Hasriadi Mat Akin
Jurusan Proteksi Tanaman, Fakultas Pertanian Universitas Lampung, Bandar Lampung

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RESPON BEBERAPA GENOTIPE KEDELAI TERHADAP INFEKSI CPMMV (COWPEA MILD MOTTLE VIRUS) Hasriadi Mat Akin .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 3 No. 2 (2003): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (40.516 KB) | DOI: 10.23960/j.hptt.2340-42

Abstract

Response of various soybean genotipes against cowpea mild mottle virus (CPMMV) infection. The green house experiment was conducted to investigate the effect of CPMMV infection on growth and yield of various soybean varieties. Treatments were arranged in completely randomized block design in split plot experiment. Mainplots were virus-inoculated and uninoculated plants; subplots were eight soybean varieties: Wilis, Orba, MLG 2521, Taichung, B 3570, Dieng, Tidar, and Pangrango. The results of this experiment showed that CPMMV infection decreased vegetative growth and yield of soybean plant. The decrease of dry weigth of soybean plant proved that CPMMV infection inhibited the vegetative growth. Taichung variety susceptible to CPMMV was indicated by significant reduction of the growth and yield. B3570 line tolerance to CPMMV infection was proved by lower reduction of the yield.
PENGARUH INFEKSI TMV (TOBACCO MOSAIC VIRUS) TERHADAP PERTUMBUHAN VEGETATIF DAN GENERATIF BEBERAPA VARIETAS CABAI MERAH (CAPSICUM ANNUUM L.) Hasriadi Mat Akin dan Muhammad Nurdin .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 3 No. 1 (2003): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (45.292 KB) | DOI: 10.23960/j.hptt.1310-12

Abstract

Influence of tobacco mosaic virus infection to vegetative and generative growth of various hot pepper varieties (Capsicum annuum L.). This experiment was conducted to investigate the effect of TMV infection on vegetative and generative growth of various hot pepper varieties. Treatments were arranged in completely randomized design in split plot experiment with four replications. Mainplots were virus-inoculated and uninoculated plants; subplots were three hot pepper varieties: Cimerti, HP-Typhoon, and HP-Tornado. The results of the experiment showed that TMV infection caused decrease vegetative and generative growth. The decrease of vegetative and generative growth indicated by the reduction of leaf width, plant height, and yield. HP-Tornado and Cimerti varieties showed susceptible reaction proved by significant reduction of the growth and yield; HP-Typhoon was tolerance reaction to TMV infection indicated by significant reduction of the growth and lowest reduction of the yield.
Pola Segregasi Sifat Ketahanan Terhadap Soybean Stunt Virus dan Keragaman Genetik FAMILI F2:3 HASIL PERSILANGAN VARIETAS ORBA DAN GALUR B3570 Hasriadi Mat Akin, Emi Lidya Astri, dan Maimun Barmawi .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 9 No. 1 (2009): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (92.942 KB) | DOI: 10.23960/j.hptt.1973-77

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Segregation pattern of the Soybean Stunt Virus resistant character and genetic diversity of F2:3 families derived from crosses between Orba and B3570. Soybean stunt disease caused by SSV (Soybean Stunt Virus) is the most destructive soybean disease in Indonesia. This research was conducted from October 2005 to June 2006 at experiment station of Lampung University. The aims of this research were to evaluate the segregation of resistant characters and total genetic diversity of eight populations of F2:3 families. Experiment was arranged in a randomized complete block design with three replications. The resistance was evaluated based on the score of disease severity. The results showed that the resistant characters segregate 1:2:1 to susceptible, moderately resistant, and resistant, respectively based on the segregation pattern. The resistant character was controlled by single gene and the action of the gene is noncompletely dominant gene. Eight populations of F2:3 families have high diversities on the yield and yield components.
RESPON BEBERAPA VARIETAS KEDELAI (GLYCINE MAX (L.) MERRIL) TERHADAP INFEKSI PEANUT STRIPE POTYVIRUS Hasriadi Mat Akin dan Muhammad Nurdin .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 2 No. 2 (2002): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.398 KB) | DOI: 10.23960/j.hptt.2247-50

