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All Journal HAYATI Journal of Biosciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology The Journal of Experimental Life Sciences (JELS) Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Jurnal EECCIS Journal of Innovation and Applied Technology Research Journal of Life Science Journal of Environmental Engineering and Sustainable Technology The Indonesian Green Technology Journal Jurnal Pembangunan dan Alam Lestari REKAYASA JFMR (Journal of Fisheries and Marine Research) Omni-Akuatika Jurnal Penelitian Pendidikan IPA (JPPIPA) Samakia: Jurnal Ilmu Perikanan PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Journal of Aquaculture and Fish Health Jurnal Akuakultura Universitas Teuku Umar JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Jurnal Pengabdian pada Masyarakat Ilmu Pengetahuan dan Teknologi Terintegrasi Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine
Maftuch Maftuch
Faculty Of Fisheries And Marine Science, Brawijaya University, Malang, Indonesia
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Journal : JFMR (Journal of Fisheries and Marine Research)

 1 
THE EFFECTIVITY OF RAW EXTRACT GUAVA LEAF IMMUNOSTIMULANT (Psidium guajava) ON LIVER HISTOPATHOLOGY OF PATIN (Pangasius sp.) TESTED THE CHALLENGE Aeromonas hydrophila BACTERIA Ihsan Sanggar Rahman; Mr. Maftuch; Ellana Sanoesi
JFMR (Journal of Fisheries and Marine Research) Vol 2, No 2 (2018): JFMR VOL 2 NO 2
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8433.607 KB) | DOI: 10.21776/ub.jfmr.2018.002.02.1

Abstract

The purpose of this study described the influence and the dose of guava leaf raw extract (P. guajava) as an immunostimulant towards liver histotapologi Patin (Pangasius sp.) Challenge tested A. hydrophila bacteria. This study uses a completely randomized design (CRD) with 4 treatments and 3 replications. The treatments used was of guava leaf extract doses A dose of 2% (2.4 g / 120 g), and 4% (4.8 g / 120 g), C 6% (7.2 g / 120 g) and K without a guava leaf extract and tested bacteria A. hydrophila challenge. Based on the results obtained in the study of liver histopathology Patin (Pangasius sp.), there was damage Inflammation and necrosis of the liver tissue in order from the most severe damage to the least obtained at treatment K (0%) 3,07, A ( 2%) 2.53, B (4%) of 2.33, and the best treatment is C (6%) with an average damage of 1.67. Necrosis of liver tissue damage in a sequence of severe damage to the least, namely the treatment of K (0%) 2,67, A (2%) 2.33, B (4%) 2.20, and C (6%) 1,53. Lowest average gained in treatment C (6%). The results showed that the dose can reduce the level of tissue damage that was at a dose of 6% (7.2 g / 120 g). The best dose is 6% (7.2 g / 120 g), which can reduce the level of damage indicate the level of tissue damage smaller percentage of damage 6-25%.
UJI DAYA HAMBAT EKSTRAK Chaetoceros calcitrans TERHADAP BAKTERI Aeromonas salmonicida Mr. Maftuch; Febby Hadi Setyawan; Heny Suprastyani
JFMR (Journal of Fisheries and Marine Research) Vol 2, No 1 (2018): JFMR VOL 2 NO 1
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.748 KB) | DOI: 10.21776/ub.jfmr.2018.002.01.6

Abstract

The impact of use antibiotic can cause problems on aquaculture such as bacterial resistance and residues for consumer. Therefore, one of alternatives that can be used to inhibit or kill bacteria, one of which is the use of plankton Chaetoceros calcitrans. This research aims to know the influence of the granting C. calcitrans extract that can inhibit or kill bacteria and know the best dose for inhibit A. salmonicida bacteria. The experiment method  used randomize completely design consist of four treatment and two control. A treatment (0.3 ppm), B treatment (15.3 ppm), C treatment (30.3 ppm),  D treatment (45.3 ppm), positive control (add chlorampenicol 5ppm) and negative control (without extract). The result of research showed the highest average of clear zone diameter in D treatment 5.37 mm and the lowest average of clear zone diameter in A treatment 2.89 mm, with linear equation y = 3.2067 + 0.0509x and coefficients R2 = 0.9262. The relationship between C. calcitrans extract in inhibitng gwoth of A. salmonicida bacteria showed that the average diameter of the clear zone has increase in line with the addition of extracts. Form the research showed that the extract of C. calcitrans was bacteriostatic (inhibits the growth of bacteria) and best obtained at the treatment dosage of D with a dose of 45.3 ppm and included into the category of middle.
Uji Viabilitas Kandidat Vaksin Bakteri Aeromonas Hydrophila Menggunakan Metode Formalin-Killed dengan Konsentrasi Berbeda: Viability Assay of Aeromonas Hydrophila as Candidate Vaccine Using Formalin-Killed Method at Different Concentration Herlina, Cucun; Maftuch, Maftuch; Fadjar, Mohamad
JFMR (Journal of Fisheries and Marine Research) Vol. 9 No. 1 (2025): JFMR on March
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2025.009.01.7

