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All Journal HAYATI Journal of Biosciences International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Bumi Lestari Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology JURNAL HUBUNGAN INTERNASIONAL Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Bawal : Widya Riset Perikanan Tangkap Folia Medica Indonesiana SIMBIOSIS Jurnal Biologi Udayana
Ngurah Intan Wiratmini
Program Studi Biologi Fakultas MIPA Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Neuroscience Public Health

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Journal : METAMORFOSA Journal of Biological Sciences

 1 
Uji Efektivitas Vitamin C sebagai Neuroprotektor pada Mencit (Mus musculus) yang Diinduksi Akrilamida Salsabillah Rahma; Ngurah Intan Wiratmini; Ni Wayan Sudatri
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p08

Abstract

Acrylamide is a contaminant compound found in several types of food containing carbohydrates and processed food through frying or roasting at high temperatures. Acrylamide compounds could cause the formation of oxidative stress which could lead to neurotoxicity. This oxidative stress could be minimized by administering the antioxidant vitamin C. This study was conducted to determine the mice cerebral cortex histology, the mice cerebellum histology induced by acrylamide and vitamin C supplementation, and the most effective dose as a neuroprotector. This study used a completely randomized design (CRD) with 4 groups, namely K + (acrylamide 12.5 mg / kg BW); K- (NaCl 0.9%); P1 (vitamin C dose of 0.26 mg/animal /day + acrylamide dose of 12.5 mg/kg BW); and P2 ((vitamin C dose of 0.52 mg/animal/day + acrylamide dose of 12.5 mg / kg BW) and 6 repetitions so that the number of mice used was 24. The acrylamide and vitamin C solutions were administered orally, and acrylamide given for 20 days, while vitamin C was given 3 days before acrylamide induction until day 20. Mice were dissected at day 21, brain organs were collected for histological preparations by the paraffin method and Hematoxylin-Eosin staining. The qualitative data obtained were analyzed by One Way ANOVA and continued with the Duncan test. The parameters observed were necrosis in pyramid cells and glial cells. The results showed that vitamin C at dose of 0.52 mg/animal/day significantly repaired the damage in pyramid cells and glial cells induced by acrylamide.
Keragaman Genetik dan Hubungan Kekerabatan Ayam Lokal Berdasarkan Penanda RAPD Mery Wintari; Made Pharmawati; Ngurah Intan Wiratmini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p13

Abstract

Indonesian local chicken is one kind of germplasm that has been utilized by the community for various needs, such as consumption, ceremonial, collection, and cockfighting. To maintain its sustainability, an inventory of genetic diversity can be made using molecular markers. This study aimed to determine the genetic diversity and relationship between four Indonesian local chickens: ayam kampung, ayam ketawa, ayam kate lokal, and ayam bekisar kangean, based on RAPD marker. DNA was extracted from blood samples of 12 local chickens consisting of 3 ayam kampung, 3 ayam ketawa, 3 ayam kate lokal, and 3 ayam bekisar kangean using Genetic DNA Mini Kit (Tissue) Geneaid. The PCR-RAPD analysis was performed using 6 primers, OPA 04, OPB 08, OPB 12, OPC 16, OPH 01, and OPH 03. The product of PCR-RAPD was electrophoresed using 1,5% agarose in 1x TAE buffer. The results showed that the similarity value of 12 local chickens using RAPD ranged from 0% to 100%. The relationship between 12 local chickens using RAPD marker indicated that the chickens in the same morphology were not clustered in the same group. This suggests that the same group of chickens phenotypically does not necessarily have the same genotype. Keywords: Local Chicken, PCR-RAPD
Kadar Hormon Estrogen Dan Ketebalan Endometrium Tikus (Rattus norvegicus) Betina Ovariektomi Setelah Pemberian Ekstrak Daun Lamtoro (Leucaena leucocephala Lam. De Wit) Maria Antonia Margaretha Fernandez; Ngurah Intan Wiratmini; Ni Made Rai Suarni
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p15

