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All Journal HAYATI Journal of Biosciences International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Bumi Lestari Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology SIMBIOSIS JURNAL HUBUNGAN INTERNASIONAL Folia Medica Indonesiana Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Bawal : Widya Riset Perikanan Tangkap

Ngurah Intan Wiratmini

Program Studi Biologi Fakultas MIPA Universitas Udayana, Bali

Author-ID : 287691

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Public Health Other

Published : 36 Documents Claim Missing Document

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STRUKTUR HISTOLOGI HATI MENCIT(Mus musculus L.) SETELAH PEMBERIAN EKSTRAK DAUN EKOR NAGA (Rhapidhophora pinnata Schott) Ni Komang Tia Pramesti; Ngurah Intan Wiratmini; Ni Putu AdrianiAstiti
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

ABSTRACT Rhapidhophora pinnata, Schott is a traditional medical plant that has been use remedy for treatment of hypertension, stroke. Aims of this research is to determine the effect of Rhapidhophora pinnata Schott leaf extract toward mouse (Mus musculus L) liverhistological structure. This research used completely randomized design with 28 female mice that were divide into 4 groups of P0 (control) received 0,9% NaCl, group P1, P2, and P3 received 50, 100 and 150 mg/kg bw respectively by oral administration. The treatment were given daily for 14 days. All of the mice were sacrificed by ether after 15 days. The liver were examined for their histopathological changes, namely fatty degeneration, hydropic degenaration and necrosis. Results were statistically analised by Kruskal-Wallis method. Treatment groups showed no significant differences with regard of liver histopatological changes, however hemorrhage, sinusoid congestion and inflamatory cell infiltration were found in liver. Keyword : Rhapidhophora pinnata Schott, liver, mice

EFEKTIVITAS EKSTRAK DAUN SUKUN (Artocarpus communis Forst.) DALAM MENURUNKAN KADAR GLUKOSA DARAH DAN MEMPERTAHANKAN JUMLAH SPERMA PADA TIKUS (Rattus norvegicus L.) I Putu Ari Wijana Dipa; Ni Wayan Sudatri; Ngurah Intan Wiratmini
SIMBIOSIS Vol 3 No 2 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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Breadfruit (Artocarpus communis Forst.) leaves have a high antioxidant content. This research aims to know the effect of breadfruit leaf extracts on blood sugar levels and the amount of sperm in rat. This study used a Completely Randomized Design (CRD) using 30 rats aged 2-3 months and weighing 150-200 grams. Male rats were divided into six groups, i.e. negative control group (K1) without treatment, groups K2, P1, P2, P3 and P4 are given then NaCl 0.9%, breadfruit leaf extracts 50 mg/kg, 100 mg/kg, 200 mg/kg body weight, and glibenclamide 1 mg/kg body weight respectively. The treatments were given every day for 21 days. Blood sugar levels were measured before and after aloksan administration, day 7, 14, and 21 after treatment as well as counting the number of sperm after 21 days. The data obtained were analyzed with ANOVA and Duncan Multiple Range Test (DMRT), using the program of SPSS For Windows version 22. Results of the study showed that breadfruit (Artocarpus communis Forst.) leaf extracts could lowered blood glucose levels and the most effective concentration was 100 mg/kg body weight of breadfruit leaf extracts given for 21 days with the effectiveness of 66,77%. Breadfruit leaf extracts did not inhibit spermatogenesis. So the amount of rat sperm did not different significantly among treatment. Keywords: blood sugar levels, breadfruit, rat, sperm count

HISTOLOGI HATI MENCIT (Mus musculus L.) YANG DIBERI EKSTRAK DAUN LAMTORO (Leucaena leucocephala) I Wayan Andi Yoga Kurniawan; Ngurah Intan Wiratmini; Ni Wayan Sudatri
SIMBIOSIS Vol 2 No 2 (2014)
Publisher : Jurusan Biologi Universitas Udayana

