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Trisnowati Budi Ambarningrum
Fakultas Biologi, Universitas Jenderal Soedirman
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A Reproductive Performance of Black Soldier Fly (Hermetia illucens) With Pennisetum purpureum Extract Rifai, Lutfin Agri; Haryanto, Trisno; Ambarningrum, Trisnowati Budi; Hashifah, Fathimah Nurfitri; -, Mutala'liah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 6 No 3 (2024): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2024.6.3.10560

Abstract

Black soldier fly (Hermetia illucens) is one of the biological agents for organic waste decomposition and this process produced insect biomass with significant economic value. Giving of juvenile hormone (JH) which is thought to be present in nappier grass (Pennisetum purpureum) can increase the number of BSF egg production. The purpose of this research is to determine the effect of nappier grass extract on the reproductive performance of H. illucens based on the number of eggs produced, the percentage of eggs hatched, and the sex ratio of BSF flies. The research was conducted experimentally using a completely randomized design (CRD). The number of eggs produced and the percentage of eggs hatched analyzed using non-parametric analysis with the Kruskal-wallis test and followed by the Mann Whitney test. Statistical testing using SPSS version 16.0. The sex ratio of BSF flies was analyzed descriptively. Phytochemical test analysis was carried out to ensure the content of compounds present in nappier grass extract (P. purpureum). The result shows that nappier grass extract has significant effect (P<0.05) on number of eggs produced and the percentage of eggs hatched based on Kruskal-wallis test. Based on the results of the Mann Whitney test, it was found that all treatments of nappier grass extract on the number of BSF egg production and the percentage of eggs hatched was significantly different (P<0.05). The highest egg production effect which the average number of egg production was 209 mg at 600 ppm. At 600 ppm had the highest percentage egg that hatched effect which the average of percentage egg that hatched was 88,759%. The optimum sex ratio for the cultivation was at 200 ppm, which is 60.42% female and 39.58% male Keyword: . Black Soldier Fly, Pennisetum purpureum, Reproductive performance
PENERAPAN TEKNOLOGI PENGOLAHAN SAMPAH ORGANIK DESA SALEM BREBES MENUJU DESA MANDIRI SAMPAH Listiowati, Emyliana; Setyanto, Refius Pradipta; Ambarningrum, Trisnowati Budi; Pamudji, Ganjar; Susanto, Irwan; Lestari, Sri
Jurnal Abdi Insani Vol 12 No 2 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i2.2020

Abstract

Waste management is a global problem in Indonesia. Salem Village is one of the villages in Brebes Regency where waste management is not optimal. The condition of the increasingly piled up waste without processing creates an unpleasant odor and disturbs the health of the surrounding community. The waste processing carried out is burning the existing waste, which adds to the problem of air pollution. As a result of continuous waste burning, the hangar was also burned. The impact of burning waste is very dangerous because it releases monoxide gas which disturbs public health. The purpose of this program is to provide socialization of waste processing, waste banks, Village Regulations on waste management, waste processing technology based on BSF flies and maggots into exoenzyme products that have economic value. Activity methods include lectures and discussions, training, demonstration plots and mentoring. Socialization to cadres and administrators of Bumdes Bina Usaha Salem Village regarding a) independent waste management and waste bank applications; b) socialization of village regulations (perdes) governing waste management, c) dissemination of information about organic waste processing technology based on BSF maggots and Ecoenzymes.The results of the socialization showed an increase in participants' knowledge about the necessity of business management and group management, village regulations governing waste management, the bioecology of BSF maggots, and the technology of organic waste bioconversion using BSF maggots. The training and demonstration plots provided to partners included preparation for waste processing, ecoenzym production, baby maggot maintenance, organic waste processing techniques with BSF maggots, and prepupa harvesting.  The evaluation of the activity showed that the pre-test scores initially ranged from 25 (5.88%) to 75 (5.88%). After the technology transfer, the post-test scores increased to a range of 60 (5.88%) to 85 (11.77%).  The Community Service Program can realize the independence of the residents of Salem village in managing organic waste.
Tingkat Resistensi Lipas Jerman (Blattella germanica L.) asal Tiga Pasar di Kota Purwokerto terhadap Fipronil Menggunakan Metode Kontak dan Umpan: Resistance Level of German Cockroaches (Blattella germanica L.) Origin of Three Traditional Markets in Purwokerto City to Fipronil Ambarningrum, Trisnowati Budi; Kusmintarsih, Endang Srimurni; Haryanto, Trisno; Basuki, Edi; Rejeki, Dwi Sarwani Sri
Aspirator Vol 14 No 1 (2022): Jurnal Aspirator Volume 14 Nomor 1 2022
Publisher : Perkumpulan Entomologi Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/asp.v14i1.4495

