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All Journal Biotika: Jurnal Ilmiah Biologi Protobiont: Jurnal Elektronik Biologi Sainstek Biocelebes Bioeksperimen: Jurnal Penelitian Biologi Jurnal Perlindungan Tanaman Indonesia Science and Technology Indonesia Indonesian Journal of Chemistry JURNAL PENGABDI Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Jurnal Entomologi Indonesia Jurnal Informatika dan Rekayasa Elektronik

Kustiati Kustiati

Universitas Tanjungpura

Author-ID : 2691343

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Education Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics Social Sciences Other

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EFEKTIVITAS CENDAWAN ENTOMOPATOGEN METARHIZIUM ANISOPLIAE DALAM MEMBUNUH Imago Musca domestica L. (DIPTERA: MUSCIDAE) Florianus Flori; Noni Yunizar; Linawati Linawati; Kustiati Kustiati
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 2: September 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i2.8988

Musca domestica L. atau lalat rumah dianggap sebagai serangga pengganggu di bidang kesehatan dan merupakan vektor mekanis beberapa penyakit pada manusia dan ternak. Penelitian ini bertujuan untuk mengetahui keefektivitas cendawan Metarhizium anisopliae dalam membunuh imago Musca domestica. Lalat rumah yang digunakan adalah populasi lokal dari tempat pembuangan sampah di kota Pontianak. Metode penelitian dirancang mengunakan rancangan acak lengkap terdiri dari lima perlakuan dan satu kontrol dengan tiga ulangan yaitu: (P0) Kontrol; (P1) 1,2x konidia/ml;(P2) 3,6x106 konidia/ml;(P3) 6x106 konidia/ml;(P4) 8,4x106 konidia/ml; dan (P5) 10,8x106 konidia/ml diberikan dalam bentuk suspensi sebanyak 1 ml. Jumlah lalat yang menunjukkan paralisis dicatat dari satu hari setelah perlakuan hingga hari ke 14. Data kematian imago lalat dianalisa secara probit dengan bantuan program Excel untuk menentukan waktu kematian 50% (LT50) dari masing-masing konsentrasi perlakuan. Hasil penelitian menunjukkan bahwa suspensi M. anisopliae dengan kerapatan konidia 8x106 konidia/ml lebih efektif dalam membunuh imago M. domestica dalam waktu 5,88 hari dengan kisaran waktu mortalitas lalat uji sebesar 95%, yaitu 4,70–7,36 hari. Perlakuan dengan suspensi kerapatan konidia terendah mampu membunuh imago M. domestica L. sebanyak 50% atau lebih.

Efikasi Asap Cair dari Kayu Bengkirai terhadap Phytophthora citrophthora Hasan Ashari Oramahi; Elvi Rusmiyanto P. Wardoyo; Kustiati Kustiati
Jurnal Perlindungan Tanaman Indonesia Vol 22, No 2 (2018)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.33113

