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All Journal HAYATI Journal of Biosciences VALENSI Bionatura Indonesian Journal of Applied Sciences Microbiology Indonesia Majalah Kedokteran Bandung Indonesian Journal of Pharmaceutical Science and Technology Chimica et Natura Acta ALCHEMY Jurnal Penelitian Kimia JURNAL SAINS INDONESIA Jurnal Pendidikan Kimia (JPKim) Indonesian Journal of Chemistry Pharmaceutical Sciences and Research (PSR) Jurnal Sains dan Teknologi Farmasi Indonesia Kimia Padjadjaran

Iman Permana Maksum

Departemen Kimia, FMIPA, Universitas Padjadjaran

Author-ID : 286101

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Temperature effect on expression of recombinant human prethrombin-2 in Escherichia coli BL21(DE3) ArcticExpress Saronom Silaban; Murniaty Simorangkir; Shabarni Gaffar; Iman Permana Maksum; Toto Subroto
Jurnal Pendidikan Kimia (JPKim) Vol 11, No 3 (2019): December
Publisher : Pascasarjana Universitas Negeri Medan

Many proteins produced in E. coli accumulate in inclusion bodies. This study aims to detect the role of temperature in reducing the formation of inclusion bodies during recombinant human prethrombin-2expressed in E. coli BL21 (DE3) Arctic Express host. In this study, we created a host growth curve to find out the right time to add an inducer. The inducer used in our experiment was IPTG 0.1 mM. The fermentation process use a temperature of 12°C and 22°C. The results showed that recombinant human prethrombin-2 was successfully expressed as protein soluble using an optimum temperature of 12°C in E. coli BL21 (DE3) Arctic Express. It was indicated from the 63kDa protein band obtained from the soluble fraction on SDS-PAGE. The higher temperature of fermentation increased the amount of protein in the insoluble fraction due. It concluded that the fermentation temperature affect the rate of prethrombin-2 expression.Keywords: E. coli BL21(DE3) ArcticExpress, prethrombin-2, soluble, temperature

Studi Mutasi Titik A3243G DNA Mitokondria Penyebab Maternally Inherited Diabetes and Deafness Sriwidodo, Sriwidodo; Suprijana, O; Subroto, Toto; Maksum, Iman Permana
Majalah Ilmu Kefarmasian Vol. 5, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Point mutation of mitochondrial DNA A3243G has been known as a cause of Mater-nally Inherited Diabetes and Deafness (MIDD). Potency of MIDD can be identified from patient phenotype of Non Insulin Dependent Diabetes Mellitus (NIDDM). The objective of this study is acquiring information about MIDD on patient of NIDDM type and obtaining the simple method to detect the point mutation of mtDNA A3243G. 50 NIDDM patients were attained from RSCM Hospital, Jakarta. Information con-cerning family history with NIDDM and existences of deafness, medication, and other complication and manifestation were obtained through interview and ques-tioner. Point mutation of A3243G was determined with the method of PCR Allele’s Specific Amplification (PASA) Mismatch 2 bases and PCR-Restriction Length Poly-morphism (PCR-RFLP) with the HaeIIl restriction enzyme. Detectable Potency MIDD was found by perceiving the patient phenotype and identifying the mutation of heteroplasmic A3243G utilizing the PASA method.

Optimization of Aptamer-Based Electrochemical Biosensor for ATP Detection Using Screen-Printed Carbon Electrode/Gold Nanoparticles (SPCE/AuNP) Rahmaniar Mulyani; Nida Yumna; Iman Permana Maksum; Toto Subroto; Yeni Wahyuni Hartati
Indonesian Journal of Chemistry Vol 22, No 5 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.72820

