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DIVERSITAS BAKTERI ENDOFIT PADA DAUN MUDA DAN TUA TUMBUHAN ANDALEH (Morus macroura miq.) Dwi Hilda Putri; Mades Fifendy; Moca Faulina putri
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 19 No. 1 (2018): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (832.934 KB) | DOI: 10.24036/eksakta/vol19-iss1/122

Abstract

Endphytic microbes are microscopic living organisms (bacteria and fungi) that live in plant tissues (xylem and phloem), leaves, roots, fruit, and stems. These microbes live symbiosis of mutual benefit. Morus macroura miq. or Andaleh is a native plant of western Sumatra that is considered rare. The purpose of this study was to isolate and identify entophytic bacteria found in young leaves and old leaves andaleh taken from the village Andaleh, Kec. Batipuah West Sumatra. The methods used to isolate endophytic bacteria from andaleh leaves are spread plate and streak plate. Identification of endophytic bacteria in macroscopic or colony morphology. A total of 12 isolates of endophytic bacteria from young andaleh leaf and 8 isolates of endophytic bacteria from andaleh leaf. Identification Microscopic using gram staining obtained bacteria 18 gram positive endophytic bacterial isolates and 2 isolate gram negative bacteria.
PELATIHAN HOMEMADE PEPAYA SOAP BAGI KELOMPOK IBU PRODUKTIF DI KENAGARIAN SALIBUTAN KECAMATAN LUBUK ALUNG KABUPATEN PADANG PARIAMAN Des M; Mades Fifendy; Elsa Yuniarti; Rezi Junialdi
BERNAS: Jurnal Pengabdian Kepada Masyarakat Vol 2 No 4 (2021)
Publisher : Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.828 KB) | DOI: 10.31949/jb.v2i4.1476

Abstract

Nagari Salibutan has an agricultural typology. In addition to rice fields and forests, Nagari has a fairly large plantation of 500 hectares. One of the garden products produced by Nagari Salibutan is Papaya Fruit. The number of family heads in Kenagarian Salibutan is 512 families, consisting of 374 families of prosperous families and 138 families of underprivileged families. Of the number of families aged 18-56 years who are housewives as many as 42 people. So far, housewives are synonymous with taking care of the family at home, even though housewives can do other productive activities to help family income. One of the productive activities that housewives can do is make papaya soap. Harvested papayas are generally sold at a price of Rp. 2,000 to Rp. 6,000/kg. At the time of harvesting, the price of papaya tends to fall due to the abundance of papaya fruit, meanwhile there is no use of papaya fruit for direct consumption. In order for papaya fruit to have a high economic value, it is necessary to innovate to produce other selling products. One of the products produced from papaya fruit is soap. The soap produced by papaya is healthy because of the natural manufacturing process, this soap can be used as an original souvenir of the Salibutan Kenagarian. Besides that, soap from papaya fruit during the current COVID-19 pandemic, can be used by the public to wash their hands frequently. From the analysis of the situation above, the people in Kenagarian Salibutan need to be equipped with knowledge about the use of Papaya Fruits into products of economic value such as soap, so that people do not only sell papayas at low prices.
META ANALISIS PENGARUH METODE MIND MAPPING TERHADAP KETERAMPILAN BERPIKIR KREATIF SISWA Multi Asmul Sovia; Ganda Hijrah Selaras; Mades Fifendy
BIOCHEPHY: Journal of Science Education Vol. 3 No. 1 (2023)
Publisher : MO.RI Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52562/biochephy.v3i1.554

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui hasil analisis penggunaan metode mind mapping dalam meningkatkan keterampilan berpikir kreatif siswa. Metode penelitian ini menggunakan deskriptif analisis, teknik pengumpulan data menggunakan penelitian meta-analisis yaitu penelitian yang dilakukan cara pengulangan dan menganalisis penelitian dari beberapa hasil penelitian sebelumnya. Pengumpulan data dalam penelitian ini dilakukan dengan menelusuri artikel, jurnal elektronik, dan esai menggunakan "metode mind mapping", "keterampilan berpikir kreatif". Hasil pencarian menunjukkan bahwa ada banyak sekali penelitian tentang ini, tetapi penulis hanya mengambil data 10 artikel, jurnal elektronik, dan esai relevan untuk menunjang penelitian ini. Data dianalisis menggunakan analisis deskriptif kualitatif. Berdasarkan data penelitian dilakukan secara meta analisis, dapat disimpulkan bahwa penerapan metode mind mapping sudah banyak dilakukan oleh peneliti oleh sebelumnya. Hasil analisis menunjukkan bahwa metode mind mapping dapat meningkatkan keterampilan berpikir kreatif peserta didik. Hal tersebut diharapkan menjadi acuan guru supaya dapat menggunakan metode mind mapping dalam kegiatan belajar mengajar dalam pendidikan. Metode mind mapping sangat efektif untuk pembelajaran karena metode mind mapping terbukti dapat meningkatkan berpikir kreatif siswa menjadi lebih baik.
Pengembangan Lembar Kerja Peserta Didik (LKPD) Elektronik Berbasis Pendekatan Konstruktivisme pada Materi Sistem Sirkulasi untuk Peserta Didik SMA Vega Ersalina; Relsas Yogica; Suci Fajrina; Mades Fifendy; Asrul
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 10 No 2 (2023): Oktober 2023
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v10i2.20903