Abstract

Response of various soybean varieties [Glycine max (L.) Merril] againts peanut stripe potyvirus infection. This experiment was conducted to investigate the effect of PStV infection on soybean growth of various soybean varieties. Treatments were arranged in completely randomized design in split plot experiment with four replications. Main plots were virus-inoculated and uninoculated plants; subplots were eight soybean varieties (Wilis, Orba, MLG2521, Amerikana, Yellowbean, B3570, Slamet and Taichung). The results showed that infection decreased soybean growth indicated by reduction dry weight of soybean plant and reduction of soybean yield. Slamet variety compared to other varieties showed susceptible reaction indicated by the shortest incubation period (9,75 days), significant reduction of dry weigh of soybean plant, and the highest reduction of soybean yield (23,6%). Thaicung variety showed resistant reaction indicated by longest incubation period (14,3 days) and insignificant reduction of soybean growth and yield.
PENGGUNAAN PELACAK NONRADIOAKTIF (Digoxigenin-DNA Probe) UNTUK MENDETEKSI PEANUT STRIPE VIRUS Hasriadi Mat Akin
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 1 No. 2 (2001): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.994 KB) | DOI: 10.23960/j.hptt.2175-79

Abstract

The use of nonradioactive probe (Digoxigenin-DNA) for detection of peanut stripe virus. The objective of this experiment was to develop the nonradioactive-labeled probe to detect peanut stripe virus (PStV) in peanut leaves and seeds. Digoxigenin labeled cDNA (dig-DNA probe) was synthesized from recombinant plasmid (pHS1.23) using polymerase chain reaction (PCR). The probe containing 1.195 bp (base pair) corresponding to 3' termini, included part of NIb (nuclear inclusion body) gene, coat protein gene, and 3' untranslated region of PStV genome was used to detect the existence of PStV in peanut leaves and seeds of infected peanut plants.
PENGKLONAN DAN PERUNUTAN NUKLEOTIDA GEN SELUBUNG PROTEIN DAN 3’UTR (untranslated region) PEANUT STRIPE VIRUS Hasriadi Mat Akin
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 1 No. 1 (2001): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.755 KB) | DOI: 10.23960/j.hptt.111-6

Abstract

Cloning and sequencing of coat protein gene and 3’UTR (untranslated region) of peanut stripe virus. The cDNA of 3' terminal of peanut stripe virus genomic RNA was cloned and sequenced. The cDNA was ligated with plasmid vector pGEM-T Easy and transformed to competent cells of Escherichia coli. The 3' terminal of PstV genomic RNA contained 1195 nucleotides (nts). The region included the nucleotide sequences of NIb (nuclear inclusion body) (129 nts), CP gene (coat protein gene) (861 nts), and 3'UTR (untranslated region) (205 nts). The nucleotide sequence of a CP gene contained one long uninterrupted open reading frame (ORF) without a start codon, which ended a UAG stop codon. The 287 amino acid residues of PStV coat protein were predicted from the CP gene. The amino acid was analyzed for the presence of consensus polyprotein cleavage site for maturation of potyvirus polyprotein. A putative cleavage site was found at position 43 (Q/S) following the Valine (V) residue at -4 position. This isolate of PstV can be expected to be aphid transmissible because the coat protein contained a DAG triplet at position 53-55.
Pola SegregasiKarakter KetahananTanaman Kedelai(Glycine max [L]. Merrill)Terhadap Infeksi Soybean Mosaic Virus Populasi F2 Keturunan Taichung X Tanggamus Nidya Wanda; Maimun Barmawi; Hasriadi Mat Akin; Nyimas Sa’diyah
Jurnal Penelitian Pertanian Terapan Vol 15 No 1 (2015)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (480.316 KB) | DOI: 10.25181/jppt.v15i1.112

