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Virulence of Five Isolates of The Entomopathogenic Fungus, Metarhizium anisopliae, Against Brown Planthopper (Nilaparvata lugens) Trizelia Trizelia; Haliatur Rahma; My Syahrawati
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 7 No. 2 (2023): December 2023
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.7.2.127-133.2023

Abstract

The brown planthopper (Nilaparvata lugens) is one of the main pests of rice plants. This pest attack can cause crop failure or puso. Biological control of this pest can be carried out using the entomopathogenic fungus, Metarhizium anisopliae. The ability of this fungus to control pests is influenced by the source of the isolate. The purpose of this study was to obtain isolates of M. anisopliae, which is virulent to N. lugens. This study used an experimental method with a completely randomized design (CRD) in six treatments and five replications. There were five isolates of M. anisopliae used and one control. M. anisopliae was applied to nymphs and adults of N. lugens with a concentration of 108 conidia/mL. The research revealed that all M. anisopliae isolates tested were virulent against N. lugens nymphs and adults. The most virulent isolate for the two stages of BPH was Met3B, which was collected from the leek rhizosphere. The isolate caused an adult mortality rate of 52%, nymph mortality of 60% in 10 days after application, and adults formed after application was only 37.33%.
Exploration and Characterization of Rhizobacteria from Rhizosphere of Rice Plants to Control Fusarium Fujikuroi In Vitro Zola Puti Rahma; Haliatur Rahma; Eri Sulyanti
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 8 No. 2 (2024): December 2024
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.2.123-137.2024

Abstract

Rhizobacteria is bacteria that found in the root zone of plants. Rhizobacteria can act as biocontrol agents against Fusarium fujikuroi, the fungus that causes bakanae disease in rice plants. This study aimed to obtain rhizobacteria isolates from the roots of rice plants and to determine their characteristics as biocontrol agents against F. fujikuroi in vitro. This research consisted of two stages: 1) Exploration of rhizobacteria from the roots of rice plants in Padang City, West Sumatera, Indonesia and 2) Antagonistic character of Rhizobacteria against F. fujikuroi in vitro including: antagonistic test, hydrolytic enzyme production test (protease, amylase, and chitinase), and HCN compound production test. Based on the research, 32 rhizobacterial isolates were obtained as candidates for biological agents. All rhizobacterial isolates can suppress the growth of Fusarium fujikuroi in vitro with an inhibition percentage of 19.43-73.53%. The 4 best isolates in suppressing the growth of Fusarium fujikuroi are Kr 2.2 from Kuranji, KG 2.2 from Korong Gadang, PA 2.1 and PA 1.2 from Pasar Ambacang with an inhibition percentage of 60.39-73.53%. The four isolates are also able to produce hydrolysis enzymes (protease, amylase and chitinase) and HCN compounds
Endophytic Bacteria Consortia as Biocontrol Against Brown Spot Disease (Helminthosporium oryzae) and Growth Promotion in Rice Eri Sulyanti; Zurai Resti; Haliatur Rahma; Darnetty Darnetty; Ujang Khairul; Nurhaliza Nurhaliza; Silvia Oktaviani
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 9 No. 1 (2025): June 2025
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.9.1.1-13.2025

Abstract

The brown spot disease (BSD) caused by Helminthosporium oryzae in rice can lead to yield losses of up to 45 %. Using endophytic bacterial consortia as a biological agent is one of the most potential control methods for resisting the disease. This study aimed to obtain an endophytic bacterial consortium with the best efficacy in controlling BSD and its ability to enhance rice plant growth. Statistical analysis in this study was made using Analysis of Variance (ANOVA) with Duncan’s Multiple Range Test (DMRT) in a randomized experimental design comprising five treatments with five replications. The treatments included: A (Serratia marcescens ULG1E4 + S. marcescens JB1E3), B (S. marcescens ULG1E4 + S. marcescens JB1E3 + Serratia marcescens JB1E2), C (Bacillus sp. SJI + Serratia marcescens ULG1E4), D (positive control), and E (negative control). Testing was conducted in planta, with the introduction of the bacterial consortium (10⁸ cells/mL) via seed and root soaking methods. Subsequently, the leaves of 30-day-old rice plants were inoculated with H. oryzae by spraying a conidial suspension of 10⁶ conidia/ml. Parameters observed included disease progression (incubation period, incidence, and severity), seedling growth metrics (height, leaf number, root length, fresh and dry biomass), and overall plant development (plant height, leaf number, and tiller count). The results demonstrated that the consortium of Serratia marcescens ULG1E4, Serratia marcescens JB1E3, and Serratia marcescens JB1E2 effectively controlled BSD, achieving a 53.69% reduction in disease severity and promoted rice plant growth, evidenced by a 30.63% increase in leaf number
The Effect of Entomopathogenic Fungi Combinations on the Growth and Production of Corn ( Zea mays L.) Indri Yanil Vajri VAJRI; Trizelia; Haliatur Rahma; Yusniwati
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 8 No. 2 (2026): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 8 No. 2 Mei 2026
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v8i2.5434

