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All Journal JPMS (Jurnal Pendidikan Matematika dan Sains) Jurnal Penelitian Fisika dan Aplikasinya (JPFA) Jurnal Matematika & Sains Jurnal Sumberdaya Alam dan Lingkungan Science and Technology Indonesia Emerging Science Journal ALCHEMY Jurnal Penelitian Kimia Indonesian Journal of Chemistry Jurnal Analis Kesehatan ALKIMIA : Jurnal Ilmu Kimia dan Terapan Jurnal Abdi Insani Sinar Sang Surya: Jurnal Pusat Pengabdian kepada Masyarakat Analit: Analytical And Environmental Chemistry

Sutopo Hadi

Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Universitas Lampung, Bandar Lampung, 35145, Indonesia

Author-ID : 272268

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Physics Social Sciences

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PEMANFAATAN SAMPAH PLASTIK MANJADI PAVING BLOK DI DESA HAJIMENA–NATAR,LAMPUNG SELATAN Yuli Ambarwati; Syaiful Bahri; Valennisa Qunifah; Lina Marlina; Sutopo Hadi; Aspita Laila; John Hendri
Jurnal Abdi Insani Vol 9 No 3 (2022): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v9i3.581

Hajimena Village is part of the Natar District which is directly adjacent to the Bandar Lampung City area, so that garbage disposal also enters from Bandar Lampung. The introduction and application of the 3R (Reduce, Reuse, Recycle) principle or the reduction, reuse and recycling of waste is one of the approaches in waste management. Inorganic waste can be converted into handicrafts that can be sold, especially plastic waste can be or processed into paving blocks. The Department of Chemistry Faculty of Mathematics and Natural Sciences University of Lampung, Community Service Team carried out community service activities aimed at increasing public awareness and participation in managing waste independently, reducing plastic waste and being a source of income for BUMDES. The activity of making paving blocks with variations of plastic waste, used oil, rice husks, and sand using simple technology and tools. The results of community service activities can be seen from the evaluation results in the form of measuring the achievement of specific instructional goals (ICT) from activities carried out using pre-test and post-test given to participants. The results show that there has been an increase in ICT for each participant after this service activity was carried out, it can be seen that each ICT has increased with an average increase of about 60.4%. This shows that public knowledge about plastic waste has increased significantly. So that the processing of plastic waste can be an opportunity to increase the income of the Hajimena village community.

Immobilization and Stabilization of Aspergillus Fumigatus α-Amylase by Adsorption on a Chitin Yandri Yandri; Ezra Rheinsky Tiarsa; Tati Suhartati; Bambang Irawan; Sutopo Hadi
Emerging Science Journal Vol 7, No 1 (2023): February
Publisher : Ital Publication

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28991/ESJ-2023-07-01-06

In this research, immobilization of A. fumigatus α-amylase on chitin was studied with the main purpose to improve the characteristics of the enzyme. A series of experiments were carried out to study stability improvement, thermodynamic parameters, include ki, ΔGi, and t½, and reusability of the immobilized enzyme. The experimental results indicate that significant thermal stability was achieved, as indicates by the ability of the enzyme to retain its relative activity above 39% after 80 min of incubation at 60oC. Thermodynamic parameters, include ki, ΔGi, and t½, indicate that the immobilized enzyme is more rigid, stable, and less flexible in the water, resulting in increased stability up to 1.5 times compared to that of the native enzyme. Furthermore, the immobilized enzyme was able to retain over 46% of its initial activity after six consecutive applications for starch hydrolysis, confirming the potential of chitin for the production of immobilized enzymes on an industrial scale. Doi: 10.28991/ESJ-2023-07-01-06 Full Text: PDF

Pelatihan Analisis Air Sumur Gali dan Sumur Bor di Kompleks Bataranila Hajimena Natar Lampung Selatan Tati Suhartati; Supriyanto Supriyanto; Yandri Yandri; Wasinton Simanjuntak; Sutopo Hadi
SINAR SANG SURYA Vol 7, No 1 (2023): Februari 2023
Publisher : UM Metro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24127/sss.v7i1.2544

