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All Journal Jurnal Teknosains MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Majalah Kedokteran Gigi Indonesia Dentino: Jurnal Kedokteran Gigi Padjadjaran Journal of Dentistry Indonesian Journal of Prosthodontics Jurnal Dharma Indonesia

Titik Ismiyati

Departement Of Prosthodontics, Faculty Of Dentistry, Universitas Gadjah Mada, Yogyakarta

Author-ID : 2872846

Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Dentistry Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Public Health Other

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Pengaruh Etsa Kimia dengan Akua Regia terhadap Kekuatan Tarik Perlekatan Bahan Resin Akrilik pada Gigi Tiruan Kerangka Logam M Th Esti Tjahjanti; Heriyanti Amalia Kusuma; Titik Ismiyati; Erwan Sugiatno
Majalah Kedokteran Gigi Indonesia Vol 19, No 1 (2012): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Latar belakang. Bahan yang dipakai untuk pembuatan Gigi Tiruan Sebagian (GTS), antara lain: resin akrilik, kerangka logam, kombinasi kerangka logam dengan resin akrilik. Resin akrilik adalah bahan plat gigi tiruan yang memliki warna dan translusensi baik tetapi sifat mekanismenya tidak ideal, tidak tahan terhadap abrasi, dan dapat terjadi perubahan dimensi. GTS kerangka logam cukup kuat, tetapi estetis kurang memuaskan sehingga perlu kombinasi kerangka logam dan resin akrilik. Peningkatan daya lekat antara kedua bahan tersebut memerlukan retensi, teknik etsa kimia dengan akua regia dapat membuat retensi mikro pada logam. Tujuan penelitian untuk mengetahui etsa kimia dengan akua regia terhadap kekuatan tarik perlekatan bahan resin akrilik pada gigi tiruan kerangka logam. Metode penelitian. Penelitian dilakukan pada 20 subyek penelitian berupa plat kobalt kromium dengan mesh dilekati resin akrilik (10x10x2) mm yeng dilekati mesh ukuran (10x8x1) mm. Subyek penelitian dibagi 2 kelompok: 10 subyek plat kobalt kromium dengan mesh dilekati resin akrilik (20x10x2) mm dan 10 subyek penelitian plat kobalt kromium dengan mesh dietsa dengan akua regia 65% selama 5 menit, kemudian dilekati dengan resin akrilik (20x10x2) mm. dilakukan uji kekuatan tarik menggunakan Torsee’s universal testing Machine dengan ukuran kg/mm2, kemudian hasil analisa dengan t-test. Hasil Penelitian menunjukkan rata-rata kekuatan tarik perlekatan bahan resin akrilik dengan kerangka logam yang tidak dietsa lebih kecil daripada yang dietsa kimia dengan akua regia. Terdapat perbedaan yang bermakna kekuatan tarik perlekatan resin akrilik dengan kerangka logam yang tidak dietsa dengan yang dietsa secara kimia dengan akua regia (p<0,05). Kesimpulan. Etsa kimia dengan akua regia pada kerangka logam, meningkatan perlekatan bahan resin akrilik pada gigi tiruan kerangka logam. Background. Materials used in making removable partial denture (RPD) can be acrylic resins, metal plate, combination between metal plate and acrylic resins. Acrylic resins is plate denture material have good colour and translucent but the mechanical characteristic is not ideal, unstable to the abrasion, and can be occurred change in dimension. The frame removable partial denture is strong enough but estethically unsatisfaying, therefore need the acrylic resins and metal plate combination RPD. The purpose of this study was to know the influence of chemical etching with akua regia towardbond strength of acrylic resins in frame denture. Methods. The study was conducted in 20 subject of chromium cobalt plate (10x10x2) mm that was attached with a mesh measurement (10x8x1) mm. The sample were devided into two groups. The first group consisted of 10 samples of chromium cobalt plate (10x8x2) mm with a mesh was attached with acrylic resins (20x10x2) mm. The second group consisted of 10 samples of chromium cobalt plate (10x10x2) mm with a mesh etched with 65% akua regia for 5 minutes, than attached with acrylic resins (20x10x2) mm. the bond strength test was carried out using Torsee’s Universal Testing Machine in kg/mm2, than was statistically analyzed using t-test. The result of this study demonstrated that mean of bond strength of acrylic resins combined with nonetched metal plate was smaller than that with etched metal plate. There was a significant difference of the bond strength of acrylic resins combined with metal plate etched and that with metal plate non-etched (p<0,05). Conclusion. The chemical with akua regia in the metal frame increases the attachment of the acrylic resin in the frame denture.

