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All Journal Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Jurnal Manajemen Bisnis Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Biotechnology Majalah Obat Tradisional STRATEGIC INDONESIAN JOURNAL OF PHARMACY JURNAL MANAJEMEN DAN PELAYANAN FARMASI (Journal of Management and Pharmacy Practice) Majalah Farmaseutik YUME : Journal of Management Majalah Kesehatan Pharmamedika Jurnal Minfo Polgan (JMP) Indonesian Journal of Digital Business Jurnal Ilmiah Manajemen dan Bisnis (JIMBI) Journal of Artificial Intelligence and Digital Business Journal of Information Systems Management and Digital Business Journal of Management and Innovation Entrepreneurship (JMIE) Jurnal Kefarmasian Indonesia Indonesian Journal of Pharmacology and Therapy
Adam Hermawan
Departemen Kimia Farmasi, Fakultas Farmasi, Universitas Gadjah Mada, Sekip Utara II, Yogyakarta 55281, Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Journal : INDONESIAN JOURNAL OF PHARMACY

 1 
Hesperidin increase cytotoxic effect of doxorubicin in MCF-7 cells Hermawan, Adam; Meiyanto, Edy; Susidarti, Ratna Asmah
Indonesian Journal of Pharmacy Vol 21 No 1, 2010
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.522 KB) | DOI: 10.14499/indonesianjpharm0iss0pp8-17

Abstract

Hesperidin,  a  flavonoid,  shows  strong  cytotoxic  effect  in  several  cancer cell  lines.  The  aim  of  this  research  was  to  investigate  cytotoxic  activities  of hesperidin  alone  and  in  combination  with  doxorubicin.  Cell  viability  assay  of hesperidin,  doxorubicin,  and  combination  treatments  were  carried  out  by  using MTT  assay.  Apoptosis  assay  was  done  using  double  staining  method  using Ethidium  Bromide-Acridine  Orange.  Hesperidin  did  not  show  cytotoxic  effect but doxorubicin showed cytotoxic effect with IC50467 nM. Hesperidin (5, 50 and 100  µM)  increased  cytotoxic  effect  of  doxorubicin  compared  with  doxorubicin alone.  The  strongest  cytotoxic  activity  was  showed  by  the  combination  of  200 nM  doxorubicin  and  100  µM  hesperidin.  Combination  treatment  of  doxorubicin 200  nM  and  hesperidin  100  µM  induced  apoptosis  in  MCF-7 cells.  Hesperidin  is potentially  to  be  developed  as  co-chemotherapeutic  agent  for  breast  cancer, while molecular mechanism need to be explored.Key words: Hesperidin, doxorubicin, synergism, MCF-7, apoptosis 
Curcumin Analogs Induce Apoptosis and G2/M Arrest In 4T1 Murine Triple-Negative Breast Cancer Cells Retno Murwanti; Azmi Rahmadani; Ritmaleni Ritmaleni; Adam Hermawan; Bambang Sulistiyo Ari Sudarmanto
Indonesian Journal of Pharmacy Vol 31 No 1, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss1pp11

Abstract

Chemotherapy is the first-line treatment for triple-negative breast cancer (TNBC), yet toxicity and resistance effects have been the current problems. Curcumin,a natural compound, has been reported to exert anti-proliferative effects on various cancer cells, including breast carcinoma cells. However, the β-diketone moiety influences the stability of curcumin. Curcumin analogs, pentagamavunon-0 (PGV-0), and pentagamavunon-1 (PGV-1) were synthesized to improve the stability and activity of curcumin by modified the β-diketone moiety into mono-ketone pentanone. In this study, we evaluated the cytotoxicity, inhibition of cell cycle progression, and induction of apoptosis of curcumin and its analogs (PGV-0 and PGV-1) in murine triple-negative breast cancer 4T1 cell line. The cytotoxic evaluation was done by MTT assay, while apoptosis induction and cell cycle evaluation was performed by annexin V staining and detected by flow cytometry. Curcumin and its analogs, PGV-0, and  PGV-1, significantly inhibit the viability of 4T1 breast cancer cells with an IC50 value of 34.34µg/mL, 13.76µg/mL and 38.21μg/mL, respectively. Apoptosis analysis with a dose of 10µg/mL and 15µg/mL in 4T1 breast cancer cells showed that curcumin and its analogs effectively induce apoptotic in a dose-dependent manner. In cell cycle analysis using a dose of 15µg/mL, curcumin inhibited the cell cycle progression in the S phase, whereas PGV-0 and PGV-1 inhibited the cell cycle in the G2/M phase. It could be concluded that curcumin analogs, PGV-0 and PGV-1, have higher potential to be developed as anti-cancer agents by inducing cell cycle arrest and apoptosis in triple-negative breast cancer.
Hesperidin increase cytotoxic effect of doxorubicin in MCF-7 cells Adam Hermawan
Indonesian Journal of Pharmacy Vol 21 No 1, 2010
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3762.688 KB) | DOI: 10.14499/indonesianjpharm0iss0pp8-16