ABSTRAK Telah dilaksanakan kegiatan pelatihan analisis air, khususnya analisis N-amonia pada sumur gali dan sumur bor di Kompleks Perumahan Bataranila Desa Hajimena Kecamatan Natar Lampung Selatan. Pelatihan ini bertujuan meningkatkan pemahaman tentang kesehatan melalui analisis sifat fisika-kimia air yang digunakan sehari-hari dan meningkatkan pengetahuan serta keterampilan penduduk tentang cara analisis kandungan amoniak dalam air secara mudah dan sederhana menggunakan metode kolorimetri. Materi yang disajikan pada kegiatan ini meliputi berbagai jenis air, persyaratan air minum, beberapa parameter air tercemar atau tidak, pengaruh berbagai kandungan kimia dalam air terhadap kesehatan, dan pelatihan secara singkat analisis N-amoniak dalam air. Metode kegiatan yang digunakan adalah metode ceramah dan tanya jawab, serta metode analisis kuantitatif kadar N-amoniak dalam air. Sedangkan rancangan evaluasi yang digunakan meliputi evaluasi awal, evaluasi proses, dan evaluasi akhir. Hasil evaluasi menunjukkan bahwa kegiatan ini telah berhasil meningkatkan pengetahuan masyarakat Bataranila tentang kualitas air secara umum dan cara cepat analisis kandungan N-amoniak dalam air.Kata kunci: Pelatihan Analisis Air, N-amoniak, Kolorimetri ABSTRACT The training of water analysis training activities has been performed, especially in the analysis of N-ammonia in dug and drilled wells at Perumahan Bataranila, Hajimena, Natar District, South Lampung. This training aims to improve the understanding of health through analysis of the physico-chemical properties of water used daily and to increase the knowledge and skills of residents on how to easily and simply analyze ammonia content in water using the colorimetric method. The material presented in this activity includes various types of water, drinking water requirements, several parameters of polluted or unpolluted water, the effect of various chemical constituents in water on health, and brief training on the analysis of N-ammonia in water. The activity method used is the lecture method and question and answer method, as well as the method of quantitative analysis of N-ammonia levels in water. While the evaluation design used includes initial evaluation, process evaluation, and final evaluation. The evaluation results show that this activity has succeeded in increasing the knowledge of the Bataranila community about water quality in general and how to quickly analyze the N-ammonia content in water.Keywords: Water Analysis Training, N-ammonia, Colorimetry

Studi Perbandingan Sintesis dan Karakterisasi Dua Senyawa Organotimah(IV) 3-Hidroksibenzoat Hadi, Sutopo; Afriyani, Hapin
ALKIMIA Vol 1 No 1 (2017): ALKIMIA
Publisher : SCIENCE AND TECHNOLOGY FACULTY OF UNIVERSITAS ISLAM NEGERI RADEN FATAH PALEMBANG

Senyawa organotimah(IV) karboksilat dan semua turunannya menunjukan aktivitas biologi sangat kuat dan dikenal memiliki aktivitas penghambatan yang tinggi bahkan pada konsentrasi yang sangat rendah sehingga golongan senyawa ini sangat menarik untuk terus dipelajari. Melanjutkan keberhasilan dalam sintesis senyawa organotimah(IV) karboksilat pada beberapa penelitian sebelumnya, dalam makalah ini kami laporkan hasil sintesis senyawa difeniltimah(IV) di-(3-hidroksibenzoat) dan trifeniltimah(IV) 3-hidroksibenzoat dari bahan awal senyawa difeniltimah(IV) oksida dan trifeniltimah(IV) hidroksida dengan ligan asam 3-hidroksibenzoat . Senyawa hasil sintesis dikarakterisasi dengan beberapa teknik spektroskopi dan mikroanalisis unsur. Hasil analisis menunjukan kedua senyawa hasil sintesis memiliki kemurnian yang baik dengan waktu refluks 4 jam dan nilai persen rendemen berturut-turut 92,66% dan 88,60%.