Protesa Maksilofasial Thermoplastic Nylon (Valplast) dengan Hollow Bulb (Klas III Aramany palate schisis hereditary) A. Azhindra; Haryo Mustiko Dipoyono; Titik Ismiyati
Majalah Kedokteran Gigi Indonesia Vol 19, No 1 (2012): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Latar Belakang: pada penderita palato schisis (celah langit-langit)yang disebkan hereditary atau bawaan lahir terlihat defect yang menyebabkan gangguan bicara (sengau), penelanan, pengunyahan, estetik, dan psikologis. Untuk dapat mencapai fungsi bicara, fungsi mengunyah dan fungsi estetika diperlukan protesa untuk menutup celah tersebut. Tujuan: untuk meninformasikan cara rehabilitas defect atau cacat pada wajah dengan protesa maksilofasial thermoplastic nylon dengan hollow buib yang berguna untuk mengembalikan fungsi bicara, penelanan, pengunyahan, estetik dan psikologis penderita. Kasus dan penanganan: pasien pria berusia 46 tahun dating ke RSGM Prof. Soedomo atas rujukan dari poli RS. Dr. Sardjito. Saat datang pasien terganggu berbicara, menguyah dan menelan disebkan adanya celah langit-langit terbuka dan merupakan kelainan bawaan. Pasien kehilangan banyak gigi terutama pada gigi posterior pada rahang atas dan ingin dibuatkan gigi tiruan. Obturator ini dibuat segera dengan mempertimbangkan penutupan celah langit-langit, menggunakan bahan yang lebih ringan (menggunakan hoolow bulb) agar keluhan pasien dapat diatasi didesain alat yang mempunyai retensi maksimal dan mengembalikan pengunyahan, fungsi bicara, penelanan, estetis dan psikologis sehingga pasien akan akan mempunyai bentuk wajah yang mendekati normal. Hollow bulb adalah rongga yang dibuat pada protesa maksilofasial untuk menutup rongga mulut, rongga hidung dan defect. Pada waktu insersi diperiksa retensi, stabilisasi, oklusi, estetik dan pengucapan. Kontrol dilakukan 1 minggu dan 1 bulan setelah pemakaian. Hasil pemeriksaan dan evaluasi setelah 1 minggu dan 1 bulan setelah pemakaian protesa maksilofasial hollow bulb didapatkan hasil dengan retensi, stabilisasi, olusi dan pengucapan lebih baik. Kesimpulan: setelah menggunakan protesa maksilofasial thermoplastic nylon dengan hollow buib pada penderita palato scisis, pasien dapat berbicara dan mengunyah dengan normal. Protesa maksilofasial hollow bulb thermoplastic nylon juga dapat mengembalikan estetik yang maksimal sehingga pasien dapat menambah kepercayaan dirinya serta mengembalikan keadaan psikologi pasien yang telah lama menurun. Background: patients with palato schisis (clelf palate) due to hereditary or congenital defect will be seen that cause speech disorders (nasal), swallowing, mastication, esthetic and psychological. Purpose: to inform the way rehabilitation defect in the face with a maxillofacial prosthesis thermoplastic nylon with hollow bulb that is useful to restore the fuction of speech, swallowing, mastication, esthetics, and psychiatric patients. Case and handling: 46-yearold male patient came to RSGM Prof. Soedomo referral from Dr. Sardjito hospital. When patient come to feel annoyed talking, chewing ang swallowing due to the precence cleft palate is open and is a congential abnormality accompanied with loss of many teeth. Obturator is made immediately by considering the closure of cleft palate, using a lighter material (using a hollow bulb) with retention, stabilization and occlusion of the right and restore normal nendekati face shape. Hollow bulb is a cavity created in maxillofacial, prosthesis, to close the oral cavity, nasal cavity and the defect.at the time of insetation examined retention, stabilization, occlusion, esthetics and pronunciation. Control was performed 1 week and 1 month after application. The results of the examination and evaluation after 1 week and 1 month after the use of hollow bulb know maxillofacial prostheses retentation, stabilization, occlusion and better pronunciation. Conclusion: maxillofacial prostheses after using thermoplastic nylon with hollow bulb in patients with palate scisis, patients can speak and chew normaly. Maxillofacial prostheses nylon thermoplastic hollow bulb can also restore the maximum aesthetic, especially in the lose of anterior teeth with retention or grip that can mimic the gingival so the patient increase self confidence and restore the patient’s psychological state that has longbeen declined.