Abstract

21st Century Learning requires educators to be able to develop teaching materials in accordance with ICT developments and the needs of students. Electronic LKPD based on a constructivism approach to circulation system material for high school students was developed to help students become more interested and active in learning, understand material easily, and show the process of absorbing information so as to create meaningful learning. The type of research used is development research using the Intructional Development Institute (IDI) model which has 3 stages, namely define, develop, and evaluate. The subjects in this study were two lecturers of biologists at Padang State University, one biology educator and 30 students of class XI IPA at SMA Negeri 1 IV Koto. The object of this research is electronic LKPD based on constructivism approach on circulation system material for high school students. The research instruments used were educator interview questionnaires, student questionnaires, validity test sheets, and practicality test sheets. The results of the validity test of this electronic LKPD were 90.10% in the very valid category and the results of the practicality test of this electronic LKPD were 89.66% in the very practical category. It can be concluded that the electronic LKPD based on the constructivism approach on circulation system material for high school students gets very valid and very practical values.
Effect of Papaya Leaf Extract (Carica papaya L.) on Colony Diameter and Percentage of Growth Inhibition of Fusarium oxysporum Meriza Fatma; Moralita Chatri; Mades Fifendy; Dezi Handayani
Jurnal Serambi Biologi Vol. 6 No. 2 (2021): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

This study aimed to determine the effect of leaf extract concentration of C. papaya on the diameter of colonies F. oxysporum and the percentage of inhibition of growth of F. oxysporum. This research was conducted from February to April 2021 at the Integrated Research Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Padang State University. This study was an experimental study consisting of 5 treatments and 3 replications with the administration ofleaf extract at a C. papaya concentration of 0% (control), 10%, 20%, 30% and 40%. The data obtained were analyzed by means of variance (ANOVA) with Duncan's New Multiple Range Test (further testDNMRT)and the percentage of inhibition was analyzed descriptively. The results showed that leaf extract C. papaya at concentrations of 20%, 30% and 40% significantly affected the diameter of thecolonies F. oxysporum. The smallest colony diameter was at a concentration of 40% was 5.35 cm and the largest colony at a concentration of 10% was 6.61 cm. While the smallest percentage of inhibition at a concentration of 10% was 5% and the largest percentage of inhibition is at a concentration of 40% was 23%.
Test The Inhibition of Beringin Leaf Extract (Ficus benjamina L.) Against The Growth of Candida albicans in Vitro Santi Rodiah; Mades Fifendy; Gustina Indriati
Jurnal Serambi Biologi Vol. 7 No. 4 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

This study aimed to determine the inhibitory ability of F. benjamina leaf extract on the growth of the fungus C. albicans and the optimum concentration in inhibiting the growth of the fungus C. albicans. This research was conducted from October to December 2021 at the Microbiology Laboratory and Research Laboratory of the Department of Biology, Faculty of Mathematics and Science, Padang State University. This study is an experimental study consisting of 7 treatments and 3 repetitions with F. benjamina leaf extract at concentrations of 30%, 40%, 50%, 60%, 70% as well as negative control (sterile distilled water) and positive control (10% ketoconazole). Data for the diameter of the inhibition zone was analyzed by means of variance (ANOVA) and continued with further tests Duncan's Multiple Range Test (DMRT) further test. The results showed that the leaf extract of F. benjamina was able to inhibit the growth of the fungus C. albicans. The optimum concentration of F. benjamina leaf extract in inhibiting the growth of the fungus C. albicans at a concentration of 60% was 18.91 mm.
THE EFFECT OF VARIOUS CONCENTRATIONS OF ANTI-ACNE LIQUID SOAP ON THE BACTERIA OF Staphylococcus aureus CAUSES ACNE Delfi Suganda; Mades Fifendy; Linda Advinda
Jurnal Serambi Biologi Vol. 7 No. 4 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

