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Application of the flower of Beauveria bassiana Through immersion Seeds and It's effect on Colonization and content of Chlorophil leaves of red chilli (Capsicum annuum L.) Magdalena , Saragih; Trizelia; Nurbailis; Yusniwati
Jurnal Pertanian Tropik Vol. 8 No. 2 (2021): JURNAL ONLINE PERTANIAN TROPIK
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (556.214 KB) | DOI: 10.32734/jopt.v8i2.6519

Abstract

Endophytic fungi in lately was getting a lot of attention in agriculture. Besides being able to increase plant resistance against plant pest organism, it has also the ability to stimulate germination and pant growth. One of endophytic fungi tested in its research was Beauveria bassiana from isolate Wheat, Coffeae, Cacao and entomopatogen fungus from Leptocorisa acuta insect. The aim of this research was to know the ability of Beauveria bassiana from some tested isolates and its effect on chlorophyll content of leaf chili followed Mack Kinner methode by doing seed treatment aplication. Colonization and chlorophyl content of chili leaf was done on 7 MPI. The research design used Random Block Design non factorial, data was analysis by 8 STAT. The result showed all of Beauveria bassiana isolates were able to colonize and established as endophytic in chili plant.Beauveria bassiana of wheat isolate was the best isolate giving spurring germination and seedling plant growth and not significant with B.bassiana isolate from Leptocorisa acuta insect. Aplication of B.bassiana isolates was able to increase the chlorophyll contentof chilileaf compared control. All of the tested B.bassiana fungus could increased chlorophyl-b content and chlorophyl totally of chili leaf, whereas chlorophyl-a content all of tested isolates was not significant. From its research showed there were corelation between colonization and leaf chlorophyll content, the higher percentage of endophytic fungus on leaf chili, it made higher the chlorophyl-b content and chlorophyl totally content of leaf chili.
Effect of several types of endophytic fungi isolates Beauveria bassiana in suppressing seed-borne pathogens Colletotrichumspp Dini Puspita , Yanty; Trizelia; Darnetty; Jumsu , Trisno
Jurnal Pertanian Tropik Vol. 8 No. 2 (2021): JURNAL ONLINE PERTANIAN TROPIK
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (674.793 KB) | DOI: 10.32734/jopt.v8i2.6520

Abstract

Anthracnose is one of the most important seed-borne diseases in chili plants because this disease can reduce the quantity and quality of chilies and cause substantial economic losses. The aim of this study was to obtain the best isolates from various types of endophytic fungi Beauveria bassiana in suppressing the growth of pathogens. chili seed borne disease caused by the fungus Colletotrichum spp. The design used in this study was a completely randomized design (CRD) consisting of 6 treatments. The treatments consisted of control and 5 types of B. bassiana isolates (PD114, TD312, WS, BbKo, KT2B23). Parameters observed in this study were chili seeds that were attacked by Colletotrichum spp., normal seed germination on paper-to-paper test, length of plumule and radicle. Results. The study showed that different types of B. bassiana isolates could reduce the attack rate of seed-borne pathogenic fungi Colletotrichum spp. KT2B23 isolate was the best isolate in suppressing chili seed-borne pathogens.
Cendawan entomopatogen sebagai penginduksi ketahanan tanaman: Sebuah tinjauan sistematis: Entomopathogenic fungi as plant resistance inducer: A systematic review Indri Yanil Vajri; Trizelia Trizelia; Retna Astuti Kuswardani; Magdalena Saragih
Jurnal Entomologi Indonesia Vol 21 No 1 (2024): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.21.1.75