Pembuatan Gigi Tiruan Lengkap Obturator Rahang atas pada Kasus Kelas III Arammany dengan Penguat Mini Dental Implant untuk Protesa Rahang Bawah F. Wihan Pradana; Haryo Mustiko Dipoyono; Titik Ismiyati
Majalah Kedokteran Gigi Indonesia Vol 18, No 1 (2011): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Latar belakang. Kasus Edentulous dengan defek maksilofasial pada geriatri merupakan kasus yang komplek dan menyangkut estetika serta fungsi rongga mulut. Penggunaan hollow bulb dan implant untuk memberikan retensi merupakan alternatif cara yang dapat digunakan dalam perawatan defek maksilofasial dan sisa lingir alveolar yang tipis. Tujuan. dari pembuatan protesa GTL ini ialah untuk mengembalikan fungsi pencernaan rongga mulut sehingga dapat mengembalikan kemampuan mencerna dan mengolah makanan. Pembuatan GTL diupayakan untuk dapat retentif dan stabil sehingga protesa dapat bermanfaat sebagai instrumen rehabilitatif dalam rongga mulut. Kasus. Pasien laki-Iaki usia 70 tahun datang ke klinik prostodonsia FKG UGM dengan keluhan gigi tiruan lengkap yang longgar dan tidak dapat berfungsi dengan baik sehingga makanan dan minuman dapat keluar dari hidung melalui lubang di area palatum yang timbul kurang lebih 2 bulan sebelum datang ke RSGM. Penanganan. Setelah dilakukan proses bedah pengangkatan tumor oleh Onkologis selanjutnya dilakukan perencanaan pembuatan GTL. Paska operasi pasien mengenakan GTL sementara dari GTL lama pasien yang diperbaiki. Proses selanjutnya ialah pembuatan GTL baru untuk mengganti GTL lama yang rusak. Setelah GTL selesai dibuat, dilakukan insersi dan dilanjutkan dengan pemasangan mini dental implant untuk rahang bawah. Satu minggu berikutnya dilakukan pemasangan metal housing implant pada fitting surface gigi tiruan lengkap rahang bawah sehingga implant dan gigi tiruan menjadi satu kesatuan gigi tiruan lengkap dengan dukungan implant. Kesimpulan. Kontrol2 minggu dan satu bulan menunjukan implant masih stabil dan tidak ada keluhan pada pasien. Pasien dapat menggunakan gigi tiruannya untuk makan dan minum. Dari pemeriksaan subjektif diketanui bahwa dan makanan ataupun minuman tidak lagi keluar melalui hidung pasien.

