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All Journal Jurnal Hortikultura Indonesia (JHI) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Agrotrop : Journal on Agriculture Science AGRITROP International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Jurnal Biologi Udayana Bumi Lestari E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Bionatura AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology Caraka Tani: Journal of Sustainable Agriculture ANNALES BOGORIENSES Agro Bali: Agricultural Journal Jurnal Agrotropika Nandur
Rindang Dwiyani
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana, Jimbaran Bali
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Medicine & Pharmacology

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Pengaruh Penambahan Trichoderma Spp. pada Media Tanam Terhadap Pertumbuhan Bibit Pisang (Musa acuminata) Cavendish Hasil Kultur Jaringan I Kadek Wira Pradana; Rindang Dwiyani; Gusti Ngurah Alit Susanta Wirya
Agro Bali : Agricultural Journal Vol 7, No 1 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i1.1568

Abstract

Trichoderma merupakan salah satu mikroorganisme spesifik spesies, jadi masing-masing spesies Trichoderma akan memberikan pengaruh yang berbeda terhadap spesies tanaman yang berbeda. Penelitian ini dilakukan dengan tujuan untuk mengetahui Pengaruh Penambahan Trichoderma spp. Pada Media Tanam Terhadap Pertumbuhan Bibit Pisang (Musa acuminata) Cavendish Hasil Kultur Jaringan. Penelitian ini menggunakan rancangan percobaan satu faktor dengan rancangan lingkungan RAL. Trichoderma ditambahkan dengan perbedaan spesies Trichoderma yaitu T0 = Kontrol, Ta = T. asperellum., Th= T. harzianum, Tk = T. koningii, Tv = T. viride. Tahapan penelitian aklimatisasi plantlet tahap satu (3 minggu), inkubasi Trichoderma pada kompos (2 minggu), aklimatisasi tahap dua (6 minggu). Data yang diperoleh dari pengukuran, penghitungan dan pengamatan variabel dianalisis secara statistik dengan menggunakan analisis of variance (ANOVA). Hasil analisis ANOVA menunjukkan bahwa aplikasi Trichoderma spp. pada tanaman pisang (Musa Acuminata) cavendish hasil kultur in vitro berpengaruh tidak nyata P ≥ 0,05 terhadap semua variabel yang diamati. Namun ada kecenderungan pertambahan jumlah helai daun Th lebih banyak 1,75 helai dibanding T0, panjang akar Th lebih panjang 6,525 cm dibanding T0.
The Role of Mycorrhizae on the Growth of Banana Planlets of Cultivar Raja (Musa Paradisiaca C.V. Raja) from Bali at Post Acclimatization: Role of Mycorrhizae on the Growth of Banana Planlets Dwiyani, Rindang; Wirya, Gusti Ngurah Alit Susanta; Gunadi, I Gusti Alit; Darmawati, Ida Ayu Putri; Yuswanti, Hestin; Susrusa, Ketut Budi; AStiningsih, Ana Agung Made
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.11

Abstract

The effect of inoculation of a combination of the arbuscular mycorrhizal fungus of Glomus and Acaulospora on micropropagated banana plantlets cultivar Raja from Bali during the acclimatization phase was investigated. The experiment was conducted at the Experimental Station of the Faculty of Agriculture, Udayana University, Denpasar, Bali, Indonesia, over the period of March to June 2022. The objective of this research was to evaluate the impact of various doses of mycorrhizae on the growth of tissue culture banana plantlets that had undergone acclimatization for a period of six weeks. A total of five different doses of mycorrhiza were applied to the acclimatized plantlets, namely M0=without mycorrhiza and M5=5g mycorrhiza.kg-1, M10=10 g mycorrhiza.kg-1, M15=15 g mycorrhiza.kg-1, M20=20 g mycorrhiza.kg-1. A significant effect was discovered at M15; however, compared to M20, M20 gave better results for root length and fresh weight of the above-ground part, indicating the best dose was 20 g mycorrhiza kg-1. This study concluded that mycorrhiza has a very significant role in supporting the growth of micro propagated banana plantlets at post-acclimatization. Variables observed, such as the average number of leaves, stem diameter, above-ground plant fresh and dry weight, and root fresh and dry weight, increased with the application of AMF (Glomus and Acaulospora).
Pengaruh Pemberian GA3 dan Ekstrak Paku Sayur (Diplazium esculentum) terhadap Kualitas Hasil Buah Anggur (Vitis vinifera L.) Varietas Kediri Kuning Purba, Reza Stefany; Astawa, I Nyoman Gede; Dwiyani, Rindang
Agrotrop : Journal on Agriculture Science Vol 14 No 2 (2024)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2024.v14.i02.p14