Abstract

Salah satu upaya pencegahan yang dapat dilakukan untuk mengatasi penyakit pada ikan yaitu menumbuhkan kekebalan seperti vaksinasi karena kekebalan tubuh yang meningkat pada ikan dapat mencegah infeksi penyakit. upaya telah dilakukan untuk mengembangkan program imunisasi yang efektif dengan menggunakan vaksin yang dibuat dari sel utuh bakteri yang diinaktivasi menggunakan formalin dan/atau pemanasan Tujuan penelitian ini untuk mengetahui hasil uji viabilitas dan hasil uji adhesi sel epitel usus menggunakan vaksin inaktif formalin dengan konsentrasi berbeda. Metode dalam penelitian ini adalah metode eksperimen dengan rancangan percobaan menggunakan rancangan acak lengkap (RAL) terdiri dari 3 perlakuan (A=2%, B=3%, dan C=4%) dan kontrol dengan 3 kali ulangan. Hasil penelitian diperoleh hasil kandidat vaksin inaktif bakteri A.hydrophila sebanyak 9 ml/tabung falcon. Infeksi bakteri A. hydrophila dengan kepadatan 109 cfu/ml sebanyak 0,1 ml dengan masa pemeliharaan 1 minggu ditandai terjadi pengerasan di bagian perut dan terdapat kerusakan pada sirip punggung pada hari ketujuh. Hasil uji viabilitas menunjukkan tidak terdapat pertumbuhan koloni bakteri A. hydrophila dibandingkan dengan kontrol. Hasil uji adhesi sel epitel usus pada kontrol dan perlakuan A, B, C diperoleh rata-rata 10.3, 0.73, 0.43, dan 0.23 bakteri/10 sel epitel. Analisis uji ANOVA diperoleh nilai uji F Hitung > F Tabel, artinya perlakuan memberikan pengaruh sangat berbeda signifikan antar semua perlakuan. Hasil analisa regresi diperoleh (R2) sebesar 0.66 bahwa hubungan keterkaitan antara vaksin inaktif konsentrasi formalin berbeda dengan jumlah bakteri A. hydrophila yang menempel pada sel epitel sebesar 66%.   One preventive effort to tackle diseases in fish is by boosting immunity, such as through vaccination, as increased immunity in fish can prevent disease infections. Efforts have been made to develop effective immunization programs using vaccines made from whole bacterial cells inactivated using formalin and/or heat. The objective of this study was to determine the results of viability tests and intestinal epithelial cell adhesion tests using formalin-inactivated vaccines with different concentrations. The method used in this study was an experimental approach with a completely randomized design (CRD) consisting of three treatments (A=2%, B=3%, and C=4%) and a control, each with three replications. The study results showed that the candidate inactivated vaccine of A. hydrophila bacteria was obtained at 9 ml/falcon tube. A. hydrophila bacterial infection with a density of 10⁹ CFU/ml at 0.1 ml and a maintenance period of one week was characterized by abdominal hardening and dorsal fin damage on the seventh day. The viability test results showed no bacterial colony growth of A. hydrophila compared to the control. The intestinal epithelial cell adhesion test results for the control and treatments A, B, and C showed average bacterial adhesion of 10.3, 0.73, 0.43, and 0.23 bacteria/10 epithelial cells, respectively. ANOVA test analysis showed that the calculated F-value was greater than the table F-value, indicating a highly significant effect among all treatments. Regression analysis resulted in an R² value of 0.66, suggesting that 66% of the variation in the adhesion of A. hydrophila bacteria to epithelial cells was associated with different formalin concentrations in the inactivated vaccine.
Co-Authors A'yunin, Qurrota Abdul Rahem Faqih Achmad Noercholis Ade Yamindago Agus Budiarto Ahmad Fahrul Syarif Amira Baihani Andhang Sebastian Andriyanto, Septyan Annisa Fitri Anggreini Ari Wahjudi Arief Prajitno Arif Hoetoro Arning Wilujeng Ekawati Asep A. Prihanto Ash Shufi, Abdillah Hanan Astuti, Retno Tri Asus Maizar Suryanto H Bramantiyo Satriyo Wicaksosno Cucun Herlina D. Ardiansyah Daniar Kusumawati Desy Setyoningrum Dwi R. Fatmawati E. Sanoesi Ellana Sanoesi Endang Dewi Masithah Fadjar, Mohammad Farikhah, Reghita Dwi Febby Hadi Setyawan Febi Kesid Haryo Wibisono Febi Nadhila Nurin Friyuanita, Friyuanita Gede Angga Krishna Fariestha H. Suprastyani Happy Nursyam Hariati, Anik Martinah Heny Suprastyani Heny Suprastyani Heny Suprastyani, Heny Herlina, Cucun Hermawan Meidy Kurnianto Ihsan Sanggar Rahman Ika K. Nisyak Immaria Fransira Ismaningdyah Kurniawati Juanda, Eki Karimah Karimah Lila Gardenia M. Aziz Muslim Marsoedi Marsoedi Mauludia, Farida Mochamad Awaludin Adam Moh. Awaludin Adam MOHAMAD FADJAR Mohammad Bisri Mohammad Holil Lulloh Muhammad Arif Nasution Muhammad Arif Nasution Muhammad Awaluddin, Muhammad Muhammad Dailami, Muhammad Muhammad Musa Muhammad Musa Muhammad Nursid N. Farida N. Habibah Nabilla Anggi Juliyanto Nafiqoh, Nunak Novita, Hessy Nuranti Anarkhis Nurin, Febi Nadhila Pratama, Vian Dedi Prihanto, Asep A. Putra, Gede A. A. K. Putra, Mirsa F. R. Rinaldi Redi Bintarto, Redi Rudianto Raharjo Runi Asmaranto Samuki, Khairul Sanoesi, Ellana Sebastian, Andhang Setyawan, Fahreza Okta Soelistyoadi, Rachmat Noer Sri Andayani Sri Andayani Sri Rahayu Sri Widyarti Sugiarto S Suhermanto, Achmad Sukarni Sukarni Supriatin, Febriyani Eka Syarifah Hikmah J. S Teguh Dwi Widodo Tirani, Elisabeth Vendrita Damayanti Putri Veryl Hasan W., Denny Widya Yahya Yahya Yanuhar, Uun Yenny Risjani Yuni Kilawati Yusuf Arif Wahyudi Zulfadhli Zulfadhli Zulfadhli Zulfadhli