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The Role of Mycorrhizae on the Growth of Banana Planlets of Cultivar Raja (Musa Paradisiaca C.V. Raja) from Bali at Post Acclimatization: Role of Mycorrhizae on the Growth of Banana Planlets Dwiyani, Rindang; Wirya, Gusti Ngurah Alit Susanta; Gunadi, I Gusti Alit; Darmawati, Ida Ayu Putri; Yuswanti, Hestin; Susrusa, Ketut Budi; AStiningsih, Ana Agung Made
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.11

Abstract

The effect of inoculation of a combination of the arbuscular mycorrhizal fungus of Glomus and Acaulospora on micropropagated banana plantlets cultivar Raja from Bali during the acclimatization phase was investigated. The experiment was conducted at the Experimental Station of the Faculty of Agriculture, Udayana University, Denpasar, Bali, Indonesia, over the period of March to June 2022. The objective of this research was to evaluate the impact of various doses of mycorrhizae on the growth of tissue culture banana plantlets that had undergone acclimatization for a period of six weeks. A total of five different doses of mycorrhiza were applied to the acclimatized plantlets, namely M0=without mycorrhiza and M5=5g mycorrhiza.kg-1, M10=10 g mycorrhiza.kg-1, M15=15 g mycorrhiza.kg-1, M20=20 g mycorrhiza.kg-1. A significant effect was discovered at M15; however, compared to M20, M20 gave better results for root length and fresh weight of the above-ground part, indicating the best dose was 20 g mycorrhiza kg-1. This study concluded that mycorrhiza has a very significant role in supporting the growth of micro propagated banana plantlets at post-acclimatization. Variables observed, such as the average number of leaves, stem diameter, above-ground plant fresh and dry weight, and root fresh and dry weight, increased with the application of AMF (Glomus and Acaulospora).
Pengaruh Pemberian GA3 dan Ekstrak Paku Sayur (Diplazium esculentum) terhadap Kualitas Hasil Buah Anggur (Vitis vinifera L.) Varietas Kediri Kuning Purba, Reza Stefany; Astawa, I Nyoman Gede; Dwiyani, Rindang
Agrotrop : Journal on Agriculture Science Vol 14 No 2 (2024)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2024.v14.i02.p14

Abstract

Effect of GA3 and Vegetable Spike Extract (Diplazium esculentum) on the Yield Quality of Grape (Vitis vinifera L.) Kediri Kuning Variety. This study aims to determine the effect of vegetable fern extract solution on the quality of grapes of Kediri Kuning variety and the concentration that can improve the quality of grapes. This research uses RAK (Randomized Group Design) with the treatment of vegetable fern extract consisting of 5 levels with 5 replicates, namely P0 = Control, PG = GA3 500 ppm, PS25 = 25%, PS50 = 50%, PS75 = 75%. The results showed that the treatment of vegetable fern extract solution gave an influence on the variables of titratable acid content, fruit bunch length, number of seeds per fruit, vitamin c content, antioxidant properties content, and seed weight per fruit, tannin content. Dipping the vegetable fern extract solution in the stadia before flower blossom can improve the quality of Kediri Kuning grapes, seen in titratable acid content, fruit bunch length, number of seeds per fruit, weight of seeds per fruit, vitamin C content, antioxidant properties, and tannin content. The concentration of 50% vegetable spike extract is able to provide the best results and quality to improve the quality of Kediri Kuning grapes.
Intraspecific SSR Marker Screening for Detection of Dendrobium crumenatum Mutants Generated from In Vitro Gamma Irradiation Dwiyani, Rindang; Sanjaya, I Putu Wahyu; Yuswanti, Hestin; Darmawati, Ida Ayu Putri; Suada, I Ketut; Manullang, Prila Kartika; Fitriani, Yuyun
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.89896