Bengkirai is one of kind of woods usually used for furnitures and produces lots of woodcuts. This woodcut become the raw material for liquid smoke. The efficacy of liquid smoke produced from bengkirai wood against Phytophthora citrophthora was evaluated. The aim of this research was to evaluate antimicrobial properties of liquid smoke from bengkirai wood against P. citrophthora. Three kinds of liquid smoke were used in three temperatures i.e., 350, 400 and 450ºC. Efficacy of liquid smoke from bengkirai wood for antimicrobial used PDA medium. Simple linear regression was used to measured the effect of liquid smoke concentration to inhibition of P. citrophthora growth. The relationship between the concentration of liquid smoke (X) and inhibition of fungal growth, P. citrophthora (Y) at 350, 400, and 450ºC were Y = 24.51 + 5.27X (r2 = 0.98), Y = 54.31 + 5.53X (r2 = 0,92), and Y = 51.32 + 16.87X (r2 = 0.80). The results showed that the concentration of liquid smoke was significantly different for inhibition of P. citrophthora growth. The higher the concentration the higher the inhibition of microbial growth. The highest inhibition was on liquid smoke of bengkirai wood with temperature pyrolysis of 450ºC and concentration of liquid smoke of 1% with average value of 100%. IntisariBengkirai merupakan bahan baku pembuatan furniture yang banyak menghasilkan limbah potongan kayu. Limbah potongan kayu ini dapat dijadikan sebagai sumber pembuatan asap cair. Penelitian tentang efikasi asap cair dari kayu bengkirai terhadap Phytophthora citrophthora telah dilakukan. Penelitian ini bertujuan untuk mengevaluasi sifat antimikrobia asap cair kayu bengkirai dalam menghambat pertumbuhan P. citrophthora secara in vitro. Tiga jenis asap cair kayu bengkirai hasil pirolisis pada suhu 350, 400, dan 450ºC. Medium PDA digunakan untuk efikasi asap cair kayu bengkirai terhadap pertumbuhan P. citrophthora. Analisis regresi linier sederhana digunakan untuk mengevaluasi pengaruh konsentrasi terhadap daya hambat pertumbuhan P. citrophthora. Konsentrasi asap cair kayu bengkirai yang digunakan adalah 0; 0,5; 1,0; 1,5 dan 2,0 %, v/v. Hasil penelitian menunjukkan bahwa hubungan antara konsentrasi asap cair (X) dan daya hambat pertumbuhan P. citrophthora (Y) pada suhu pirolisis asap cair 350, 400, dan 450ºC berturut-turut adalah Y = 24,51 + 5,27X (r2 = 0,98), Y = 54,31 + 5,53X (r2 = 0,92), dan Y = 51,32 + 16,87X (r2 = 0,80). Makin tinggi konsentrasi asap cair kayu bengkirai makin tinggi daya hambat terhadap pertumbuhan P. citrophthora. Daya hambat pertumbuhan P. citrophthora tertinggi (100 %) pada perlakuan asap cair kayu bengkirai yang diproduksi pada suhu pirolisis 450ºC dan konsentrasi 1 %.

Aktivitas Repelensi Senyawa Bioaktif dari Buah Makasar (Brucea javanica L. (Merr)) Terhadap Nyamuk Aedes aegypti Kustiati Kustiati
Biocelebes Vol. 1 No. 1 (2007)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Telah dilaksanakan penelitian tentang aktivitas repelensi senyawa bioaktif dari buah Makasar (Brucea javanica L. Merr) terhadap nyamuk Aedes aegypti. Penelitian ini bertujuan untuk mengisolasi, menentukan struktur dan menguji aktivitas repelensi senyawa bioaktif dari buah Makasar terhadap nyamuk Aedes aegypti.Proses isolasi dan pemurnian senyawa penolak nyamuk dimulai dengan maserasi menggunakan metanol dilanjutkan ekstraksi secara partisi dengan berbagai tingkat kepolaran pelarut serta pemurnian dengan teknik-teknik kromatografi. Elusidasi struktur dengan berbagai metode spektrometri, dilanjutkan uji aktivitas repelensi terhadap nyamuk Aedes aegypti menurut Tawatsin et al., (2001) dan Li & Ho (2000).Berdasarkan hasil penelitian menunjukkan bahwa fraksi n-heksan pada konsentrasi 3%, 4% dan 5% menunjukkan aktivitas repelensi tertinggi 100%, aktivitas ini lebih baik dari kontrol positif vanillin 5%. Isolat FMC3.2.2 menunjukkan spot noda tunggal yang diduga merupakan terpenoid dengan gugus lakton, pada konsentrasi 5% mempunyai aktivitas repelensi 77,77% yang berarti lebih rendah dari aktivitas repelensi kontrol positif vanillin 5% sebesar 87,50%. Analisis spektrometri isolat FMC3.2.2 menunjukkan UV λ maks (CH3OH) : 339 dan 220 nm. IR ν maks (KBr) cm-1: 3406,1 cm-1dan 1377,1 cm-1(OH); 1728,1 cm-1dan 1735 cm-1 (δ-lakton); 2923,9 cm-1dan 2854,5 cm-1(CH) dan 1172,6 cm-1(C-O-C).Key words: Brucea javanica L. Merr, aktivitas repelensi, Aedes aegypti

