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All Journal Jurnal Hortikultura Indonesia (JHI) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Agrotrop : Journal on Agriculture Science AGRITROP International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Jurnal Biologi Udayana Bumi Lestari E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Bionatura AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology Caraka Tani: Journal of Sustainable Agriculture ANNALES BOGORIENSES Agro Bali: Agricultural Journal Jurnal Agrotropika Nandur

Rindang Dwiyani

Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana, Jimbaran Bali

Author-ID : 256963

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Medicine & Pharmacology

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Agrobacterium tumefaciens-MEDIATED IN PLANTA TRANSFORMATION METHOD FOR THE SoSPS1 GENE IN CITRUS PLANTS (Citrus nobilis L.) Ni Putu Ayu Erninda Oktaviani Suputri; Rindang Dwiyani; Ida Ayu Putri Darmawanti; Bambang Sugiharto
International Journal of Biosciences and Biotechnology Vol 7 No 1 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

The SoSPS1 gene of sugar cane plants previously subjected to Agrobacterium tumefacienmediated cloning was to be transferred to citrus plants to increase metabolism of sucrose in plant. The T-DNA harbored the SoSPS1 gene under the control of the CaMV 35S promoter from the cauliflower mosaic virus and contained the NPTII gene (kanamycin resistance gene) as a selectable marker for transformant selection. Generally, gene transformation in plants is carried out by tissue culture. However, tissue culture has several disadvantages such as its being time-consuming, its sometimes resulting in somatic mutations and somaclonal variations, and the requirement of sterile conditions in the procedure of gene transfer. In planta transformation is a useful system for those plants that lack tissue culture and regeneration system. The main function of in planta transformation is to recover the advantages of tissue culture as an efficient, quick method, including its ability to produce a large number of transgenic plants and to accumulate a high concentration of total soluble protein in short time. There are two procedures of in planta transformation for the seeds of citrus plants, namely “prick and coat” and “seed tip-cutting and imbibition”. In the prick and coat method, seeds are pricked on their entire surfaces and smeared with a suspension of Agrobacterium tumefaciens. In the seed tip-cutting and imbibition method, on the other hand, seeds are cut at the tip and soaked in a suspension of Agrobacterium tumefaciens. The leaves derived from seeds treatment were taken as samples for DNA extraction and PCR using primers of the NPTII gene (Forward: 5’-GTCATCTCACCTTCCTCCTGCC-3’; Reverse: 5’-GTCGCTTGGTCGGTCATTTCG-3’). This research found that only the seed tip-cutting and imbibition plants amplified along the 550-bp band, while those of the prick and coat method did not. Additionally, the T-DNA was successfully integrated into the genome of the plants treated with the seed tip-cutting and imbibition method but not with the prick and coat.

DNA POLYMORPHISM AND GENETIC DIVERSITY OF MANGO (Mangifera sp.) GERMPLASM IN TROPICAL ISLAND Putu Suwardike; I Nyoman Rai; Rindang Dwiyani; Eniek Kriswiyanti
International Journal of Biosciences and Biotechnology Vol 7 No 1 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Creation of new superior varieties of mango through the empowerment of local genetic resources requires information about the potential properties of mango germplasm, including DNA polymorphism and its genetic diversity. This research aimed to obtain basic data on DNA polymorphism, genetic relationship, genetic similarity level, and molecular accession of Bali’s unique local mango. Sampling was conducted in four regencies in the Province of Bali. DNA preparation, PCR, and microsatellite analysis were carried out at the Laboratory of Genetics and Plant Breeding, Faculty of Agriculture, Gadjah Mada University. It found 44accessions of mangoes in Bali. The test results with 10 pairs of SSR markers showed that all primers produced polymorphic loci. There were 825 amplified DNA bands. Primer of AY31 produced the highest number of loci, which were 14 loci, while AY21 produced the fewest loci, i.e., 3 loci. Forty-four mango accessions showed a genetic similarity coefficient of 0.27 to 0.97. At a coefficient of 0.27, accessions were divided into 2 major groups: group A and group B. Group A consisted of two accessions, namely, KRA-005 and BDG-006 (Mangifera foetida Lour.), which had a similar coefficient of 0.657. Group B was divided into twosmaller groups, namely, groups B1 and B2, at a similarity coefficient of 0.342. Group B1 consisted of 39 accessions, while group B2 consisted of 3 accessions. Accessions Madu Anggur, Gading, Sambuk Mengwi, Kakul and Pakel Sulangai were identified as having unique alleles.