Abstract

Abstract. German cockroaches in Indonesia have developed resistance to various insecticides. Although cases of german cockroach resistance have been reported in several areas in Indonesia, there have been no reports of such resistance cases in Purwokerto. The specific purpose of this study was to determine the resistance status of german cockroaches from three traditional markets (pasar) in Purwokerto to fipronil. The method used was the contact and bait method, using ten male cockroaches and repeated five times for each strain. The data obtained were analyzed using probit analysis to calculate the time of  death (Lethal Time), and then the resistance level was calculated. The results showed that the time of death (LT50) for field cockroaches using the contact method, the highest resistance level was obtained from the Pasar-1 strain with an LT50 value of 3.05 hours, while the lowest was in the Pasar-3 strain with an LT50 value of 1.83 hours. In testing with the bait method using gel bait containing 0.03% fipronil, the LT50 value of cockroaches from Pasar-1 was 14.16 hours, while cockroaches from Pasar-3 had an LT50 value of 8.02 hours. The resistance ratio value (RR50) calculation showed that all cockroaches from three traditional markets in the city of Purwokerto did not show resistance to fipronil which was tested with the resistance ratio value of all field strains below 1. Susceptible to insecticides with the active ingredient fipronil. The active ingredient fipronil in bait formulations may be used for monitoring and control of German cockroaches. Abstrak. Lipas jerman telah resisten terhadap berbagai macam insektisida, dibuktikan dengan adanya kasus resistensi lipas jerman yang dilaporkan terjadi di beberapa wilayah di Indonesia. Meskipun demikian, di wilayah Kota Purwokerto sampai saat ini belum ada laporan mengenai kasus resistensi tersebut. Tujuan khusus penelitian ini adalah mengetahui status resistensi lipas jerman dari tiga pasar tradisional yang ada di Kota Purwokerto terhadap fipronil. Metode yang digunakan adalah metode kontak dan umpan dengan menggunakan sepuluh ekor lipas jantan dan diulang lima kali untuk masing-masing strain. Data yang diperoleh dianalisis menggunakan analisis probit untuk menghitung waktu kematian (Lethal Time), untuk kemudian dihitung tingkat resistensinya. Hasil penelitian menunjukkan bahwa waktu kematian (LT50) lipas lapangan menggunakan metode kontak pada tingkat resistensitertinggi adalah dari strain Pasar-1 dengan nilai LT50 sebesar 3,05 jam, sedangkan terendah adalah strain Pasar-3 dengan nilai LT50 sebesar 1,83 jam. Hasil pengujian dengan metode umpan menggunakan gel bait mengandung fipronil 0.03% diperoleh nilai LT50 lipas asal Pasar-1 sebesar 14,16 jam, sedangkan lipas dari Pasar-3 mempunyai nilai LT50 sebesar 8,02 jam. Hasil penghitungan nilai rasio resistensi (RR50) menunjukkan bahwa semua lipas asal tiga pasar tradisional di Kota Purwokerto tidak menunjukkan resistensi terhadap fipronil yang diujikan dengan nilai rasio resistensi semua strain lapangan di bawah 1. Simpulan hasil penelitian ini adalah lipas strain pasar tradisional di Kota Purwokerto masih rentan terhadap insektisida berbahan aktif fipronil. Bahan aktif fipronil dalam formulasi umpan dimungkinkan untuk digunakan dalam monitoring dan pengendalian lipas jerman.
Effectiveness of Length and Weight Growth of Tenebrio molitor L. Larvae with Plastic Waste Feeding Hashifah, Fathimah Nurfithri; Muslihah, Niken Istikhari; Khafida, Wilda; Haryanto, Trisno; Ambarningrum, Trisnowati Budi
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7665