Cellulase Activity Bacillus sp. Which Was Grown on A Medium with Aluminum Salt and Magnetic Field Exposure Lailatul Farihah; Sumardi Sumardi; C. N. Ekowati; Sutopo Hadi
Jurnal Sumberdaya Alam dan Lingkungan Vol 10, No 1 (2023)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsal.2023.010.01.3

ABSTRAK Aluminium adalah jenis kofaktor logam terbaik. Pemaparan medan magnet 0,2mT pada garam aluminium mampu meningkatkan aktivitas enzim selulase pada bakteri Bacillus sp. Penelitian ini bertujuan untuk mencari jenis garam aluminium terbaik untuk meningkatkan aktivitas enzim selulase. Aktivtas enzim diuji dengan dua cara yaitu Uji Indeks Selulolitik dan Uji Aktivitas Enzim dengan spektrofotometer. Penelitian ini adalah penelitian deskriptif dengan tiga perlakuan yaitu kontrol, penambahan garam aluminium tanpa paparan medan magnet, dan penambahan garam aluminium yang dipaparkan medan magnet. Jenis garam aluminium yang digunakan adalah AlCl3, Al2(PO4)3, dan Al2(SO4)3. Konsentrasi garam aluminium yang digunakan adalah 0.01% dan pemaparan medan magnet 0.2 dilakukan selama 10 menit. Hasil penelitian ini membuktikan bahwa aluminium mampu menjadi kofaktor enzim yang efektif. Pemaparan medan magnet pada garam aluminium mampu meningkatkan aktivitas enzim. Jenis garam aluminium terbaik adalah AlCl3 yang dipaparkan medan magnet 0,2mT selama 10 menit. Perlakuan ini mampu meningkatkan aktivitas enzim selulase sebesar 0.69 U/mL. Kemudian Al2(PO4)3 mampu meningkatkan aktivitas enzim sebesar 0.41 U/mL dan Al2(SO4)3 meningkatkan aktivitas sebesar 0.39 U/mL. Kata kunci: aktivitas selulolitik, garam aluminium, indeks selulolitik, medan magnet ABSTRACT Aluminum is the best metal cofactor enzyme. Exposure to the 0,2mT magnetic field on Aluminium salt is said to be able to increase cellulolytic activity. The purpose of the research is to know if the best aluminum salt can increase cellulolytic activity. This can be tested by Cellulolytic Index Test and Enzyme Activity Test. This experiment is descriptive research with three treatments; control, the addition of aluminum salt without an exposed magnetic field, and added aluminum salt exposed to a magnetic field. Salt aluminum used are AlCl3, Al2(PO4)3, and Al2(SO4)3. The concentration was 0.01% and exposed to a magnetic field of 0.2mT. The final results tell that aluminum is an effective cofactor. Exposure to a magnetic field on aluminum salts showed an increase in enzyme activity. The best result is AlCl3 exposed to a 0.2mT magnetic field. This was able to increase the cellulolytic activity by 0.69U/mL. Then Al2(PO4)3 increased enzyme activity by 0.41U/mL and Al2(SO4)3 increased activity by 0.39U/mL. Keywords: cellulolytic activity, aluminum salt, cellulolytic index, magnetic field

Anticancer Activity Study of Modified Artocarpin Compound from Pudau Plant (Artocarpus kemando Miq.) Tati Suhartati; Khalimatus Sa’diah; Yandri Yandri; Sutopo Hadi
Emerging Science Journal Vol 7, No 3 (2023): June
Publisher : Ital Publication

