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Dewi, Fitriya Nur Annisa
Biomedical Laboratory, Center For Primate Animal Studies, Institute For Research And Community Service, IPB University, Bogor, Indonesia
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Journal : Jurnal Veteriner

 1 
Karakterisasi dan Diferensiasi Sel Punca Mesenkimal Asal Jaringan Adiposa Cokelat Monyet Ekor Panjang (Macaca fascicularis) Kartika Sari; Silmi Mariya; Irma H Suparto; Fitriya Nur Annisa Dewi; Permanawati Permanawati; Huda S Darusman; Dondin Sajuthi
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.448

Abstract

Mesenchymal stem cells (MSCs) from adipose tissue, known as multipotent stem cells, is a promising source of stem cells due to its abundance and it can be easily harvested, one of these cells is brown adipose tissue-derived cells which have gained new interest the field of degenerative disease. The aim of this study was to establish MSCs culture from brown adipose tissue (BAT) of Macaca fascicularis, particularly to characterize and evaluate their differentiation ability. MSCs were isolated from BAT obtained from biopsy of three adult male M. fascicularis on scapular area. MSCs were cultured and Polymerase Chain Reaction technique was used to confirm expression of markers for MSCs. The cells were further differentiated using growth media specific for osteocytes, chondrocytes and adipocytes. The results showed that BAT-derived MSCs had fibroblast-like morphology with spindle shape. Markers for MSCs (CD73, CD90, and CD105) were expressed. Importanty, the cells were able to differentiate into osteocytes, adipocytes and chondrocytes. These results showed that BAT of M. fascicularis can be a source for MSCs; these cells may be used further as in vitro model in the studies of regenerative medicine.
Co-Authors . Permanawati Agus Wijaya Anita Esfandiari Dedi Fardiaz Deni Noviana Deny Setyo Wibowo Diah Iskandriati Diah Iskandriati Diah Iskandriati Diah Iskandriati DIAH ISKANDRIATI Diah Iskandriati Diny Agustini Sandrasari Dondin Sajuthi Dwi Ramadhani Effendi, Siti Aisyah Rahmalia Eric Hayes Eric Hayes ESTHER ARIFIN Farida Laila Firman Alamsyah Fitria Senja Murtiningrum FITRY TAFZI Gregory K. Wilkerson Harrold Halilintar MR Subu Taopan Hendra Wijaya Hidekazu Shigematsu Hidekazu SHIGEMATSU Huda S Darusman Huda Shalahudin Darusman I Nengah Budiarsa I Nengah Budiarsa Iin Indriawati Iin Kurnia Hasan Basri Irma H Suparto Irma Herawati Suparto Izzatun Niswah Ajrina J. Mark Cline Joko Pamungkas Kartika Sari Kartjito, Nicolas Edward Christanto Lilis Nuraida MOSES AFFANDI Nancy Dewi Yuliana Nishigaki, Toshiaki Nuri Andarwulan NURI ANDARWULAN2 Pamungkas, Joko Permanawati Permanawati Permanawati Permanawati PUSPO EDI GIRIWONOB R.P Agus Lelana Ratni Ernita Retno Wulansari Safrida Safrida Sela S Mariya Sela S. Mariya Sela Septima Mariya Setyo Widodo Shigematsu, Hidekazu Silmi Mariya Silmi Mariya Silmi Mariya Silmi Mariya Silvia Arin Prabandari Slamet Budijanto Solinda, Gamelita Rizkawandi Sri Wahyuni Suryo Saputro Sus Derthi Widhyari Tatang Mitra Setia Teja Kisnanto Toshiaki Nishigaki Toshiaki NISHIGAKI Uus Saepuloh Uus Saepuloh UUS SAEPULOH Villiandra Villiandra Villiandra Villiandra Villiandra Villiandra Warsito P. Taruno Widanti, Berlian Wijaya, Hendra Yasmina A Pramastri Yasmina A. Paramastri Yasmina Arditi Pramastri YASMINA PARAMASTRI Yeni Kurniati Yeni Kurniati