IN-PLANTA TRANSFORMATION METHOD MEDIATED WITH Agrobacterium tumefaciens FOR T-DNA TRANSFER IN TABLE GRAPE (Vitis vinifera L.) Rindang Dwiyani; Hestin Yuswanti; Yuyun Fitriani; Bambang Sugiharto
International Journal of Biosciences and Biotechnology Vol 6 No 2 (2019)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

The aim of the research is to investigate a simple method of in planta transformation method for T-DNA transfer in table grape. The T-DNA harbored the S0SPS1 gene under the control of promoter of the 35S CaMV from the Cauliflower Mosaic Virus and contained the NPTII gene, a kanamycin-resistant gene as a selectable marker for transformant selection. Six-month plants originated from cuttings were used as target plants. We explored two methods of in planta transformation, namely ”dipping” and “sweeping”. For both methods, the leaves of the target plants were removed and those of shoots without leaves were used as the target of transformation. In the “dipping method”, those shoots were dipped with the agrobacterial suspension for 60 seconds. However, for the “sweeping method”, the scars (the spots where leaves were removed) were swept with agrobacterial suspension using a cotton bud. Those treated non-leafy-shoots (from both methods) then were grown to be leafy shoots. Those leafy shoots then were cut and transplanted into the soil and grown to be a whole plant. The leaves of those plants then were taken as samples for DNA extraction and PCR using primers of NPTII gene (Forward: 5’-GTCATCTCACCTTCCTCCTGCC-3’; Reverse: 5’ GTCGCTTGGTCGGTCATTTCG-3’) with expected amplified band of 550 bp. We found that only the “sweeping method” plants amplified the 550 bp bands, while those of the “dipping method” did not. We suggest that the T-DNA was successfully integrated into the genome of plants treated with the “sweeping method” but not with the “dipping method”. Leaf sugar content (oBrix) of PCR-positive vines was higher than those of the wild-type vines, ensuring the integration of the T-DNA into the plant genome.

GENETIC DIVERSITY OF GUAVA (Psidium Guajava L.) IN BALI INDONESIA BASED ON RAPD MARKER Ni Nyoman Ari Mayadewi; I Nyoman Rai; Rindang Dwiyani; Ida Ayu Astarini
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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The aim of this study was to investigate genetic diversity and relationship of guava (local name: jambu biji) genotypes grown in Bali, Indonesia, based on Random Amplified Polymorphic DNA (RAPD) markers. Twelve guava cultivars namely Jambu biji ‘Australia’, ‘Bangkok Merah’, ‘Bangkok Putih’, ‘Cokorde’, ‘Dadu 1’, ‘Dadu 2’, ‘Getas Merah’, ‘Kamboja’, ‘Kristal’, ‘Pipit’, ‘Susu’ and ‘Variegata’ were collected from nine Regencies in Bali using survey and explorative method. Ten decamers RAPD primers were employed to distinguish between the 12 cultivars and determine their genetic relationships. A dendogram was constructed using coefficient dissimilarity analysis based on phylogenetic analysis using parsimony (PAUP). The twelve cultivars were grouped into 2 main clusters and five smaller clusters. Variation between genotypes of guava local will be good sources for future crop improvemen.

PRICK AND SOAK Agroacterium tumefaciens-MEDIATED IN PLANTA TRANSFORMATION IN TOMATO (Lycopersicon esculentum Mill.) I Putu Wahyu Sanjaya; Rindang Dwiyani; I Gede Putu Wirawan; Bambang Sugiharto
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

One of the modern plant breedings through genetic engineering is Agrobacterium tumefaciens-mediated transformation. Agrobacterium tumefaciens-mediated transformation can be performed in vitro or in planta. In planta transformation arises from the weaknesses of the in vitro method such as need high hygiene standard, professional tissue culture experts, and more time to prepare explants and somaclonal variation. In planta transformation is a method to transfer the gene to the plant genome without any tissue culture stages. The aims of this research were to know the possibility of the prick and soak in planta method with the target of tomato seeds and to know the most suitable inoculation time for tomato seeds transformation by prick and soak method the transformation is done by pricking the seeds and soaking them in the A. tumefaciens suspension. The treatments in this study were 1 and 2 days inoculation time to test the efficacy of prick and soak in planta transformation method. Tomato seeds were pricked with a needle on the center once, and then soaked in A. tumefaciens strain LB4404 suspension carrying pKYS-SoSPS1 plasmid with Neomycin Phosphotransferase (NPTII) and Saccharum officinarum Sucrose Phosphate synthase (SoSPS1) genes. Visualization of tomato’s DNA samples after PCR showed that 1-day inoculation sample was positively integrated with NPTII gene and negative in the 2 days inoculation treatment.

