Hasriadi Mat Akin
Jurusan Proteksi Tanaman, Fakultas Pertanian Universitas Lampung, Bandar Lampung

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INVENTARISASI JAMUR PADA BIJI KOPI ROBUSTA (Coffea canephora Pierre) OLAHAN NATURAL KLON TUGU SARY DARI DUA DESA DI KABUPATEN TANGGAMUS Muhammad Nurdin; Hasriadi Mat Akin; Ermawati Ermawati; Riski Mardiana
Jurnal Agrotek Tropika Vol 12, No 3 (2024): JURNAL AGROTEK TROPIKA VOL 12, AGUSTUS 2024
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v12i3.9464

Abstract

Inventarisasi dan identifikasi merupakan kegiatan penting dalam pengelolaan penyakit tanaman karena dengan diketahuinya identitas jamur yang terdapat pada biji kopi akan dapat ditentukan strategi pencegahan lebih awal dan tepat waktu untuk mencegah terjadinya penyebaran jamur. Penelitian ini bertujuan untuk mengetahui jamur-jamur yang berasosiasi dengan biji kopi dan persentase infeksinya pada biji kopi olahan natural klon Tugu Sary dari dua desa di Kabupaten Tanggamus. Penelitian ini dilaksanakan di Laboratorium Ilmu Penyakit Tanaman Fakultas Pertanian Universitas Lampung dari bulan Maret 2021 sampai dengan Mei 2021. Penelitian ini dilaksanakan dengan pengujian metode agar (agar plate), media yang digunakan yaitu potato sucrose agar (PSA), Terdapat dua sampel jenis biji kopi asal pengambilan sampel, yaitu desa Sedayu ditumbuhkan pada delapan cawan media PSA (empat cawan dengan perendaman NaOCl 2% dan empat cawan tanpa perendaman NaOCl) dan desa Datar Lebuay ditumbuhkan pada delapan cawan media PSA (empat cawan dengan perendaman NaOCl 2% dan empat cawan tanpa perendaman NaOCl) sehingga terdapat 16 cawan petri. Isolat yang sudah didapatkan kemudian diidentifikasi pada hari ke-7. Pengamatan pada penelitian ini dilakukan secara makroskopis dan mikroskopis. Pengamatan makroskopis dilakukan untuk membedakan jamur yang tumbuh berdasarkan warna, bentuk dan ukuran koloninya. Sedangkan pengamatan mikroskopis dilakukan untuk membedakan jamur yang tumbuh berdasarkan struktur tubuh jamur. Hasil penelitian menunjukkan bahwa pada biji kopi olahan natural klon Tugu Sary dari Desa Sedayu dan Desa Datar Lebuay terdapat empat spesies jamur, yaitu Aspergillus flavus, Aspergillus niger, Aspergillus westerdijkiae dan Rhizoctonia sp. Jamur Aspergillus niger dan Rhizoctonia sp. merupakan jamur dengan persentase serangan tertinggi pada biji kopi asal Desa Sedayu dan Desa Datar Lebuay. Persentase serangan jamur pada biji kopi asal desa Sedayu adalah Rhizoctonia sp. 38,75%, Aspergillus niger 25%, Aspergillus flavus 0% dan Aspergillus westerdijkiae 0%. Sedangkan persentase serangan jamur pada biji kopi asal desa Datar Lebuay adalah Rhizoctonia sp. 58,75%, Aspergillus niger 32,5%, Aspergillus flavus 1,25% dan Aspergillus westerdijkiae 1,25%.
INVENTARISASI PENYAKIT TANAMAN BUAH NAGA (Hylocereus polyrhizus) DI KABUPATEN WAY KANAN Jefry Fernando Purba; Cipta Ginting; Hasriadi Mat Akin; Joko Prasetyo; Ivayani Ivayani
Jurnal Agrotek Tropika Vol 10, No 3 (2022): JURNAL AGROTEK TROPIKA VOL 10, AGUSTUS 2022
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v10i3.6195