Keragaman Karakter Fenotipik Nyamuk Aedes di Daerah Endemis Demam Berdarah di Kalimantan Barat Kustiati Kustiati; Siti Ifadatin; Syarief Adiansyah
Biocelebes Vol. 5 No. 1 (2011)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Research on variety of phenotypic characters Aedes mosquito in dengue fever endemic area in West Kalimantan was conducted on July until December 2008. The research site ware fixed according to Stratified Random Sampling Method. The research was conducted by collating the morphological characters based on form, colour and amount of head, thorax and abdomen. Analysis of cluster using PCA (Principal Component Analysis). The inventory result found four species Aedes mosquito that are A. aegypti, A. albopictus, A. polynesiensis, and A. scuttelaris. Based on principal component analysis each mosquito larva, male and female have 9, 18 and 17 characters respectively determind for those four species of Aedes.

Catfish spawning training and silkworm cultivation in Pelita Jaya Village Indonesia Widadi Padmarsari Soetignya; Kustiati Kustiati
Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Vol. 5 No. 1 (2021)
Publisher : Universitas Ahmad Dahlan, Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jp.v5i1.2799

The catfish culture development program organized by the National Peatland Restoration Agency in 2017 in the Village of Pelita Jaya Indonesia, has a positive impact on the economy. However, there are several obstacles related to the problem of providing catfish juveniles and formulated feed. The Community Partnership Program (PKM) activity aims to increase the income of the â€œSenang Bersamaâ€ Fish Farmers Group through training in catfish spawning techniques and silkworm cultivation. The activity was held in the Village of Pelita Jaya, Kabupaten Kubu Raya, from May to September 2019. The methods used were lectures, demonstrations, training which included hormonal-induced catfish spawning techniques using ovaprim and sludge worm cultivation training. Monitoring and evaluation of activities are carried out starting from the first training until the activity ends. Activity evaluation was carried out using a retrospective (before-after) method using a questionnaire. The training results showed that the activity received a good response from PKM partners with the active participation of the partners and increasing the knowledge and skills of partners in the application of catfish spawning techniques and silkworm cultivation. The PKM partners could practice catfish spawning techniques and silkworm cultivation and apply them to fulfill the needs of group members in terms of catfish juveniles and feed. The impact of the activity as a whole is to develop the independence of the â€œSenang Bersamaâ€ Fish Farmers Group in providing juveniles and natural feed for catfish.

Status dan perkembangan resistensi Aedes aegypti (Linnaeus) (Diptera: Culicidae) strain Bandung, Bogor, Makassar, Palu, dan VCRU terhadap insektisida permetrin dengan seleksi lima generasi Yerslin Mantolu; Kustiati Kustiati; Trisnowati Budi Ambarningrum; Sri Yusmalinar; Intan Ahmad
Jurnal Entomologi Indonesia Vol 13 No 1 (2016): Maret
Publisher : Perhimpunan Entomologi Indonesia

Aedes aegypti (Linnaeus) (Diptera: Culicidae) is the main vector of dengue fever. In Indonesia, insecticides, especially pyrethroids, such as permethrin, have been effectively used to control Ae. aegypti. Notwithstanding that permethrin has been used since 1980s, information regarding the status and development of resistance of Ae. aegypti to permethrin is still limited and need further update. This study was conducted using the WHO standard test method. The aims was to determine the resistance status, and changes in resistance level of four field strains (Palu, Makassar, Bandung, Bogor) and susceptible strain (VCRU) of Ae. aegypti after the selection with permethrin for five generations. The results showed that resistance status of all field strains to permethrin were considered as high. The value of RR50 ranged between 10.5 to 25.7 fold. Bandung strain had the highest value of RR50 (22.5 fold), while Makassar strain had the lowest value of RR50 (10.5 fold). The fifth generation (F5) of five selected Ae. aegypti strains had the level of resistance 5 to 18 times higher than their parental. Knowledge of resistance status in a given area accompanied with the understanding about the development of resistance can be used to design a better vector management.