Abstract

Inorganic waste accumulating in plastic waste, including styrofoam, which has accumulated in large quantities in waste disposal sites (TPS), is challenging to decompose into the environment. Black beetles (Tenebrio molitor) are insects that decompose inorganic waste and can decompose polystyrene waste. The presence of bacteria in the digestion of insects contributes to the decomposition of plastic waste in the form of styrofoam. This study aimed to determine how effective the larvae of T. molitor are in degrading polystyrene waste (styrofoam) through larval development from the process of nine instar stages. In order to develop more prospective larvae, the research was conducted in multiple stages, including initial maintenance and treatment as well as data gathering to test the growth of larval length. The results are known from the length of the larval body, and it is hoped that the larvae can later be used to help decompose small to large-scale styrofoam waste discharged into the environment. Larvae of T. molitor can eat inorganic materials like styrofoam and organic ones like bran. The ability to do this is demonstrated by the growth of the larvae's weight and length, with the rise in larval length in each treatment having the most beneficial result.
Mortalitas Kecoak Jerman Blattella germanica L. (Dictyoptera: Blattellidae) yang terdedah Biopestisida BIO-P60 berbasis Bakteri Pseudomonas fluorescens P60 Lestari, Wahyu Puji; Yulianti, Dwiana Muflihah; Ambarningrum, Trisnowati Budi
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 4 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.4.16382

Abstract

Kecoak merupakan salah satu vektor yang berada di lingkungan permukiman yang dapat menyebarkan berbagai mikroorganisme penyebab penyakit. Salah satunya yaitu kecoak Jerman (Blattella germanica). Kecoak Jerman perlu dikendalikan dengan metode yang lebih aman. Penelitian ini bertujuan untuk mengetahui mortalitas kecoak Jerman yang terdedah biopestisida BIO-P60. Penelitian dilakukan dengan menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL). Perlakuan terdiri dari umpan yang mengandung biopestisida BIO-P60 dengan konsentrasi 60, 65, 70, 75, 80, 85, dan 90% serta kontrol positif menggunakan fipronil 0,05% dan kontrol negatif hanya menggunakan umpan tanpa insektisida. Masing-masing perlakuan diulang tiga kali. Pengamatan mortalitas dilakukan selama enam hari. Hasil penelitian menunjukkan terdapat perbedaan nyata antara perlakuan BIO-P60 pada konsentrasi 75-90% dengan kontrol negatif (p≤0.05). BIO-P60 konsentrasi 75-90% dapat menyebabkan mortalitas ≥50% dalam waktu enam hari, sedangkan kontrol fipronil dapat mencapai mortalitas 100% dalam waktu 16 jam. Kata kunci : biopestisida, BIO-P60, kecoak Jerman, mortalitas, Pseudomonas fluorescens P60.
Karakteristik Bakteri Pendegradasi Bahan Pangan Asal Saluran Pencernaan Kecoa Amerika (Periplaneta americana) dari Pasar Tradisional Dwiana Muflihah Yulianti; Arif Rahman Hikam; Trisnowati Budi Ambarningrum; Taruna Dwi Satwika; Dyah Fitri Kusharyati; Hilmiyah Al'alawiyah
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1662