Abstract

Effect of GA3 and Vegetable Spike Extract (Diplazium esculentum) on the Yield Quality of Grape (Vitis vinifera L.) Kediri Kuning Variety. This study aims to determine the effect of vegetable fern extract solution on the quality of grapes of Kediri Kuning variety and the concentration that can improve the quality of grapes. This research uses RAK (Randomized Group Design) with the treatment of vegetable fern extract consisting of 5 levels with 5 replicates, namely P0 = Control, PG = GA3 500 ppm, PS25 = 25%, PS50 = 50%, PS75 = 75%. The results showed that the treatment of vegetable fern extract solution gave an influence on the variables of titratable acid content, fruit bunch length, number of seeds per fruit, vitamin c content, antioxidant properties content, and seed weight per fruit, tannin content. Dipping the vegetable fern extract solution in the stadia before flower blossom can improve the quality of Kediri Kuning grapes, seen in titratable acid content, fruit bunch length, number of seeds per fruit, weight of seeds per fruit, vitamin C content, antioxidant properties, and tannin content. The concentration of 50% vegetable spike extract is able to provide the best results and quality to improve the quality of Kediri Kuning grapes.
Intraspecific SSR Marker Screening for Detection of Dendrobium crumenatum Mutants Generated from In Vitro Gamma Irradiation Dwiyani, Rindang; Sanjaya, I Putu Wahyu; Yuswanti, Hestin; Darmawati, Ida Ayu Putri; Suada, I Ketut; Manullang, Prila Kartika; Fitriani, Yuyun
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.89896

Abstract

Determination of D. crumenatum mutant obtained from in vitro mutation breeding needs a long time due to its long-life cycle. SSR molecular markers can be used for early mutant detection. Specific SSR markers developed in D. crumenatum are not yet available. Alternative published SSR markers were developed from D. catenatum. The aims of this study are to screen the most informative SSR markers generated from D. catenatum tested in irradiated D. crumenatum population and to determine the gamma irradiation dose resulting the most mutants. Ten SSR markers were randomly selected and tested in 25 individuals of D. crumenatum plantlets irradiated with several doses (0, 5, 10, 15, and 20 Gy; 5 plantlets each dose). The result showed 7 of 10 primers were polymorphic and the other three were  monomorphic. All seven polymorphic primers can be used to identified intraspecific variation in the D. crumenatum mutant population. Markers dnsr28 and dnsr98 were the most informative, with the highest polymorphic information content (PIC) value of 0.5. Irradiation D. crumenatum protocorms using 10-15 gray doses were detected as the highest mutant percentage obtained up to 100% in the sample tested. This resulting marker information can be used to screen wider mutant population to decrease the non-mutant individuals in the population for maintenance and cost efficiency. The 10-15 Gy can be used as reference doses for gamma irradiation in 3 months old D. crumenatum protocorm materials. 
Meristem Culture of Strawberry Plants (Fragaria x Ananassa Duch.) Sachinoka Variety on Various Types of Basic Media In Vitro Fitriani, Yuyun; Yuswanti, Hestin; Dwiyani, Rindang; Darmawati, Ida Ayu Putri
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p08

Abstract

The large number of in vitro media circulating in the market is a challenge to find the most effective media for sachinoka strawberry meristem culture, because the need for in vitro media depends on genetic intelligence. The purpose of this study was to find the best media from the various tested media to reproduce the Sachinoka variety strawberry seedlings in a relatively short time using the meristem culture method. This study used a completely randomized design (CRD) with 6 media treatments and repeated 7 times, namely: M1 (WPM without BA), M2 (WPM + 4 ppm BA), M3 (NP without BA), M3 (NP + 4 ppm BA), M5 (BMM without BA), M6 (BMM + 4 ppm BA). The variables observed were the time of emergence of shoots, the number of shoots and the number of leaves. The results of this study indicate that there is no significant difference for the emergence of shoots variable. Treatment M1 produced the highest number of shoots (12.29) but not significantly different from M5 (10.14), both were significantly different from both treatments. The highest number of leaves is in M1 (27.14). It can be concluded that the best medium for the growth of sachinoka strawberry meristem culture is WPM media with the addition of 4 ppm BA, while the second-best medium is BMM media with the addition of 4 ppm BA.
The Role of Mycorrhizae on the Growth of Banana Planlets of Cultivar Raja (Musa Paradisiaca C.V. Raja) from Bali at Post Acclimatization: Role of Mycorrhizae on the Growth of Banana Planlets Dwiyani, Rindang; Wirya, Gusti Ngurah Alit Susanta; Gunadi, I Gusti Alit; Darmawati, Ida Ayu Putri; Yuswanti, Hestin; Susrusa, Ketut Budi; AStiningsih, Ana Agung Made
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.11