Abstract

Entomopathogenic fungi (CEPs) possess dual potential as biological control agents and endophytes that modulate plant physiological processes. Single applications of CEPs often provide suboptimal protection and growth support, necessitating a synergistic, interspecies combination approach. This study aimed to evaluate the effects of single and combined inoculations of CEPs on the growth, chlorophyll content, and yield components of shelled corn (Zea mays L.). A Randomized Block Design (RBD) with eight treatments and four replications was employed. The treatments included Beauveria bassiana (BBWS), Metarhizium anisopliae (B22C), and Trichoderma asperellum (A116), applied singly or in combination via seed soaking for six hours. Sterile distilled water served as the control. Observed parameters included plant growth, leaf chlorophyll content, and yield components. Data were analyzed using Analysis of Variance (ANOVA), followed by the Least Significant Difference (LSD) test at the 5% significance level. Results indicated that fungal inoculation had no significant effect on corn vegetative growth six weeks after planting. However, the combination of B. bassiana and M. anisopliae produced the highest total chlorophyll content (1,101 μg g⁻¹), whereas single inoculations decreased chlorophyll levels, likely due to carbon costs during the initial phase of endophyte colonization. Importantly, the combination of M. anisopliae and T. asperellum yielded the highest number of kernels per ear, ear weight, and 100-kernel weight. This research advances previous studies by demonstrating that synergistic fungal combinations effectively mitigate early-phase carbon costs, thereby maximizing crop yields compared to single applications. Therefore, the combination of M. anisopliae and T. asperellum is recommended as a biofertilizer formula and biocontrol agent for sustainable corn kernel production.
DISEMINASI TEKNOLOGI PUPUK KANDANG SAPI PLUS RIZOBAKTERI PADA KELOMPOK TANI KAKAO DI KABUPATEN LIMAPULUH KOTA Haliatur Rahma; Jumsu Trisno; Martinius Martinius; Reflin Reflin; Sri Wahyuni; Nusyirwan Nusyirwan
Jurnal Hilirisasi IPTEKS Vol. 1 No. 4.a (2018)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kegiatan diseminasi teknologi pupuk kandang sapi plus rizobakteri pada tanaman kakao dilakukan di Kecamatan Guguak dan Akabiluru Kabupaten Lima Puluh Kota yang dimulai dari bulan Maret sampai dengan November 2018. Kegiatan ini bertujuan untuk mengenalkan teknologi formulasi pupuk kandang plus rizobakteri pada kelompok tani kakao. Inovasi, buah lobek, maju sejahtera dan aroma menggunakan metode penyuluhan. Pentingny kegiatan ini dilakukan disebabkan oleh tingginya kejadian dan keparahan penyakit VSD di Kecamatan Guguak dan Akabiluru Kabuparten Limapuluh Kota yang mencapai 86,60%. Untuk itu, diperlukan teknologi pengendalian yang mudah dibuat, murah dan bahan baku yang mudah diperoleh disekitar petani. Penggunaan air kelapa merupakan salah satu perbanyakan bakteri Serratia maresescens dan Pseudomonas fluorescens yang nanti akan diformulasi dengan pupuk kandang. Kegiatan ini meliputi: pemantauan tingkat serangan OPT dilahan kelompok tani kakao, kemudian dilanjutkan dengan penyuluhan, pelatihan, dan evaluasi hasil kegiatan. Dari hasil kegiatan bahwa kegiatan ini mampu meningkatkan pengetahuan petani terhadap penggunaan biofertilizer yang berasal dari bakteri yang diformulasikan dengan air kelapa dan pupuk kandang, sehingga penggunaan formulasi ini mampu mengurangi biaya penggunaan pupuk kimia yang memiliki harga yang mahal.
PEMANFAATAN AGEN HAYATI UNTUK PENGELOLAAN OPT CABAI PADA KELOMPOK TANI SIMABUR SUKSES MAKMUR DI KECAMATAN PARIANGAN KABUPATEN TANAH DATAR Trizelia Trizelia; Nurbailis Nurbailis; Yulmira Yanti; Winarto Winarto; Haliatur Rahma; Martinius Martinius; Yenny Liswarni; Rusdi Rusli; Yunisman Yunisman; Darnetty Darnetty; Eri Sulyanti
Jurnal Hilirisasi IPTEKS Vol. 2 No. 3.b (2019)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v2i3.b.331