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Leucaena (Leucaena leucocephala) has been used as traditional medicine, such asdrugs to decrease the level of blood glucose, so it can be used as drug to indicatediabetes. The aim of this research is to study the histopathological change of mice’s livergiven leucaena leaf extract. This research used 24 male mice that were divided into 4 groupsby completely randomized design. Group P0 (control) were given 0,9 % NaCl, and groupsP1, P2, and P3 were given 0,5 g / kg bw, 1 g / kg bw, and 1,5 g / kg bw leucaena’s leafextract by oral administration respectively. The treatment were given daily for 30 days. All ofthe mice were necropsied at day 31, and the liver were taken to examine theirhistopathological change. Histopathological change examination were based on the presenthydropic degeneration, fatty degeneration, and apoptosis surround central vein. Result ofstatistically analised by ANOVA and Kruskal-Wallis method indicated no significancedifference among the control and treatments. There was no significance toxicity effect ofleucaena leaf extract of all treatment dosage on mice liver.Keywords: Leucaena leucocephala, liver histology, Mus musculus L.

Histological Structures of Gills of Tilaphia Fish (Oreochromis mossambicus L.) As A Water Quality Indicator In The Nusa Dua Sewage Tretment Ponds, Bali Wahyu Puji Lestari; Ngurah Intan Wiratmini; A. A. Gde Raka Dalem
SIMBIOSIS Vol 6 No 2 (2018)
Publisher : Jurusan Biologi Universitas Udayana

Nusa Dua is a popular tourist resort in Bali, where many hotels and other tourist facilities were built. In this resort, the sewage is centralized processed in a Sewage Treatment Ponds called “Unit Pengolahan Air Limbah PT ITDC” which is also often called Lagoon Nusa Dua. Nusa Dua sewage treatment ponds have 5 units of ponds, namely Pond 1A, 1B, 2A, 2B, and Pond 3. In ponds 2B and 3, tilapia fish (Oreochromis mossambicus Linn.) was raised as a water quality bio-indicator. The aim of this research was to determine the histology condition of the gill of tilapia fish that raised in the Nusa Dua sewage tretment ponds which were then used as an indicator of water quality in these ponds. The research employed a complete randomized design in which 16 samples were taken from two sampling sites with eight replications. Results of this research showed that histologic changes of the gill that were observed including edemas, fusions, and hyperplasias of secondary lamellas as well as telangiectases. Keywords: Nusa Dua sewage, Oreochromis mossambicus L., gills, histology, bioindicator

Biokimia Darah Tikus (Rattus norvegicus) Betina Ovariektomi setelah Perlakuan Ekstrak Daun Kaliandra Merah (Calliandra calothyrsus Meissn.) Iriani Setyawati; Ngurah Intan Wiratmini; Ni Putu Ayu Dewi Wijayanti
SIMBIOSIS Vol 7 No 1 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Red calliandra (Calliandra calothyrsus Meissn.) leaf have the potential as phytoestrogens. In this study, adult female ovariectomized rats (as a model of menopause women) were given calliandra leaf extract containing estrogenic flavonoids. This study was an experimental design with a Completely Randomized Design (CRD). Forty-five ovariectomized female rats were divided into five groups i.e. untreated control (K), and the treatment group given 80% of C. calothyrsus ethanolic leaves extract doses 25 mg/ kg bw (P1), 50 mg/ kg bw (P2), 75 mg/ kg bw (P3) and 100 mg/ kg bw (P4). The test suspension was given once a day at 24-hour intervals by gavage as much as 0.5 ml/rat/day for two months. At the end of the treatments, blood was collected for blood biochemical tests including hemoglobin levels, hematocrit values, blood glucose and proteins levels. The results showed that C. calothyrsus leaf extract did not negatively affect the health status of the animals through blood biochemical parameters, i.e. hemoglobin levels, hematocrit values, and glucose and protein levels of ovariectomized female rats.