Electrochemical biosensors are used to detect adenosine triphosphate (ATP) levels, which are involved in a variety of biological processes, such as regulating cellular metabolism and biochemical pathways. Therefore, this research aims to develop an aptamer-based electrochemical biosensor with Screen Printed Carbon Electrode/gold nanoparticles (SPCE/AuNP) and collect data as well as information related to ATP detection. The modification of SPCE with AuNP increased the analyte’s binding sensitivity and biocompatibility. The aptamer was selected based on its excellent bioreceptor characteristics. Furthermore, aptamer–SH (F1) and aptamer-NH2 (F2) were immobilized on the SPCE/AuNP surface, which had been characterized using SEM, EIS, and DPV. Also, the ATP-binding aptamers were electrochemically characterized using the K3[Fe(CN)6] redox system and Differential Pulse Voltammetry (DPV). According to the optimization results using the Box-Behnken experimental design, the ideal conditions obtained from the factors influencing the experiment were the F1 concentration and incubation time of 4 µM and 24 h, respectively, as well as F1/F2/ATP incubation time of 7.5 min. Meanwhile, for the range of 0.1 to 100 µM, the detection (LoD) and quantification (LoQ) limits were 7.43 and 24.78 µM, respectively. Therefore, this aptasensor method can be used to measure ATP levels in real samples.

Biosensor Elektrokimia untuk Memonitor Level Hemoglobin Terglikasi (HbA1c) pada Penyakit Diabetes Melitus Sheila Destiani; Iman Permana Maksum; Yeni Wahyuni Hartati
ALCHEMY Jurnal Penelitian Kimia Vol 19, No 1 (2023): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.19.1.58439.94-107

Hiperglikemia merupakan ciri diabetes melitus, sekelompok penyakit metabolik yang disebabkan oleh kelainan sekresi insulin, kerja insulin, atau keduanya. Hiperglikemia dapat menyebabkan kerusakan jangka panjang, disfungsi, dan kegagalan berbagai organ, terutama jantung, mata, saraf, pembuluh darah, dan ginjal. Oleh karena itu, diagnosis dan pemantauan rutin kadar glukosa darah sangat penting dilakukan. Hemoglobin terglikasi (HbA1c) adalah hemoglobin yang berikatan dengan glukosa dalam darah dan ditetapkan menjadi biomarker hiperglikemia yang dapat mengindikasikan diabetes atau prediabetes. Akan tetapi, pengukuran HbA1c di laboratorium klinis membutuhkan instrumen yang canggih dan mahal, tenaga ahli khusus dan waktu lama. Oleh karena itu, biosensor elektrokimia kemudian mulai dikembangkan untuk mendeteksi kadar HbA1c karena mudah digunakan, hasil cepat, dan harga yang murah. Ulasan ini membahas biosensor elektrokimia untuk mendeteksi HbA1c dan metode imobilisasi bioreseptor.Electrochemical Biosensor for Monitoring Glycated Hemoglobin (HbA1c) Levels in Diabetes Mellitus. Hyperglycemia is a hallmark of diabetes mellitus, a group of metabolic diseases caused by defects in insulin action, insulin secretion, or both. Hyperglycemia can cause long-term damage, dysfunction, and failure of various organs, especially the heart, blood vessels, eyes, nerves, and kidneys.. Therefore, routine diagnosis and monitoring of blood glucose levels are very important. Glycated hemoglobin (HbA1c) is hemoglobin that binds to glucose in the blood and is established as a biomarker of hyperglycemia that may indicate diabetes or prediabetes. However, measurement of HbA1c in the clinical laboratory requires large and expensive instruments, specialized experts, and takes a long time. Therefore, an electrochemical biosensor began to be developed to detect HbA1c levels because it is easy to use and has fast results at lower prices. This review discusses electrochemical biosensors for detecting HbA1c and the immobilization of bioreceptors.