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This study aims to determine the pattern of segregation character soybean plant resistance to infection SMV and other agronomic characters as well as many genes that play a role in the inheritance of these traits in the F2 populations from crosses Taichung x Tanggamus follow of Mendel ratio or a modification of expectations and knowing the number of crosses. This research was conducted at the Laboratory LapanganTerpadu Faculty of Agriculture, University of Lampung from September 2013 until January 2014, and then continued in the laboratory observations Seed and Plant Breeding, University of Lampung. Propagation of virus carried in Kampung Baru, Bandar Lampung. This research uses experimental design without repetition with a single treatment design nested structured data is analyzed using the chi-square test for the suitability of the normal distribution and chi-squared test to test the of Mendel ratio and modifications . The results of this study indicate the character frequency distribution of plant height, grain weight per plant soybeans F2 generation from crosses Taichung x Tanggamus normal spread, while the frequency distribution for character flowering age, harvesting, healthy seed number per plant, number of productive branches, number of pods per plants and severity of disease spread is not normal. Estimates of the number of genes that control the character of harvesting is controlled by two genes are recessive epistasis duplicate the ratio 9: 7, days to flowering follow the ratio 1: 2: 1 which is controlled by a single gene that reacts not perfect, healthy character of the number of seeds per plant followed the ratio 3: 1 and for the total character of pods per plant, number of productive branches as well as the severity of the disease follows the ratio of 13: 3. the character dikendalikanolehdua genes dominant-recessive epistasis react. There are 21 numbers belonging expectations resistant to SMV. Keywords: Soybean, segregation, resistance characteristics, and SMV.
UJI EFEKTIVITAS TEPUNG UMBI TEKI (Cyperus rotundus L.) DALAM MENGENDALIKAN PENYAKIT ANTRAKNOSA PADA TANAMAN CABAI DILAPANG Denny Marini Sihite; Muhammad Nurdin; Suskandini Ratih Dirmawati; Hasriadi Mat Akin
Jurnal Agrotek Tropika Vol 8, No 1 (2020): Jurnal Agrotek Tropika Vol 8, Januari 2020
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1007.588 KB) | DOI: 10.23960/jat.v8i1.3670

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Penelitian ini bertujuan untuk mengetahui konsentrasi tepung umbi teki (Cyperus rotundus L.) yang efektif dalam mengendalikan penyakit antraknosa pada tanaman cabai. Penelitian ini dilaksanakan di Laboratorium Ilmu Penyakit Tanaman dan Laboratorium Lapang Terpadu Fakultas Pertanian, Universitas Lampung dimulai sejak bulan Agustus sampai November 2017. Perlakuan dalam penelitian ini disusun dalam Rancangan Acak Kelompok (RAK) dengan 4 ulangan.  Perlakuan terdiri dari kontrol (P0), fungisida propineb (P1), tepung umbi teki konsentrasi 5% (P2), tepung umbi teki konsentrasi 15% (P3), dan tepung umbi teki konsentrasi 25% (P4). Data yang diperoleh selanjutnya dianalisis dengan menggunakan sidik ragam dan kemudian dilanjutkan dengan uji beda nyata terkecil (BNT) pada taraf nyata 5% . Parameter yang diamati adalah keparahan penyakit, tinggi tanaman cabai, jumlah buah cabai, dan bobot buah cabai. Hasil penelitian menunjukkan bahwa tepung teki konsentrasi 5%, 15%, dan 25% efektif dalam menekan keparahan penyakit antraknosa pada tanaman cabai di lapang. Tepung teki dengan konsentrasi 5%,15%, dan 25%  memiliki keefektifan yang sebanding dengan fungisida propineb dalam menekan keparahan penyakit antraknosa pada tanaman cabai.
PENGARUH Trichoderma sp. SEBAGAI AGEN PENINGKATAN KETAHANAN TANAMAN PADI TERHADAP PENYAKIT HAWAR DAUN Geraldo Sandy; Suskandini Ratih; Radix Suharjo; Hasriadi Mat Akin
Jurnal Agrotek Tropika Vol 7, No 3 (2019): JAT September 2019
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.939 KB) | DOI: 10.23960/jat.v7i3.3546

