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All Journal Jurnal Sain Veteriner Indonesian Journal of Biotechnology Jurnal Medik Veteriner Jurnal AME (Aplikasi Mekanika dan Energi): Jurnal Ilmiah Teknik Mesin Livestock and Animal Research Indonesian Journal of Advanced Research (IJAR) Jurnal Kedokteran Hewan Jurnal Sosialita: Jurnal Kajian Sosial dan Pendidikan
Aris Haryanto
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Education Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Social Sciences Veterinary

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Evaluation of synthetic‐gene recombinant LipL32 antigen for IgM ELISA detection of Leptospira infection Widiastuti, Dyah; Paramita, Dewi Kartikawati; Murwanti, Retno; Kusrini, Ina; Wijayanti, Nastiti; Haryanto, Aris
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.111102

Abstract

Leptospirosis presents with nonspecific clinical features and requires time‐consuming laboratory tests for gold standard diagnosis. This study aims to design and characterize the recombinant LipL32 from synthetic gene and assess its performance as an antigen for detecting leptospirosis. The antigen was developed by cloning the LipL32 gene conserved portion of Leptospira interrogans serovar Icterohaemorrhagiae strain Langkawi. The immunoinformatic was used to characterize the developed rLipL32. Western blot results using anti‐histidine revealed a band of rLipL32 protein at ~40 kDa. Subsequently, it was used to examine the IgM antibody on human sera by using ELISA. The IgM‐LipL32 ELISA was evaluated using 67‐positive and 25‐negative sera and compared with a commercial ELISA. With a cut‐off value of 0.8, it showed 85.7% sensitivity, 83.3% specificity, a 48% positive prediction value (PPV), and 97% negative prediction value (NPV), indicating modest performance compared to existing commercial kits. The rLipL32 is a potential antigen for detecting IgM using ELISA; however, for use in low incidence areas, a confirmation test is crucial.
Evaluation of primers targeting chromo helicase DNA-binding gene (CHD) for molecular sexing identification in four bird families Fitriana, Fauziah; Resita, Riza; Disastra, Yuda; Setyorini, Dian Ritma; Haryanto, Aris; Aziz, Fatkhanuddin
Livestock and Animal Research Vol 21, No 1 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i1.66998

Abstract

Objective: Sex determination of birds is crucial role in breeding and conservation purposes. Genomic approaches by using basis of bird sex polymorphism are accepted to generate an accurate sexing procedure. The PCR method is commonly used and known to provide accurate test results in determining the sex of birds, but its successes is strongly influenced by the compatibility of the primers used with the DNA template. This study aimed to determine the potential use of CHD1LF/CHD1LR targeting CHD gene fragment in 4 species from Phasianidae, Psittacidae, Estrildidae, and Passeridae families.Method: Blood samples (n= 30) from representatives of species in 4 families above were collected for DNA isolation, then PCR test was performed using CHD1LF/CHD1LR primers. The PCR results obtained were compared with positive controls, necropsy examination and information from bird sellers.Result: The results showed that the primer CHD1LF/CHD1LR could be used in Phasianidae,Psittacidae, Estrildidae, and Passeridae families. The PCR product was confirmed to be same as well as the positive control and necropsy results. It is demonstrated that male birds showed a single band (474 bp), while double bands (474 and 319 bp) were observed in female birds. Different accuracies were observed during this study between molecular approach and manual sexing by bird seller i.e:sparrows (46%) and finches (50%), while the accuracy of parakeets was 75%, and quails 80%.Conclusions: Primer CHD1LF/CHD1LR can be used to determine the sex of birds from the familiesPhasianidae, Psittacidae, Estrildidae, and Passeridae. Our study showed that molecular-based method is valuable and able to reduce error rate to 100% in bird sexing procedures.  
Co-Authors Andrian, Krisna Noli Charles Rangga Tabbu Dewi Kartikawati Paramita Dini Wahyu Yudianingtyas Disastra, Yuda Duhita Andinita Dyah Widiastuti Fatkhanuddin Aziz, Fatkhanuddin Fitriana, Fauziah Hera Nirwati Hevi Wihadmadyatami Ina Kusrini Kajang, Elphan Augusta Karnati, Srikanth Kayanaveda, Yohanna Medania Purwaningrum Michael Kann Muhammad Isa Nastiti Wijayanti Nenny Sri Mulyani Novindasari, Balqis Bahiya Milan Nurrahmi, Isti Ananda Nurul Ilmi, Nurul Purnomo Hadi Putra, I Nengah Putra, Rizky Dwiandra Resita, Riza Retno Murwanti Rini Widayanti salamah salamah Sawangmake, Chenphop Setya Permana Sutisna Setyorini, Dian Ritma Siahaan, Timbul Sri Handayani Irianingsih Sutaryo1 S, Sutaryo1 Sutoyo, Edi Teuku Zahrial Helmi Teuku Zahrial Helmy Titis Widowati, Titis Tri Wibawa Wayan Tunas Artama Widagdo Sri Nugroho