Pengaruh Penambahan Fluor pada Resin Akrilik terhadap Kekerasan Basis Gigi Tiruan Sebagian Titik Ismiyati; Suparyono Saleh
Majalah Kedokteran Gigi Indonesia Vol 18, No 1 (2011): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Latar Belakang. Penelitian ini adalah salah satu efek pemakaian gigi tiruan sebagian adalah mudah terjadinya karies pada gigi yang masih tinggal. Hal ini karena kecenderungan terbentuknya plak pada permukaan gigi dan abrasi karena klamer pada gigi penyangga. Usaha untuk mencegah karies gigi adalah melakukan tindakan fluoridasi. Macam fluor yang sering digunakan adalah sodium fluorida 2,27% (NaF2). Bahan yang sering digunakan untuk membuat plat dasar gigi tiruan sebagian adalah resin akrilik yang komponennya mengandung polimetil metakrilat. Tujuan. Penulisan ini untuk mengetahui fluoridasi melalui gigi tiruan yaitu dengan meneliti penambahan fluor pada resin akrilik terhadap sifat kekerasan basis gigi tiruan sebagian. Metoda. Subyek penelitian berupa 40 plat resin akrilik kuring panas dan kuring dingin yang dicampur dengan sodium fluorida (NaF2), ukuran 20 x 15 x 2,5 mm. Pencampuran sodium fluorida dan resin akrilik dengan perbandil'lgan 1 : 3. Pada kelompok 1 resin akrilik kuring panas dicampur dengan sodium fluorida, kelompok II akrilik resin kuring dingin dicampur dengan sodium fluorida, kelompok 3 resin akrilik kuring panas tanpa penambahan sodium fluorida, dan kelompok 4 resin akrilik kuring dingin tanpa penambahan fluor. Uji kekerasan dilakukan dengan menggunakan alat Micro Vickers Hardness Tester. Data yang diperoleh dianalisis dengan menggunakan statistik anava 2 jalur. Hasil penelitian. Menunjukkan bahwa terdapat perbedaan yang bermakna kekerasan resin akrilik kuring panas dan resin akrilik kuring dingin (p 0,05). Kesimpulan. Penelitian ini adalah penambahan sodium fluorida pada resin akrilik kuring panas dan pada resin akrilik kuring dingin tidak mempengaruhi kekerasan basis gigi tiruan sebagian.

Campuran kitosan dengan resin akrilik sebagai bahan gigi tiruan penghambat Candida albicans Titik Ismiyati; Widowati Siswomihardjo; Marsetyawan Heparis Nur Ekandaru Soesatyo; R. Rochmadi
Majalah Kedokteran Gigi Indonesia Vol 3, No 3 (2017): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