Abstract

Lamtoro (Leucaena leucocephala Lam. De Wit) merupakan tanaman yang digunakan sebagai obat dan pakan ternak. Lamtoro mengandung zat aktif dari golongan senyawa streroid, alkaloid, flavonoid, polifenol dan tannin. Zat aktif yang terkandung pada lamtoro bersifat fitoestrogen karena mengandung golongan senyawa steroid, alkaloid dan flavonoid. Tujuan penelitian ini untuk mengetahui pengaruh pemberian ekstrak daun lamtoro terhadap kadar hormon estrogen dan ketebalan endometrium tikus (Rattus norvegicus) betina yang telah diovariektomi. Penelitian ini menggunakan Rancangan Acak Lengkap dengan empat perlakuan yaitu K- (kontrol negatif diberi minyak jagung), K+ (kontrol positif diberi estrogen sintetik 2 mg), P1dan P2 diberi diberi ekstrak daun lamtoro sebanyak 200 mg/kg BB dan 250 mg/kg BB). Masing masing perlakuan terdiri dari 6 ulangan sehingga digunakan 24 ekor tikus betina. Ekstrak daun lamtoro diberikan secara oral sebanyak 2 ml/ekor/hari selama 15 hari. Data yang diperoleh dianalisis dengan One Way ANOVA dan dilanjutkan dengan uji Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan pemberian ekstrak daun Lamtoro (Leucaena leucocephala Lam. De Wit) dosis 250 mg/kg BB dapat meningkatkan kadar hormon estrogen serum darah dan ketebalan endometrium tikus betina yang diovariektomi. Kata kunci: Leucaena, Ovariektomi, Estrogen, Endometrium, Tikus
Histopatologi serta Aktivitas Hati Kelinci Lokal (Lepus sp.) yang Diberi Ransum Tepung Daun Kaliandra (Calliandra calothyrsus Meissn.) dan Kulit Nanas (Ananas comosus L.) I Gede Wiranatha; Iriani Setyawati; Ngurah Intan Wiratmini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p07

Abstract

The aimed of this study was to determine the effect of feed containing various concentrations of Calliandra calothyrsus Meissn. leaf meal mixed with pineapple peels and concentrate feed on histopathology and the activity of liver of local rabbit (Lepus sp). This study used a Post-Test Only Control Group Design, using 24 local male rabbits divided into 4 groups of feed treatment namely P0 = concentrate feed as control; P1 = mixture of 15% of calliandra leaf meal (CLM), 30% of pineapple peels (PP) and 55% of commercial feed (CF); P2 = 30% of CLM, 30% of PP and 40% of CF; and P3 = 45% of CLM, 30% of PP and 25% of CF). The treatments were given for 60 days and used 6 replicates for each group. The data were analyzed statistically by SPSS program version 22. The research results showed the increase of calliandra leaf meal levels in concentrated feed which were added 30% of pineapple peels still caused liver damage namely fatty degeneration, necrosis and congestion. It increased the ALT level, but did not affect the AST level in local rabbit serum.
UJI AKTIVITAS EKSTRAK BIJI KAPUK RANDU (Ceiba pentandra Gaertn.) TERHADAP PERILAKU KAWIN TIKUS (Rattus norvegicus) JANTAN Dessy Sukma Wirastuti; Ngurah Intan Wiratmini; Sang Ketut Sudirga
Metamorfosa: Journal of Biological Sciences Vol 5 No 1 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i01.p02