Abstract

Hesperidin, a flavonoid, shows strong cytotoxic effect in several cancer cell lines. The aim of this research was to investigate cytotoxic activities of hesperidin alone and in combination with doxorubicin. Cell viability assay of hesperidin, doxorubicin, and combination treatments were carried out by using MTT assay. Apoptosis assay was done using double staining method using Ethidium Bromide-Acridine Orange. Hesperidin did not show cytotoxic effect          but doxorubicin showed cytotoxic effect with IC50 467 nM. Hesperidin (5, 50 and  100 µM) increased cytotoxic effect of doxorubicin compared with doxorubicin alone. The strongest cytotoxic activity was showed by the combination of 200 nM doxorubicin and 100 µM hesperidin. Combination treatment of doxorubicin 200 nM and hesperidin 100 µM induced apoptosis in MCF-7 cells. Hesperidin is potentially to be developed as co-chemotherapeutic agent for breast cancer, while molecular mechanism need to be explored.  Key words: Hesperidin, doxorubicin, synergism, MCF-7, apoptosis
Co-Authors . Anindyajati . Larasati Adisusilo, Midori Rahmadhany Putri Aditya Fitriasari Aditya Fitriasari Agusta Fauzi, Ilham Agustina Setiawati Al-Qorin, Fadillah Amaliyatul, Mita Ameilinda Monikawati Andita Pra Darma Angraini, Sonia Meta Anindyajati Anindyajati Anindyajati Anindyajati Arya Nugraha, Reyhan Asep Nuryadin Astrid Ayu Maruti Astrid Ayu Maruti Azmi Rahmadani Bambang Sulistiyo Ari Sudarmanto Bani Adlina Shabrina Cyndwika Ayu Dewi Pratiwi Dewi Pratiwi Dhania Novitasari Dini Maharani Dyaningtyas D. P. Putri Dyaningtyas D.P. Putri Dyaningtyas Dewi Putri Pamungkas Ediati Sasmito Ediati Sasmito Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Erlina Rivanti Esti, Yuni Fajar Fauziyah Darwis, Rattu Syahada Fazri, Rezi Muhammad Fikri Amalia Guntara, Rangga Gelar Handani, Dewa Ayu Sri Heny Hendrayati Herwandhani Putri Ibrahim Arifin Ika Nurzijah Ikawati, Muthi' Ilham Agusta Fauzi Ilham Augusta F Ilham Augusta F. Indah Hairunisa Indri Kusharyanti Inna Armandari Jenie, Riris Istighfari Juni Ekowati Kartika Dyah Palupi Kartika Dyah Palupi Khairunnisa, Najla Laeli Muntafiah Lailatul Qodria Lailatul Qodria Larasati Larasati Luthfia Indriyani Luthfia Indriyani Maesaroh, Syti Sarah Maran, Gergorius Gena Marcellino Rudyanto Maulid, Zaki Nuraziz Meiyanto, Edy Mokh Adib Sultan, Mokh Adib Muhammad Novrizal Abdi Sahid Musyaffa, Fakhrizal Labib Muthi Ikawati Nanda Resa Pratama Nanda Resa Pratama Naufa Hanif Niken Nur W Niken Nur W, Niken Novi Hastuti Novi Hastuti, Novi Nugraha, Muhammad Rizki Nugraheni, Nadzifa Nur Fitra Sari Nurhaliza, Jelita Nurma Sabila Nurrachma, Marsya Yonna Perdana Adhi Nugroho Pratama, Dimo Purwaamijaya, Btari Mariska Putri, Nindya Budiana Rahmawati, Desty Restia Rahmi Khamsita Rahmi Khamsita Ramadani, Ratna Dwi Ratih Hurriyati Ratna Asmah Susidarti Ratna Asmah Susidarti Ratna Dwi Ramadani Retno Murwanti Ridlo, Muhammad Dzikri Ar Riris Istighfari Jenie Riris Istighfari Jenie Riris Istighfari Jenie Rita Riata Rita Riata Ritmaleni, Ritmaleni Rohmad Yudi Utomo Rosana Anna Ashari, Rosana Anna Rumiyati Rumiyati Sahid, Muhammad Novrizal Abdi Santoso, Christopher Filando Sari Haryanti Sari Haryanti Sarmoko Sarmoko Sendy Junedi Shigeru Sasaki Shigeru Sasaki Sofa Farida Sofa Farida Sukardiman Susi Ari Kristina Susi Ari Kristina Susi Ari Kristina Susi Ari Kristina Tamara, Agra Fadhilla Tutuk Budiati Yanti, Septi Dwi Yullia Febrianti, Sheilla Yurista Gilang Yurista Gilang Zahra, Nasywa