his study aims to determine the effect of various concentrations of anti-acne liquid soap on Staphylococcus aureus and the interaction of the two types of liquid soap with their concentrations on the growth of S. aureus bacteria that causes acne. This research is an experimental study using a completely randomized design (CRD) in a 6 x 2 factorial with 3 replications. Factor A is liquid soap for acne, 2 levels are A1 = (Initial: P) , A2 = (Initial: C&C) Factor B is soap concentration, 6 levels are B1 = 2.5% (w/v), B2 = 5 % (w/v), B3 = 7.5% (w/v), B4 = 10% (w/v), B5 = 12.5% ​​(w/v), B6 ​​= Control (sterile distilled water). The results of this study indicate that the effect of various concentrations of anti-acne liquid soap on Staphylococcus aureus bacteria that causes acne is able to inhibit the growth of S. aureus, because the C&C brand of liquid anti-acne soap is able to form an average inhibition zone diameter of 1.94 cm at a concentration of 12, 5% while the anti acne P liquid soap brand was able to form an average inhibition zone diameter of 1.64 cm. From the statistical analysis data using the ANOVA test, the effect of various anti-acne liquid soaps on S.aureus had no significant effect and there was no interaction between the types of anti-acne liquid soap and its concentration on S.aureus which causes acne. Key words anti acne liquid soap, Staphylococcus aureus, concentration
Anti acne Solid Soap Antimicrobial Activity Test against Staphylococcus aureus Bacteria that Causes Acne Ade Indra Utama; Mades Fifendy; Linda Advinda
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The purpose of this study was to determine the antimicrobial activity of the anti-acne solid soap type and the effect of its concentration as well as the interaction between the anti-acne solid soap type and its concentration against Staphylococcus aureus bacteria that causes acne. This research was conducted from October - December 2021 at the Microbiology Laboratory, Faculty of Mathematics and Science, Universitas Negeri Padang. The methodology used in this study was an experiment with disc diffusion method using a completely randomized design (CRD) in a factorial consisting of 6 treatments and 3 repetitions with anti acne solid soap at concentrations of 2.5%, 5%, 7.5%, 10 %, 12.5% (w/v) and control (sterile distilled water). The inhibition zone diameter data were statistically analyzed using ANOVA, with = 5% and continued with Duncan's New Multiple Range Test (DNMRT) further test. The results of this study showed that both anti-acne solid soaps were able to provide antimicrobial activity against S. aureus bacteria at different concentrations because brand A and J solid anti-acne soap were able to form the highest average diameter of inhibition zones of 2.67 cm and 4, respectively. ,39 cm at 5% concentration. From the statistical analysis data using ANOVA, it was seen that the type and concentration of anti-acne solid soap had no significant effect on S. aureus bacteria and there was no interaction between the type of anti-acne solid soap and its concentration on S. aureus bacteria that causes acne.
Effect of Sungkai Leaf Extract (Peronema canescens J.) on Colony Diameter and Percentage of Growth of Inhibition Fusarium oxysporum Marisa Marisa; Moralita Chatri; Linda Advinda; Mades Fifendy
Jurnal Serambi Biologi Vol. 7 No. 3 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The purpose of this study was to determine the effect of the concentration of leaf extract P. canescens on colony diameter and the percentage of inhibition of growth of F. oxysporum. This research was conducted from November 2021 to December 2021, at the Integrated Research Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Padang State University. This study was an experimental study consisting of 5 treatments and 3 replications with leaf extract at P. canescens concentrations of 0%, 10%, 20%, 30%, 40%. The data obtained were analyzed by means of variance (ANOVA) with Duncan's New Multiple Range Test (DNMRT) further test. The results showed that the leaf extract of P. canescens . Effect on the diameter of the fungal colonies of F. oxysporum. The concentration of leaf extract affected the percentage of growth of inhibition F.oxysporum. The lowest percentage of inhibition was at a concentration of 10% and the highest at a concentration of 40%.
Resistance Test of Matoa Leaf Extract (Pometia pinnata J.R & G.Forst) On Fungi Growth of Candida albicans In-vitro Intan Rahma Putri; Mades Fifendy; Gustina Indriati
Jurnal Serambi Biologi Vol. 7 No. 4 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Fungal infection is one of the most common infectious diseases in the world, including Indonesia. Candidiasis is a dangerous disease, it is proven that the cases of death caused by candidiasis are in the range of 30-40% every year. Candidiasis is an infectious disease caused by fungi of the genus Candida, of which about 70% are caused by the species Candida albicans. Antifungal resistance can become a serious problem in the future. P. pinnata leaves are known to contain secondary metabolites, namely saponins, flavonoids, alkaloids, terpenoids and tannins. The purpose of this study was to determine the inhibition of P. pinnata leaf extract on the growth of the fungus C. albicans. This research was conducted using a completely randomized design (CRD) method with 6 treatments and 3 repetitions. The treatments were control using 10% ketoconazole and P. pinnata leaf extract (30%, 40%, 50%, 60%, 70%). The data that has been obtained were analyzed using ANOVA with a confidence level of = 5% and further tested with DNMRT. The results showed that P. pinnata leaf extract was able to inhibit the growth of the fungus C. albicans at concentrations of 70%, 60%, 50%, 40%, and 30% with an average diameter of the inhibition zones 10,10 mm, 11,62 mm, 12,78 mm, 12,34 mm, 10,97 mm.