Abstract

Kapuk randu (Ceiba pentandra Gaertn.) merupakan salah satu tanaman yang memiliki banyak manfaat untuk masyarakat. Tanaman ini dimanfaatkan sebagai penahan tanah dari erosi, tanaman peneduh, bahan pengisi kasur dan pakan ternak. Saat ini biji kapuk randu banyak dikembangkan sebagai bahan obat kontrasepsi herbal. Tujuan penelitian ini adalah untuk mengetahui aktivitas ekstrak biji kapuk randu (Ceiba pentandra Gaertn.) terhadap perilaku kawin tikus (Rattus norvegicus) jantan. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 4 kelompok perlakuan dan 8 ulangan yaitu P0 (aquades dan tween 80), P1 (0,07 mg/kg BB ekstrak biji kapuk randu), P2 (0,12 mg/kg BB ekstrak biji kapuk randu), P3(0,24 mg/kg BB ekstrak biji kapuk randu). Pemberian perlakuan ekstrak biji kapuk dilakukan selama 33 hari dengan pengamatan perilaku kawin dilakukan 3 hari sekali. Parameter yang diamati untuk menilai adanya perilaku kawin adalah jumlah kissing vagina, jumlah mount jumlah intromission. Hasil penelitian menunjukkan bahwa ekstrak biji kapuk randu memberikan pengaruh yang nyata terhadap perilaku kawin tikus jantan yang ditunjukkan dengan menurunnya jumlah kissing vagina, jumlah mount dan jumlah intromission tikus jantan kelompok perlakuan.
Gambaran Histologi Lambung Dan Duodenum Mencit (Mus musculus L.) Jantan Yang Diberi Ekstrak Daun Kersen (Muntingia calabura L.) Setelah Diinduksi Monosodium Glutamat (MSG) Ni Wayan Adya Puttry Purna Yogini; Ngurah Intan Wiratmini; Ni Gusti Ayu Manik Ermayanti
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p02

Abstract

The antioxidant contained in kersen (Muntingia calabura L.) leaves has been known to be able to prevent animal tissue damages. Monosodium glutamate (MSG) is a food additive substance associated with histological damage of duodenum as well as increases secretion of HCl in stomach if consumed in excess amount. This study aimed to investigate the histological features of stomach and duodenum of monosodium glutamate-induced mice (M. musculus) after treated with kersen leaves extract, as well as establishing its optimum dose. This study used a Completely Randomized Design, with 25 male mice assigned to 5 treatment groups: 2 control groups i.e. negative control (K-) given aquades and positive control (K+) given 0.7mg/body weight MSG; and 3 intervention groups which were given kersen leaves extract as much as 0.075 mg/g bw (P1), 0.15 mg/g bw (P2) and 0.3 mg/g bw (P3). Both kersen leaves extract and MSG was administered orally once daily for 28 days. The parameters observed were histological features of stomach features (erosion of villi and inflammatory cells infiltration) and duodenum (erosion of villi, necrosis, and inflammatory cell infiltration). The results showed that all parameters observed in the aforementioned organs were significantly different (P<0.05) in intervention groups (P1, P2, dan P3) compared with positive control (K+). It is concluded that administration of kersen leaves extract improved the damages of stomach, duodenum, and liver of MSG-induced mice, and was best achieved at the dose of 0.3 mg/g bw.
Uji Efektivitas Neuroprotektif Ekstrak Daun Pepaya (Carica papaya L.) Terhadap Sel Piramidal Di Hipokampus Dan Korteks Serebri Mencit (Mus musculus L.) Yang Diinduksi Trimetiltin Siti Masnunah; Ngurah Intan Wiratmini; Ni Made Rai Suarni
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p05

Abstract

Penelitian ini bertujuan untuk mengetahui efek neuroprotektif ekstrak daun pepaya (Carica papaya L.) terhadap sel piramidal di hipokampus dan korteks serebri mencit (Mus musculus L.) yang diinduksi Trimetiltin serta untuk mengetahui dosis optimum ekstrak daun pepaya yang bersifat neuroprotektif. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan lima perlakuan yang terdiri dari K+ (hanya diinduksi TMT dosis 0,6 mg/kgBB), K- (hanya diberikan Na-CMC 0,5%), P1 (diinduksi TMT dan diberi ekstrak daun pepaya dosis 500 mg/kgBB), P2 (diinduksi TMT dan diberi ekstrak daun pepaya dosis 1000 mg/kgBB), P3 (diinduksi TMT dan diberi ekstrak daun pepaya dosis 2000 mg/kgBB). Parameter yang diamati adalah nekrosis sel piramidal dan persentase kerusakan sel piramidal pada hipokampus dan korteks serebri. Hasil penelitian menunjukkan terjadi penurunan persentase kerusakan sel piramidal di hipokampus pada P1 sebesar 26,46%, P2 sebesar 28,91%, dan P3 sebesar 26,67%. Persentase kerusakan sel piramidal di korteks serebri mengalami penurunan pada P1 sebesar 9,62%, P2 sebesar 11,14%, dan P3 sebesar 14,88%. Dosis optimum ekstrak daun pepaya yang bersifat neuroprotektif adalah dosis 2000 mg/kgBB (P3). Kata kunci: daun pepaya, TMT, neuroprotektif, nekrosis, hipokampus
Hepatoprotektor Rumput Laut Eucheuma cottonii Pada Tikus Putih (Rattus norvegicus L.) Jantan Yang Diinduksi Natrium Nitrit (Nano2) Ida Ayu Ratih Purnama Dewi; Ngurah Intan Wiratmini; Iriani Setyawati
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p12

