Claim Missing Document
Check
Articles

Found 26 Documents
Search

Synthesis of Cellulose–Polylactic Acid Microcapsule as a Delivery Agent of Rifampicin Suripto Dwi Yuwono; Ridho Nahrowi; Andi Setiawan; Ni Luh Gede Ratna Juliasih; Irza Sukmana; Wasinton Simanjuntak; Sutopo Hadi
Science and Technology Indonesia Vol. 7 No. 3 (2022): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (997.285 KB) | DOI: 10.26554/sti.2022.7.3.263-268

Abstract

In medicinal field, delivery agent is a very important substance to improve the efficiency of drug used by improving the stability and preventing the degradation of drug during the medical treatment. Due to these important roles of the drug delivery agent, the search of effective agent is continuously in progress. In this respect, this current research was carried out to synthesize cellulose–polylactic acid (cellulose-PLA), as a potential delivery agent of rifampicin for the curing of tuberculosis. Cellulose was isolated from cassava bagasse, while PLA was obtained from commercial supplier. The two raw materials were used to synthesize cellulose–PLA in 3.5% HCl as solvent under magnetic stirring. The product obtained was then characterized by Fourier-Transform Infrared spectroscopy (FTIR), Particle-Size Analysis (PSA), and Scanning Electron Microscopy (SEM). The FT-IR result showed the presence of hydroxy (3446 to 3429 cm−1) and carbonyl (1757 to 1759 cm−1), confirming the formation new bond between cellulose and PLA. The PSA characterization displays a particle-sizes of PLA are in the range of 960–92780 nm, while cellulose–PLA are in the range of 100–17730 nm demonstrating that cellulose-PLA combined to form more compact structures. The results of SEM analysis indicate the distinct feature of cellulose-PLA, and combination of the features in the cellulose and PLA image. The results of the dissolution test carried out two different concentrations of rifampicin revealed that the optimum dissolution (8.42%) was achieved with cellulose–PLA of 0.3%, dissolution time of 12 h, and pH of 7.4.
PEMANFAATAN SAMPAH PLASTIK MANJADI PAVING BLOK DI DESA HAJIMENA–NATAR,LAMPUNG SELATAN Yuli Ambarwati; Syaiful Bahri; Valennisa Qunifah; Lina Marlina; Sutopo Hadi; Aspita Laila; John Hendri
Jurnal Abdi Insani Vol 9 No 3 (2022): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v9i3.581

Abstract

Hajimena Village is part of the Natar District which is directly adjacent to the Bandar Lampung City area, so that garbage disposal also enters from Bandar Lampung. The introduction and application of the 3R (Reduce, Reuse, Recycle) principle or the reduction, reuse and recycling of waste is one of the approaches in waste management. Inorganic waste can be converted into handicrafts that can be sold, especially plastic waste can be or processed into paving blocks. The Department of Chemistry Faculty of Mathematics and Natural Sciences University of Lampung, Community Service Team carried out community service activities aimed at increasing public awareness and participation in managing waste independently, reducing plastic waste and being a source of income for BUMDES. The activity of making paving blocks with variations of plastic waste, used oil, rice husks, and sand using simple technology and tools. The results of community service activities can be seen from the evaluation results in the form of measuring the achievement of specific instructional goals (ICT) from activities carried out using pre-test and post-test given to participants. The results show that there has been an increase in ICT for each participant after this service activity was carried out, it can be seen that each ICT has increased with an average increase of about 60.4%. This shows that public knowledge about plastic waste has increased significantly. So that the processing of plastic waste can be an opportunity to increase the income of the Hajimena village community.
Immobilization and Stabilization of Aspergillus Fumigatus α-Amylase by Adsorption on a Chitin Yandri Yandri; Ezra Rheinsky Tiarsa; Tati Suhartati; Bambang Irawan; Sutopo Hadi
Emerging Science Journal Vol 7, No 1 (2023): February
Publisher : Ital Publication

