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Ujang Subhan
Fakultas Perikanan Dan Ilmu Kelautan Universitas Padjadaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Physics Public Health Social Sciences Other

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Journal : JURNAL BIOLOGI INDONESIA

 1 
Deteksi Keragaman Genotip Hibrid Ikan Lele Sangkuriang, Mutiara Transgenik dan Non Transgenik Pada Keturunan Pertama Buwono, Ibnu Dwi; Lathifah, Asri Ulfah; Subhan, Ujang
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.595 KB) | DOI: 10.14203/jbi.v14i1.3671

Abstract

ABSTRACTThe genotypic diversity showed by the hybrid crossbreed of transgenic Mutiara (carrying African catfish growth hormone) with non-transgenic catfish (Mutiara or Sangkuriang strain) high enough (many polymorphic fragments) in the first offspring. The aims of the study were to detect genotypic diversity from Sangkuriang catfish, transgenic Mutiara, non-transgenic Mutiara and first offspring (hybrid F1) with RAPD (Random Amplified Polymorphic DNA) marker using 2 primary types (OPA-03 and OPA-06). Experimental method is exploratively used in this research with qualitative descriptive analysis. The amplification results show the OPA-03 primer (5-AGTCAGCCAC-3 ) is the best primer that visualizes fragments (polymorphic and monomorphic) in all samples. The genetic relationship of the test fish using the NTSYSpc program shows that the OPA-03 phenogram in the first progeny of crossing the transgenic Mutiara male and Sangkuriang female has more genotypic diversity than other crosses. The first offspring of the broodstock crosses of the same strain (Sangkuriang and Sangkuriang) had a kinship of 70%, the crosses between non-transgenic Mutiara with Sangkuriang had 79% genetic similarity. The highest genetic similarity index (82%) was obtained from the first progeny of crossing transgenic Mutiara with Sangkuriang.Keywords: polymorphism, RAPD, phenogram, crossing, transgenesis
DETEKSI KERAGAMAN GENOTIP HIBRID IKAN LELE SANGKURIANG, MUTIARA TRANSGENIK DAN NON TRANSGENIK PADA KETURUNAN PERTAMA Buwono, Ibnu Dwi; Lathifah, Asri Ulfah; Subhan, Ujang
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3671

Abstract

ABSTRACTThe genotypic diversity showed by the hybrid crossbreed of transgenic Mutiara (carrying African catfish growth hormone) with non-transgenic catfish (Mutiara or Sangkuriang strain) high enough (many polymorphic fragments) in the first offspring. The aims of the study were to detect genotypic diversity from Sangkuriang catfish, transgenic Mutiara, non-transgenic Mutiara and first offspring (hybrid F1) with RAPD (Random Amplified Polymorphic DNA) marker using 2 primary types (OPA-03 and OPA-06). Experimental method is exploratively used in this research with qualitative descriptive analysis. The amplification results show the OPA-03 primer (5'-AGTCAGCCAC-3 ') is the best primer that visualizes fragments (polymorphic and monomorphic) in all samples. The genetic relationship of the test fish using the NTSYSpc program shows that the OPA-03 phenogram in the first progeny of crossing the transgenic Mutiara male and Sangkuriang female has more genotypic diversity than other crosses. The first offspring of the broodstock crosses of the same strain (Sangkuriang and Sangkuriang) had a kinship of 70%, the crosses between non-transgenic Mutiara with Sangkuriang had 79% genetic similarity. The highest genetic similarity index (82%) was obtained from the first progeny of crossing transgenic Mutiara with Sangkuriang.Keywords: polymorphism, RAPD, phenogram, crossing, transgenesis
Co-Authors Abi Muhamad Abdillah Afriza, Azka Reyza Aisyah Aisyah Al Mujahid, Muhammad Abdul Aziz Andry Tri Hermawan Ardhardiansyah - Asri Ulfah Lathifah Atikah Nurhayati Aufa, Muhammad Azri Ayi Yustiati Camellia Panatarani Darmawan, Mochamad Reza Darmawan, Reza Dermawan, Rifai Eddy Afrianto Elisah Fiyanih Emma Rochima Faisal Rizki Hadi Fenta Aquarista Fuaddin, Taufiq Gemilang Lara Utama Gilang Kesuma Mulyadi Gumay, Nadia Purnamasari Handaka, Asep Agus Handaka, Asep Agus Hardiono Tondang Herman, Roffi Grandiosa Heti Herawati I Made Joni Ibnu Bangkit Biosina Suryadi Ibnu Bangkit Biosina Suryadi Ibnu Dwi Buwono Ilyasa, Muhamad Diaz Indah Riyantini Ine Maulina Irfan Zidni Irmasari - Iskandar - Iskandar - Iskandar - Iskandar - Iskandar Iskandar Iskandar Iskandar Iwang Gumilar Kiki Haetami Kosasih, Henry Putra Kurniawati - Lathifah, Asri Ulfah Lathifah, Asri Ulfah Lintang Permata Sari Yuliadi M. Untung Kurnia Agung Mansyur Mansyur Marchelin, Shefia Meisani, Isni Mia Miranti Rustama Mochamad Rhandika Jayalaksana Muhamad Rakhman Firdaus Nugraha, Zidan Fachriza Adya Nuruhwati, Isni Perdana Putra Kelana Pratiwi Dinda Mutianugrah Rangga Bayu Kusuma Haris Rico ‎Rico Rita Rostika Rocella - Viernanda Roffi Grandiosa Roffi Grandiosa Rosidah - Rosidah - - Rosidah Rosidah Rusky Intan Pratama Ruth Mawar Ryan Adhitia Muslim Sukarman, Irwa Sukma bin W. Gunawan Walim Lili Wirawan Arief, Mochamad Candra Yayat Dhahiyat Yudi Nurul Ihsan Yuli Andriani Yuli Andriani Yuli Andriani Yulisa, Vira Azhari Yuniar Mulyani Zahidah - Zahidah Hasan Zahidah Zahidah, Zahidah Zahidah, , Zidni, Irfan