Abstract

IInsect pathologists' attention to the potential of entomopathogenic fungi (EPF) as inducers of plant resistance has increased in recent years. Several types of EPF that have received attention as biocontrol agents for insect pest populations include Beauveria bassiana, Metarhizium anisopliae and Trichoderma sp. because they are able to infect and kill insects directly, has a wide host range, and has been used to control various types of insect pests. Apart from being able to infect and kill insect pests, EPF can also live endophytically in plant tissue and increase plant resistance to pest attacks. The presence of EPF as endophytes is able to induce plant resistance by stimulating an increase phytohormones production. In this article, we reviewed the role of fungi as entomopathogens, the characteristics of entomopathogenic fungi, and their role as inducers of plant resistance. The review was carried out by collecting information  from original articles and related reports which reviewed the results of research on the role of entomopathogenic fungi as plant resistance inducers by including the keywords entomopathogenic endophytic fungi, resistance induction, characterization, systemic resistance and herbivorous insects. This systematic review concludes that EPF as an inducer of plant resistance is a very promising control alternative in the development of plant protection management against insect pests.
Colonization of the entomopathogenic fungus Beauveria bassiana (Bals.) Vuill. on rice and its impact on nymph mortality and fecundity of brown planthopper (Nilaparvata lugens Stål): Kolonisasi cendawan entomopatogen Beauveria bassiana (Bals.) pada tanaman padi dan pengaruhnya terhadap mortalitas nimfa dan keperidian wereng batang coklat (Nilaparvata lugens Stål) Yolma Hendra; Trizelia Trizelia; My Syahrawati
Jurnal Entomologi Indonesia Vol 20 No 3 (2023): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.3.203

Abstract

Brown planthopper (BPH), Nilaparvata lugens Stål, is a significant pest widely recognised for its detrimental impact on rice production. Biological agents, such as the entomopathogenic fungus Beauveria bassiana (Bals.) Vuill., can effectively control this pest. B. bassiana, known for its endophytic abilities, colonises various plant tissues. This research aims to assess B. bassiana's colonosation capacity on rice plants and its impact on nymph mortality and BPH fecundity. Four isolates of B. bassiana (BbWS, Pb211, Td312, and BbJg) were used, each with conidial density of 108 conidia/ml. The application menthod involved soaking rice seeds in the fungus for 24 hours. The results showed that all B. bassiana isolates could successfully establish as endophytes within rice plants, colonising all tissue parts, with leaves exhibiting the highest fungal colonisation at 58%. Seed soaking with B. bassiana reduced eggs hatching by 23.88% and increased nymph mortality by 40%. The presence of B. bassiana in rice tissue also influenced BPH adults fecundity. Notably, the BbWS isolate demonstrated the most significant effectiveness in elevating nymph mortality and reducing BPH fecundity.
Eksplorasi Populasi dan Kepadatan Genus Nematoda Parasit pada Rizosfir Tanaman Kopi di Kota Solok, Sumatera Barat: Exploration of Population and Density of Parasitic Nematode Genus in Coffee Plant Rhizom in Solok City, West Sumatra Duma Putri Tama; Winarto; Trizelia
Jurnal Riset Perkebunan Vol. 5 No. 1 (2024): Jurnal Riset Perkebunan (JRP)
Publisher : Jurusan Budidaya Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrp.5.1.1-10.2024

Abstract

Coffee is a plantation crop that has high economic value among other plantation crops and plays an important role as a source of foreign exchange for the country. The decline and low productivity of coffee plants in coffee production centers in Solok City, West Sumatra is one of the causes of plant parasitic nematode attacks. This nematode attack causes disrupted plant growth and reduces the value of crop production both in quantity and quality. This study aims to determine the diversity and population density of parasitic nematodes in the rhizosphere of coffee plants in Solok City, West Sumatra. This research was conducted from March to July 2023 at the Laboratory of Biological Control, Department of Plant Protection, Faculty of Agriculture, Andalas University. Soil samples were taken in two central areas of coffee plantations in Solok City, West Sumatra. In the rhizosphere of coffee plants in Solok City, four genera of parasitic nematodes were found, namely: the genera Pratylenchus, Helicotylenchus, Meloidogyne, Rotylenchulus, Paratylenchus, and Tylenchorhynchus. The highest frequency of presence of nematodes, namely the genus Rotylenchulus, was 50.99 belonging to the medium category in Jorong Kayu Aro, and 44.80 in the rare category in Jorong Kayu Jao.
Ability of Beauveria bassiana to suppress Colletotrichum truncatum and increase the growth of chili plants (Capsicum annuum) Yanty, Dini Puspita; Trizelia, Trizelia; Darnetty, Darnetty; Trisno, Jumsu
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 8 No. 1 (2024): June 2024
Publisher : Department of Plant Protection, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.1.42-52.2024