Abstract

Cabai merah merupakan sumber pendapatan utama bagi petani di daerah Tanah Datar. Masalah utama yang selalu dihadapi oleh petani cabai di daerah ini adalah serangan hama dan penyakit yang dapat menyebabkan kehilangan hasil yang cukup tinggi. Hingga saat ini, pengendalian hama dan penyakit sayuran masih mengandalkan pestisida sintetik yang telah menimbulkan dampak negatif. Untuk itu, perlu dicari alternatif pengendalian yang dapat mengurangi dampak negatif pestisida tersebut, yaitu menggunakan agens hayati seperti cendawan Beauveria bassiana dan Trichoderma sp.Tujuan dari kegiatan pengabdian kepada masyarakat ini adalah untuk memperkenalkan dan memasyarakatkan pengendalian hama dan penyakit menggunakan agens hayati Beauveria bassiana dan Trichoderma sp. Metode kegiatan pengabdian adalah penyuluhan, pelatihan dan praktek lapangan. Minat petani untuk menggunakan agens hayati terutama Beauveria bassiana dan Trichoderma cukup tinggi dan telah memotivasi mereka untuk mengurangi penggunaan pestisida sintetis. Petani juga telah berhasil memperbanyak agens hayati dan membuat kompos limbah pertanian menggunakan Trichoderma.
PENGGUNAAN TRICHODERMA SPP UNTUK PENGENDALIAN PENYAKIT DAN PENINGKATAN PERTUMBUHAN TANAMAN CABAI DI NAGARI TAEH BARUAH KECAMATAN PAYAKUMBUH KABUPATEN LIMAPULUH KOTA Yulmira Yanti; Nurbailis Nurbailis; Hasmiandy Hamid; Trizelia Trizelia; Haliatur Rahma; My Syahrawati; Noveriza Hermeria
Jurnal Hilirisasi IPTEKS Vol. 4 No. 1 (2021)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v4i1.491

Abstract

Jorong Parit Dalam Nagari Taeh Baruah, Payakumbuh District, Limapuluh Kota Regency is one of the villages that has various problems such as drought, high number of idle lands and remote access. The main crop planted during the rainy season is lowland rice, but most of the land becomes idle land during the dry season. In terms of fertilization, farmers in this location still use artificial fertilizers (chemical fertilizers) and in terms of pest and disease control they still use chemical pesticides. The solutions that can be offered are planting chili plants which are more resistant to drought, processing agricultural waste into compost as an alternative to reduce dependence on the use of chemical fertilizers, use of Trichoderma and vegetable pesticides as agents for controlling pests and diseases, and processing chili production to increase value. selling, competitiveness and community income. The purpose of this farmer group empowerment activity is to increase public knowledge about the use of Trichoderma which can increase the growth of chili plants, and can also be used as a biological agent to control pests and diseases that often attack chili plants when cultivated. The method used in this activity is socialization and counseling, structured training and direct practice in the field of Trichoderma's influence on the growth and production of chili plants. The results obtained from this activity are that the community can recognize that there are alternative controls that are more environmentally friendly than the use of synthetic pesticides. The public can also gain new understanding regarding the increased growth and yield of chili plants by utilizing the Trichoderma biological agent. So that in the end it can reduce dependence on synthetic pesticides obtained from outside. Therefore, the environment and the balance of the ecosystem in the Nagari Taeh Baruh area are maintained. The conclusion of this activity is that plant growth applied using Trichoderma spp is higher than plants that are not treated with Trichoderma spp (control). The use of Trichoderma spp is highly recommended because besides being easy to obtain, it also provides positive values for plant growth and is good for environmental technology around cultivated plants.
DISEMINASI PGPR SEBAGAI BIOAKTIVATOR DEKOMPOSISI BAHAN ORGANIK DI KELOMPOK USAHA TANI NAGARI SUNGAI ABANG KECAMATAN LUBUK ALUNG KABUPATEN PADANG PARIAMAN Haliatur Rahma; Indra Dwipa; Agustian Agustian; Yaherwandi Yaherwandi; Reflinaldon Reflinaldon; Jumsu Trisno; Armansyah Armansyah; Gusmini Gusmini; Afrima Sari; Ryan Dwi Setyawan; Rika Hariance; Dede Suhendra
Jurnal Hilirisasi IPTEKS Vol. 6 No. 2 (2023)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v6i2.625