Abstract

Salah satu permasalahan dalam budidaya tanaman buah naga ialah adanya serangan hama dan patogen tanaman, namun informasi mengenai penyakit penting tanaman buah naga di Lampung masih kurang. Tujuan penelitian ialah untuk mengetahui penyakit-penyakit yang terdapat pada tanaman buah naga di daerah penanaman penting di Kabupaten Way Kanan. Penelitian ini dilaksanakan di Laboratorium Ilmu Penyakit Tumbuhan dan Laboratorium Bioteknologi Fakultas Pertanian Universitas Lampung. Pengambilan sampel tanaman sakit dilakukan di Desa Bumiharjo, Kecamatan Buah Bahuga, Kabupaten Way Kanan, Provinsi Lampung. Metode yang digunakan pada penelitian ini adalah observasi, analisis dan deskriptif yaitu dengan melakukan wawancara kepada petani, pengamatan dan pengambilan sampel, pembuatan media PSA (potato sucrose agar) dan media YPA (Yeast Peptone Agar), isolasi dan pemurnian jamur, identifikasi patogen, serta uji patogenesitas. Berdasarkan hasil penelitian dapat disimpulkan bahwa terdapat beberapa penyakit pada pertanaman buah naga di Kabupaten Way Kanan, yaitu kudis (Pestalotiopsis sp.), layu fusarium (Fusarium sp.), antraknosa (Collectrotricum sp.), kanker batang (Neoscytalidium dimidiatum), karat merah (Cephaleuros sp.), busuk lunak (Xanthomonas), dan busuk pangkal sulur (Sclerotium rolfsii).
Molecular characterization and effectiveness cross-protection of weak strains against super-infection malignant strains Cucumber mosaic virus (CMV) on cayenne pepper (Capsicum annuum L.) in Lampung, Indonesia Mahfut, Mahfut; Sari, Mai; Akin, Hasriadi Mat; Wahyuningsih, Sri; Irawan, Bambang
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12563-75

Abstract

Cayenne pepper (Capsicum annuum L.) is a horticultural crop of significant economic importance. One of the major threats to its cultivation is the Cucumber mosaic virus (CMV), which can cause severe yield losses and crop failures. This study aimed to isolate weak CMV strains from natural populations of cayenne pepper, characterize their molecular properties, and evaluate the effectiveness of cross-protection conferred by weak strains against superinfection by virulent CMV strains. The research was conducted in three stages: virus isolation, molecular characterization, and evaluation of cross-protection effectiveness. Data analysis was performed using the MEGA v.11.0.11 software and analysis of variance (ANOVA). The findings identified the C1 isolate as a highly virulent CMV strain. Molecular analysis of naturally infected samples confirmed the presence of CMV through the amplification of specific DNA bands. Inoculation tests revealed distinct differences between weak and malignant strains, particularly in symptom severity and molecular characteristics, such as genetic distance. Furthermore, the weak strains demonstrated a protective effect, significantly reducing the infection rate of virulent CMV strains. This was evidenced by variations in symptom expression, disease severity, plant resistance, chlorophyll levels, carbohydrate content, and peroxidase enzyme activity. These findings highlight the potential application of weak CMV strains in developing disease management strategies for cayenne pepper cultivation.
Antifungal evaluation of turmeric rhizome extract against Colletotrichum capsici, the causal agent of anthracnose on red-chili peppers (Capsicum annuum L.) Akin, Hasriadi Mat; Anggraini, Desma; Wibowo, Lestari; Prasetyo, Joko; Suharjo, Radix
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 1 (2024): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12475-81

Abstract

This study was conducted to evaluate the antifungal activity of the ethanolic extract of turmeric rhizome against Colletotrichum capsici Syd. and its efficacy in reducing anthracnose disease development on red chili peppers. The in vitro experiment aimed to determine the effect of the extract on inhibiting C. capsici growth in potato sucrose agar (PSA) medium incorporating the turmeric rhizome extract. The variables examined were mycelial growth, fungal sporulation, and spore germination. The in vivo experiment aimed to evaluate the effectiveness of the extract in reducing disease development on red chili peppers. The treatments were conducted using a completely randomized design with five concentration treatments and three replicates. The variables examined were radial mycelial growth zone diameter, fungal sporulation, spore germination, and disease intensity. Data were analyzed by analysis of variance, and the least significant difference was used for comparing means between treatments. The results of these experiments showed that ethanolic turmeric rhizome extract exhibited antifungal activity against C. capsici both in vitro and in vivo conditions. Turmeric rhizome extracts with concentrations of 1%, 2%, 3%, and 4% significantly inhibited mycelial growth, and even at a 4% concentration, C. capsici fungal colonies did not grow. Evaluation of the effect of the extract on fungal sporulation showed that inhibition occurred at a concentration of 3%, while inhibition of spore germination occurred at concentrations of 1%, 2%, and 3%. Application of turmeric extract at concentrations of 1%, 2%, 3%, and 4% effectively reduced the intensity of anthracnose on red chili. Read correction >
IDENTIFIKASI JAMUR YANG TERBAWA BIJI EMPAT KLON KAKAO (Theobroma cacao L.) PADA PENANGANAN ASALAN DAN PENANGANAN TERKENDALI Nurdin, Muhammad; Evizal, Rusdi; Akin, Hasriadi Mat; Safitri, Annisa
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.7875

