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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Buletin Peternakan Jurnal Veteriner BERITA BIOLOGI Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Entomologi Indonesia Jurnal Kedokteran Hewan
Surachmi Setiyaningsih
Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jln. Agatis, Kampus IPB Dramaga, Bogor, Jawa Barat 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Veterinary Other

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Journal : Jurnal Veteriner

 1 
Metode Direct Polymerase Chain Reaction untuk Melacak Campylobacter sp. pada Daging Ayam (DIRECT POLYMERASE CHAIN REACTION METHOD FOR DETECTION CAMPYLOBACTER SP. OF POULTRY MEAT) Andriani .; Mirnawati Sudarwanto; Surachmi Setiyaningsih; Harsi Dewantari Kusumaningrum
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.705 KB)

Abstract

Campylobacter sp. is the most commonly reported as agent of foodborne zoonosis causing acutegastroenteritis in humans. Poultry meat is considered as a major source of C. jejuni infection in human.The conventional methods for detecting foodborne bacteria is time-consuming which rely on the of thebacteria in culture media, followed by biochemical identification. In this study polymerase chain reaction(PCR) technique was used for rapid identification of the pathogenic Campylobacter sp. The samples usedwere 298 chicken carcass with sold in supermarkets and traditional markets, and were carried out inaccordance the isolation protocol ISO/ DIS 10272-1994. Identification was performed using biochemicalAPI Campy. The direct PCR (DPCR) assay with two sets of primers was employed for isolation andidentification of C. jejuni and C. coli. The result of the isolation and identification both by conventional orPCR methods showed that chicken carcasses both from supermarket and traditional market werecontaminated with C. jejuni and or C. coli. Prevalence of Campylobacter sp. contamination in chicken meatwas higher by DPCR (62.6%) than by conventional (19.8%), indicating that DPCR technique was moresensitive than conventional method with detection limit for C. jejuni was103 cfu/ml.
Pengembangan Enzyme-Linked Immunosorbent Assay Paratuberkulosis dengan Antigen Protoplasmik Mycobacterium avium Subspecies Paratuberculosis Isolat Lapang (DEVELOPMENT OF PARATUBERCULOSIS ENZYME-LINKED IMMUNO-SORBENT ASSAY WITH PROTOPLASMIC ANTIGEN OF MYCO Rahmat Setya Adji; I Wayan Teguh Wawan; Denny Widaya Lukman; Surachmi Setiyaningsih
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Enzyme-linked immunosorbent assay (ELISA) is a serological test method most widely used for thediagnosis of paratuberculosis, because it has a better sensitivity compared to other serological test.Protoplasmic antigen (PPA) or cellular extract is still the main choice for the diagnosis of paratuberkulosisdevelopment. The aim of research was to use the PPA Mycobacterium avium subspeciesparatuberculosis(MAP) field isolates for the development of paratuberculosis ELISA (ELISA PPA-L). As many as 322cattle sera (300 negative and 22 positive) were tested using this method and compared with IDEXXcommercial kit. The sensitivity and specificity of ELISA PPA-L test results were 68.18% and 97.0%,whereas for the IDEXX kit were 63.64% and 97.33%respectively. ELISA PPA-L had higher sensitivity andlower specificity compared to the IDEXX commercial kit. ELISA test using protoplasmic antigen of MAPfield isolates has good ability for paratuberculosis serological test and can be used for screening test of thedisease in Indonesia.
STUDI HISTOPATOLOGI PADA LIMPA DAN BURSA FABRICIOUS AYAM NEWCASTLE DISEASE DARI KASUS LAPANG HISTOPATHOLOGY (STUDIES ON SPLEEN AND BURSA OF FABRICIUS OF NEWCASTLE DISEASE CHICKHENS FROM FIELD CASE) Etriwati Etriwati; Ekowati Handharyani; Surachmi Setiyaningsih
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.822 KB) | DOI: 10.19087/jveteriner.2017.18.4.510

Abstract

Newcastle Disease is a highly contagious and very virulent avian disease in all of poultry. The aims of this study were to determine the lesions and distribution of Newcastle disease virus on spleen and bursa of Fabricous of Newcastle disease chickhens from field cases. The sample used in this study were the spleen and bursa Fabricious organ of ten cases of suspect Newcastle disease. Haematoxylin and eosin staining examination was performed determine of cell morphology and distribution of the virus by immunohistochemical staining. The distribution of the lesions and the degree of reaction were determined by the category immunopositive mild, moderate and severe. Gross lesion on spleen, including swollen, fragile and necrotic multifocal. Histopathological lesions showed hyperemia/congestion and depletion of lymphoid cells. Immunopositive reaction found in lymphoid cells of red pulp and lymphoid cells in lymphoid follicles. Bursa of Fabricious showed atrophy and hyperemia/congestion with histopatological lesions hemorrhages, lymphoid cells in lymphoid follicles undergo necrosis and depletion of lymphoid follicles. Immunopositif reaction was observed in lymphoid cells in the mantle zone of lymphoid follicles. Newcastle disease from field cases cause damage to the spleen and bursa of Fabricius with degrees lesions and virus distribution in the organs are severe.
The Kejadian Pertama Rabbit Haemorrhagic Disease Berdasarkan Studi Seroprevalensi di Provinsi Jawa Barat, Indonesia Retno Setyaningsih; I Wayan Teguh Wibawan; Surachmi Setiyaningsih; Ekowati Handharyani; Sri Murtini; Ahmad Biharidin
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.3.409