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28991/ESJ-2023-07-03-05

This research is a continuation of the successful isolation of artocarpin from the root of Artocarpus kemando Miq reported in our previous study. In the previous study, the artocarpin was characterized with UV-Vis and FTIR techniques. In this follow-up investigation, the artocarpin was subjected to a transesterification reaction using acetic anhydride and pyridine as catalysts, and the product of the reaction was specified as compound 1. The compound 1 was further characterized with different techniques to gain more complete data and then tested for anticancer activity test against P-388 murine leukemia cells. Characterizations of the compound 1 using 1H-NMR and 13C-NMR techniques suggest that the modification reaction resulted in the conversion of the -OH groups at C2' and 4' at the artocarpin molecule to -OOCH3, and based on the MS analysis, the compound was proposed to have the molecular formula of C30H32O8. Another important feature of compound 1 that should be noted is the significant improvement in stability compared to the unmodified artocarpin. Anticancer activity tests against P-388 murine leukemia cells revealed that compound 1 has an IC50of 2.35 µg/mL, confirming that the compound is categorized as an active anticancer agent and suggesting that the compound has promising potential that deserves further investigations. Doi: 10.28991/ESJ-2023-07-03-05 Full Text: PDF

Penyuluhan penggunaan bahan pengawet sintetis dalam makanan bagi ibu-ibu PKK dan masyarakat di Desa Bumi Raharjo, Kecamatan Bumi Ratu Nuban, Lampung Tengah Sutopo Hadi; Tati Suhartati; Yandri Yandri
SINAR SANG SURYA Vol 7, No 2 (2023): Agustus 2023
Publisher : UM Metro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24127/sss.v7i2.2780

ABSTRAKÂ Bahan kimia, terutama pengawet, semakin banyak digunakan dalam makanan. Penggunaan bahan kimia dalam bahan makanan memiliki dampak yang signifikan terhadap kehidupan manusia, terutama jika bahan tambahan yang melanggar hukum digunakan, yang merupakan ancaman besar bagi kesehatan mereka. Potensi bahaya bahaya meningkat dengan banyaknya bahan tambahan yang tidak boleh digunakan dalam makanan. Masyarakat, khususnya ibu-ibu PKK, harus memiliki pemahaman yang komprehensif tentang zat aditif, risiko, dan tindakan pencegahan keamanan untuk mengatasi masalah ini. Salah satu strategi untuk menyebarluaskan pengetahuan tersebut adalah dengan memberikan penyuluhan tentang pengawet sintetis pada makanan bagi ibu-ibu PKK dan masyarakat setempat di Desa Bumi Raharjo, Kecamatan Bumi Ratu Nuban, Lampung Tengah. Pendekatan ceramah dan tanya jawab akan digunakan sebagai metode kegiatan, dan praktik pengenalan bahan pengawet makanan juga akan disertakan. Penilaian awal dan akhir digunakan dalam desain evaluasi. Menurut temuan kajian, program ini berhasil meningkatkan kesadaran masyarakat tentang pengawet makanan sintetis di Desa Bumi Raharjo, Kecamatan Bumi Ratu Nuban, Lampung Tengah.Â Kata kunci: bahan pengawet alami, bahan pengawet kimia, zat aditifÂ ABSTRACTChemicals, particularly preservatives, are being used more and more in food items. The use of chemicals in food items has a significant impact on people's life, especially when unlawful additions are used, which poses a major threat to their health. The potential of harm increases with the number of additives that are not allowed to be used in food. The community, especially PKK women, has to have comprehensive understanding of additives, risks, and safety precautions in order to solve this issue. One strategy to spread this knowledge is to provide counseling sessions on synthetic preservatives in food for PKK women and the local population in Bumi Raharjo Village, Bumi Ratu Nuban District, Central Lampung. The lecture and question-and-answer approach will be utilized as the activity method, and practice recognizing food preservatives will also be included. Initial and final assessments are used in the evaluation design. According to the assessment findings, the program was successful in raising community awareness about synthetic food preservatives in Bumi Raharjo Village, Bumi Ratu Nuban District, Central Lampung.Keywords: synthetic preservatives, chemical preservatives, additives