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28991/ESJ-2023-07-01-06

Abstract

In this research, immobilization of A. fumigatus α-amylase on chitin was studied with the main purpose to improve the characteristics of the enzyme. A series of experiments were carried out to study stability improvement, thermodynamic parameters, include ki, ΔGi, and t½, and reusability of the immobilized enzyme. The experimental results indicate that significant thermal stability was achieved, as indicates by the ability of the enzyme to retain its relative activity above 39% after 80 min of incubation at 60oC. Thermodynamic parameters, include ki, ΔGi, and t½, indicate that the immobilized enzyme is more rigid, stable, and less flexible in the water, resulting in increased stability up to 1.5 times compared to that of the native enzyme. Furthermore, the immobilized enzyme was able to retain over 46% of its initial activity after six consecutive applications for starch hydrolysis, confirming the potential of chitin for the production of immobilized enzymes on an industrial scale. Doi: 10.28991/ESJ-2023-07-01-06 Full Text: PDF
Pelatihan Analisis Air Sumur Gali dan Sumur Bor di Kompleks Bataranila Hajimena Natar Lampung Selatan Tati Suhartati; Supriyanto Supriyanto; Yandri Yandri; Wasinton Simanjuntak; Sutopo Hadi
SINAR SANG SURYA Vol 7, No 1 (2023): Februari 2023
Publisher : UM Metro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24127/sss.v7i1.2544

Abstract

ABSTRAK Telah dilaksanakan kegiatan pelatihan analisis air, khususnya  analisis N-amonia pada sumur gali dan sumur bor di Kompleks Perumahan Bataranila Desa Hajimena Kecamatan Natar Lampung Selatan. Pelatihan ini bertujuan meningkatkan pemahaman tentang kesehatan melalui analisis sifat fisika-kimia air yang digunakan sehari-hari dan meningkatkan pengetahuan serta keterampilan penduduk tentang cara analisis kandungan amoniak dalam air secara mudah dan sederhana menggunakan metode kolorimetri. Materi yang disajikan pada kegiatan ini meliputi berbagai jenis air, persyaratan air minum, beberapa parameter air tercemar atau tidak, pengaruh berbagai kandungan kimia dalam air terhadap kesehatan, dan pelatihan secara singkat analisis N-amoniak dalam air. Metode kegiatan yang digunakan adalah metode ceramah dan tanya jawab, serta metode analisis kuantitatif kadar N-amoniak dalam air.  Sedangkan rancangan evaluasi yang digunakan meliputi evaluasi awal, evaluasi proses, dan evaluasi akhir.  Hasil evaluasi menunjukkan bahwa kegiatan ini telah berhasil meningkatkan pengetahuan masyarakat Bataranila tentang kualitas air secara umum dan cara cepat analisis kandungan N-amoniak dalam air.Kata kunci: Pelatihan Analisis Air, N-amoniak, Kolorimetri ABSTRACT The training of water analysis training activities has been performed, especially in the analysis of N-ammonia in dug and drilled wells at Perumahan Bataranila, Hajimena, Natar District, South Lampung. This training aims to improve the understanding of health through analysis of the physico-chemical properties of water used daily and to increase the knowledge and skills of residents on how to easily and simply analyze ammonia content in water using the colorimetric method. The material presented in this activity includes various types of water, drinking water requirements, several parameters of polluted or unpolluted water, the effect of various chemical constituents in water on health, and brief training on the analysis of N-ammonia in water. The activity method used is the lecture method and question and answer method, as well as the method of quantitative analysis of N-ammonia levels in water. While the evaluation design used includes initial evaluation, process evaluation, and final evaluation. The evaluation results show that this activity has succeeded in increasing the knowledge of the Bataranila community about water quality in general and how to quickly analyze the N-ammonia content in water.Keywords: Water Analysis Training, N-ammonia, Colorimetry
Studi Perbandingan Sintesis dan Karakterisasi Dua Senyawa Organotimah(IV) 3-Hidroksibenzoat Hadi, Sutopo; Afriyani, Hapin
ALKIMIA Vol 1 No 1 (2017): ALKIMIA
Publisher : SCIENCE AND TECHNOLOGY FACULTY OF UNIVERSITAS ISLAM NEGERI RADEN FATAH PALEMBANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (521.115 KB) | DOI: 10.19109/alkimia.v1i1.1327