Abstract

Nagari Sungai Abang is one of the areas in Lubuk Alung District, one of the rice production centers in Padang Pariaman Regency. One farming group that intensively cultivates rice is the Farming Business group. The problem with the farmer groups is their ignorance of the benefits of straw as a source of organic matter in the soil. The composting process is long, so farmers always burn rice straw after harvest in preparation for planting the following season. If the burning of straw is carried out continuously, it is feared that nutrients will be deficient in the soil. This activity aims to empower Farming Business groups in Nagari Sungai Abang, Lubuk Alung District, to utilize Plant Growth Promoting Rhizobacteria (PGPR) as a bio activator in making straw compost as organic fertilizer and an alternative to addressing the problem of straw waste in Nagari Sungai Abang, Lubuk Alung. This activity has been carried out in the Farming Group of Farmers in Sungai Abang Dalam Nagari Sungai Abang, Lubuk Alung District, Padang Pariaman Regency. The method used is outreach, counseling, and training to demonstrate making PGPR suspension and compost from straw. From the activities that have been carried out, ready-to-use PGPR is obtained as a bio activator reproduced in coconut water media and used as a bio activator to speed up the composting process. The conclusion of this activity is increasing farmers' understanding of the benefits of straw as a raw material for compost and the use of PGPR as a bioactivator in making straw compost.
Potential of culture filtrate from Trichoderma spp. as biofungicide to Colletotrichum gloeosporioides causing anthracnose disease in chili Nurbailis Nurbailis; AKMAL DJAMAAN; HALIATUR RAHMA; YENNY LISWARNI
Biodiversitas Journal of Biological Diversity Vol. 20 No. 10 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201020

Abstract

Abstract. Nurbailis, Djamaan A, Rahma H, Liswarni Y. 2019. Potential of culture filtrate from Trichoderma spp. as biofungicide to Colletotrichum gloeosporioides causing anthracnose disease in chili. Biodiversitas 20: 2915-2920. Trichoderma spp. have the potential to be used for controlling the airborne pathogenic fungi such as C. gloeosporioides. The purpose of this study was to evaluate the antifungal activity of the culture filtrate of five isolates of Trichoderma spp. (T. harzianum, T. viride, T. koningii, Trichoderma PP2, Trichoderma PP3) against C. gloeosporoides causing anthracnose disease in chili. Culture filtrate of Trichoderma spp. was produced from single culture and dual culture techniques. The design was a Completely Randomized Design with six treatments and four replications. The treatments were culture filtrate from T. harzianum, T. viride, T. koningii, Trichoderma PP2, Trichoderma PP3, and negative control (without culture filtrate from Trichoderma spp.). Parameters observed were: the diameter of the colony, colony coverage, conidial germination, and conidial density. The results of this research showed all the culture filtrate from Trichoderma spp. produced by single and dual culture techniques can inhibit the growth of C. gloeosporioides. The culture filtrate from Trichoderma PP2 and T. koningii were the most potential in inhibiting the growth, conidial density, and conidial germination of C. gloeosporioides.
Characterization and potential of plant growth-promoting rhizobacteria on rice seedling growth and the effect on Xanthomonas oryzae pv. oryzae Haliatur Rahma; NURBAILIS; NILA KRISTINA
Biodiversitas Journal of Biological Diversity Vol. 20 No. 12 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201226

