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Journal : Journal of Aquaculture and Fish Health

Motile Aeromonas Septicemia (MAS) Disease Resistance Test by Aeromonas hydrophila on Triploid Striped Catfish (Pangasianodon hypopthalmus) Hartami, Prama; Ayuzar, Eva; Salamah , Salamah; Nurjannah, Lilis; Carman, Odang; Alimuddin , Alimuddin; Rafi, Muhammad; Fakhri, Muhammad
Journal of Aquaculture and Fish Health Vol. 13 No. 1 (2024): JAFH Vol. 13 No. 1 February 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i1.45431

Abstract

Motile Aeromonas Septicemia (MAS) disease caused by Aeromonas hydrophila is a common pathogen that attacks freshwater fish commodities, including triploid as the new strain of striped catfish. This study aimed to test the resistance of triploid striped catfish to MAS infection before being released to farmers as a new commodity to increase national striped catfish production. The test was carried out for two months at the Sukamandi Fish Breeding Research Institute, Subang by injecting A. hydrophila into triploid and diploid striped catfish. The challenge test was carried out by injecting the cultured A. hydrophila with a density of 107 cfu.mL-1 as much as 0.1 mL.ind-1 at fish intramuscularly in all treatments, while in the Control (-) treatment, 0.1 mL of PBS solution was injected.tail-1. The test striped catfish used for each treatment were 10 individuals measuring 9.23 – 9.65 cm, and weighing 7.1 – 9.23 g. Survival parameter data and blood description were analyzed quantitatively using Microsoft Excel 2010 and SPSS version 16.0 software with analysis of variance (ANOVA) at a 95% confidence interval. The results of re-characterization with biochemical tests showed that A. hydrophila were gram-negative, oxidase and catalase positive, motile, could fermentatively convert sugar, and could grow in blood media. The survival of triploid striped catfish after the test was 95%, total erythrocytes on day 7, and total leukocytes on the first day were significantly different from diploid striped catfish. Thus, it can be concluded that striped triploid striped catfish are more resistant to infection with A. hydrophila than diploid.
Effect of different sodium nitrate concentrations on the growth, biomass, and biochemical composition of Tetraselmis chuii Fakhri, Muhammad; Amrulloh, Albazi Achmad; Yuniarti, Ating; Supriatin, Febriyani Eka; Arifin, Nasrullah Bai
Journal of Aquaculture and Fish Health Vol. 14 No. 1 (2025): JAFH Vol. 14 No. 1 February 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i1.64564

Abstract

Nitrogen plays a significant role in the growth and metabolism of microalgae. The purpose of this study was to investigate the influences of different sodium nitrate concentrations on growth, biomass, and biochemical profile in Tetraselmis chuii. Four different nitrate concentrations, 0.5, 1.0, 1.5, and 2.0 g/L NaNO3 were applied in T. chuii culture under a batch system. It was found that a low nitrate concentration of 0.5 g/L NaNO3 produced the highest specific growth rate and biomass concentration of T. chuii. On the other hand, increasing nitrate concentration led to an increase in chlorophyll a+b and carotenoid in T. chuii, with the optimum nitrate concentration found at 1.5 g/L NaNO3. Under the nitrogen limitation condition, protein content was significantly decreased, but lipid and carbohydrate content were highly accumulated in the cells. This study provides a unique phenomenon that low nitrogen concentrations not only produce higher biomass but also accumulate high lipid and carbohydrate content.