Abstract

Rabbit Haemmorhagic Disease (RHD) is one of viral diseases in rabbits that is still exotic in Indonesia. The RHD disease is caused by the Rabbit Haemorrhagic Disease Virus (RHDV) which is a calicivirus of the genus Lagovirus, Caliciviridae family. The high genetic variation of the RHDV and the rapid spread have the potential to disrupt rabbit farm (rabbittery) production and trade activities, especially rabbit exports, which require clarity on the status and situation of RHD disease in rabbits in Indonesia. A case of suspected RHD in Indonesia was first detected in the Philippines where rabbits exported from Indonesia were detected to be seropositive to RHD which resulted in rabbit export activities to the country was stopped. There is no data on the presence of RHD disease in rabbits in Indonesia, so the seroprevalence study is useful to provide preliminary information on the presence of this disease in Indonesia. This study was conducted on 163 rabbits samples raised in the Lembang area, Bandung, West Java, which is known as the largest rabbit farming center in Indonesia. Rabbit samples were taken at rabbit farms spread across seven villages namely Lembang, Pagerwangi, Cikahuripan, Cikole, Sukajaya, Gudangkahuripan and Jambudipa. The rabbits serum samples obtained were tested using the Indirect Enzym Linked Immunosorbant Assay (ELISA) method to determine the titer of antibodies against RHD. Based on the results of the analysis, it is known that 120 out of 163 rabbit serum samples showed positive antibody titers against RHD. The presence of antibody titers in rabbits on such farms can be preliminary information to be able to carry out further studies.
Prevalensi Virus Demam Babi Afrika pada Produk Daging Babi yang Diuji di Laboratorium Balai Besar Uji Standar Karantina Pertanian Seruni Agistiana; , I Wayan Teguh Wibawan; Surachmi Setiyaningsih; Ni Luh Putu Ika Mayasari; Harimurti Nuradji; Sriyanto Sriyanto; Risma Juniarti Paulina Silitonga
Jurnal Veteriner Vol 24 No 3 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.3.264

Abstract

Demam babi afrika atau African swine fever (ASF) adalah penyakit viral lintas batas yang sangat menular pada babi domestik dan babi liar yang disebabkan oleh virus ASF (ASFV). Virus ini memiliki daya tahan hidup dan stabilitas yang tinggi di lingkungan, mampu bertahan lama pada benda mati seperti peralatan dan pakaian yang tercemar atau pada produk daging dari babi yang terinfeksi ASFV. Penelitian ini bertujuan mendeteksi ASFV pada produk daging babi dan olahannya di Indonesia. Sejak tahun 2019, Balai Besar Uji Standar Karantina Pertanian (BBUSKP) yang merupakan salah satu laboratorium rujukan milik pemerintah di bawah naungan Badan Karantina Pertanian (Barantan), memiliki tugas untuk melakukan pengujian deteksi ASFV salah satunya adalah menguji sampel produk daging babi. Prevalensi dihitung berdasarkan data sekunder hasil pengujian qualitative Polymerase Chain Reaction (qPCR) dalam deteksi ASFV pada produk daging babi yang dilakukan di laboratorium BBUSKP, Jakarta Timur tahun 2020-2022. Terdeteksinya ASFV pada produk daging babi sebanyak 8.2% di tahun 2020, 24.8% di tahun 2021 dan 11.6% pada tahun 2022 memperkuat bahwa produk daging babi berpotensi menjadi media pembawa ASF di Indonesia.
Co-Authors . Andriani . Darniati Aditya Primawidyawan Agustin Indrawati Ahmad Biharidin Andriani . Andriani a Andriani a Andriani Andriani Andriani Andriani Angela Mariana Lusiastuti Ari Rumekso Ayunina, Yasmine Qurrota Bambang Pontjo Priosoeryanto Darniati D, Darniati Daud AK, M Denny Widaya Lukman Dilas Pradana Ekowati Handharyani Emilia A Emilia A, Emilia Erin Kurnianingtyas Etriwati E Eva Harlina Fadilla, Zahara Ferry Ardiawan Harimurti Nuradji Harsi D. Kusumaningrum Herwin Pisestyani I Wayan Teguh Wawan I wayan Teguh Wibawan Idwan Sudirman Idwan Sudirman Kuntum Khoirani Lanniari, Nafrina M Daud AK Mirnawati Soedarwanto Mirnawati Soedarwanto Mirnawati Sudarwanto MIRNAWATI SUDARWANTO Mirnawati Sudarwanto Mirnawati Sudarwanto Ni Luh Putu Ika Mayasari Nur Khusni Hidayanto Okabayashi, Tamaki Okti Nadia Poetri Putu Angga Wiradana Putu Eka Sudaryatma Rachmi Ridho Rahmat Setya Adji Retno Damayanti Soejoedono Retno Setyaningsih Retno Setyaningsih Retno Wulansari Risma Juniarti Paulina Silitonga Rona Choiruz Zaujat Rostantinata, Riski Seruni Agistiana Seruni Agistiana sri murtini . Sriyanto Sriyanto Subangkit, Mawar Trioso Purnawarman Upik Kesumawati Hadi Yasmine Qurrota Ayunina