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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Journal of Food and Pharmaceutical Science Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Jurnal Teknosains Jurnal Teknologi Pertanian agriTECH INDONESIAN JOURNAL OF PHARMACY Industria: Jurnal Teknologi dan Manajemen Agroindustri Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Indonesian Food and Nutrition Progress Jurnal Perikanan Universitas Gadjah Mada Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Alwatzikhoebillah : Kajian Islam, Pendidikan, Ekonomi, Humaniora Journal of Multidisciplinary Applied Natural Science Jurnal Pengabdian Masyarakat : Pemberdayaan, Inovasi dan Perubahan Analisa: Journal of Social Science and Religion Estungkara: Jurnal Pengabdian Pendidikan Sejarah Paradigma: Jurnal Kajian Budaya Journal of Food and Pharmaceutical Sciences Journal of Fisheries & Marine
Amir Husni
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Journal : Journal of Fisheries

 1 
Optimization of Medium Composition for Streptomyces sp. PB2 Chitinase Production using Response Surface Methodology Anandita Perwita Kurniawan; Indun Dewi Puspita; Amir Husni
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 1 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i1.27602

Abstract

Highlight ResearchThe chitinase production by Streptomyces sp. PB2 was studiedThe critical medium component for chitinase production were identifiedThe optimum medium composition for chitinase production was obtainedMedium optimization improved chitinase production by 6-fold increase in activityAbstractChitin is a polysaccharide compound composed of N-acetylglucosamine (NAG), which is linked by β-1,4-glycoside bonds. In producing NAG from chitin, enzymatic method using chitinase offer advantages compared to chemical degradation. Streptomyces sp. PB2 is a good candidate of chitinase producer which was previously isolated from shrimp pond sediment. However, optimization of chitinase production by Streptomyces sp. PB2 is required for large-scale production of this enzyme. This study aimed to find the optimal medium composition to increase the chitinase enzyme activity of Streptomyces sp. PB2 using the Response Surface Method. Initial screening was done to determine additional carbon and nitrogen sources in colloidal chitin broth suitable for increasing chitinase activity. Optimization of the medium composition was conducted using the Plackett-Burman design to determine the critical components in the colloidal chitin broth medium and continued by Box-Behnken model to optimize the concentration of the medium components. Chitinase activity was obtained by measuring the amount of reducing sugar (NAG) released from enzymatic reaction using DMAB reagent by means of spectrophotometer. The medium components showing high contribution in increasing chitinase activity were K2HPO4, colloidal chitin and peptone, with the confidence level value of 0.66, 0.48, and 0.38, respectively. The Box-Behnken model analysis shows that the combination of K2HPO4 0.007 g/ml, colloidal chitin 1.5 g/ml and peptone 1.5 g/ml in colloidal chitin broth are the optimal medium for Streptomyces sp. PB2, resulted in chitinase activity of 0.0125 U/ml. The increase of 6-fold in chitinase activity was achieved in this study.
Extraction, Purification, and Bioactivity of Fucoxanthin from Brown Seaweed Sargassum hystrix Mufarocha, Nungky; Husni, Amir; Putra, Masagus Muhammad Prima
Jurnal Ilmiah Perikanan dan Kelautan 2026: JIPK VOLUME 18 ISSUE 2 YEAR 2026 (JUNE 2026, ISSUE IN PROGRESS)
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i2.84197