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Rice is one commodity that have an important value for Indonesian people food. But the production of rice plant decreased by attacking of insect and bacterial. The bacterialleaf blight caused by Xanthomonas oryzaepv.oryzae. To avoid the bacterial leaf blight could used Trichoderma sp. suspension to induced resistant in rice plant. This research was conducted at the Laboratory of Plant Disease and Greenhouse of Lampung University. This study was conducted from June 2016 to August 2016.The experiment was conducted in a complete randomized design arranged with two factors, namely varieties of rice and kind isolate of Trichoderma sp.. The results of this study showed that the application of Trichoderma sp. reducing the severity of bacterial leaf blight diseaseand the kind isolate of Trichoderma sp. can increase the length rootand height of rice plant.
KETAHANAN BEBERAPA GENOTIPE SORGUM (Sorghum bicolor [L]Moench) TERHADAP PENYAKIT ANTRAKNOSA (Colletotrichum graminicola) PADA DUA SISTEM POLA TANAM BERBEDA Agnes Ratnasari; Efri Efri; Muhammad Syamsoel Hadi; Hasriadi Mat Akin
Jurnal Agrotek Tropika Vol 7, No 2 (2019): JAT Mei 2019
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v7i2.3258

Abstract

Tujuan penelitian ini yaitu mengetahui ketahanan 15 genotipe sorgum yang ditanam pada dua sistem tanam berbeda yaitu monokultur dan tumpangsari. Penelitian ini dilaksanakan pada bulan April 2017- Februari 2018 di Desa Sukanegara, Kecamatan Tanjung Bintang, Kabupaten Lampung Selatan, Lampung dan di Laboratorium Hama dan Penyakit Tanaman Fakultas Pertanian Universitas Lampung. Perlakuan disusunmenggunakan rancangan acak kekompok dalam Split Plot Design dengan faktor utama adalah sistem pola tanam (tumpangsari, monokultur), dan anak petak adalah 15 genotipe sorgum (Numbu, Samurai 1, GH3, UPCA, GH4, P/I WHP, GH6, Super 2, GH13, P/F 51-93-C, Super 1, GH5, Mandau, GH7 dan TalagaBodas). Monokultur sorgum ditanam pada jarak 80 cm x 20 cm. Tumpangsari sorgum ubikayu dilakukan dengan cara menanam sorgum di antara tanaman ubikayusehingga jarak tanam sorgum tetap 80 cm x 20 cm, sedangkan jarak tanam ubikayu 80 cm x 60 cm, baik sorgum maupun ubikayu ditanam secara bersamaan. Hasil penelitian menunjukkan bahwa sistem tanam tumpangsari lebih efektif untuk menekan intensitas penyakit antraknosa. Pada penelitian ini intensitas penyakit antraknosa terhadap 15 genotipe sorgum yang diamati dikelompokan menjadi 3 kategori yaitu tinggi, sedang dan rendah. Genotipe Numbu, GH3, Talaga Bodas, Super 1, dan Mandau adalah genotipe dengan intensitas penyaki terendah dibandingkan genotipe Samurai 1, UPCA, GH4, P/I WHP, GH13, P/F 5-193-C, GH5, GH6 dan GH7 . Genotipe Samurai 1, UPCA, GH4, P/I WHP, GH13, P/F 5-193-C, GH5, GH6 dan GH7 adalah genotipe yang intensitas penyakitnya lebih rendah dibandingkan genotipe Super 2. Dan genotipe Super 2 adalah genotipe dengan intnsitas penyakit antraknosa tertinggi.