Abstract

Senyawa organotimah(IV) karboksilat dan semua turunannya menunjukan aktivitas biologi sangat kuat dan dikenal memiliki aktivitas penghambatan yang tinggi bahkan pada konsentrasi yang sangat rendah sehingga golongan senyawa ini sangat menarik untuk terus dipelajari. Melanjutkan keberhasilan dalam sintesis senyawa organotimah(IV) karboksilat pada beberapa penelitian sebelumnya, dalam makalah ini kami laporkan hasil sintesis senyawa difeniltimah(IV) di-(3-hidroksibenzoat) dan trifeniltimah(IV) 3-hidroksibenzoat dari bahan awal senyawa difeniltimah(IV) oksida dan trifeniltimah(IV) hidroksida dengan ligan asam 3-hidroksibenzoat . Senyawa hasil sintesis dikarakterisasi dengan beberapa teknik spektroskopi dan mikroanalisis unsur. Hasil analisis menunjukan kedua senyawa hasil sintesis memiliki kemurnian yang baik dengan waktu refluks 4 jam dan nilai persen rendemen berturut-turut 92,66% dan 88,60%.
Cellulase Activity Bacillus sp. Which Was Grown on A Medium with Aluminum Salt and Magnetic Field Exposure Lailatul Farihah; Sumardi Sumardi; C. N. Ekowati; Sutopo Hadi
Jurnal Sumberdaya Alam dan Lingkungan Vol 10, No 1 (2023)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsal.2023.010.01.3

Abstract

ABSTRAK Aluminium adalah jenis kofaktor logam terbaik. Pemaparan medan magnet 0,2mT pada garam aluminium mampu meningkatkan aktivitas enzim selulase pada bakteri Bacillus sp. Penelitian ini bertujuan untuk mencari jenis garam aluminium terbaik untuk meningkatkan aktivitas enzim selulase. Aktivtas enzim diuji dengan dua cara yaitu Uji Indeks Selulolitik dan Uji Aktivitas Enzim dengan spektrofotometer. Penelitian ini adalah penelitian deskriptif dengan tiga perlakuan yaitu kontrol, penambahan garam aluminium tanpa paparan medan magnet, dan penambahan garam aluminium yang dipaparkan medan magnet. Jenis garam aluminium yang digunakan adalah AlCl3, Al2(PO4)3, dan Al2(SO4)3. Konsentrasi garam aluminium yang digunakan adalah 0.01% dan pemaparan medan magnet 0.2 dilakukan selama 10 menit. Hasil penelitian ini membuktikan bahwa aluminium mampu menjadi kofaktor enzim yang efektif. Pemaparan medan magnet pada garam aluminium mampu meningkatkan aktivitas enzim. Jenis garam aluminium terbaik adalah AlCl3 yang dipaparkan medan magnet 0,2mT selama 10 menit. Perlakuan ini mampu meningkatkan aktivitas enzim selulase sebesar 0.69 U/mL. Kemudian Al2(PO4)3 mampu meningkatkan aktivitas enzim sebesar 0.41 U/mL dan Al2(SO4)3 meningkatkan aktivitas sebesar 0.39 U/mL. Kata kunci: aktivitas selulolitik, garam aluminium, indeks selulolitik, medan magnet ABSTRACT Aluminum is the best metal cofactor enzyme. Exposure to the 0,2mT magnetic field on Aluminium salt is said to be able to increase cellulolytic activity. The purpose of the research is to know if the best aluminum salt can increase cellulolytic activity. This can be tested by Cellulolytic Index Test and Enzyme Activity Test. This experiment is descriptive research with three treatments; control, the addition of aluminum salt without an exposed magnetic field, and added aluminum salt exposed to a magnetic field. Salt aluminum used are AlCl3, Al2(PO4)3, and Al2(SO4)3. The concentration was 0.01% and exposed to a magnetic field of 0.2mT. The final results tell that aluminum is an effective cofactor. Exposure to a magnetic field on aluminum salts showed an increase in enzyme activity. The best result is AlCl3 exposed to a 0.2mT magnetic field. This was able to increase the cellulolytic activity by 0.69U/mL. Then Al2(PO4)3 increased enzyme activity by 0.41U/mL and Al2(SO4)3 increased activity by 0.39U/mL. Keywords: cellulolytic activity, aluminum salt, cellulolytic index, magnetic field
Anticancer Activity Study of Modified Artocarpin Compound from Pudau Plant (Artocarpus kemando Miq.) Tati Suhartati; Khalimatus Sa’diah; Yandri Yandri; Sutopo Hadi
Emerging Science Journal Vol 7, No 3 (2023): June
Publisher : Ital Publication