IN-VITRO INDUCTION OF GRAPEVINE (Vitis Vinifera L.) SHOOTS USING 2-iP Rindang Dwiyani
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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The research concerning induction of grapevine shoots grown in vitro have been conducted during period of May to July 2016 at The Laboratory of Plant Tissue Culture, Faculty of Agriculture, Udayana University. The objective of the research was to investigate the most appropriate explant position and the optimal concentration of 2-iP in stimulating of grapevine shoot in vitro. Nodal segment from grapevine plant grown in the green house was used as planting material. The experiment was laid out in the factorial design, with two factors. The first factor was the position of explant (lay-down and up-right position) and the second factor was concentration of 2-iP ( i.e. 0, 3, 6, 9, 12 ppm). Each combination was replicated ten times. The results showed that the treatment of 6 ppm 2-iP with up-right explant position was the most appropriate condition in stimulating growth of grapevine shoot. With up-right position of explant, the percentage of shoot producing-explants at 10 weeks after planting was 100% at the treatment of 6 ppm 2-iP, compared to 4%, 12%, 40%,and 40% at the treatment of 0 , 3, 9 and 12 ppm 2-iP respectively. The average number of shoot per explant was 5.5 for 6 ppm-treatment, while it was less than 3 for other treatments.

PELATIHAN BUDIDAYA JAMUR TIRAM DI DESA BELANGA KINTAMANI SUKEWIJAYA SUKEWIJAYA; I M., RINDANG DWIYANI; I.A. MAYUN; N.N. ARI MAYADEWI; COK. G.A. SEMARAJAYA
Buletin Udayana Mengabdi Vol 7 No 2 (2008): Volume 7 No.2 – September 2008
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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ABSTRACT Nowadays, demand of mushroom increase along with the changing of consume pattern which concerning on various and the quality of food, however, their supply relatively low. Low knowledge and skill of mushroom producer is one of limitation. Belanga rural community potentially develop cause of their natural resources, hence the knowledge and skill need to be improved by workshop. The workshop was covered the member farmer’s organization of Belanga (Subak Abian) by planting method and postharvest including their marketing. Field trip to mushroom producer and by monitoring and evaluating of workshop topic included to this workshop. At the present, the farmer was produced the oyster mushroom even if on small scale area. Confidently, by their improved skill of oyster mushroom farmers financial in Belanga may perhaps be enhanced.

Pelatihan Budidaya Kaktus Mini bagi Ibu-Ibu Anggota PKK Banjar Kerthabuana Kaja Denpasar Utara Rindang Dwiyani; Hestin Yuswanti; I Nyoman Gede Astawa; Ni Nyoman Ari Mayadewi
Buletin Udayana Mengabdi Vol 18 No 3 (2019): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Kaktus mini merupakan tanaman indoor penghias ruangan yang memiliki prospek baik untuk dikembangkan. Budidayanya yang sangat mudah tidak banyak diketahui orang sehingga perlu menyebarkan ilmu budidaya ini kepada masyarakat, khususnya kaum ibu rumah tangga. Ibu rumah tangga menghabiskan sebagian besar waktunya di rumah untuk mengurus keluarga, namun pemanfaatan waktu yang tersisa perlu dipikirkan dengan baik agar tidak terbuang percuma. Pelatihan budidaya kaktus mini untuk para ibu anggota PKK di Banjar Kerthabuwana Kaja ini telah dilaksanakan dengan baik. Kegiatan ini memberikan pengetahuan serta ketrampilan kepada para ibu sehingga mereka dapat melakukannya di rumah sebagai bagian dari hobi sekaligus mendatangkan uang. Metode yang digunakan adalah metode ceramah dan praktik langsung. Tujuan dari kegiatan ini adalah meningkatkan ketrampilan para ibu rumah tangga dalam budidaya tanaman kaktus mini. Output dari kegiatan ini adalah ketrampilan sederhana yang didapat oleh para ibu rumah tangga, yakni pembiakan kaktus mini hingga pemeliharaannya sampai siap dijual. Kata kunci: kaktus mini, budidaya, ibu PKK

PEMBUATAN BIBIT ANGGREK HIBRIDA DAN ADAPTASI PADA LINGKUNGANNYA MENGGUNAKAN METODE AKLIMATISASI R. Dwiyani; H. Yuswanti; I.N.G. Astawa; N.N.A. Mayadewi
Buletin Udayana Mengabdi Vol 20 No 1 (2021): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

The objective of the community services was to improve the skill of housewives and other people in making of hybrid orchids. The Training has been carried out at 24 August 2019 at The Field Station of Faculty of Agriculture, Udayana University, Denpasar. The participants were a group of housewives and some lecturers who will get their resign in the next few years. The outcome of the current community services is an improvement of the skill of participants in making orchid hybrids through artificial pollination and also skill in aclimatisation of bottled-orchid seedlings. This skill may increase their income because the products that have been sucseesfully made have a chance to be sold at agriculture event elsewhere or may in some nurseries that found in Denpasar. The output of this activity is an article that will be puplished at national journal; a brief guide in making hybrid orchids ( Indosesian: Teknologi tepat guna); and products i.e. orchid seedlings in pot and capsules of orchid that resulted from artificial pollination.