The mixture of acrylic resin and chitosan as denture material to inhibit Candida albicans. The inhibition of Candida albicans in denture resin has an important role to prevent the development of denture stomatitis. Chitosan is a natural polymer compound derived from shrimp waste which can function as an antifungal Acrylik resin cannot be mixed with chitosan. To obtain a homogeneous mixture, the mixture was added a coupling agen acrylic acid and acetone. The research objective was to study the mixture of acrylic resin and chitosan with solvent acrylic acid and acetone as a denture that can inhibit the growth of Candida albicans. Methods: The samples used discs in 10 mm diameter and 2 mm thickness, made from heat cured acrylic resin mixed with chitosan dissolved in acrylat acid and acetone. They were divided into 4 groups. Group 1 was acrylic resin without chitosan as a control, group 2, 3 and 4 were the mixture of acrylic resin and 5 ml chitosan in 0.5%, 1%, and 2% concentration respectively. The fourier transform irfrared spectroscopy (FTIR) and the digital optical microscope were used to synthesize and analyze. The Kruskal Wallis was used to analyze the data. The results showed that the mixture of acrylic resin with chitosan significantly inhibited the growth of Candida albicans. Conclusion: a mixture of acrylic resins and chitosan can be fungistatic, so it can be developed as an antifungal denture material. ABSTRAKPenghambatan Candida albicans pada gigi tiruan resin akrilik dapat memainkan peran penting dalam mencegah perkembangan denture stomatitis. Kitosan adalah senyawa polimer alam yang berasal dari limbah udang yang dapat berfungsi sebagai antijamur. Resin akrilik tidak dapat bercampur dengan kitosan. Untuk mendapatkan campuran yang homogen, campuran tersebut ditambah coupling agent asam akrilat dan aseton. Penelitian ini bertujuan untuk mengkaji campuran resin akrilik dan kitosan dan asam akrilat pelarut aseton sebagai bahan gigi tiruan penghambat Candida albicans. Spesimen penelitian berbentuk cakram berdiameter 10 mm dan tebal 2 mm dibuat dari resin akrilik kuring panas (QC 20) dicampur dengan kitosan dari cangkang udang yang ditambahkan asam akrilat dalam pelarut aseton. Spesimen dibagi menjadi 4 kelompok. Kelompok 1 terdiri dari resin akrilik tanpa kitosan sebagai kontrol, sedangkan kelompok 2, 3, dan 4 terdiri dari campuran resin akrilik dengan kitosan 5 ml pada konsentrasi 0,5%, 1%, dan 2%, secara berurutan. Hasil campuran resin akrilik dengan kitosan dianalisis dengan menggunakan Fourier Transform Infrared spectroscopy (FTIR), dan digital mikroskop optik. Efek antijamur diuji dengan menggunakan metode dilusi. Data yang didapat dianalisis statistik dengan Kruskal Wallis. Hasil penelitian menunjukkan bahwa campuran resin akrilik dengan kitosan signifikan menghambat pertumbuhan Candida albicans. Berdasarkan hasil yang diperoleh, dapat disimpulkan bahwa campuran resin akrilik dan kitosan dapat bersifat fungistatik, sehingga dapat dikembangkan sebagai bahan gigi tiruan antijamur.

Effect of nano silica coating concentration on reducing acrylic resin denture cytotoxicity Gene Rizky Natalia Gunawan; Titik Ismiyati; Haryo Mustiko Dipoyono
Majalah Kedokteran Gigi Indonesia Vol 5, No 3 (2019): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.37166

Acrylic resin is a denture base material mostly used in the fabrication of removable partial denture and complete denture because it is easy to process, economical and aesthetically satisfying. However, the acrylic resin has a weakness i.e. residual monomer content which causes cytotoxic effect to human cells. Nano-silica coating could be used on the acrylic resin to reduce residual monomer content. The purpose of this study was to determine the effect of nano-silica coating concentration on the cytotoxicity of acrylic resin denture base material. Twenty-four acrylic resin disc-shaped specimens (5 mm in diameter and 2 mm in thickness) were divided into 4 groups (n=6), i.e. control, group with application of 0.5%,1%, and 2% nano silica coating. The specimens on the silica coating groups were silica coated using dip-coating method. After the coating was completed, the specimens were added into cell fibroblast culture (vero cell line). Cytotoxicity testswere done by MTT assay. The optical density was measured using ELISA plate reader to determine the percentage of cell viability. The highest mean of cell viability was shown in 0.5% concentration (96.78 ± 1.71) and the lowest mean was shown in the control group (80.32 ± 6.53). One-way ANOVA showed that there were significant differences among these groups. LSD tests showed that there was significant difference among all of the groups. This study concluded that nano silica coating concentration has a significant effect on the cytotoxicity of acrylic resin denture base materials.

Effect of addition titanium dioxide nanoparticles as acrylic resin denture base filler on cytotoxicity Ardhianing Hardita; Titik Ismiyati; Endang Wahyuningtyas
Majalah Kedokteran Gigi Indonesia Vol 5, No 2 (2019): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.38438