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28991/ESJ-2023-07-03-05

Abstract

This research is a continuation of the successful isolation of artocarpin from the root of Artocarpus kemando Miq reported in our previous study. In the previous study, the artocarpin was characterized with UV-Vis and FTIR techniques. In this follow-up investigation, the artocarpin was subjected to a transesterification reaction using acetic anhydride and pyridine as catalysts, and the product of the reaction was specified as compound 1. The compound 1 was further characterized with different techniques to gain more complete data and then tested for anticancer activity test against P-388 murine leukemia cells. Characterizations of the compound 1 using 1H-NMR and 13C-NMR techniques suggest that the modification reaction resulted in the conversion of the -OH groups at C2' and 4' at the artocarpin molecule to -OOCH3, and based on the MS analysis, the compound was proposed to have the molecular formula of C30H32O8. Another important feature of compound 1 that should be noted is the significant improvement in stability compared to the unmodified artocarpin. Anticancer activity tests against P-388 murine leukemia cells revealed that compound 1 has an IC50of 2.35 µg/mL, confirming that the compound is categorized as an active anticancer agent and suggesting that the compound has promising potential that deserves further investigations. Doi: 10.28991/ESJ-2023-07-03-05 Full Text: PDF
PENINGKATAN KESTABILAN ENZIM α–AMILASE DENGAN PENAMBAHAN GLISEROL Yandri Yandri; Nurul Nadila; Tati Suhartati; Heri Satria; Sutopo Hadi
Analit: Analytical and Environmental Chemistry Vol 5, No 2 (2020): Analit: Analytical and Environmental Chemistry
Publisher : Universitas Lampung Jl. Prof. Dr. Sumatri Brojonegoro No.1 Bandar Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (439.329 KB) | DOI: 10.23960/aec.v5i2.2020.p143-154

Abstract

Enzim α-amilase adalah enzim yang mengkatalisis hidrolisis ikatan α-1,4 glikosidik polisakarida menghasilkan dekstrin, oligosakarida, maltosa, dan D-glukosa. Penelitian ini bertujuan meningkatkan kestabilan enzim α-amilase hasil pemurnian dari Aspergillus fumigatus dengan penambahan gliserol. Aktivitas enzim ditentukan dengan metode Fuwa dan Mandels, sedangkan kadar protein ditentukan dengan metode Lowry. Hasil penelitian menunjukkan enzim hasil pemurnian memiliki aktivitas spesifik sebesar 1,377 U/mg meningkat sebanyak 8,1 kali dibandingkan dengan ekstrak kasar enzim yang memiliki aktivitas spesifik sebesar 0,170 U/mg. Enzim hasil pemurnian memiliki pH optimum 5,5; suhu optimum 55 °C; waktu paruh 133,26 menit. Enzim setelah penambahan gliserol 0,5; 1 dan 1,5 M memiliki pH optimum dan suhu optimum yang sama, yaitu pH 5,5 dan suhu 55 °C. Enzim setelah penambahan gliserol:  0,5 M memiliki waktu paruh 157,5 menit: 1 M memiliki waktu paruh 182,36 menit, dan 1,5 M memiliki waktu paruh 223,54 menit. Penambahan gliserol pada enzim α-amilase hasil pemurnian dari A. fumigatus dapat meningkatkan kestabilan enzim sebanyak 1,2-1,7 kali dibandingkan dengan enzim hasil pemurnian yang ditunjukkan dengan  peningkatan waktu paruh.https://jurnal.fmipa.unila.ac.id/analit/article/view/2684/1868
Penyuluhan penggunaan bahan pengawet sintetis dalam makanan bagi ibu-ibu PKK dan masyarakat di Desa Bumi Raharjo, Kecamatan Bumi Ratu Nuban, Lampung Tengah Sutopo Hadi; Tati Suhartati; Yandri Yandri
SINAR SANG SURYA Vol 7, No 2 (2023): Agustus 2023
Publisher : UM Metro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24127/sss.v7i2.2780

