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UJI BIOAKTVITAS ANTIBAKTERI EKSTRAK MANGROVE TERHADAP BAKTERI PENYEBAB PENYAKIT ACUTE HEPATOPANCREATIC NECROSIS DISEASE Andi Hamdillah; andi Muhammad Akram; Harlina Harlina; Ilmiah Ilmiah
JURNAL AKUAKULTUR, TEKNOLOGI DAN MANAJEMEN PERIKANAN TANGKAP, ILMU KELAUTAN Vol 7 No 2 (2024): JOINT-FISH - Jurnal Akuakultur, Teknologi Dan Manajemen Perikanan Tangkap, Ilmu K
Publisher : Fakultas Perikanan dan Ilmu Kelautan Universitas Muslim Indonesia Makassar, Sulawesi Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/joint-fish.v7i2.562

Abstract

Acute Hepatopancreatic Necrosis Disease (AHPND) is one of the most detri-mental diseases for shrimp farmers, with a mortality rate reaching 100%. One approach to treating AHPND infections involves utilizing mangrove extracts. Several secondary metabolites found in mangroves, such as steroids, triterpenoids, flavonoids, alkaloids, and polyphenols, possess antibacterial properties. The objective of this study is to evalu-ate the antibacterial activity of various mangrove extracts against Vibrio parahaemolyticus strain AHPND. The research method, extraction uses the maceration method. The anti-bacterial activity test used the double layer diffusion method to isolate the bacteria V. parahaemolyticus strain AHPND.. Further tests were carried out to determine the Mini-mum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the mangrove extracts. Results: The study revealed that extracts from A. officinalis and R. apiculata inhibited the growth of V. parahaemolyticus, with inhibition zone diameters of 11 mm and 12 mm, respectively. Further testing showed that the MIC and MBC values of A. officinalis were 0.25 mg/disc, producing an inhibition zone diameter of 8 mm, while the MIC and MBC values of R. apiculata were also 0.25 mg/disc, with inhibition zone diameters of 8 mm for MIC and 7 mm for MBC. Avicennia officinalis and Rhizophora apiculata show potential to be developed as natural antibacterial agents in aquaculture.
EFFECT OF PROBIOTICS ADDED MOLASSES IN FEED ON GROWTH AND SURVIVAL OF MILKFISH (Chanos chanos) Wakka, Fahad Bin Muhammad Nur; Ilmiah, Ilmiah; Hamdillah, Andi
Jurnal Perikanan Unram Vol 15 No 3 (2025): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v15i3.1507

Abstract

Chanos chanos is classified as a type of cultured fish with great economic value because it plays an important role in meeting the nutritional needs of the community and contributes in improving the welfare of life. Giving probiotics is one way that can be used to overcome environmental pollution. Probiotics themselves are living microorganisms that function to maintain the stability of the digestive system in the intestine. Molasses acts as a source of nutrients for probiotic bacteria and is expected to support the increase in the effectiveness of its population as bioremediation agents can be more optimal. This study aims to evaluate the effect of the addition of probiotics combined with molasses in feed on the growth and survival rate of milkfish (Chanos chanos). This research was conducted from October 10 to December 7, 2024 at Tadang Palie, Kec. Cempa. Pinrang Regency, South Sulawesi with a duration of 60 days. This research was conducted using a completely randomized design (CRD), which involved four different treatments and each treatment was repeated three times. The results showed that the effect of probiotics added to molasses in feed had a significant effect on the growth of absolute weight, specific, absolute length of milkfish. For absolute weight growth, the highest value was in treatment B (7.42 grams), the highest value of specific weight was in treatment B (9.86), the highest value of absolute length was in treatment B (9.4 cm), while survival and FCR had no significant effect on milkfish. The highest survival rate was found in treatment A (87%), and the best FCR value in treatment B (3.08).
Effectiveness of Betel Leaf Extract (Piper betle L.) against bacterial infection (Vibrio paraharmolyticus) in Vaname Shrimp (Litopenaeus vannamei) Panduwinata, Vina; Hadijah, St; Harlina, Harlina; Hamdillah, Andi; Ilmian, Ilmian
Agrikan Jurnal Agribisnis Perikanan Vol. 18 No. 1 (2025): Agrikan: Jurnal Agribisnis Perikanan
Publisher : Fakultas Pertanian, Universitas Muhammadiyah Maluku Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52046/agrikan.v18i1.2527

Abstract

This research aims to identify the types of bioactive compounds in the methanol extract of betel leaves (Piper betle L) observing the Clear Zone. And knowing the inhibitory ability of methanol extract of betel leaves (Piper betle L). through determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against V. parahaemolyticus. This research used experimental methods and completely random sampling (RAL). By carrying out a GC-MS test to identify the bioactive compounds contained in betel leaves. Antibacterial activity test and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests to determine the ability of betel leaf extract to inhibit Vibrio parahaemolyticus bacteria. And a challenge test to determine the survival rate. The results of descriptive analysis and Anova statistical tests show that betel leaf extract contains 5 secondary metabolic compounds. The use of betel leaf extract 10 mg/ml, 15 mg/ml, 20 mg/ml shows strong inhibitory power with a minimum concentration of 3.12 mg/ml and 6.25 mg/ml after the Survival Rate challenge test was carried out for vaname shrimp treated with 85% betel leaf extract, while the administration of betel leaves was also able to reduce the clinical symptoms experienced by vaname shrimp with a concentration of 50 mg/ml. Conclusion: These results identify that betel leaf extract has secondary metabolic compounds that can be used as antibacterials in white shrimp cultivation activities.