Abstract

Sodium nitrite (NaNO2) is a food preservative that is often added to processed meat products. Excessive consumption of NaNO2 can be detrimental to health. One effort to minimize the negative effects caused by NaNO2 is by consuming antioxidants. This study aimed to determine the effectiveness of Eucheuma cotonii seaweed extract on improving blood profile, liver and kidney function in male white rats (Rattus norvegicus) induced by NaNO2. This research used a Completely Randomized Design (CRD) consisting of two control groups and three treatment groups. Each group consists of six replications. Negative control (K-) receive aquades and CMC NA 0,5%. Positive control (K +) was only received 22.5 mg/kgBW NaNO2 for 35 days then on the day 36 until the day 45 only given 0,5% CMC Na. The treatment groups P1, P2, and P3 respectively on day 1 to day 15, only received 22.5 mg/kgBW NaNO2 in the morning, and then day 16 to day 35 received 22.5 mg/kgBW NaNO2 in the morning and received 150 mg/kgBW (P1), 300 mg/kgBW (P2), 450 mg/kgBW (P3) Eucheuma cottoni seaweed extract in the evening. Day 30 to day 45, the treatment groups only received Eucheuma cottoni seaweed extracts at each dose. Blood and liver were collected on day 46. Data analysis of serum alanine transaminase (ALT) and aspartate aminotransferase (AST) enzyme levels and liver histology were performed with Anova statistical test and continued with Duncan post hoc test. The results showed Eucheuma cottonii extract dose of 150 mg / kgBB significantly reduced the level of liver damage seen from the levels of ALT and AST and liver histology (percentage inflammatory cell infiltration, congestion, fatty degeneration and necrosis) in white rats induced by sodium nitrite (NaNO2). Keywords: Preservative, AST, ALT, histopathology, liver
HISTOLOGI TESTIS TIKUS (Rattus norvegicus) JANTAN YANG DIBERI TEPUNG DAUN LAMTORO (Leucaena leucocephala Lamk. de Wit) HASIL PERENDAMAN A.A. Istri Mas Padmiswari; Ngurah Intan Wiratmini; I Wayan Kasa
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p07

Abstract

Lamtoro merupakan salah satu tanaman yang memiliki kandungan protein yang cukup tinggi yaitu berkisar antara 25-35%. Namun, pemanfaatan lamtoro menjadi terbatas karena mengandung zat antinutrisi seperti mimosin. Kandungan mimosin dapat diturunkan melalui beberapa metode salah satunya adalah melalui perendaman dalam air. Penelitian ini bertujuan untuk mengetahui pengaruh dari pemberian tepung daun lamoro hasil perendaman (TDLP) terhadap histologi testis tikus jantan. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) yang terdiri dari empat kelompok dengan masing-masing delapan ulangan. Perlakuan berupa pemberian tepung daun lamtoro hasil perendaman yang dicampur dengan pelet komersial dengan aras 100% pakan komersial (tanpa TDLP) sebagai kontrol (P0), 92,5% pakan komersial + 7,5% TDLP sebagai perlakuan 1 (P1), 85% pakan komersial + 15% TDLP sebagai perlakuan 2 (P2) dan 77,5% pakan komersial + 22,5% TDLP sebagai perlakuan 3 (P3). Perlakuan diberikan pada tikus jantan selama 30 hari. Variabel yang diamati pada penelitian ini adalah jumlah sel spermatogenik. Data hasil penelitian diolah menggunakan program statistik komputer (SPSS 16.0 for Windows) dengan menggunakan uji One Way Anova. Hasil penelitian menunjukkan bahwa penambahan TDLP dalam ransum tidak berpengaruh nyata (P>0,05) terhadap jumlah sel spermatogenik. Hal ini menunjukkan bahwa penambahan TDLP hingga aras 22,5% tidak menurunkan jumlah sel spermatogenik.
Kuantifikasi Protein HAS2, PTX3, BCL2 dan BAX dalam Sel Kumulus Oosit Matur (MII) dan Imatur (MI) Pada Pasien Bayi Tabung Ni Putu Sri Risa Dewi; Ngurah Intan Wiratmini; Jaqueline Sudiman
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p01