Abstract

Identification is an important activity in the management of plant diseases because by knowing the identity of the fungus found in cocoa beans, a strategy can be determined as a first step before controlling the fungus to prevent the spread of the fungus. This research aims to determine the fungi associated with the MCC 01, MCC 02, Sulawesi 1, and THR clones on uncontrol handling and controlled handling cocoa beans and to determine whether uncontrol handling and controlled handling reduce inoculum sources to suppress fungi growth and development. This research was conducted at the Laboratory of Plant Diseases, Faculty of Agriculture, University of Lampung from January 2023 to March 2023. This research was carried out by testing the agar method (agar plate), the media used was potato sucrose agar (PSA). There were four samples of cocoa bean clones, namely MCC 01, MCC 02, Sulawesi 1, and THR from Kuta Dalom Village, Pesawaran Regency, which were grown on 128 plates of PSA media (64 plates using 2% NaOCl and 64 plates without NaOCl) for each clone. Cocoa beans contained 32 cups each uncontrol handling and controlled handling. So there are 128 cups of PSA media. Isolates that have been obtained are then identified 7 days after isolation. Observations in this study were carried out macroscopically and microscopically. Macroscopic observations were carried out to differentiate growing fungi based on the color, shape and size of the colonies. Meanwhile, microscopic observations were made to differentiate growing fungus based on the structure of the fungi body. The results showed that from the uncontrol handling and the controlled handling, three fungi were found, namely Aspergillus flavus, Aspergillus niger, and Rhizoctonia sp. The fungi Aspergillus flavus and Aspergillus niger show that controlled handling can reduce the source of inoculum compared to uncontrol handling. In contrast to the fungi Aspergillus flavus and Aspergillus niger, the fungi Rhizoctonia sp. shows that controlled handling cannot reduce the source of inoculum. Key words:  Aspergillus, bean, cocoa, Rhizoctonia, handling
Pendampingan Gapoktan Jaya Makmur dalam Pengelolaan Penyakit Bercak Cincin pada Tanaman Pepaya di Desa Bumi Jaya, Lampung Selatan Helina, Selvi; Akin, Hasriadi Mat; Solikhin, Solikhin; Maryono, Tri; Yusticia, Stenia Ruski
Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol. 3 No. 2 (2024): Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol 3 No 2, September
Publisher : Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpfp.v3i2.9443