CALLUS INDUCTION ON BANANA FLOWER’S EXPLANT IN VITRO USING 2,4-DICHLOROPHENOXYACETIC (2,4-D) RINDANG - DWIYANI; HESTIN - YUSWATI; UTAMI - -
Jurnal Biologi Udayana Vol 22 No 2 (2018): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

ABSTRACT The objective of the study was to obtain the best 2,4-D concentration on callus induction of the banana flowers in banana propagation using indirect organogenesis method. Kesuna, local banana cultivar obtained from Sembung Gede, Tabanan was used as explant material. Callus induction was performed using 2,4-Dichlorophenoxyacetic acid with concentration of 0; 0.5; 1.0; 1.5 and 2.0 ppm. Each treatment was represented by 3 bottles and each bottle was planted with 3 explants, so each treatment was represented by 9 explants of banana flowers. The results showed that the concentration of 2.0 ppm 2.4-D induced callus with the fastest time and gave the highest percentage of the explants producing callus. The calluses were subsequently subcultured into regeneration medium using 0.5 mg/L Benzylaminopurine (BAP) and 0.005 mg/L Napthaleneaceticacid (NAA). The calluses were subsequently sub-cultured into a regeneration medium using 0.5 ppm (BAP) and 0.005 ppm Naphthalene acetic acid (NAA) to induce shoots and roots and performed plantlets. Keywords: 2,4-Dichlorophenoxyacetic acid, banana’s flowers, callus

Co-Authors ADINDA RIZKI NURANA AJENG IDVATUL FITROH ANAK AGUNG MADE ASTININGSIH Ari Indrianto Ari Indrianto Ari Indrianto AStiningsih, Ana Agung Made AYU INDAH WIDAYANTI Azis Purwantoro Azis Purwantoro Azis Purwantoro Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto BARAELA EZRA WIJAYA Cokorda Gede Alit Semarajaya Danga, Jeni Rambu Yaku DINI WIRDASARI EKA CHRISTY WAKANNO Endang Semiarti ENDANG SEMIARTI Endang Semiarti ENIEK KRISWIYANTI GEDE WIJANA Gusti Ngurah Alit Susanta Wirya H. Yuswanti H. Yuswanti HESTIN - YUSWATI HESTIN YUSWANTI I GEDE PUTU WIRAWAN I GUSTI AGUNG INDRA MERTAWAN I Gusti Alit Gunadi I Kadek Wira Pradana I KETUT ARSA WIJAYA I Ketut Suada I MADE CHRISTIAN ADHI SAPUTRA I Made Sudana I Made Sudana I Made Sudana I Made Sukewijaya I NYOMAN GEDE ASTAWA I NYOMAN RAI I Putu Wahyu Sanjaya I WAYAN RUMADA I.A. MAYUN I.G.A. Gunadi I.N.G. Astawa IDA AYU ASTARINI Ida Ayu Putri Darmawanti Ida Ayu Putri Darmawati IDA AYU PUTRI DARMAWATI Ida Ayu Sri Dewi Adriani Ida Bagus Komang Mahardika Indrianto, A - Ixora Sartika Mercuriani K.A. Yuliadhi K.B. Susrusa KADEK PEBRIYANI KETUT BUDI SUSRUSA KETUT SUADA Ketut Suada LUH PUTU SUGIARI M. Sudiana Mahendra MADE SRITAMIN Manullang, Prila Kartika Muhammad Rivai N.N.A. Mayadewi N.N.A. Mayadewi NI LUH MADE PRADNYAWATHI NI LUH MARTINI NI NYOMAN ARI MAYADEWI NI NYOMAN SURYANTINI Ni Putu Ayu Erninda Oktaviani Suputri Purba, Reza Stefany Purwantoro, A - Putu Suwardike Retno Kawuri Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sanjaya, I Putu Wahyu Semiarti, E. - SONDANG RAJAGUKGUK TRISNA AGUNG PHABIOLA UTAMI - - Y. Fitriani YUDHANI WIDHYA HARTIWI Yuyun Fitriani

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