Abstract

Graphical Abstract Highlight Research 1. UAE at 45°C for 30 min produced the highest crude extract yield (19.90%). 2. Ethyl acetate:n-butanol (8:2) yielded the highest fucoxanthin fraction (1.31 mg/g). 3. Fucoxanthin fractions showed strong antioxidant activity (DPPH IC₅₀ 86.18±5.06 µg/mL). 4. Purified fractions exhibited strong tyrosinase inhibition (IC₅₀ 76.04±3.24 µg/mL).   Abstract Fucoxanthin is the major carotenoid present in brown seaweeds and exhibits strong biological activities, particularly as an antioxidant and anti-aging agent. However, efficient extraction and purification conditions for fucoxanthin from Sargassum hystrix, along with its functional evaluation, remain limited. This research aimed to establish the most effective extraction conditions for obtaining fucoxanthin from S. hystrix using Ultrasound‑Assisted Extraction (UAE), determine the optimal solvent system for purification, and evaluate the antioxidant and anti-aging activities of both the extracted and purified fucoxanthin. Extraction was carried out using UAE with 96% ethanol at temperatures of 35–45°C and durations of 20–30 min. Purification was conducted through column chromatography using three solvent systems: n‑hexane:acetone (6:4 v/v), methanol:chloroform (7:3 v/v), and ethyl acetate:n‑butanol (8:2 v/v). Fucoxanthin was identified by thin-layer chromatography and fucoxanthin was quantified using reversed-phase high-performance liquid chromatography (HPLC) equipped with a C18 column, while antioxidant activity was measured using the DPPH method and anti-aging activity was assessed via tyrosinase inhibition. The results showed that optimal extraction occurred at 40–45°C for 25–30 min, yielding 18.33–19.9% crude extract, and the ethyl acetate:n‑butanol (8:2 v/v) system produced the highest fucoxanthin fraction yield (8.26–9.54%), with fraction F22 containing the highest fucoxanthin level at 1.31 mg/g. The fucoxanthin fraction displayed markedly enhanced activity, with strong antioxidant capacity (IC₅₀ = 86.18±5.06 μg/mL, an 83.8% improvement over the crude extract) and strong tyrosinase inhibition (IC₅₀ = 76.04±3.24 μg/mL). Overall, fucoxanthin derived from S. hystrix demonstrates substantial promise as an active antioxidant and anti-aging compound for nutraceutical and cosmetic applications, suggesting that future studies should focus on scaling up the extraction and purification process and further elucidating its mechanisms of action and stability in formulated products.  
Co-Authors Ade Octavia Adi Saputra, Muhamad Agung Endro Nugroho Agung Endro Nugroho Agung Giri Samudra Akhmad Awaludin Agustiar Al-Fairusy, Muhajir Alim Isnansetyo ALIM ISNANSETYO Almira Islamei Pratiwi Alvika Hayyu Chandra Permana, Alvika Hayyu Chandra Amelia Hana Amir Mugozin Anandita Perwita Kurniawan Andhifani, Wahyu Rizky Andi Hakim Annisa Ajeng Maharani Anny Wahyuni Antarif Kusuma Brata Atah, Said Azizi, Wirdatul Auliya Bambang Retno Aji Bekti Wulan Sari Bekti Wulan Sari Benny Agusti Putra, Benny Agusti Bustami Abubakar Claude Mona Airin Deffy R. Putra Dewi Ariani Donghwa Chung Dwi Retno Wulandari Ekantari, Nurfitri Endang Sri Rahayu Ervika Rahayu Novita Herwati Fatimah, Aden Nurin Fitriaty, Fitriaty Gandhi E. Julianto Ginting, Rini Febriyanta Br Hafiful Hadi Sunliensyar Hanum, Maulida Selma Hermansyah Hermansyah Il Shik Shin Imam Arda Perdana Imas Faturrohmah Indriani, Rizka Indun Dewi Puspita Indun Dewi Puspita Ivana M. Diharningrum Iwan Y.B. Lelana Iwan Y.B. Lelana Iwan Yusuf B. Lelana Iwan Yusuf Bambang Lelana Iwan Yusuf Bambang Lelana Iwan Yusuf Bambang Lelana Komala Putu Tara Hradaya Kristiani, Theresia Adven Dea Kun Cahyaningrum Latif Sahubawa Leihitu, Irsyad Lupi Purnomosari Maknun, Fazidatul Lu’luil Maria Madalena Maulida Selma Hanum Mohamad Gazali Mufarocha, Nungky Muhammad Adi Saputra Muhammad Nursid Mustofa Meihan, Andre N. Nurjanah Nasruddin AS, Nasruddin AS Nurfitri Ekantari Nurfitri Ekantari Nurul Binti Isnaini Nurul Binti Isnaini Obdulia P. Nugrahani Pingkan Mayestika Afgatiani Pris Larasati Pudji Astuti PUDJI ASTUTI Pudji Astuti Punky Kusuma Damayanti Putra, Hemi Adi Putra, Masagus Muhammad Prima Radipta Lailatussifa Rafiie, Said Achmad Kabiru Ramadhan, Kurnia Fajar Rani Laksanawati Reza Idria Riky Paskandani, Riky Rina Syafitri, Rina Risa Ummami Rizka Indriani Rizqi Wahyu Herdianto Ruhamah Ruhamah Rusyda Nurshitaningrum Sadzali, Asyhadi Mufsi Said Achmad Kabiru Rafiie Said Atah Sanusi Ismail Sarmin Sarmin Setiadi, May Khoirunnisa Siti A. Budhiyanti Siti Ari Budhiyanti Sovia Indah Nurkhanifah Subaryono Subaryono Subaryono Subaryono, Subaryono Suhardani, Mila Novalita Sunandar, Husna Vannisa Taswir Taswir Tazwir Tazwir Titin Agustin Nengsih Triyanto Triyanto Ustadi Ustadi Ustadi Ustadi Ustadi Ustadi Ustadi Ustadi Ustadi, Ustadi Uswatun Hasanah Yudi Pranoto Yusuf Kalingga Murda Zuriat, Zuriat