Abstract

American cockroach (Periplaneta americana) is one of the most common pests found in houses, apartments, and public facilities such as markets. As vector insects, cockroaches have symbiosis with microorganisms. Some of these microorganisms can live in cockroaches' cuticles and digestive tracts. Besides being able to pose a danger to human health, the microbes in cockroach bodies also have the potential to cause food damage. This study aimed to determine the diversity of microorganisms originating from the digestive tract of the American cockroach and their potential to cause food spoilage. The research was conducted in several stages: sampling cockroaches at a traditional market, isolating bacteria from the digestive tract of cockroaches, qualitatively testing amylolytic, proteolytic, and lipolytic potentials, and characterizing bacterial isolate by morphologically and biochemically character. The results showed that there were 26 isolates of bacteria isolated from the digestive tract of American cockroaches from the market, 62.96% of the isolates were found to have the ability to degrade protein, 37.04% of the isolates had the ability to degrade starch, and 40.74% of the total isolates can degrade fat. These isolates had various morphological and biochemical characteristics. Bacterial isolates derived from American cockroaches from traditional markets have the potential to degrade food.
Manipulasi Temperatur Terhadap Perkembangan Sex Ratio Black Shoulder Fly (Hermetia illucens) Trisno Haryanto; Nabila Putri Effendi; Trisnowati Budi Ambarningrum; Eko Setiyono; Hery Pratiknyo
Biotropic : The Journal of Tropical Biology Vol. 8 No. 1 (2024): Biotropic, Volume 8 Nomor 1, 2024
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v8i1.2063

Abstract

Temperature is one of the factors that influences the life cycle of Hermetia illucens. This research aimed to determine the effect of temperature differences stimulated at the larval stage on the sex ratio of H. illucens. Knowledge of the correct use of temperature can be used as a reference in cultivating H. illucens. The research design used a Completely Randomized Design (CRD) with temperature variations (27oC; 30oC : 36oC). The variables observed included the sex ratio of H. illucens. Data were analyzed using one way ANOVA at a significance level of 95%. The research results show that the optimal sex ratio for cultivation is at room temperature (24°-31°C), namely 55% females and 45% males. The 27°C temperature treatment had the highest number of female, 70.83%, while the number of male was the highes. found at a temperature of 30°C, 62.50%.
Co-Authors -, Mutala'liah Aliefia, Rizky Arjunnajat Alvira Rifdah Sativa Annisa, Dyah Retno Arif Rahman Hikam Atang Atang Atang, Atang Bambang Heru Budianto Darsono Darsono Dwi Iva Fitriana Dwi Sarwani Sri Rejeki Dwiana Muflihah Yulianti Dyah Fitri Kusharyati Dyah Retno Annisa Edi Basuki Edi Basuki Edi Basuki Edy Riwidiharso Eko Setiyono Emyliana Listiowati, Emyliana ENDANG SRIMURNI KUSMINTARSIH Endang Srimurni Kusmintarsih Endang Srimurni Kusmintarsih ENDANG SRIMURNI KUSMINTARSIH ENDANG SRIMURNI KUSMINTARSIH Femilia Hajar Ilhami Fitriana, Dwi Iva Haryanto, Trisno Hashifah, Fathimah Nurfithri Hashifah, Fathimah Nurfitri Hery Pratiknyo Hery Pratiknyo Hery Pratiknyo Hery Pratiknyo Hilmiyah Al'alawiyah Ika Rakhmayani Ilhami, Femilia Hajar Intan Ahmad Irma Fatmanintyas Irwan Susanto Khafida, Wilda Kustiati Kustiati Maula, Riska Isna Muslihah, Niken Istikhari Nabila Putri Effendi Nirwani, Shintya Nitra Nuranisah, Annanda Pamudji, Ganjar Rakhmayani, Ika Rifai, Lutfin Agri Riska Isna Maula Rizky Arjunnajat Aliefia Rokhmani Rokhmani Sativa, Alvira Rifdah Setiyono, Eko Setyanto, Refius Pradipta Setyawati, Endang Ariyani Sri Lestari Sri Lestari Sri Yusmalinar Taruna Dwi Satwika Trisno Haryanto Untung Susilo Wahyu Puji Lestari, Wahyu Puji Yerslin Mantolu Yulianti, Dwiana Muflihah