of 2 ppm 2-iP and 0,00 ppm NAA was the best treatment for optimal growth for Dendrobium plantlet during subculture.
Co-Authors ADINDA RIZKI NURANA AJENG IDVATUL FITROH ANAK AGUNG MADE ASTININGSIH Ari Indrianto Ari Indrianto Ari Indrianto AStiningsih, Ana Agung Made AYU INDAH WIDAYANTI Azis Purwantoro Azis Purwantoro Azis Purwantoro Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto BARAELA EZRA WIJAYA Bimantara, Putu Oki Cokorda Gede Alit Semarajaya Danga, Jeni Rambu Yaku DINI WIRDASARI EKA CHRISTY WAKANNO Endang Semiarti Endang Semiarti ENDANG SEMIARTI ENIEK KRISWIYANTI GEDE WIJANA Gusti Ngurah Alit Susanta Wirya H. Yuswanti H. Yuswanti HESTIN - YUSWATI HESTIN YUSWANTI I GEDE PUTU WIRAWAN I GUSTI AGUNG INDRA MERTAWAN I Gusti Alit Gunadi I KETUT ARSA WIJAYA I Ketut Suada I MADE CHRISTIAN ADHI SAPUTRA I Made Sudana I Made Sudana I Made Sudana I Made Sukewijaya I NYOMAN GEDE ASTAWA I NYOMAN RAI I Putu Wahyu Sanjaya I WAYAN RUMADA I.A. MAYUN I.G.A. Gunadi I.N.G. Astawa IDA AYU ASTARINI Ida Ayu Putri Darmawanti Ida Ayu Putri Darmawati IDA AYU PUTRI DARMAWATI Ida Ayu Sri Dewi Adriani Ida Bagus Komang Mahardika Indrianto, A - Ixora Sartika Mercuriani K.A. Yuliadhi K.B. Susrusa KADEK PEBRIYANI KETUT BUDI SUSRUSA KETUT SUADA Ketut Suada LUH PUTU SUGIARI M. Sudiana Mahendra MADE SRITAMIN Manullang, Prila Kartika Muhammad Rivai N.N.A. Mayadewi N.N.A. Mayadewi NI LUH MADE PRADNYAWATHI NI LUH MARTINI NI NYOMAN ARI MAYADEWI NI NYOMAN SURYANTINI Ni Putu Ayu Erninda Oktaviani Suputri Pradana, I Kadek Wira Purba, Reza Stefany Purwantoro, A - Putu Suwardike Redana, I Made Basma Retno Kawuri Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sanjaya, I Putu Wahyu Semiarti, E. - SONDANG RAJAGUKGUK TRISNA AGUNG PHABIOLA UTAMI - - Y. Fitriani YUDHANI WIDHYA HARTIWI Yuyun Fitriani