Codon Optimization and Chaperone Assisted Solubilization of Recombinant Human Prethrombin-2 Expressed in Escherichia coli SARONOM SILABAN; IMAN PERMANA MAKSUM; SHABARNI GHAFFAR; KHOMAINI HASAN; SUTARYA ENUS; TOTO SUBROTO; SOETIJOSO SOEMITRO
Microbiology Indonesia Vol. 8 No. 4 (2014): December 2014
Publisher : Indonesian Society for microbiology

Prethrombin-2 (PT2) is a thrombin precursor, which plays a role in the conversion of fibrinogen into fibrin during blood clotting process. Previous study reported that the expression of human prothrombin-2 (rhPT2) in Escherichia coli formed inclusion bodies. The aim of this study was to establish a strategy to express a soluble rhPT2 in E. coli. This study was animed to design and codon optimize human prethrombin-2 gene as well as to optimize the expression condition using four strains of E. coli. The codon adaptation index (CAI) of the unoptimized hpt2 gene was 0.336, with 56.8% GC content. After optimization, the CAI of optimized hpt2 became 1.000 with 53.1% GC content. The optimized gene was successfully cloned into pTWIN1 expression vector. Expression analysis indicated that only E. coli ArcticExpress strain could successfully express a soluble recombinant rhPT2 protein, with only part of rhPT2 being expressed in insoluble form. However, the rest of the E. coli strains used in the experiments failed to express the rhPT2 in soluble form. We are deducing that the success in achieving soluble expression was not only due to the availability of chaperonins Cpn60/Cpn10, which played a crucial role in the protein folding in E. coli ArcticExpress strain, but also due to the codon optimization of hpt2 gene.

Study on the Mitochondrial Genome of Variants Carrying mt.3243A>G from Type-2 Diabetes Mellitus and Cataract Patients in Indonesia Iman Permana Maksum; Rahmaniar Mulyani; Khomairi Hasan; Mamlikatu Ilmi Azizah; Wanda Destiarani; Ahmad Fariz Maulana; Muhammad Yusuf; Toto Subroto
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1017-1024

The association of type-2 diabetes mellitus (T2DM) and cataract with mtDNA mutation has been reported before. Despite the high prevalence of DM and cataract in Indonesia, a study of the mtDNA variants in Indonesia in correlation with the two diseases is still limited. MT.3243A>G is one of the hotspots mutations for mitochondrial diseases, but the explanation for its occurrence in patients with pure cataract is still elusive. Therefore, the objective of this study was to analyze the mitochondrial genome variants from T2DM and cataract patients in Indonesia using the direct sequencing method. The homology analysis of the genome to the Cambridge reference sequence resulted in 86 variants, including 20 variants that cause amino acid substitutions. Based on the Mitomap data, 17 of the 20 variants were novel. Upon comparison with the 12 normal variant genomes, 11 of 17 variants were suggested to be associated with T2DM and cataract diseases since they code the protein in complex-I (ND4L, ND5, and ND6), complex-III (cytb), and complex-V (ATP6) of the respiratory complex. Interestingly, MT.3316G>A, for the first time, is shown in a pure cataract patient. In addition, the novel phenotype of MT.5460G>A and MT.10398A>G were revealed, which are T2DM and cataract in one patient. Based on our study, these diseases might be related to the disruption of the ATP metabolism due to the structure and function changes of proteins involved in the respiratory complex. This discovery is expected to offer an understanding of the origins of gene-level clinical differences, particularly in Indonesia.

Review Article: Effect of Co-Expression Chaperones on the Expression of Intracellular Recombinant Proteins in Escherichia coli Diandra Firdiani Utami; Mamlikatu Ilmi Azizah; Sriwidodo Sriwidodo; Ryan Adibagus Haryanto; Riyona Desvy Pratiwi; Iman Permana Maksum
Chimica et Natura Acta Vol 11, No 2 (2023)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v11.n2.46480

Escherichia coli is widely used as a host for expressing recombinant proteins due to its well-studied genetics, fast growth, relatively low production costs, and high rate of protein expression. However, despite the high rate of protein expression, the availability of chaperone proteins was often insufficient, resulting in the formation of inclusion bodies due to errors in protein folding. These inclusion bodies can cause the protein to become inactive, and proper protein folding is crucial for maintaining the structure and function of proteins in living organisms. To overcome this limitation, chaperones have been developed as a strategy to help prevent protein folding errors and increase the recovery of soluble protein. In this review, we summarize several experiments related to co-expressing chaperones to enhance the expression of recombinant proteins in E. coli.