Abstract

Determination of D. crumenatum mutant obtained from in vitro mutation breeding needs a long time due to its long-life cycle. SSR molecular markers can be used for early mutant detection. Specific SSR markers developed in D. crumenatum are not yet available. Alternative published SSR markers were developed from D. catenatum. The aims of this study are to screen the most informative SSR markers generated from D. catenatum tested in irradiated D. crumenatum population and to determine the gamma irradiation dose resulting the most mutants. Ten SSR markers were randomly selected and tested in 25 individuals of D. crumenatum plantlets irradiated with several doses (0, 5, 10, 15, and 20 Gy; 5 plantlets each dose). The result showed 7 of 10 primers were polymorphic and the other three were  monomorphic. All seven polymorphic primers can be used to identified intraspecific variation in the D. crumenatum mutant population. Markers dnsr28 and dnsr98 were the most informative, with the highest polymorphic information content (PIC) value of 0.5. Irradiation D. crumenatum protocorms using 10-15 gray doses were detected as the highest mutant percentage obtained up to 100% in the sample tested. This resulting marker information can be used to screen wider mutant population to decrease the non-mutant individuals in the population for maintenance and cost efficiency. The 10-15 Gy can be used as reference doses for gamma irradiation in 3 months old D. crumenatum protocorm materials. 
Meristem Culture of Strawberry Plants (Fragaria x Ananassa Duch.) Sachinoka Variety on Various Types of Basic Media In Vitro Fitriani, Yuyun; Yuswanti, Hestin; Dwiyani, Rindang; Darmawati, Ida Ayu Putri
International Journal of Biosciences and Biotechnology Vol 11 No 1 (2023): International Journal of Biosciences and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2023.v11.i01.p08

Abstract

The large number of in vitro media circulating in the market is a challenge to find the most effective media for sachinoka strawberry meristem culture, because the need for in vitro media depends on genetic intelligence. The purpose of this study was to find the best media from the various tested media to reproduce the Sachinoka variety strawberry seedlings in a relatively short time using the meristem culture method. This study used a completely randomized design (CRD) with 6 media treatments and repeated 7 times, namely: M1 (WPM without BA), M2 (WPM + 4 ppm BA), M3 (NP without BA), M3 (NP + 4 ppm BA), M5 (BMM without BA), M6 (BMM + 4 ppm BA). The variables observed were the time of emergence of shoots, the number of shoots and the number of leaves. The results of this study indicate that there is no significant difference for the emergence of shoots variable. Treatment M1 produced the highest number of shoots (12.29) but not significantly different from M5 (10.14), both were significantly different from both treatments. The highest number of leaves is in M1 (27.14). It can be concluded that the best medium for the growth of sachinoka strawberry meristem culture is WPM media with the addition of 4 ppm BA, while the second-best medium is BMM media with the addition of 4 ppm BA.
The Role of Mycorrhizae on the Growth of Banana Planlets of Cultivar Raja (Musa Paradisiaca C.V. Raja) from Bali at Post Acclimatization: Role of Mycorrhizae on the Growth of Banana Planlets Dwiyani, Rindang; Wirya, Gusti Ngurah Alit Susanta; Gunadi, I Gusti Alit; Darmawati, Ida Ayu Putri; Yuswanti, Hestin; Susrusa, Ketut Budi; AStiningsih, Ana Agung Made
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.11

Abstract

The effect of inoculation of a combination of the arbuscular mycorrhizal fungus of Glomus and Acaulospora on micropropagated banana plantlets cultivar Raja from Bali during the acclimatization phase was investigated. The experiment was conducted at the Experimental Station of the Faculty of Agriculture, Udayana University, Denpasar, Bali, Indonesia, over the period of March to June 2022. The objective of this research was to evaluate the impact of various doses of mycorrhizae on the growth of tissue culture banana plantlets that had undergone acclimatization for a period of six weeks. A total of five different doses of mycorrhiza were applied to the acclimatized plantlets, namely M0=without mycorrhiza and M5=5g mycorrhiza.kg-1, M10=10 g mycorrhiza.kg-1, M15=15 g mycorrhiza.kg-1, M20=20 g mycorrhiza.kg-1. A significant effect was discovered at M15; however, compared to M20, M20 gave better results for root length and fresh weight of the above-ground part, indicating the best dose was 20 g mycorrhiza kg-1. This study concluded that mycorrhiza has a very significant role in supporting the growth of micro propagated banana plantlets at post-acclimatization. Variables observed, such as the average number of leaves, stem diameter, above-ground plant fresh and dry weight, and root fresh and dry weight, increased with the application of AMF (Glomus and Acaulospora).
Morphological Characterization and Phytochemical Analysis of Moringa Plant (Moringa oleifera L.) Different Altitudes in Bali Danga, Jeni Rambu Yaku; Rai, I Nyoman; Dwiyani, Rindang
Agro Bali : Agricultural Journal Vol 8, No 2 (2025)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v8i2.1919

