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Bakteri Endofitik Penambat Nitrogen dan Teknik Aplikasinya untuk Pertumbuhan Tanaman Tomat (Solanum lycopersicum L.) pada Tanah Inceptisols Asal Jatinangor Mieke Rochimi Setiawati; Shafwah Zylvi Azkiannissa; Diyan Hermiyantoro; Reginawanti Hindersah; Andina Chotimah; Betty Natalie Fitriatin; Fasa Aditya; Pujawati Suryatmana
AGROLOGIA: Jurnal Ilmu Budidaya Tanaman Vol 13 No 2 (2024): Agrologia : Jurnal Ilmu Budidaya Tanaman
Publisher : Fakultas Pertanian, Universitas Pattimura Ambon, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/agrologia.v13i2.15637

Abstract

Inceptisols merupakan ordo tanah yang tersebar luas di Indonesia. Lahan Inceptisols menjadi pilihan yang potensial untuk kegiatan pertanian karena luasan totalnya yang cukup besar. Salah satu kendala dalam pemanfaatan tanah Inceptisols ialah sifatnya yang kurang subur dengan kandungan nitrogen (N) yang rendah. Solusi dari permasalahan tersebut salah satunya ialah pengaplikasian bakteri endofitik penambat N. Penelitian ini dilakukan dengan tujuan untuk menganalisis pengaruh inokulasi tiga jenis bakteri endofitik penambat nitrogen (Gluconacetobacter diazotrophicus corrig (GD1), G. diazotrophicus VTH-Ai80 (GD2), dan Paraburkholderia, sp.) dengan tiga teknik aplikasi yang berbeda (perendaman benih, penyiraman tanah, dan kombinasinya) terhadap pertumbuhan tanaman, populasi bakteri endofitik, dan bobot kering tanaman tomat pada tanah Inceptisols asal Jatinangor. Penelitian dilaksanakan di Rumah Kaca Percobaan Bale Tatanen dan Laboratorium Biologi Tanah Departemen Ilmu Tanah dan Sumber Daya Lahan, Fakultas Pertanian, Universitas Padjadjaran Jatinangor, Kabupaten Sumedang, Jawa Barat pada bulan Oktober s.d. Desember 2023. Rancangan percobaan yang digunakan yaitu Rancangan Acak Kelompok (RAK) yang terdiri 10 kombinasi perlakuan dengan 3 ulangan dan 3 subsampel. Hasil penelitian menunjukkan bahwa pemberian bakteri endofitik penambat N dengan teknik aplikasinya berpengaruh nyata pada tinggi tanaman 1 MST dan bakteri endofitik bagian akar, tetapi tidak berpengaruh nyata pada pengamatan lainnya. Perlakuan G. diazotrophicus VTH-Ai80 + Perendaman Benih + Penyiraman Tanah merupakan perlakuan yang lebih baik dalam meningkatkan tinggi tanaman 1 MST. Perlakuan G. diazotrophicus corrig + Penyiraman Tanah merupakan perlakuan yang lebih baik dalam meningkatkan populasi bakteri endofitik pada bagian akar tanaman tomat. Perlakuan G. diazotrophicus VTH-Ai80 + Penyiraman Tanah merupakan perlakuan terbaik dalam peningkatan indeks klorofil daun tanaman tomat.
Path analysis of phosphorus dynamics and chili response to low rank coal-derived humic acid in P-deficient Inceptisols Hermawan, Mega Kartika; Sofyan, Emma Trinurani; Sudirja, Rija; Nurbaity, Anne; Fitriatin, Betty Natalie; Mulyani, Oviyanti; Setiawati, Mieke Rochimi; Djuansah, Muhamad Rahman
Journal of Degraded and Mining Lands Management Vol. 13 No. 2 (2026)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2026.132.9893