Abstract

The effect of inoculation of a combination of the arbuscular mycorrhizal fungus of Glomus and Acaulospora on micropropagated banana plantlets cultivar Raja from Bali during the acclimatization phase was investigated. The experiment was conducted at the Experimental Station of the Faculty of Agriculture, Udayana University, Denpasar, Bali, Indonesia, over the period of March to June 2022. The objective of this research was to evaluate the impact of various doses of mycorrhizae on the growth of tissue culture banana plantlets that had undergone acclimatization for a period of six weeks. A total of five different doses of mycorrhiza were applied to the acclimatized plantlets, namely M0=without mycorrhiza and M5=5g mycorrhiza.kg-1, M10=10 g mycorrhiza.kg-1, M15=15 g mycorrhiza.kg-1, M20=20 g mycorrhiza.kg-1. A significant effect was discovered at M15; however, compared to M20, M20 gave better results for root length and fresh weight of the above-ground part, indicating the best dose was 20 g mycorrhiza kg-1. This study concluded that mycorrhiza has a very significant role in supporting the growth of micro propagated banana plantlets at post-acclimatization. Variables observed, such as the average number of leaves, stem diameter, above-ground plant fresh and dry weight, and root fresh and dry weight, increased with the application of AMF (Glomus and Acaulospora).
Morphological Characterization and Phytochemical Analysis of Moringa Plant (Moringa oleifera L.) Different Altitudes in Bali Danga, Jeni Rambu Yaku; Rai, I Nyoman; Dwiyani, Rindang
Agro Bali : Agricultural Journal Vol 8, No 2 (2025)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v8i2.1919

Abstract

The Moringa plant (Moringa oleifera L.) is a plant that has many benefits and has long been used by various groups throughout the world. Almost all parts of this magical plant can be used for multiple purposes. Moringa plants spread and adapt to different environments, increasing phenotypic and phytochemical diversity. The research was conducted at three altitudes in Bali, lowlands, medium, and highlands from December 2023–May 2024. The study was conducted to determine differences in morphological characters and phytochemical content of Moringa plants. This research used UPGMA analysis to determine the relationship based on morphological characters, flavonoids, tannins, and vitamin E using a UV-Vis Spectrophotometer, while the antioxidant activity test was carried out using the DPPH method. The results of the identification of morphological characters showed differences in qualitative characters, which were seen in leaf color and flower color, while quantitative characters were seen in leaf length, leaf thickness, pod length, and number of seeds. Nine Moringa accessions showed differences in phytochemical content. The highest flavonoid and vitamin E content in the lowlands are (599.961 mg QE/100 g) and (599.961 mg QE/100 g), tannins in the medium plains (368.140 mg TAE/100 g), and the highest IC50 in the highlands (54.94 ppm).
Co-Authors ADINDA RIZKI NURANA AJENG IDVATUL FITROH ANAK AGUNG MADE ASTININGSIH Ari Indrianto Ari Indrianto Ari Indrianto AStiningsih, Ana Agung Made AYU INDAH WIDAYANTI Azis Purwantoro Azis Purwantoro Azis Purwantoro Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto BARAELA EZRA WIJAYA Cokorda Gede Alit Semarajaya Danga, Jeni Rambu Yaku DINI WIRDASARI EKA CHRISTY WAKANNO Endang Semiarti ENDANG SEMIARTI Endang Semiarti ENIEK KRISWIYANTI GEDE WIJANA Gusti Ngurah Alit Susanta Wirya H. Yuswanti H. Yuswanti HESTIN - YUSWATI HESTIN YUSWANTI I GEDE PUTU WIRAWAN I GUSTI AGUNG INDRA MERTAWAN I Gusti Alit Gunadi I Kadek Wira Pradana I KETUT ARSA WIJAYA I Ketut Suada I MADE CHRISTIAN ADHI SAPUTRA I Made Sudana I Made Sudana I Made Sudana I Made Sukewijaya I NYOMAN GEDE ASTAWA I NYOMAN RAI I Putu Wahyu Sanjaya I WAYAN RUMADA I.A. MAYUN I.G.A. Gunadi I.N.G. Astawa IDA AYU ASTARINI Ida Ayu Putri Darmawanti Ida Ayu Putri Darmawati IDA AYU PUTRI DARMAWATI Ida Ayu Sri Dewi Adriani Ida Bagus Komang Mahardika Indrianto, A - Ixora Sartika Mercuriani K.A. Yuliadhi K.B. Susrusa KADEK PEBRIYANI KETUT BUDI SUSRUSA KETUT SUADA Ketut Suada LUH PUTU SUGIARI M. Sudiana Mahendra MADE SRITAMIN Manullang, Prila Kartika Muhammad Rivai N.N.A. Mayadewi N.N.A. Mayadewi NI LUH MADE PRADNYAWATHI NI LUH MARTINI NI NYOMAN ARI MAYADEWI NI NYOMAN SURYANTINI Ni Putu Ayu Erninda Oktaviani Suputri Purba, Reza Stefany Purwantoro, A - Putu Suwardike Retno Kawuri Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sanjaya, I Putu Wahyu Semiarti, E. - SONDANG RAJAGUKGUK TRISNA AGUNG PHABIOLA UTAMI - - Y. Fitriani YUDHANI WIDHYA HARTIWI Yuyun Fitriani