Abstract

Abstract. Rahma H, Nurbailis, Kristina N. 2019. Characterization and potential of plant growth-promoting rhizobacteria on rice seedling growth and the effect on Xanthomonas oryzae pv. oryzae. Biodiversitas 20: 3654-3661. Xanthomonas oryzae pv. oryzae (Xoo), a major limiting factor in rice production, and the use of resistant Xoo varieties have failed to control the bacterial pathogens as well as increased yield. It is due to the diversity in pathotypes, caused by environmental factors, the nature of resistant variety used, and gene mutation. The aims of this study were to select rhizobacterial strains with the potential of suppressing Xoo growth and promoting the growth of rice seedlings. This experiment was conducted in a completely randomized design (CRD) using seven rhizobacterial isolates selected through a dual culture test, with four replications. There were four isolates that potential in inhibiting the growth of Xoo, namely KJKB5.4, LMTSA5.4, Bacillus cereus AJ34, and Alcaligenes faecalis AJ14, with inhibition diameters greater than 11.50 mm. Rhizobacterial supernatant of 4 potential isolates has a zone of inhibition ranging from 12.25 to 24.00 mm. Four potential isolates were also able to solubilize phosphate, produce indole acetic acid (IAA) growth hormone, and siderophore, as well as enhance the growth of rice seedlings. Based on the nucleic acid sequencing of LMTSA5.4, KJKB5.4, and RK12 isolates were identified as Stenotrophomonas malthopilia strain LMG 958 (99.13%) accession NR 119220.1, Stenotrophomonas pavanii strain LMG 25348 (95.84%) accession NR 118008.1 and Ochrobactrum ciceri strain ca-34 (92.91%) accession NR115819.1.
Co-Authors , Giyanto . Giyanto . Gusmini . Reflinaldon Afrima Sari Agustian Agustian Agustian Agustian Akmal Djamaan Alfons Dwi Putra Butar-Butar Aprizal Zainal Armansyah Armansyah Arneti Arneti Beatrix Sofranes Napitupulu Darnetty Darnetty Dede Suhendra Dede Suhendra Elfitri Syahdia Eri Sulyanti Eri Sulyanti FADHILA RAHMI Fajar Akbar Fauzan Abid Hanif Nasution Hasmiandy Hamid Ika Listiana Indra Dwipa Indri Yanil Vajri Indri Yanil Vajri Indri Yanil Vajri Indri Yanil Vajri Indri Yanil Vajri Jhon Nefri Jumsu Trisno Liswarni, Yenny Magdalena Saragih Martinius Martinius Martinius MARTINIUS MARTINIUS Martinius Martinius Martinius Martinius Martinius Martinius Martinius Martinius Meity S Sinaga Meity S Sinaga, Meity S Memen Suharman, Memen Memen Surahman Munzir Busniah Mutiara Septu MY SYAHRAWATI My Syahrawati MY SYAHRAWATI My Syahrawati Nasution, Lita Nefri, Jhon NILA KRISTINA NILA KRISTINA Noveriza Hermeria Noveriza Hermeria Nurbailis Nurfitri Sari Nurhaliza Nurhaliza Nusyirwan Nusyirwan Nusyirwan Nusyirwan Nusyirwan Nusyirwan Putri, Wahyuni Eka Rahma, Zola Puti Reflin Reflin Reflin Reflin Reflin Reflin Retna Astuti Kuswardani Ridho, Muhammad Arif Rika Hariance Rika Hariance RIKI WARMAN RITA NOVERIZA Rita Noveriza Rita Noveriza Rusdi Rusli Rusdi Rusli Ryan Dwi Setyawan Ryan Dwi Setyawan Ryan Hidayat Saipul Sihotang Sandra Desiska Silvia Oktaviani Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Yuliani Sri Yuliani Sufia, Yolanda Sulyanti, Eri Sulyanti, Eri Suryati . Tre Julia Nasral Trizelia UJANG KHAIRUL Wahyuni Eka Putri Winarto Winarto Winarto Winarto Winarto Winarto Yaherwandi Yaherwandi Yaherwandi Yulmira Yanti YUMBA LARASATI YUNISMAN . Yunisman Yunisman Yusniwati Yusniwati Zola Puti Rahma Zurai Resti