Abstract

Jatinangor Inceptisols are formed from volcanic material with a high concentration of iron oxide and a low concentration of phosphorus (P), which leads to high rates of P fixation and low P fertilization efficiency. The situation is exacerbated by intensive agricultural practices. This research was conducted to assess the effect of humic acid derived from low rank coal (AHL) as a soil conditioner in enhancing P availability and chili response in P-deficient Inceptisols. The experiment was conducted in the greenhouse of the Soil Chemistry and Plant Nutrition Laboratory at Universitas Padjadjaran. AHL treatments were combined with various doses of SP-36 fertilizer to evaluate their direct and indirect effects on soil chemical properties, phosphate-solubilizing bacteria (PSB) populations, P uptake, and plant responses. The results showed that AHL application did not cause metal toxicity symptoms and significantly increased potential P, available P, cation exchange capacity (CEC), and total PSB populations. The best results were obtained with a combination of AHL at 30 kg ha?¹ and SP-36 at 125 kg ha?¹, which optimally enhanced P dynamics and chili yields. AHL was proven to reduce the need for inorganic P fertilizer by up to 50% without compromising chili yield or quality. These findings indicate that AHL has great potential to be developed as a locally sourced soil conditioner to support sustainable agriculture and the use of more environmentally friendly low-rank coal.
Effectiveness of Bacillus-Based Biofertilizer on Growth and Biomass of Corn in Low-Phosphorus Soil under Pot Experiment Reginawanti Hindersah; Betty Natalie Fitriatin; Aliya Zahrah Adawiah; Testarosa Testarosa; Yeni Wispa Dewi
AGROLOGIA: Jurnal Ilmu Budidaya Tanaman Vol 15 No 1 (2026): Agrologia : Jurnal Ilmu Budidaya Tanaman
Publisher : Fakultas Pertanian, Universitas Pattimura Ambon, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/agrologia.v15i1.22951

Abstract

Biofertilizers provide plants with essential nutrients through nitrogen fixation and phosphate solubilization. A pot experiment was conducted to examine the effects of different types of biofertilizers on plant growth, biomass, and rhizosphere microbial populations in corn grown in soil with low available phosphorus. The greenhouse experiment was designed using a completely randomized block design to test three biofertilizer types and two control treatments. The tested biofertilizers included a solid inoculant of phosphate-solubilizing bacteria, a liquid inoculant of a mixture of nitrogen-fixing bacteria and phosphate-solubilizing bacteria, and a Bacillus-based liquid biofertilizer. The results showed that soil inoculation with biofertilizer in soil enriched with organic matter did not alter leaf number or shoot height in 4-week-old corn; however, it increased both parameters compared to corn grown in soil without cow manure. Despite reduced root growth after biofertilizer inoculation, the corn dry weight was significantly higher following Bacillus-based biofertilizer application than with other biofertilizers. The experiment found that Bacillus was more effective for increasing corn biomass during vegetative growth in soil enriched with organic matter. The results indicate that the effectiveness of heterotrophic biofertilizer microbes is determined by soil organic matter.
Phosphate Uptake, Mycorrhiza Colonization and Yield of Soybean on Ultisols Affected by Water Stress and Mycorrhiza Fitriatin, Betty Natalie; Nurbaity, Anne; Suriadikusumah, Abraham
AGROTEK: Jurnal Ilmiah Ilmu Pertanian Vol 10, No 1 (2026): Maret
Publisher : Percetakan Umi Toaha Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/agrotek.v10i1.1176

Abstract

Mycorrhizal symbiosis can enhance nutrient uptake, especially phosphorus, by plants and increase plants’ drought resistance as well.  An experiment was set up to investigate the effects of water stress and mycorrhizal inoculation on phosphate uptake, mycorrhizal colonization, growth, and the yield of soybean.  The experiment design used was Factorial Randomized Block, which consisted of two factors. The first factor was water stress, which consisted of three levels, i.e., 100 % field capacity; 75 % field capacity, and 50 % field capacity.  The second factor was mycorrhiza, which consisted of three levels, i.e., without mycorrhizal inoculation, 10, and 20 g of mycorrhizal inoculant.  The experiment consisted of two units with three replications; the first unit was harvested at the end of the vegetative period to observe plant uptake of phosphorus, percentage of mycorrhizal infection, and dry weight of soybean, and the second unit was harvested at the end of the generative period to find out the yield of soybean. The results of this experiment showed that there was no interaction effect between water stress and mycorrhizal inoculation on plant uptake of phosphorus,  percentage of mycorrhizal infection, dry weight, and yield of soybean.  Water stress decreased phosphate uptake,  percentage of mycorrhizal infection, dry weight, and yield of soybean. Mycorrhizal inoculation increased phosphate uptake and the percentage of mycorrhizal infection.
The effect of beneficial microorganism as biofertilizer application in hydroponic-grown tomato Setiawati, Mieke Rochimi; Afrilandha, Nandha; Hindersah, Reginawanti; Suryatmana, Pujawati; Fitriatin, Betty Natalie; Kamaluddin, Nadia Nuraniya
SAINS TANAH - Journal of Soil Science and Agroclimatology Vol 20, No 1 (2023): June
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/stjssa.v20i1.63877