LEAD (Pb) CONTENT AND STOMACH HISTOLOGICAL STRUCTURE IN TILAPIA (Oreochromis mossambicus) FISH ORGANS LIVED IN THE WATERS OF UPAL PT. ITDC shanon lestari; Ngurah Intan Wiratmini; Job Nico Subagio
SIMBIOSIS Vol 8 No 2 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Nusa Dua merupakan kawasan pariwisata dengan rancang bangun yang komprehensif dan terpadu sebagai sarana akomodasi wisata berkelas Internasional. Pengelolaan limbah cair dikelola secara terpadu pada Unit Pengelolaan Air Limbah (UPAL) milik PT. ITDC. Hotel – hotel membuang limbah cairnya melalui collection pit yang akan diakumulasikan di kolam UPAL. Tiga kolam terakhir digunakan sebagai kolam pemeliharaan ikan salah satunya ikan mujair, sebagai bioindikator. Ikan mujair dikolam itu dapat hidup namun belum diketahui bagaimana histologi dan anatomi organnya, sehingga perlu kajian histologis. Rancangan penelitian yang digunakan Rancangan Acak Lengkap dengan pengambilan sampel ikan mujair pada dua titik pengambilan dari area lagoon. Sampel ikan mujair diambil sebanyak 8 kali sebagai ulangan, dari setiap kolam yaitu kolam nomor 2 B dan kolam nomor 3. Variabel yang diamati adalah kandungan timbal (Pb) pada daging ikan dan kerusakan jaringan pada organ lambung . Rerata kandungan timbal dalam daging ikan dari kolam 2B sebesar 1,85 mg/kg, sedangkan dari kolam 3 sebesar 1,24 mg/kg. Jumlah sel dengan inti karyoreksis dan jaringan yang mengalami desquamasi sel epitel pada lambung ikan mujair (O. mossambicus) dari kolam 3 lebih rendah secara nyata dibandingkan di kolam 2B. Peralihan dari kolam 2B ke kolam 3 mengurangi kandungan timbal dan kerusakan histologis. Kata Kunci: mujair, lambung, logam berat, Pb

IDENTIFIKASI SENYAWA AKTIF DAUN PLETEKAN (Ruellia tuberosa L.) DENGAN MENGGUNAKAN GC-MS Ida Ayu Nopiari; Ni Putu Adriani Astiti; Ngurah Intan Wiratmini
SIMBIOSIS Vol 4 No 2 (2016)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (589.972 KB)

ABSTRACT The use of traditional medicines in human therapies has been claimed to be much safer than that of synthetic medicines with many negative side effects. Pletekan plant (Ruellia tuberosa L.) has been used empirically for years as an antidiabetic as its leaves contain poly phenolic compounds with the ability to stimulate beta cells of the pancreas so that its capability to produce insulin improved. The main objective of our research was to identify active compounds contained in Ruellia tuberosa L. leaves extracted with ethanol. Fractionation of these active compounds was conducted by applying column and thin layer chromatography using Dichlorometan and Ethylacetat with a ratio of 6:3 as eluent. Each active compound resulted from fractionation was identified using GC-MS analysis. The results showed that four active compounds (Hexadecanamide, 9-Octadecenamide, (Z), Octadecenamide and 1,2-Benzenedicarboxylic acid were identified in this analysis. Keywords: Ruellia tuberosa L., identification of active compounds.

Co-Authors A.A.S. A. Sukmaningsih AA Istri Mas Padmiswari Anak Agung Gde Raka Dalem Apriliani, Sinta Arnenda, Gussasta Levi Bram Setyadji Dessy Sukma Wirastuti Elfira Dzikri Ashfahani G.A.A Rina Febrina Gussasta Levi Arnenda I Gede Wiranatha I GUSTI AYU MANIK ERMAYANTI I Made Sara Wijana I Made Sara Wijana I Putu Ari Wijana Dipa I Wayan Andi Yoga Kurniawan I Wayan Kasa I.M.A.S. Wijaya Ida Ayu Nopiari Ida Ayu Ratih Purnama Dewi Inna Narayani IRIANI SETYAWATI Jaqueline Sudiman Job Nico Subagio Joko Raharjo, Sentot Joko Wiryatno Luh Putu Wrasiati MADE PHARMAWATI Maria Antonia Margaretha Ferandez Maria Antonia Margaretha Fernandez Mery Wintari Ni Desak Made Aprilia Dewi Ni Komang Tia Pramesti Ni Luh Eka Setiasih Ni Luh Suriani Ni Made Rai Suarni Ni Putu Adriani Astiti Ni Putu Ayu Dewi Wijayanti Ni Putu Sri Risa Dewi Ni Wayan Adya Puttry Purna Yogini Ni Wayan Sudatri Putu Titah Kawitri Resen Salsabillah Rahma SANG KETUT SUDIRGA shanon lestari Siti Masnunah Sri Sulastri Sukma Sushanti Wahyu Puji Lestari WAHYU WIDOWATI

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