OPTIMASI EKSPRESI SCFV-BAD ANTI-NS1 VIRUS DENGUE PADA ESCHERICHIA COLI ORIGAMI MENGGUNAKAN RESPONSE SURFACE METHOD Astriany, Dewi; Effendi, Syulastri; Febriani Aji Kusuma, Sherlynda; Kusumawardhani, Shinta; Permana Maksum, Iman; Natalia, Dessy; Subroto, Toto
JURNAL SAINS DAN TEKNOLOGI FARMASI INDONESIA Vol 11, No 1 (2022)
Publisher : Sekolah Tinggi Farmasi Indonesia

Dengue merupakan penyakit menular yang masih menjadi masalah kesehatan di dunia. Manifestasi klinis infeksi dengue sulit dibedakan dengan penyakit infeksi lainnya. Uji diagnostik infeksi virus dengue yang cepat dan akurat sangat diperlukan untuk konfirmasi penyakit dan penanganan pasien yang tepat. NS1 adalah glikoprotein yang paling imunogenik dan lestari, disekresikan ke dalam aliran darah. Oleh karena itu, pemeriksaan antigen dari virus dengue NS1 telah diidentifikasi sebagai salah satu penanda spesifik dalam uji diagnostik laboratorium, yang dapat digunakan untuk mendeteksi infeksi dengue primer atau sekunder pada stadium awal. Penelitian ini bertujuan untuk menentukan kondisi optimum ekspresi protein fusi scFv-BAD rekombinan untuk deteksi antigen NS1 virus dengue menggunakan Response Surface Method. Ekspresi protein diinduksi oleh berbagai konsentrasi IPTG (0,1, 0,5, dan 1 mM) pada beberapa suhu selama 18 jam dalam medium Luria Bertani dengan penambahan biotin. ScFv terbiotinilasi rekombinan yang dimurnikan dikarakterisasi dengan metode SDS-PAGE. Hasil penelitian menunjukkan bahwa protein fusi scFv-BAD rekombinan sebagian berada dalam bentuk terlarut dan sebagian berupa badan inklusi. Analisis Central Composite Design menunjukkan bahwa konsentrasi IPTG yang sesuai untuk memproduksi protein scFv rekombinan adalah 0,5 mM pada suhu 28 ºC pada media Luria Bertani.

In Silico Study of Aptamer Specificity for Detection of Insulin as Development for Diabetes Mellitus Diagnosis Mulyani, Dinda Exelsa; Maksum, Iman Permana; Yusuf, Muhammad
Indonesian Journal of Chemistry Vol 24, No 3 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.91602

Diabetes mellitus (DM) is a metabolic disorder characterized by elevated blood glucose levels. There are 2 types of DM where molecular-level diagnosis becomes very important because both have different treatments to avoid treatment errors. An electrochemical aptasensor as a type 2 DM detector with insulin target has been developed. This study aims to determine the interaction and specificity based on the values of RMSD, RMSF, and binding energy between aptamer and insulin when it reaches stability in silico compared to HbA1c and glucose. Docking simulations were performed on the HDOCK webserver and dynamics simulations for 1000 ns on the aptamer and protein molecular models used. The simulation results were analyzed to see the stability and visualized using VMD to see the conformation of the aptamer-ligand complex. The docking result showed higher binding energy between aptamer-insulin compared to other molecules, namely −221.87 kcal/mol. The results of RMSF and RMSD analysis of molecular dynamics simulations show that the system is stable, has the best binding energy value of −9.9510 kcal/mol. The aptamer complex with insulin showed better specificity compared to glucose and HbA1c based on RMSD, RMSF, and binding energy.