Penyuluhan penggunaan pewarna sintetis dalam makanan bagi ibu-ibu PKK dan masyarakat di Desa Wates, Kecamatan Bumi Ratu Nuban, Lampung Tengah Yandri Yandri; Sutopo Hadi; Tati Suhartati; Hardoko Insan Qudus; Suripto Dwi Yuwono
SINAR SANG SURYA Vol 8, No 1 (2024): FEBRUARI 2024
Publisher : UM Metro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24127/sss.v8i1.3205

ABSTRAK Saat ini, makanan dan minuman masih menggunakan pewarna, terutama yang terbuat dari bahan kimia sintetik. Warna produk dipengaruhi oleh penggunaan zat warna sintetik pada komponen makanan dan minuman sehingga membuat produk lebih menarik bagi konsumen. Namun penggunaan yang terus menerus akan berdampak buruk bagi kesehatan. Masyarakat, khususnya ibu-ibu PKK, harus mengetahui dengan baik tentang pewarna sintetis, risikonya, dan tindakan pencegahan keamanan untuk mengatasi masalah ini. Salah satu tindakan yang dapat dilakukan Lembaga Pengabdian Masyarakat Universitas Lampung untuk meningkatkan pemahaman ini adalah dengan melakukan penyuluhan kepada ibu-ibu PKK dan masyarakat setempat di Desa Wates, Kecamatan Bumi Ratu Nuban, Lampung Tengah, tentang risiko penggunaan pewarna sintetis pada makanan. Hasil evaluasi menunjukkan bahwa kegiatan ini berhasil meningkatkan pengetahuan peserta; dengan menggunakan metode bertingkat diperoleh rata-rata peningkatan pengetahuan peserta pelatihan sebesar 50,43%, dari kelompok rendah (3,76) ke kategori sedang (7,40). Kata kunci: pewarna makanan, pewarna sintetis, zat aditif ABSTRACTNowadays, food and beverages still include colours, particularly those made from synthetic chemicals. The color of the product is impacted by the use of synthetic dyes in food and beverage components, making the product more appealing to consumers. But continued usage will have a negative effect on health. The community, especially PKK women, has to be well-informed about synthetic dyes, their risks, and safety precautions in order to solve this issue. One action the University of Lampung Community Service Institute may do to raise this understanding is to counsel PKK women and the local community in Wates Village, Bumi Ratu Nuban District, Central Lampung, on the risks associated with the use of synthetic colors in food. The evaluation's findings demonstrate that this activity was successful in raising participants' knowledge levels; using the multilevel method, there was an average gain of 50.43% in trainees' knowledge, moving them from the low group (3.76) to the medium category (7.40).Keywords:, food coloring, synthetic dyes, additives

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24127/sss.v8i2.3300

Saat ini, makanan dan minuman masih menggunakan pewarna, terutama yang terbuat dari bahan kimia sintetik. Warna produk dipengaruhi oleh penggunaan zat warna sintetik pada komponen makanan dan minuman sehingga membuat produk lebih menarik bagi konsumen. Namun penggunaan yang terus menerus akan berdampak buruk bagi kesehatan. Masyarakat, khususnya ibu-ibu PKK, harus mengetahui dengan baik tentang pewarna sintetis, risikonya, dan tindakan pencegahan keamanan untuk mengatasi masalah ini. Salah satu tindakan yang dapat dilakukan Lembaga Pengabdian Masyarakat Universitas Lampung untuk meningkatkan pemahaman ini adalah dengan melakukan penyuluhan kepada ibu-ibu PKK dan masyarakat setempat di Desa Wates, Kecamatan Bumi Ratu Nuban, Lampung Tengah, tentang risiko penggunaan pewarna sintetis pada makanan. Hasil evaluasi menunjukkan bahwa kegiatan ini berhasil meningkatkan pengetahuan peserta; dengan menggunakan metode bertingkat diperoleh rata-rata peningkatan pengetahuan peserta pelatihan sebesar 50,43%, dari kelompok rendah (3,76) ke kategori sedang (7,40).