Abstract

Gapoktan Jaya Makmur merupakan salah satu Gapoktan di Kecamatan Candipuro yang menjadikan tanaman pepaya sebagai salah satu komoditi unggulan di Kabupaten Lampung Selatan. Penyakit bercak cincin yang disebabkan oleh virus menjadi faktor pembatas dalam kegiatan budidaya tanaman pepaya di Desa Bumijaya. Minimnya pengetahuan petani dalam mengenali penyakit bercak cincin pada tanaman pepaya dan pengelolaannya di lapangan menjadi permasalahan utama yang belum bisa diatasi oleh kelompok tani di desa Bumijaya. Metode yang digunakan adalah pendampingan melalui penyuluhan penyakit bercak pepaya dan pengelolaan penyakit dengan prinsip pengendalian hama terpadu (PHT) serta pelatihan pembuatan agens hayati di lapangan. Hasil kegiatan pengabdian kepada masyarakat yang telah dilaksanakan dapat disimpulkan: 1) Kegiatan pengabdian kepada masyarakat diikuti oleh anggota dan pengurus Gapoktan Jaya Makmur, Desa Bumijaya, Lampung Selatan, POPT Kecamatan Candipuro, Penyuluh Pertanian Desa Bumijaya; 2) Peserta sangat antusias mengikuti kegiatan yang ditunjukan oleh keaktifannya dalam menyimak dan merespon penyampaian materi serta aktif bertanya dan memberi tanggapan terhadap setiap permasalahan yang dibahas, 3) Terjadi peningkatan penguasaan pengetahuan tentang Pengembangan Trichoderma sebagai Upaya Pengelolaan Penyakit bercak cincin pada tanaman pepaya yang Ditularkan oleh kutu putih (Aphids) di Candipuro, Lampung Selatan yang signifikan yang ditunjukkan oleh hasil evalusai setelah diberi penyuluhan lebih yaitu mayoritas peserta memiliki pengetahuan yang baik hingga sangat baik (96%) dan tidak ada peserta yang memiliki nilai dengan kriteria kurang. Dengan demikian pendampingan Gapoktan Jaya Makmur dalam kegiatan pengabdian ini sangat bermanfaat bagi kelompok tani dalam mengelola Penyakit Bercak Cincin pada tanaman pepaya di Desa Bumijaya, Lampung Selatan.
Efficacy of phosphonic acid applied by spraying and seed treatment at various concentrations for controlling downy mildew disease in maize Ginting, Cipta; Pramesti, Elisa Amelia; Maryono, Tri; Sudarsono, Hamim; Susanto, Herry; Akin, Hasriadi Mat
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225328-336

Abstract

Downy mildew, caused by Peronosclerospora spp., is one of the major problems in maize cultivation. Several studies have reported the development of resistance in Peronosclerospora spp. to metalaxyl, which had previously been an effective control method for the disease. Other research suggests that the efficacy of phosphonic acid in controlling downy mildew in maize is inconsistent. The objective of this study was to evaluate the efficacy of seed treatment with phosphonic acid in controlling downy mildew in Bisi 18 maize. The treatments were arranged in a randomized block design with six replications. The observed variables included the incidence and severity of the disease, the area under the disease progress curve (AUDPC), maize yield, and the content of tannin and hydroquinone compounds in maize leaves. The results showed that both phosphonic acid applied by spraying and seed treatment significantly reduced the incidence and severity of downy mildew (P < 0.05). However, the concentrations of phosphonic acid used in seed treatment had no significant effect on the disease during 1–7 weeks after inoculation. Furthermore, all phosphonic acid treatments tended to increase the content of tannin and hydroquinone compounds in maize leaves, which should be confirm quantitatively.
Begomovirus: Potensi ancaman pertanian hortikultura Lampung Helina, Selvi; Akin, Hasriadi Mat; Dirmawati, Suskandini Ratih; Fitriana, Yuyun; Maryono, Tri; Suharjo, Radix; Ginting, Cipta
Jurnal Proteksi Agrikultura Vol. 2 No. 2 (2025): NOVEMBER, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.2242-55

Abstract

Begomoviruses are a group of plant viruses belonging to the Geminiviridae family, persistently transmitted by the whitefly Bemisia tabaci, and pose a serious threat to horticultural crops in Lampung Province, Indonesia. These viruses infection symptoms such as mosaic, chlorosis, leaf curling, and stunted growth, with yield losses reaching up to 90%. The spread is exacerbated by intensive cultivation practices, the use of uncertified seeds, and tropical climates that favor high vector populations. This study explores the biological characteristics of Begomoviruses, their transmission mechanisms via vectors, and their high genetic variability due to frequent mutation and recombination. Several begomovirus species have been identified infecting key horticultural crops in Lampung, including Ageratum yellow vein virus (AYVV), Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV), and Pepper yellow leaf curl Indonesia virus (PepYLCIV). In addition to vector transmission, some begomovirus species are also known to be seed-transmitted, expanding their epidemic potential. Integrated management strategies discussed in this article include the use of resistant varieties, biological and agronomic control of vectors, and the development of innovative approaches such as mild strain cross-protection. This review emphasizes the need for continuous monitoring and science-based sustainable strategies to mitigate the impact of Begomoviruses on food security and horticultural productivity in tropical regions such as Lampung.