Denture base material should have a good level of biocompatibility. Acrylic resin is frequently used as a denture base material, however it has a disadvantage of producing residual monomer. Residual monomer is known to have a cytotoxicity effect. Titanium dioxide (TiO2) nanoparticles are used as fillers due to their biocompatibility and ability to enhance the mechanical properties of acrylic resin. The addition of the material to acrylic resin could affect the amount of residual monomer. The aim of this study was to examine the effect of the addition of TiO2 nanoparticles as acrylic resin denture base filler on the cytotoxicity in fibroblast cells. The samples consisted of 24 heat cured acrylic resins in disc shape (5 mm in diameter and 2 mm in thickness), divided into 4 groups (n = 6): three groups given treatment with0.5%, 1%, 2% TiO2, respectively and one control group. Cell viability was measured with MTT assay. The results were tested with one way ANOVA with 95% confidence level followed by LSD post hoc test. The results showed that the highest percentage of cell viability was found in the treatment group of 0.5% TiO2 with value of 91.83 ± 1.75%, while the lowest value was seen in the treatment group of 2% TiO2 with value of 79.38 ± 3.34%. Significant differences were shown between the treatment groups of 0.5% and 2% TiO2, as well as between the control and treatment group with 2% TiO2. The conclusions of this research are the addition of TiO2 nanoparticles as acrylic resin denture base filler has an effect on cytotoxicity; the addition of 0.5% TiO2 nanoparticles filler results in lower cytotoxicity on fibroblast cells compared to the addition of 1% and 2% TiO2.

The effect of paint types toward iris color changes of ocular prosthetics Gunawan Sri Sarjono; Titik Ismiyati; Endang Wahyuningtyas
Majalah Kedokteran Gigi Indonesia Vol 6, No 1 (2020): April
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.38706

Ocular prosthetics are mainly created to meet the aspect of esthetic consideration. The paint application in ocular prosthetics construction is remaining an option for Prosthodontist. This study aims to examine the effect of types of paint toward iris color change of the ocular prosthetics. Three black types of paint: oil paint (Maries Oil Color, China), automotive paint (Avian, Indonesia), and acrylic paint (Sakura, Japan) were used to paint paper disc to produce 27 iris ocular prosthetics. Chromameter was used to examine the first and the final color after acrylic processing using themicrowave. The measurement results in the process using Commission Internationale de l’Eclairage. One-way ANOVA test showed significant differences between the three types of paints on ocular prosthetics (p<0.05). The LSD test revealed that oil paint groups had more significant results than the automotive paint group and acrylic paint group, while the automotive paint group did not have significant differences with the acrylic paint group (p>0.05). Thus, it is conclusive that oil paint influences the iris color change of the ocular prosthetics.

The effect of Zirconium Dioxide nanoparticles concentrations as filler on heat cured acrylic resin denture base toward viability of fibroblast cells (in vitro study) Rudy S; Titik Ismiyati; Endang Wahyuningtyas
Majalah Kedokteran Gigi Indonesia Vol 6, No 2 (2020): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.44345

Heat cured acrylic resin is the most commonly used denture base materials. Zirconium dioxide (ZrO2) nanoparticles can be applied as additional filler to increase mechanical strength and to reduce the amount of residual monomer. The aim of this research is to analyze the effect of ZrO2 nanoparticles concentrations as filler on heat cured acrylic resin denture base toward viability of fibroblast cells. Twenty four disc-shaped heat cured acrylic resin plate (diameter 5 mm; width 2 mm), were divided into 4 groups (n=6), they were consisted of group I control (acrylic resin), group II acrylic resin with 2.5% ZrO2, group III acrylic resin with 5% ZrO2, and group IV acrylic resin with 7.5% ZrO2. Cell viability was obtained using MTT assay and ELISA plate reader. The result is examined with one way ANOVA followed by LSD post hoc assessment. The result showed highest cell viability percentage on experimental group of 2.5% ZrO2 with value as high as 97.49%. One way ANOVA test and LSD post hoc test showed a significant difference between groups (p<0.05). The conclusion of this research is ZrO2 nanoparticles concentration utilized as filler on heat cured acrylic resin denture base is effect to viability of fibroblast cells, and ZrO2 nanoparticles 2.5% shows the highest viability of fibroblast cell compared to 5% and 7.5% ZrO2 nanoparticles concentrations