Activity of Mangrove-Derived Fusarium equiseti 20CB07RF Extract Against Clinical, Antibacterial-Resistant Pseudomonas aeruginosa Bahri, Syaiful; Setiawan, Wawan Abdullah; Setiawan, Fendi; Lutfiah, Rosyidatul; Juliasih, Ni Luh Gede Ratna; Ambarwati, Yuli; Ahmadi, Peni; Arai, Masayoshi; Hendri, John; Hadi, Sutopo; Setiawan, Andi
Science and Technology Indonesia Vol. 9 No. 3 (2024): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.3.594-604

Endophytic fungi originating from mangroves are potential sources of secondary metabolites with varying bioactivities. This research explores the bioactive metabolites produced by endophytes derived from mangrove plants. Endophytic fungi were collected from various parts of several mangrove plants (roots, stems, and leaves, as well as the surrounding mud). A total of 17 endophytics fungi were obtained. The isolates were derived from the leaves (1 isolate), stems (8 isolates), roots (5 isolates), and surrounding mud (3 isolates). A single fungal colony was cultured using solid-state fermentation for 14 days. The fermented fungal biomass was extracted using ethyl acetate (EtOAc) and evaluated for its antibacterial activity against clinical pathogenic bacteria. In the preliminary screening, the EtOAc extract of the CB07RF1 isolate exhibited notable growth-inhibitory effects against Pseudomonas aeruginosa. The isolate was verified by molecular identification using a study of the rDNA internal transcribed spacer (ITS) sequence, revealed that isolate CB07RF1 was very similar to Fusarium equiseti (99% similarity). Isolate 20CB07RF1, obtained by solid-state fermentation using a rice medium indicated as peptide compound group, and featured active components that exhibited potent growth inhibitory activity against Pseudomonas aeruginosa at a concentration of 12.5 mg/mL. This study demonstrates, for the first time, that Fusarium equiseti extracts grown in a rice medium contain antimicrobial compounds that can inhibit the growth of P. aeruginosa, an important clinical pathogen known for its antibacterial resistance. These findings accent mangrove endophytic fungi as important sources of bioactive compounds and will advance related research in the fields of biotechnology, pharmacology, and life sciences.

Co-Authors Afriyani, Hapin Ahmadi, Peni Ambarwati, Yuli Ambarwati, Yuli Andi Setiawan Andi Setiawan Arai, Masayoshi Aspita Laila Bambang Irawan Bambang Irawan C. N. Ekowati Dwi Asmi Ezra Rheinsky Tiarsa Hardoko Insan Qudus Hardoko Insan Qudus Hendri Ropingi Heri Satria Heri Satria Humairoh Ratu Ayu Indah Pratiwi Irvana, Raihan Irza Sukmana John Hendri John Hendri John Hendri Juliasih, Ni Luh Gede Ratna Junaidi Junaidi Karo , Pulung Karo Khalimatus Sa’diah Lailatul Farihah Lina Marlina Lutfiah, Rosyidatul Malik, Daffa Abdul Marjunus, Roniyus Mita Rilyanti Muhammad Rizki Nadila, Nurul NANIK SUWARSO Ni Luh Gede Ratna Juliasih NOPIANI NOPIANI Nova Tri Irianti Noviany Noviany Nurul Nadila Pulung Karo-Karo Qudus, Hardoko Insan Ridho Nahrowi Setiawan, Fendi Suharso Suharso Sumardi Sumardi Supri Yanto Suripto Dwi Yuwono Suripto Dwi Yuwono Suripto Dwi Yuwono Suripto Dwi Yuwono, Suripto Dwi Surtono, Arif Syaiful Bahri SYAIFUL BAHRI Tati Suhartati Tati Suhartati Tati Suhartati Tati Suhartati Tati Suhartati Tati Suhartati Trevor G Appleton Valennisa Qunifah Wasinton Simanjuntak Wasinton Simanjuntak WAWAN ABDULLAH SETIAWAN, WAWAN ABDULLAH YANDRI AS Yandri Yandri Yandri Yandri Yandri Yandri Yandri Yandri Yandri Yandri Yandri Yandri

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