Abstract

The use of N2-fixing bacteria and growth hormone-producing rhizobacteria delivers nitrogen, enhances nutrients absorption by plants, and reduces the usage of inorganic fertilizers. Implementing biofertilizer in the hydroponic system as a means to reduce application of synthetic nutrient is recently in interest due to economic, food safety, and sustainability factors. This study determines the effects of biofertilizer dose on tomato yields in the hydroponics system. A randomized block design was utilized that consisted of seven treatments, namely 100% inorganic fertilizer and 0% biofertilizer (control), and various doses of inorganic nutrient combined with 25%, 50%, 75%, and 100% biofertilizer. The result illustrated that the application of biofertilizer augmented the population of endophytic bacteria, Azotobacter sp., Azospirillum sp., phosphate solubilizing bacteria, and nitrogen content. The distinct combination of biofertilizer did not alter the phosphorus and potassium content compared to control samples however resulted in superior tomato grade. The 50% inorganic fertilizer and 100% biofertilizer combination amplified the weight of the fruit by 36% compared to the control. This finding indicates that the application of biofertilizers in the hydroponic system for tomato plants is not only beneficial in minimizing the dosage of inorganic fertilizers but also enhancing the fruit quality.
Co-Authors Abraham Suriadikusumah Ade Setiawan Adinda Putri Amanda Aditya, Fasa Aditya, Fasa Aditya, Fasa Afrilandha, Nandha Agus Wahyudin Alifa Salsabila Anindya Aliya Zahrah Adawiah Alyani Shabrina Anas Ramdhani Andi Hana Mufidah Elmirasari Andina Chotimah Andriana Kartikawati Anggi Jingga Anggi Jingga Anne Nurbaity Anni Yuniarti Anny Yuniarti Antonio Yusuf Ariani, Nabila Syifa Aristyo Rahardiyan Asmiran, Priyanka Azhari, Yolanda Dewi Azwari, Fachruddin Budiman, Muhammad Nafariz Chotimah, Andina Damayani, Maya Dedi Nursyamsi Dedi Nursyamsi Dedi Nursyamsi Dedi Ruswandi Dedy Nursyamsi Dewi, Yeni Wispa Dirga Sapta Sara Dita, Limbong Agatha Dita Diyan Herdiyantoro Diyan Hermiyantoro Djuansah, Muhamad Rahman Dupa, Putri Siska Ekayanti Dwi Suci Rahayu Dzakaria Yogaswara Dzakaria Yogaswara Emma Trinurani Sofyan Endo, Kenji Fadhilla Oktavianingtyas Trisilvi Fakhrurroja, Hanif Fakhrurroja, Hanif Fakhrurroja Fasa Aditya Fauziah, Nicky Oktav Feni Siti Fauziah2 Fidya A Puspafirdausi Fiky Yulianto Wicaksono Fiqriah Hanum Khumairah Fiqriah Hanum Khumairah Fitri Widiantini Ghifari, Raden Faqih Hilmiy Haryo Probo Kusumo Hermawan, Mega Kartika Iis Nur Asyiah Khumairah, Fiqriah Hanum Leoni Silvia Maharani, Nadhira Saniya Mahdi Argawan Putra Manurung, Dahlia Florencia Marenda Ishak Sonjaya Sule Maulana, Nurzen Mayang Agustina Meddy Rachmady Mieke Rochimi Setiawati Mohamad Dion Tiara Muhamad Aristiyo Muhamad Aristiyo, Muhamad Muhamad Iqbal Kusma Sudana Muhammad Agi Pratama Muhammad Amir Solihin Muhammad Iqbal Maulana Yusuf Mulya, Agus Surya Mulyadi Mulyadi Muthia Erwina Nabila Nabila Syifa Ariani Nadia Nuraniya Kamaluddin Nakayama, Masakazu Nono Carsono Nugraha, Gita Bina Nugraha, Gita Bina Nugraha, Gita Bina Nurfitriana, Nofalia Nurul Hakim, Anisa Oktaviani, Meira Oviyanti Mulyani Pirda Nurhopipah Priyanka Asmiran Pudjawati Suryatmana Pujawati Suryatmana Pujawati Suryatmana Puspafirdausi, Fidya A. Putri, Envry Artanti Duidahayu Rachelita, Nadia Randy Nugraha Rangga Jiwa Wibawa Reginawanti Hindersah Ria, Elly Roosma Rija Sudirja Risanti, Rara Rahmatika Risanti, Rara Rahmatika Rita Harni Robi Natalie Silpanus Ruminta Ruminta Septyani Sofatin Setiawati, Mieke Richimi Shafwah Zylvi Azkiannissa Simanjuntak, Edo Kelvin Syifa Nabila Kurnia Testarosa Testarosa Tien Turmuktini Toto Bustomi Triani Dewi Triyani Dewi Tualar Simarmata Wahyuda Rachman, Wahyuda Wahyudin, Agus Wawan Rachman Wong, Mui-Yun Yanti Ningtyas, Dewi Nurma Yeni Wispa Dewi Yenny Muliani yeti kartika Yuhanidz Yuhanidz Yuliati Machfud