Resistensi lalat rumah, Musca domestica Linnaeus (Diptera: Muscidae) dari empat kota di Indonesia terhadap permetrin dan propoksur Intan Ahmad; Silvi Susanti; Kustiati Kustiati; Sri Yusmalinar; Resti Rahayu; Nova Hariani
Jurnal Entomologi Indonesia Vol 12 No 3 (2015): November
Publisher : Perhimpunan Entomologi Indonesia

House flies (Musca domestica Linnaeus) is one of the common urban pests causing health, economics, and aesthetics problems. Although intensive control by using inseticides are carried out, there are reports of control failures, which are probably due to insect resistance. Bioassay tests with topical application to study the status as well as resistance mechanism of house flies collected from the city of Jakarta (JKT), Bandung (BDG), Yogyakarta (YGY) and Surabaya (SBY) to pyrethroid (permethrin) and carbamate (propoxur) insecticides were carried out. The results show that, as compared to the most susceptible strain (YGY), we found that BDG strain is highly resistant to permethrin with resistance ratio (RR) of 133.7 fold. SBY strain was the most resistant to propoxur with RR of 38.36 fold, followed by Bandung strain with RR of 18.22 fold. The addition of piperonyl butoxide (PBO) to either permethrin or propoxur in most of the strains caused a decrease in LD50 values and increasing the synergist ratio (SR) suggesting that the detoxifying enzyme mixed function oxidase (MFO) played an important role in the development of resistance to permethrin and propoxur. Our study is the first report of M. domestica resistance to permethrin and propoxur in Indonesia.

Optimization and Characterization of Wood Vinegar Produced by Shorea laevis Ridl Wood Pyrolysis Hasan Ashari Oramahi; Tsuyoshi Yoshimura; Elvi Rusmiyanto; Kustiati Kustiati
Indonesian Journal of Chemistry Vol 20, No 4 (2020)
Publisher : Universitas Gadjah Mada

In this study, the Box-Behnken Design (BBD) was employed to investigate the effects of wood particle size, pyrolysis temperature, and pyrolysis time on the production of wood vinegar from Indonesia “bengkirai” wood (Shorea laevis Ridl). Characterization of wood vinegar was conducted by gas chromatography-mass spectrometry (GC-MS). Three variable designs consisting of wood particle size (2.00, 2.38, and 3.36 mm), pyrolysis temperature (350, 400, and 450 °C), and pyrolysis time (105, 120, and 135 min) were employed in a BBD response surface methodology (RSM-BBD). RSM-BBD results suggested that maximum wood vinegar yield would be obtained with a wood particle size of 3.85 mm, pyrolysis temperature of 400 °C, and pyrolysis time of 93 min. In addition, the mathematical model indicated the maximum wood vinegar yield would be 30.31%. The main compounds in wood vinegar were acetic acid, 1-hydroxy-2-propanone, furfural, 2,3-pentanedione, phenol, 2-methoxy phenol, 2-methoxy-4-methyl phenol, 2,6-dimethoxy phenol, and 1,2,4-trimethoxybenzene.