Abstract

The Moringa plant (Moringa oleifera L.) is a plant that has many benefits and has long been used by various groups throughout the world. Almost all parts of this magical plant can be used for multiple purposes. Moringa plants spread and adapt to different environments, increasing phenotypic and phytochemical diversity. The research was conducted at three altitudes in Bali, lowlands, medium, and highlands from December 2023–May 2024. The study was conducted to determine differences in morphological characters and phytochemical content of Moringa plants. This research used UPGMA analysis to determine the relationship based on morphological characters, flavonoids, tannins, and vitamin E using a UV-Vis Spectrophotometer, while the antioxidant activity test was carried out using the DPPH method. The results of the identification of morphological characters showed differences in qualitative characters, which were seen in leaf color and flower color, while quantitative characters were seen in leaf length, leaf thickness, pod length, and number of seeds. Nine Moringa accessions showed differences in phytochemical content. The highest flavonoid and vitamin E content in the lowlands are (599.961 mg QE/100 g) and (599.961 mg QE/100 g), tannins in the medium plains (368.140 mg TAE/100 g), and the highest IC50 in the highlands (54.94 ppm).
MORPHOLOGICAL CHARACTERIZATION AND PHYTOCHEMICAL ANALYSIS OF RATNA FLOWER PLANT (Gomphrena globosa) PINK PHENOTYPE IN SIBANGGEDE AND PADANG GALAK AREAS Redana, I Made Basma; Dwiyani, Rindang; Darmawati, Ida Ayu Putri
Jurnal Agrotek Tropika Vol. 13 No. 3 (2025): JURNAL AGROTEK TROPIKA VOL 13, AGUSTUS 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i3.9681

Abstract

The Ratna flower (Gomphrena globosa L.), commonly known as globe amaranth, is widely used as an ornamental plant, natural tea ingredient, food coloring, and traditional medicine due to its various health benefits. In Bali, this flower also holds important cultural value as an offering in ceremonies and sacred dances. Despite its diverse uses, studies on the morphological and phytochemical diversity of G. globosa accessions across different growing regions remain limited. This study aimed to characterize the morphological traits and analyze the phytochemical composition of pink-flowered Ratna accessions collected from two regions, Sibanggede and Padang Galak. The research was conducted from October to December 2023. Sampling areas were determined using an exploratory method with purposive sampling, and plant samples were collected using a simple random sampling approach. The findings revealed distinct morphological variations in leaf and stem surfaces between accessions from the two locations. Phytochemical analysis showed qualitative and quantitative differences in chemical composition. The Sibanggede accession contained major compounds such as phytol, ethyl ester, and hexadecanoic acid—known for their antioxidant, anticancer, and antibacterial properties. In contrast, the Padang Galak accession was dominated by compounds including 2(4H)-Benzofuranone and thiourea, which exhibit antiarthritic and anticancer activities. These morphological and phytochemical differences are likely influenced by environmental factors affecting plant development and secondary metabolite synthesis. Overall, this study provides baseline information for the cultivation and utilization of Ratna flower in pharmaceutical, cosmetic, and ethnobotanical applications.
Optimization of Growing Media to Support Microgreens Growth and Nutritional Profile Sukewijaya, I Made; Dwiyani, Rindang; Bimantara, Putu Oki
Agro Bali : Agricultural Journal Vol 8, No 1 (2025)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v8i1.2249