Abstract

ABSTRAK Bahan kimia, terutama pengawet, semakin banyak digunakan dalam makanan. Penggunaan bahan kimia dalam bahan makanan memiliki dampak yang signifikan terhadap kehidupan manusia, terutama jika bahan tambahan yang melanggar hukum digunakan, yang merupakan ancaman besar bagi kesehatan mereka. Potensi bahaya bahaya meningkat dengan banyaknya bahan tambahan yang tidak boleh digunakan dalam makanan. Masyarakat, khususnya ibu-ibu PKK, harus memiliki pemahaman yang komprehensif tentang zat aditif, risiko, dan tindakan pencegahan keamanan untuk mengatasi masalah ini. Salah satu strategi untuk menyebarluaskan pengetahuan tersebut adalah dengan memberikan penyuluhan tentang pengawet sintetis pada makanan bagi ibu-ibu PKK dan masyarakat setempat di Desa Bumi Raharjo, Kecamatan Bumi Ratu Nuban, Lampung Tengah. Pendekatan ceramah dan tanya jawab akan digunakan sebagai metode kegiatan, dan praktik pengenalan bahan pengawet makanan juga akan disertakan. Penilaian awal dan akhir digunakan dalam desain evaluasi. Menurut temuan kajian, program ini berhasil meningkatkan kesadaran masyarakat tentang pengawet makanan sintetis di Desa Bumi Raharjo, Kecamatan Bumi Ratu Nuban, Lampung Tengah. Kata kunci: bahan pengawet alami, bahan pengawet kimia, zat aditif ABSTRACTChemicals, particularly preservatives, are being used more and more in food items. The use of chemicals in food items has a significant impact on people's life, especially when unlawful additions are used, which poses a major threat to their health. The potential of harm increases with the number of additives that are not allowed to be used in food. The community, especially PKK women, has to have comprehensive understanding of additives, risks, and safety precautions in order to solve this issue. One strategy to spread this knowledge is to provide counseling sessions on synthetic preservatives in food for PKK women and the local population in Bumi Raharjo Village, Bumi Ratu Nuban District, Central Lampung. The lecture and question-and-answer approach will be utilized as the activity method, and practice recognizing food preservatives will also be included. Initial and final assessments are used in the evaluation design. According to the assessment findings, the program was successful in raising community awareness about synthetic food preservatives in Bumi Raharjo Village, Bumi Ratu Nuban District, Central Lampung.Keywords: synthetic preservatives, chemical preservatives, additives
Penyuluhan penggunaan pewarna sintetis dalam makanan bagi ibu-ibu PKK dan masyarakat di Desa Wates, Kecamatan Bumi Ratu Nuban, Lampung Tengah Yandri Yandri; Sutopo Hadi; Tati Suhartati; Hardoko Insan Qudus; Suripto Dwi Yuwono
SINAR SANG SURYA Vol 8, No 1 (2024): FEBRUARI 2024
Publisher : UM Metro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24127/sss.v8i1.3205

Abstract

 ABSTRAK Saat ini, makanan dan minuman masih menggunakan pewarna, terutama yang terbuat dari bahan kimia sintetik. Warna produk dipengaruhi oleh penggunaan zat warna sintetik pada komponen makanan dan minuman sehingga membuat produk lebih menarik bagi konsumen. Namun penggunaan yang terus menerus akan berdampak buruk bagi kesehatan. Masyarakat, khususnya ibu-ibu PKK, harus mengetahui dengan baik tentang pewarna sintetis, risikonya, dan tindakan pencegahan keamanan untuk mengatasi masalah ini. Salah satu tindakan yang dapat dilakukan Lembaga Pengabdian Masyarakat Universitas Lampung untuk meningkatkan pemahaman ini adalah dengan melakukan penyuluhan kepada ibu-ibu PKK dan masyarakat setempat di Desa Wates, Kecamatan Bumi Ratu Nuban, Lampung Tengah, tentang risiko penggunaan pewarna sintetis pada makanan. Hasil evaluasi menunjukkan bahwa kegiatan ini berhasil meningkatkan pengetahuan peserta; dengan menggunakan metode bertingkat diperoleh rata-rata peningkatan pengetahuan peserta pelatihan sebesar 50,43%, dari kelompok rendah (3,76) ke kategori sedang (7,40).  Kata kunci: pewarna makanan, pewarna sintetis, zat aditif  ABSTRACTNowadays, food and beverages still include colours, particularly those made from synthetic chemicals. The color of the product is impacted by the use of synthetic dyes in food and beverage components, making the product more appealing to consumers. But continued usage will have a negative effect on health. The community, especially PKK women, has to be well-informed about synthetic dyes, their risks, and safety precautions in order to solve this issue. One action the University of Lampung Community Service Institute may do to raise this understanding is to counsel PKK women and the local community in Wates Village, Bumi Ratu Nuban District, Central Lampung, on the risks associated with the use of synthetic colors in food. The evaluation's findings demonstrate that this activity was successful in raising participants' knowledge levels; using the multilevel method, there was an average gain of 50.43% in trainees' knowledge, moving them from the low group (3.76) to the medium category (7.40).Keywords:, food coloring, synthetic dyes, additives