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All Journal HAYATI Journal of Biosciences VALENSI Microbiology Indonesia Indonesian Journal of Pharmaceutical Science and Technology Jurnal Farmasi Klinik Indonesia Journal of Medicine and Health Chimica et Natura Acta JURNAL SAINS INDONESIA Jurnal Pendidikan Kimia (JPKim) Communications in Science and Technology Medika Kartika : Jurnal Kedokteran dan Kesehatan Jurnal Abdimas Kartika Wijayakusuma JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Jurnal Pengabdian Mandiri Acta Medical and Health Sciences (AMHS) Journal of Medicine and Health
KHOMAINI HASAN
Faculty Of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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Journal : VALENSI

 1 
The Effect of Single Co-expression of The DnaK-DnaJ-GrpE and GroEL/ES Chaperones and Their Combination on Expression Intein-pretrombin-2 in Escherichia coli ER2566 Iman Permana Maksum; D Agus Yusuf Wildan; Khomaini Hasan; Toto Subroto
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 6, No. 1, May 2020
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (597.174 KB) | DOI: 10.15408/jkv.v6i1.11333

Abstract

The use of recombinant thrombin in the manufacture of fibrin glue allows diseases contamination to be avoided. However, the expression of recombinant protein in E. coli still has a disadvantage of the formation of inclusion bodies, so it needs to be minimized by co-expression of chaperones. Therefore, the aim of this study was to determine the effect of single DnaK-DnaJ-GrpE and GroEL/ES chaperone expression and their combination on the expression of intein-pretrombin-2Ti,pH on E. coli ER2566. The method started with isolation of pTWIN1-prethrombin-2Ti,pH and pG-KJE8 from E. coli TOP10F' and DH5α respectively, the co-transformation of the expression host E. coli ER2566 using pG-KJE8 and pTWIN1-prethrombin-2Ti,pHvectors, the chaperone co-expression was induced using L-Arabinosa before IPTG induction and cell culture growth was incubated at 22 oC. The expression products were characterized by using Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The results of the co-expression of chaperone showed that the number of soluble fraction was higher than the one without co-expression of chaperone. In addition, the co-expression of chaperone using pG-KJE8 in intein-prothrombin-2Ti,pH expression was sufficient using tetracycline as an inducer.
Co-Authors Achmad, Nurjannah Akmal Dhaffa Ulhaq Amallia Ardila Putri Andreanus Andaja Soemardji Andreas A Soemardji Andrian R, Andri Ania Kurniawati Purwa Dewi Arsyad Parama Santosa Audrey, Gabriella Budiantoro, Ogi Cicilia Liempapas D Agus Yusuf Wildan Daswara Djajasasmita Desi Veronika Pardosi DESSY NATALIA Dewi R Handayani Dian Anggraeny Eka Noneng Nawangsih Elly Noer Rochmah, Elly Noer Endry Septiadi Endry Septiadi et al Evi Sovia FERNITA PUSPASARI Gabriella Audrey Gabriella, Janifer Handayani, Dewi Ratih I Gede Eka Perdana Putra, I Gede Eka Perdana Iis I Rakhmat Iis Inayati Rahmat Ilma Fiddiyanti Iman Permana Maksum Iman Permana Maksum IMAN PERMANA MAKSUM Iman Permana Maksum Iman Permana Maksum IMAN PERMANA MAKSUM Inayati, Iis Indarti Trimurtini Indriyana, Sri Quintina IS HELIANTI Janifer Gabriella Liempapas, Cicilia Luvina, Petrisia Maria Ulfah Meilinah Hidayat MEILINAH HIDAYAT Mohammad Yusuf Muhammad Fadhil Z, Rusdi Noor Rosa (p:43-50) Murniaty Simorangkir Mutiara, Dinar N Ferdianti, Fine NIKNIK NURHAYATI Ogi Budiantoro OGI BUDIANTORO P Y Paryati, Sayu Pertiwi, Wulan Petrisia Luvina Purba Upay Ratunanda, Susanti Riezki Amalia Rini Roslaeni Sabbathini, Gabriela Christy Safari, Ardhi Ryanto SARONOM SILABAN Saronom Silaban Saronom Silaban Sayu Putu Yuni Paryati Sayu Putu Yuni Paryati Septiadi, Endry Shabarni Gaffar Shabarni Gaffar -, Shabarni Gaffar SHABARNI GHAFFAR Sigit Sasongko Sijani Prahastuti Sijani Prahastuti Sijani Prahastuti Siska Telly Pratiwi Soemardji, Andreas A Soetijoso Soemitro SOETIJOSO SOEMITRO Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Sutan Bagas Prakasa SUTARYA ENUS Sutarya Enus Sutarya Enus Sutarya Enus Toto Subroto Upay, Purba WANGSA TIRTA ISMAYA Welly Ratwita Wulan Pertiwi Wulandari, Sri Rezeki Yudith Y Kusmala Yudith Yunia K Yulistio Hanif