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All Journal JBIO: jurnal biosains (the journal of biosciences) Indonesian Journal of Tropical and Infectious Disease Biota Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology BIOTROPIC The Journal of Tropical Biology Indonesia Journal of Halal Indonesian Journal of Halal Research Journal of halal product and research (JHPR) Jurnal Riset Biologi dan Aplikasinya Sains dan Matematika Genbinesia Journal of Biology
Yuanita Rachmawati
UIN Sunan Ampel Surabaya
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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End Point Polymerase Chain Reaction for Porcine Detection on Food Product of UIN Sunan Ampel Surabaya Canteen Rokhim, Saiku; Tyautari, Inggrit; Firmansyah, M. Aliffiyan; Rachmawati, Yuanita
Indonesian Journal of Halal Research Vol. 3 No. 1 (2021): February
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v3i1.11149

Abstract

Halal food means food that permitted under Islamic law and fulfills about requirements. The absence of information about halal food contained in UIN Sunan Ampel Surabaya (UINSA) campus area causes related research to be carried out. This study aims to determine the porcine DNA contamination on food around UINSA area using molecular technology. Twenty two samples used were foods that contain meat and may contain pork obtained from canteens around UINSA area, analyzed using Polymerase Chain Reaction (PCR) method. The analysis was started with DNA isolation of 22 food samples, electrophoresis, PCR, then visualization gel electrophoresis. Primer gene coding for cytochrome b (cyt b) which produces 149 bp of DNA fragments. The results showed that no porcine contamination in 22 food samples, while the positive control showed a band of 149 bp. End point PCR method potentially to detect porcine DNA contaminants in food products around UINSA. Therefore the food is halal and safe for consumption.
Co-Authors Adzakiyyi, Miftahul Lathif AGUSTINA, EVA Aini, Humayra Qurrata Aini, Nur Sofiatul Ameliora C E Arrohmah, Robiatush Sholichah Ayudya Fitri Arifa Azzhahara, Nabillah Ayu Bahri, Saiful Bening Larasati BUDI SETIADI DARYONO Choiriyah, S.Si, Chumaidatul DAMAYANTI, MAMIK Dian Astriana Eko Prasetya Eko Prasetya, Eko Esti Tyastirin Fauzana Putri Fauziyah Harahap Febri Yuda Kurniawan Firmansyah, M. Aliffiyan Galang Riswi Dyatama Ganies Riza Ariestya Ganies Riza Aristya Hary Prakasa Himawan Masyhuri Humayra Qurrata Aini Inggrit Tyautari Inggrit Tyautari Irul Hidayati Kautsar, Radinal KHOIRIYAH, ROMYUN ALVY Latifatoel Chilmi Lazuardi Lazuardi M. Aliffiyan Firmansyah Mabruroh, Arofatul Syabina Mahbubillah, M. Ainul Mashita Andiana, Mashita Miftahul Jannah Miftahul Lathif Adzakiyyi Misbakhul Munir Misbakhul Munir Moch. Irfan Hadi Nabila Ayu Nabila, Salsa Najwa Maulidina P Ninik Fadhillah Nirmala Fitria Firdhausi Oki Rahmatirta W Pradiptaadi, Brian Pramana Aprilio Puji Prastowo, Puji Putri, Siti Aisyah Bisri Radinal Kautsar Ramadhani, Aisyah Hadi Said . Iskandar Saiku Rokhim Setiyowati, Putri Ayu Ika Siti Latifa Siti Malihatus Sa'adah Sri Susila Andayani, Sri Susila Teguh Hari Sucipto, Teguh Hari Tri Susilowati Tri Susilowati Tri Susilowati Tyautari, Inggrit Yusuf, Yusnaeni