Abstract

Microgreens are young seedlings of edible vegetables, herbs, and flowers. Growing media plays a vital role in plant growth and the biosynthesis of multiple metabolites that improve the nutritional profile of microgreen. This study evaluated the effect of growing media from a combination of soil, husk charcoal, and perlite with a specific ratio of 1:1:1 (TAP 111), 2:1:1 (TAP 211), 1:2:1 (TAP 121), and 1:1:2 (TAP 112) on the nutrition profile and fresh weight of several microgreen plants, including water spinach, red spinach, green mustard, red lettuce, green spinach, and bok choy. The nutrient contents of nitrogen, phosphorus, and potassium in the growing media were quantified in this study. The data analysis was performed using Duncan’s multiple range test to assess the quantity of vitamin A, vitamin C, antioxidant capacity, and fresh weight at a 5% confidence level. The results showed that TAP121 media exhibited moderate levels of nitrogen (0.23%), very high phosphorus (238.68 ppm), and high potassium (324.69 ppm. The highest vitamin A was found in red spinach in TAP111 (27.77 mg 100 g-1) and TAP112 (22.72 mg 100 g-1) media. The highest vitamin C was found in green mustard in TAP111 media (66.44 mg 100 g-1) and in bok choy in TAP112 media (61.25 mg 100 g-1). The highest antioxidant capacity was found in Bok choy in TAP121 media (386.4 mg AAEAC 1000 g-1) and the highest fresh weight was found in water spinach in  TAP121 media (4.03 g). In conclusion, TAP121 media can be recommended to support the balanced growth and nutritional quality of microgreen plants, especially bok choy, and water spinach. This study provides insights into how specific combinations of growing media can enhance the growth and nutritional content of microgreens. Future studies could focus on optimizing nutrient levels and environmental conditions to maximize the antioxidant properties and other bioactive compounds in microgreens.
Pengaruh Penambahan Trichoderma Spp. pada Media Tanam Terhadap Pertumbuhan Bibit Pisang (Musa acuminata) Cavendish Hasil Kultur Jaringan Pradana, I Kadek Wira; Dwiyani, Rindang; Wirya, Gusti Ngurah Alit Susanta
Agro Bali : Agricultural Journal Vol 7, No 1 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i1.1568