Review: Mutasi DNA Mitokondria Penyebab Penyakit Mitokondria dan Diagnosis Mekanisme Molekulernya melalui Pendekatan In Silico Setiawan, Topan; Maksum, Iman Permana; Yusuf, Muhammad
Kimia Padjadjaran Vol 2, No 1 (2023)
Publisher : Kimia Padjadjaran

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Mitokondria, organel sel yang menghasilkan energi dalam bentuk ATP melalui sistem respirasi, memiliki DNA tersendiri (mtDNA) yang rentan terhadap mutasi karena tingginya laju replikasi. Mutasi pada mtDNA dapat menyebabkan penyakit mitokondria. Kajian ini merinci karakteristik mutasi mtDNA, penyakit mitokondria, dan kajian insiliko mutasi tersebut. Disfungsi mitokondria dipicu oleh serangan radikal bebas dan mutasi mtDNA, yang dapat mengakibatkan penurunan produksi ATP dan kerusakan oksidatif pada mitokondria. Penyakit mitokondria seperti diabetes, MELAS, LHON, MERRF, dan MIDD disebabkan oleh mutasi spesifik pada mtDNA. Penelitian in-silico menggunakan simulasi dinamika molekuler, seperti mutasi T15663C dan A3243G serta mutasi lainnya yang dibahas pada kajian ini, membantu memahami dampak mutasi pada struktur dan fungsi mtDNA. Kajian tentang mutasi mtDNA yang terus berkembang penting untuk pengembangan metode diagnostik dan terapeutik. Simulasi Penelitian menggunakan metode insiliko diharapkan dapat menjadi alat diagnostik untuk mutasi yang terkait dengan sistem transfer elektron dan translokasi proton dalam penyakit mitokondria.

Co-Authors Abubakar Sidik Abubakar Sidik Ace Tatang Hidayat Ade Sholeh Hidayat Agus Safari Agus Safari Ahmad Fariz Maulana Alifa I. Nabila Anne Carolina Astrid, Dewi Azizah, Mamlikatu Ilmi Budiantoro, Ogi Carolina, Anne D Agus Yusuf Wildan DESSY NATALIA Dewi Astriany Dewi Astrid Dian Siti Kamara Diandra Firdiani Utami DWIRYANI ARIZONA Effendi, Syulastri Euis Julaeha Euis Julaeha Febriani Aji Kusuma, Sherlynda Febrianti, Inky Hidayat, Ade Sholeh Iman Rahayu Inky Febrianti KHOMAINI HASAN Khomairi Hasan Kusumawardhani, Shinta Mamlikatu Ilmi Azizah Mamlikatu Ilmi Azizah Muhammad Yusuf Muhammad Yusuf Mulyani, Dinda Exelsa Mulyani, Rahmaniar Mulyasari, Rita Murniaty Simorangkir Murniaty Simorangkir Nida Yumna O Suprijana O Suprijana, O Ogi Budiantoro Pertiwi, Wulan Purba Upay R. Ukun M.S. Soedjanaatmadja Rachman, Saadah Diana Rahmaniar Mulyani Rahmaniar Mulyani Rifky W. Rachman Rita Mulyasari Riyona Desvy Pratiwi Rizki Amalia Ryan Adibagus Haryanto Saadah Dian Rachman Saadah Diana Rachman Safri Ishmayana Sari Agung, Syennie Saronom Silaban Saronom Silaban SARONOM SILABAN Saronom Silaban Setiawan, Topan Shabarni Gaffar Sheila Destiani SOETIJOSO SOEMITRO Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Sriwidodo B, Sriwidodo Sriwidodo Sriwidodo Sriwidodo Sriwidodo Sriwidodo, . Suhaili Suhaili Sunan M. Alpiansyah SUTARYA ENUS Sutarya Enus Sutarya Enus Sutarya Enus TATI NURHAYATI Toto Subroto Upay, Purba Wanda Destiarani Wulan Pertiwi Yanyan Mulyana Yeni Wahyuni Yeni Wahyuni Hartati

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