UJI SITOKSISITAS CAMPURAN RESIN AKRILIK DENGAN KITOSAN SEBAGAI BAHAN GIGI TIRUAN ANTI JAMUR Titik Ismiyati; Widowati Siswomihardjo; Marsetyawan H.N.E. Soesatyo; Rochmadi Rochmadi
Jurnal Teknosains Vol 5, No 2 (2016): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.27580

Denture acrylic resin contain monomer residue that can cause allergic reactions and inflammation in the mouth. Chitosan has advantage biocompatible and antifungal. The purpose of this study was to examine toxicity acrylic resin blends with chitosan as denture antifungal in fibroblast cell culture. This study uses chitosan concentration 0.5%, 1%, 2% and 4% of 2.5 ml were blends with acrylic resin. Fibroblast cell cellular responses were assessed using MTT assay. Data at the cell viability analyzes used Anova one path (p <0.05). The results showed the greatest average adsorbansi fibroblast cell in blends acrylic resin was chitosan concentration of 0.5% (0.434 ± 0.119) with 99.810% cell viability, and the smallest average chitosan concentration of 4% (0.385 ± 0.023) and 88.523% cell viability. Anova test showed there were differences the effect of varying concentrations of chitosan significantly to adsorbansi and cell viability (p <0.05). The results of post hoc test showed a concentration of 4% was significantly different than other concentration. Conclusion, acrylic resin blends with chitosan at a concentration of 0.5%, 1%, 2% were non-toxic, being mild toxic concentration of 4%.

Co-Authors A. Azhindra Abdullah, Johari Yap Adella Syvia Maharani Alma’ruf, Fattah Anak Agung Istri Putri Ananto Ali Alhasyimi Angeline Antony Arie Sigit Nuryanto Arief Waskitho Ayu Rahayu Christine Anita Wardaningrum Endang Wahyuningtyas Erriza, Vania Erwan Sugiatno Erwan Sugiatno Erwan Sugiatno Erwan Sugiatno Erwan Sugiatno, Erwan Esti Tjahjanti F. Wihan Pradana Gene Rizky Natalia Gunawan Gene Rizky Natalia Gunawan Gunawan Sri Sarjono Hardita, Ardhianing Haris, Amnan Haryo Mustiko Dipoyono Haryo Mustiko Dipoyono Haryo Mustiko Dipoyono Haryo Mustiko Dipoyono Haryo Mustiko Dipoyono Haryo Mustiko Dipoyono Haryo Mustiko Dipoyono Haryo Mustiko Dipoyono, Haryo Mustiko Helmi Fathurrahman, Helmi Herijanti Amalia Kusuma Heriyanti Amalia Kusuma Heriyanti Amalia Kusuma Huri, Stevani Gracela I Gede Made Hadi Nugraha Arisukra I Gede Putu Sukrasena Sugiantara Ihwanul Aziz Intan Ruspita Intan Ruspita, Intan Kevin Christopher Kawilarang M Th Esti Tjahjanti Maria Theresia Esti Tjahjanti Marsetyawan H.N.E. Soesatyo Murti Indrastuti Murti Indrastuti Murti Indrastuti Najwa, Rizqa Naufal, Muhammad Agnaf novita sari, dian Ollive, Gabriella Maria Pradama, Daffa Evan Pramudya Aditama Pramudya Aditama Putri Dhianita Pratiwi R. Rochmadi Revalina, Aurelia Dias Nanda Rizki Nor Amelia Rochmadi Rochmadi Rosa Sharon Suhono Rosa Sharon Suhono, Rosa Sharon Rudy S Rudy S Sarjono, Gunawan Sri Soesatyo, Marsetyawan Heparis Nur Ekandaru Sradha Putra Sri Budi Barunawati Suparyono Saleh Suparyono Saleh Suzy Ratna Dinarti Trida Ridho, Fariz Widowati Siswomihardjo Wijaya, Wina M. Windriyatna Windriyatna

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