Penggunaan Asap Cair dari Tandan Kosong Kelapa Sawit untuk Pengendalian Jamur Phytophthora citrophthora secara In Vitro Hasan Oramahi; Elvi Rusmiyanto; Kustiati Kustiati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 38, No 1 (2021)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2021.38.1.1265

Efficacy of wood vinegar produced from empty fruit bunches (EFB) against Phytophthora citrophthora fungus was evaluated. The objective of the present work is to to evaluate antifungal properties of wood vinegar from EFB against P. citrophthora fungus. Three kinds of wood vinegar were used three temperatures i.e. 350, 400 and 450 °C. Efficacy of wood vinegar from EFB for antifungal is a factorial 3 by 4 in a completely randomized design; the first factor was pyrolysis temperature of wood vinegar from EFB (350, 400, and 450 °C) and the second factor was treatment of concentration of wood vinegar from EFB (0, 0.5, 1.0, and 1.5 %, v/v). The results showed that pyrolysis temperature effect of wood vinegar from EFB and concentration of wood vinegar were significantly different for inhibition of fungal growth. The highest inhibition was on wood vinegar for EFB with temperature pyrolysis of 450 °C and concentration of wood vinegar of 1% with average value of 100%.

Optimization of Liquid Smoke from Shorea pachyphylla using Response Surface Methodology and its Characterization Hasan Ashari Oramahi; Kustiati; Elvi Rusmiyanto Pancaning Wardoyo
Science and Technology Indonesia Vol. 7 No. 2 (2022): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

The present study aims to optimize the processing variables producing liquid smoke from mabang wood (Shorea pachyphylla) by using Response Surface Methodology (RSM). In this investigation, a design of experiment with different combinations of pyrolysis temperature and pyrolysis time on the liquid smoke yield from mabang wood was applied. The response of the optimal yield, temperature, and time of pyrolysis was predicted using a mathematical model. The optimal operating conditions for the process of yielding 31.31% liquid smoke were identified at the pyrolysis temperature of 440◦C and pyrolysis time of 124 minutes. The effect of pyrolysis temperature was more significant than the pyrolysis time (p<0.05). The liquid smoke samples were evaluated by a GC-MS. The main chemical compound of the liquid smoke were 1,2-ethanediol (19.26%), fluoromethane (6.69%), formic acid (4.96%), 2-propanone (4.17%), acetic acid (18.64%), acetol (4.80%), furfural (9.94%), 2,4-hexadecanoic acid (3.45%), and guaiacol (2.93%).

Co-Authors Ari Hepi Yanti Ari Widiyantoro Cut Novianti Rachmi, Cut Novianti Dasapta Erwin Irawan, Dasapta Erwin Defny Holidin Diah Wulandari Rousdy Elvi Rusmiyanto Pancaning Wardoyo, Suci Lestari, Mukarlina, Firman Saputra Flori, Florianus Florianus Flori Galih Kusumah, Galih Hasan Ashari Oramahi Hendy Irawan, Hendy Holyness Nurdin Singadimedja Ihsan Almuhardi Indra, Sutarto Kusuma Indri Kusharyanti Indrianasari, Melvy Intan Ahmad Irwan Lovadi Istiqomah Desi, Isti Junardi Junardi Juneman Abraham Keukeu Kaniawati Rosada Kukuh Hernowo Lia Resa Sinaga Linawati Linawati Linawati Linawati Moch Tanzil Multazam Mukarlina, Mukarlina Noni Yunizar Nova Hariani Nurhazman Abdul Aziz, Nurhazman Abdul Priyandono, Hendro Rafdinal Rafdinal Rahmawati, Rahmawati Resti Rahayu Ricco, Fredicus Rikhsan Kurniatuhadi, Rikhsan Rivaldy, Marsianus Marthin Riyandi Riyandi Riza Linda Saputra, Firman Septriono Hari Nugroho Silvi Susanti Siti Ifadatin Sri Yusmalinar Suprayoga, Rifqi Dwi Syarief Adiansyah Tedy Rismawan Trisnowati Budi Ambarningrum Tsuyoshi Yoshimura Turnip, Masnur Widadi Padmarsari Soetignya Yerslin Mantolu Yunizar, Noni Zulfa Zakiah

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