Abstract

Trichoderma merupakan salah satu mikroorganisme spesifik spesies, jadi masing-masing spesies Trichoderma akan memberikan pengaruh yang berbeda terhadap spesies tanaman yang berbeda. Penelitian ini dilakukan dengan tujuan untuk mengetahui Pengaruh Penambahan Trichoderma spp. Pada Media Tanam Terhadap Pertumbuhan Bibit Pisang (Musa acuminata) Cavendish Hasil Kultur Jaringan. Penelitian ini menggunakan rancangan percobaan satu faktor dengan rancangan lingkungan RAL. Trichoderma ditambahkan dengan perbedaan spesies Trichoderma yaitu T0 = Kontrol, Ta = T. asperellum., Th= T. harzianum, Tk = T. koningii, Tv = T. viride. Tahapan penelitian aklimatisasi plantlet tahap satu (3 minggu), inkubasi Trichoderma pada kompos (2 minggu), aklimatisasi tahap dua (6 minggu). Data yang diperoleh dari pengukuran, penghitungan dan pengamatan variabel dianalisis secara statistik dengan menggunakan analisis of variance (ANOVA). Hasil analisis ANOVA menunjukkan bahwa aplikasi Trichoderma spp. pada tanaman pisang (Musa Acuminata) cavendish hasil kultur in vitro berpengaruh tidak nyata P ≥ 0,05 terhadap semua variabel yang diamati. Namun ada kecenderungan pertambahan jumlah helai daun Th lebih banyak 1,75 helai dibanding T0, panjang akar Th lebih panjang 6,525 cm dibanding T0.
Improvement of Genetic Transformation Efficiency in Vanda tricolor Orchid Using Acetosyringone Dwiyani, Rindang; Purwantoro, Azis; Indrianto, Ari; Semiarti, Endang
Annales Bogorienses Vol. 14 No. 2 (2010): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Vanda tricolor Lindl. var. suavis is an Indonesian wild orchid which is now extremely rare in nature due to its habitat destruction. Development of an appropriate method for improving Vanda orchid through genetic modification could be valuable for horticulture and, indirectly, also for conservation. In this research, a method of Agrobacterium-mediated transformation of two V. tricolor obtained from Salak Mount, West Java and Merapi Mount, Yogyakarta in Indonesia protocorms was improved using acetosyringone (AS). Concentrations of 0 and 25 ppm AS were used in transformation of pG35S binary vector containing kanamycin resistance geneinto V. tricolor protocorms. The result showed that 25 ppm AS was required on inoculation with Agrobacterium solution, without AS on cocultivation. Five weeks after treatment on the 300 ppm kanamicyn containing medium, green protocorms were obtained, that was 11.01% for V. tricolor from Salak Mount with pre-culture treatment prior to inoculation, 9.39% for V. tricolor from Merapi Mount with pre-culture treatment prior to inoculation, and 1.37% for V. tricolor from Merapi Mount without pre-culture treatment prior to inoculation. The best condition to set high efficiency of transformation is pre-culture protocorms prior inoculation, soaking protocorm on 25 ppm AS for 30 minutes, then co-cultivate its on AS-free callus induction medium.
Co-Authors ADINDA RIZKI NURANA AJENG IDVATUL FITROH ANAK AGUNG MADE ASTININGSIH Ari Indrianto Ari Indrianto Ari Indrianto AStiningsih, Ana Agung Made AYU INDAH WIDAYANTI Azis Purwantoro Azis Purwantoro Azis Purwantoro Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto BARAELA EZRA WIJAYA Bimantara, Putu Oki Cokorda Gede Alit Semarajaya Danga, Jeni Rambu Yaku DINI WIRDASARI EKA CHRISTY WAKANNO Endang Semiarti ENDANG SEMIARTI Endang Semiarti ENIEK KRISWIYANTI GEDE WIJANA Gusti Ngurah Alit Susanta Wirya H. Yuswanti H. Yuswanti HESTIN - YUSWATI HESTIN YUSWANTI I GEDE PUTU WIRAWAN I GUSTI AGUNG INDRA MERTAWAN I Gusti Alit Gunadi I KETUT ARSA WIJAYA I Ketut Suada I MADE CHRISTIAN ADHI SAPUTRA I Made Sudana I Made Sudana I Made Sudana I Made Sukewijaya I NYOMAN GEDE ASTAWA I NYOMAN RAI I Putu Wahyu Sanjaya I WAYAN RUMADA I.A. MAYUN I.G.A. Gunadi I.N.G. Astawa IDA AYU ASTARINI Ida Ayu Putri Darmawanti Ida Ayu Putri Darmawati IDA AYU PUTRI DARMAWATI Ida Ayu Sri Dewi Adriani Ida Bagus Komang Mahardika Indrianto, A - Ixora Sartika Mercuriani K.A. Yuliadhi K.B. Susrusa KADEK PEBRIYANI KETUT BUDI SUSRUSA KETUT SUADA Ketut Suada LUH PUTU SUGIARI M. Sudiana Mahendra MADE SRITAMIN Manullang, Prila Kartika Muhammad Rivai N.N.A. Mayadewi N.N.A. Mayadewi NI LUH MADE PRADNYAWATHI NI LUH MARTINI NI NYOMAN ARI MAYADEWI NI NYOMAN SURYANTINI Ni Putu Ayu Erninda Oktaviani Suputri Pradana, I Kadek Wira Purba, Reza Stefany Purwantoro, A - Putu Suwardike Redana, I Made Basma Retno Kawuri Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sanjaya, I Putu Wahyu Semiarti, E. - SONDANG RAJAGUKGUK TRISNA AGUNG PHABIOLA UTAMI - - Y. Fitriani YUDHANI WIDHYA HARTIWI Yuyun Fitriani