Abstract

Anthracnose is a disease caused by Colletotrichum sp., which can reduce the quality and yield of red chili by 45-60%. This study aimed to determine the ability of different Beauveria bassiana isolates to control anthracnose in chili. The research employed a Randomized Block Design (RBD) consisting of 6 treatments and 5 replications. The treatments included a control and 5 different B. bassiana isolates (PD114, TD312, WS, BbKo, KT2B21). The observed variables included incubation period, disease incidence and severity, plant height, number of leaves, flowering time, and chili fruit weight. The results showed that the application of different B. bassiana isolates have effectively inhibited the development of Colletotrichum truncatum, which caused anthracnose in chili. B. bassiana has demonstrated the ability to prolong the incubation period of C. truncatum (5.40 days) and reduce the percentage of disease incidence (16%). Furthermore, the application of B. bassiana increased the height of chili plants (40.40 cm) and the number of leaves (32 leaves), accelerated the flowering time (42.80 days), and boosted chili production (178.25 g). The WS and TD312 isolates revealed the most significant results. Although the severity of the disease was not impacted, the 2 isolates provided notable agronomic benefits to the chili plants.
PEMBERDAYAAN KELOMPOK TANI MELALUI PELATIHAN PENGENDALIAN HAMA DAN PENYAKIT TANAMAN KAKAO DI KANAGARIAN CAMPAGO KABUPATEN PADANG PARIAMAN Ujang Khairul; Trizelia Trizelia; Reflin Reflin
BULETIN ILMIAH NAGARI MEMBANGUN Vol. 1 No. 4 (2018)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bina.v1i4.41

Abstract

Training on methods of pests and diseases management in cocoa plants has been conducted in the Nagari Campago, Kecamatan V Koto Kampung Padang Pariaman. The aims of this activity was to empower farmer groups through increasing the knowledge of cocoa farmers about cultivation and pests and diseases management attacked of cocoa plant, this activity consists of socialization, counseling and field practice. Evaluations carried out before and after training related to farmers' knowledge about cultivation and pests and diseases in cocoa. The results of the activity show that: (a) the level of pests and diseases in cocoa plantations in this area is quite high (55% dan 65%), (b) farmers have not good cultivated on cocoa, (c) farmers do not understand abaut pests and diseases that attack their cocoa and do not understand the methods for controlling pests and diseases. This activity has increased farmers' knowledge about the cultivation and management of pests and diseases in cocoa plants.
Induction of Resistance to Larvae Crocidolomia pavonana F. (Lepidoptera: Crambidae) using Rhizobacteria to the Cabbage Indri Yanil Vajri; Trizelia; Haliatur Rahma
Andalasian International Journal of Entomology Vol. 2 No. 1 (2024)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aijent.2.1.15-23.2024

Abstract

Crocidolomia pavonana is a significant pest on cabbage that reduces the quality and quantity of cabbage. Utilizing microorganisms such as rhizobacteria is an alternative environmentally friendly control that can potentially suppress the development of this pest. The study aimed to obtain rhizobacteria isolates capable of colonizing cabbage tissue and inducing plant resistance to C. pavonana larvae. The research was conducted at the Biological Control Laboratory and Greenhouse, Faculty of Agriculture, Universitas Andalas, Padang. The study used a Completely Randomized Design (CRD) with ten treatments and five replications. The treatment consisted of rhizobacteria isolates, including Bacillus thuringiensis, Bacillus subtilis, Serratia marcescens, Stenotrophomonas maltophilia, as well as a negative control (aquadest sterile) and a positive control (Cypermethrin insecticide). The test was carried out by soaking the seeds in a suspension containing rhizobacteria with a population density of 10⁸ cells/ml. The variables observed were larval mortality, pupa and imago formation percentage, and increased salicylic acid production. The data were analyzed using variance and continued with the LSD further test at the 5% level. The results showed that all rhizobacteria isolates colonized into cabbage plant tissue could kill C. pavonana larvae and inhibit these insects' biological development. B. thuringiensis KJKB7.3 showed better results with the highest mortality value (62.67%). Soaking cabbage seeds with rhizobacteria can increase the content of salicylic acid. Based on this research, the rhizobacteria used in the research have the potential to be developed as biological agents to control C. pavonana.
Endophytic Fungus Trichoderma asperellum's Virulence on Spodoptera frugiperda J. E. Smith (Lepidoptera, Noctuidae) Eggs Alfasiri, Ayubi; Trizelia, Trizelia; Nurbailis, Nurbailis
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 4 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.4.741