Abstract

Oocyte developmental competence is one of the determining factors that in?uence the outcomes of an IVF cycle regarding the ability of a female gamete to reach maturation and be fertilized. A non-invasive approach using proteomic analysis of oocyte cumulus cells (CCs) can be used as a method to predict oocyte competence and viability that could potentially function as molecular predictors for IVF program prognosis. Our study was aimed at quantifying protein markers (HAS2, PTX3, BCL2 and BAX) that are expressed in human CCs between mature (MII) and immature (MI) oocytes. This research is an analytic observational study. Twelve samples of CCs from mature (MII) and immature (MI) oocytes were collected after ovum pick up from patients undergoing ICSI. CCs samples were isolated using an ultrasonicator and protein expressions were quantified using ELISA method. The Mann-Whitney test was used to compare the protein expressions from CCs between mature and immature oocytes. The results showed HAS2, PTX3 and BCL2 proteins expressions was increase in CCs from mature oocytes but had no significant effect compared to CCs from immature oocytes, however, BAX protein expression was significantly higher (P<0.05) in CCs from immature oocytes which CCs from mature oocytes. This present study shows that BAX proteins could be used as markers for oocyte quality and maturation. Key words: ELISA, maturation, proteins marker, cumulus cells (CCs)
Co-Authors A.A.S. A. Sukmaningsih AA Istri Mas Padmiswari Anak Agung Gde Raka Dalem Apriliani, Sinta Arnenda, Gussasta Levi Bram Setyadji Dessy Sukma Wirastuti Elfira Dzikri Ashfahani Elrade Rofaani G.A.A Rina Febrina Gussasta Levi Arnenda I Gede Wiranatha I GUSTI AYU MANIK ERMAYANTI I Made Sara Wijana I Made Sara Wijana I Putu Ari Wijana Dipa I Wayan Andi Yoga Kurniawan I Wayan Kasa I.M.A.S. Wijaya Ida Ayu Nopiari Ida Ayu Ratih Purnama Dewi Inna Narayani IRIANI SETYAWATI Iriani Setyawati Jaqueline Sudiman Job Nico Subagio Joko Raharjo, Sentot Joko Wiryatno Luh Putu Wrasiati MADE PHARMAWATI Maria Antonia Margaretha Ferandez Maria Antonia Margaretha Fernandez Mery Wintari Muyasaroh, Chanif Lutfiyati Ni Desak Made Aprilia Dewi Ni Komang Tia Pramesti Ni Luh Eka Setiasih Ni Luh Suriani Ni Made Rai Suarni Ni Made Suartini Ni Putu Adriani Astiti Ni Putu Ayu Dewi Wijayanti Ni Putu Sri Risa Dewi Ni Wayan Adya Puttry Purna Yogini Ni Wayan Sudatri Putu Titah Kawitri Resen Revael Sihombing Salsabillah Rahma SANG KETUT SUDIRGA shanon lestari Sistra, Kanigara Anupama Siti Masnunah Sri Sulastri Sukma Sushanti Sukmaningsih, A. A.S.A. Wahyu Puji Lestari WAHYU WIDOWATI Widia Meliawati