Abstract

Spodoptera frugiperda J.E. Smith, sometimes known as armyworm, is a serious pest in maize crops. This pest affects maize plants' leaves, resulting in yield losses of up to 79.9%. Entomopathogenic fungi is one alternate method for controlling the pest. Various entomopathogenic fungus can be found in nature, one of which thrives endophytically on plants, such as Trichoderma asperellum. The purpose of this study was to obtain and evaluate T. asperellum's pathogenicity in suppressing S. frugiperda eggs. This investigation used five T. asperellum isolates (A116, PC21, S2D11, SD34, and AB2B3) obtained from diverse plant tissues. S. frugiperda larvae were treated with 2 mL of a conidia suspension containing 108 conidia/mL and sterile distilled water as a control. The observation variables comprised S. frugiperda egg mortality, first-instar larvae mortality, pupae formation percentage, and imago formation percentage. The findings indicated that the endophytic fungus T. asperellum can infect S. frugiperda eggs. The mortality rate for S. frugiperda eggs ranged from 43.27 to 78.34%. The mortality rate of first instar larvae ranged between 36.94% and 60.22%. The application of T. asperellum to S. frugiperda decreased pupae and imago production by 39.78% and 37.87%, respectively. T. asperellum SD324 is the most effective isolate for infecting S. frugiperda eggs. Keywords: endophytes fungus, entomopathogenic fungus, larvae, maize, Spodoptera frugiperda
Isolation, Morphological Characterization, Biochemistry, and Antagonistic Activity of Endophytic Bacteria from Rice Against Xanthomonas oryzae pv. Oryzae Sihotang, Saipul; Butar-Butar, Alfons Dwi Putra; Nasution, Lita; Rahma, Haliatur; Trizelia, Trizelia; Saragih, Magdalena; Kuswardani, Retna Astuti; Listiana, Ika; Napitupulu, Beatrix Sofranes
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.825-829

Abstract

Endophytic bacteria have the potential as environmentally friendly biocontrol agents in controlling bacterial leaf blight (BLB) in rice caused by Xanthomonas oryzae pv. oryzae (Xoo). This study aims to isolate, characterize morphology and biochemical tests, and test the antagonistic activity of endophytic bacteria from rice against Xoo. Isolation was carried out from healthy rice root tissue using a surface sterilization method. Morphological characterization included colony shape, color, edge, elevation, and Gram properties, while biochemical characterization included catalase, oxidase, motility, starch hydrolysis, and sugar fermentation tests. The antagonistic test used a dual culture assay method on NA (nutrient agar) media. The isolation results obtained six endophytic bacterial isolates (BETP 01–06) identified as the genus Pseudomonas and Bacillus with varying colony morphological characters. Antimicrobial activity test showed two potential isolates, BETP 03 (Pseudomonas) and BETP 05 (Bacillus), with an average inhibitory diameter of 16.3 mm and 16.5 mm against Xoo, respectively. Biochemical characterization showed that BETP 03 was bacillus, Gram-negative, citrate-negative, motile, gelatin hydrolysis positive, starch hydrolysis positive, and catalase positive; while BETP 05 was bacillus, Gram-positive, citrate-positive, motile, gelatin hydrolysis negative, starch hydrolysis positive, and catalase positive. These results indicate that both isolates have high potential as biological biocontrol agents in controlling bacterial leaf blight disease in rice.